Isolation and serial propagation of human group C rotaviruses in a cell line (CaCo-2).

abstract：:Parainfluenza virus type 3 (Para 3) is an important childhood pathogen causing a significant amount of bronchiolitis and pneumonia in infants. Virus data were analysed over a twelve-year period (1978-1989), and a peak incidence of infection was observed in spring as in Houston, USA, unlike in the United Kingdom where ...

journal_title：Journal of medical virology

authors： de Silva LM,Cloonan MJ

更新日期：1991-09-01 00:00:00

abstract：:The nucleotide sequence of 10 isolates of human parvovirus B19 (B19) were determined and compared throughout 96.3% of the open reading frames (4145 nucleotides from nt. 509-4653). In the 4145 nucleotides, 122 mutation sites were found, of which 24 were accompanied by amino acid displacement. Furthermore, the polymorph...

journal_title：Journal of medical virology

authors： Fukada K,Matumoto K,Takakura F,Yamaki M,Sato H,Okochi K,Maeda Y

更新日期：2000-09-01 00:00:00

abstract：:Enterovirus 68 (EV-D68) was associated with mild to severe respiratory infections. In the last 4 years, circulation of different EV-D68 strains has been documented worldwide. In this study, the phylogenetic characterization of nine EV-D68 strains identified in patients in the 2010-2012 period and 12 additional EV-D68 ...

journal_title：Journal of medical virology

authors： Piralla A,Girello A,Grignani M,Gozalo-Margüello M,Marchi A,Marseglia G,Baldanti F

更新日期：2014-09-01 00:00:00

abstract：:We compared characteristics of patients with GB virus C/hepatitis G virus (GBV-C/HGV) RNA to those of patients with GBV-C/HGV E2-antibody. GBV-C/HGV RNA and GBV-C/HGV antibody were assayed in 83 persons with hemophilia using a reverse transcription-polymerase chain reaction and an enzyme-linked immunosorbent assay, re...

journal_title：Journal of medical virology

authors： Toyoda H,Takahashi I,Fukuda Y,Hayakawa T,Takamatsu J

更新日期：2000-01-01 00:00:00

abstract：:Occult HBV infection is defined as the persistence of HBV DNA in individuals negative for HBV surface antigen (HBsAg), and many different mechanisms have been reported in different countries. However, in China, one of the endemic areas for HBV infection, no reports have been published on occult HBV infection. The pres...

journal_title：Journal of medical virology

authors： Fang Y,Teng X,Xu WZ,Li D,Zhao HW,Fu LJ,Zhang FM,Gu HX

更新日期：2009-05-01 00:00:00

abstract：:The impact of cytomegalovirus (CMV) infection of the genital tract during pregnancy on adverse pregnancy outcomes is not understood fully. A real-time PCR assay was used to determine vaginal shedding of CMV in 993 healthy pregnant Japanese women and the results were compared with the outcome of pregnancy. CMV DNA was ...

journal_title：Journal of medical virology

authors： Tanaka K,Yamada H,Minami M,Kataoka S,Numazaki K,Minakami H,Tsutsumi H

更新日期：2006-06-01 00:00:00

abstract：:The rapid spread of the epidemic has aroused widespread concern in the international community. Severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) was first reported in China, with bats as the likely original hosts and pangolins as potential intermediate hosts. The current source of the disease is mainly pat...

journal_title：Journal of medical virology

authors： Ye Q,Wang B,Mao J,Fu J,Shang S,Shu Q,Zhang T

更新日期：2020-07-01 00:00:00

abstract：:Major differences exist between HIV-1 and HIV-2 in terms of epidemiology, pathogenicity, sensitivity to antiretrovirals. Determining the type of HIV infecting a patient is essential for management. The aim of this study was to evaluate the ability of simple/rapid tests to differentiate between HIV-1 and/or HIV-2 infec...

journal_title：Journal of medical virology

authors： Gautheret-Dejean A,Bocobza J,Brunet S,Damond F,Plantier JC,Barin F

更新日期：2015-12-01 00:00:00

abstract：:Sera from 1,943 individuals from Auckland, New Zealand, were tested for the presence of serum antibodies to human T cell lymphotropic virus I (HTLV I), mainly with an enzyme-linked immunosorbent assay (ELISA) with cell extracts as target antigen. The individuals tested were blood donors and mostly Caucasian, but inclu...

journal_title：Journal of medical virology

authors： Reddy D,Okochi K,Woodfield DG,Judd W

更新日期：1987-08-01 00:00:00

abstract：:We developed an immunoperoxidase staining test to detect structural antigens of BK virus (BKV) in Vero cell cultures. This test was used to examine the neutralizing activity of human and immunized animal sera. It was shown that sera positive for BKV antibodies measured by hemagglutination inhibition test and enzyme-li...

journal_title：Journal of medical virology

authors： Flaegstad T,Traavik T,Christie KE,Joergensen J

更新日期：1986-07-01 00:00:00

abstract：:In addition to the serotype-specific primers described previously (1 to 7), a new serotype 8-specific primer has been designed, allowing detection of all astrovirus serotypes. A total of 1,382 diarrheal stool samples in 5 regions in Japan were examined by reverse transcription-polymerase chain reaction (RT-PCR). The i...

journal_title：Journal of medical virology

authors： Sakamoto T,Negishi H,Wang QH,Akihara S,Kim B,Nishimura S,Kaneshi K,Nakaya S,Ueda Y,Sugita K,Motohiro T,Nishimura T,Ushijima H

更新日期：2000-07-01 00:00:00

abstract：:To characterize the state of dual infection with hepatitis B virus (HBV) and hepatitis C virus (HCV), HBV-DNA and HCV-RNA levels were determined by spot hybridization or polymerase chain reaction in the sera of patients who were positive for both hepatitis B surface antigen and HCV antibody. Among 27 patients who show...

journal_title：Journal of medical virology

authors： Koike K,Yasuda K,Yotsuyanagi H,Moriya K,Hino K,Kurokawa K,Iino S

更新日期：1995-02-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay (ELISA) using recombinant nucleoprotein (rNP) was reported for the detection of immunoglobulin G (IgG) antibodies to Crimean-Congo hemorrhagic fever (CCHF) virus (CCHFV). The immunoglobulin M (IgM)-capture ELISA was developed for the diagnosis of CCHFV infections, using CCHFV rNP a...

journal_title：Journal of medical virology

authors： Saijo M,Tang Q,Shimayi B,Han L,Zhang Y,Asiguma M,Tianshu D,Maeda A,Kurane I,Morikawa S

更新日期：2005-02-01 00:00:00

abstract：:Hepatitis B virus (HBV) transgenic mice that replicate HBV in the liver generally do not exhibit gross liver pathology, while maintaining a high level (10(7) or greater) of viral titer in the blood. We have used this model to determine the minimum effects of HBV replication in the liver on cellular gene transcription,...

journal_title：Journal of medical virology

authors： Hajjou M,Norel R,Carver R,Marion P,Cullen J,Rogler LE,Rogler CE

更新日期：2005-09-01 00:00:00

abstract：:Hepatitis C Virus (HCV) NS3 protease is an attractive target for antiviral agent development because it is required for viral replication. Because a stable cell culture system or small animal model to study HCV replication is not readily available, we constructed an in vitro model allowing the investigation of NS3 tra...

journal_title：Journal of medical virology

authors： Heintges T,Encke J,zu Putlitz J,Wands JR

更新日期：2001-12-01 00:00:00

abstract：:In earlier studies, hepatitis E virus (HEV) particles were detected in the stools of patients with enterically transmitted non-A, non-B (ENANB) hepatitis, and HEV was etiologically associated with this disease. Such particles have not been observed in the liver, however. We describe the pathological findings in the li...

journal_title：Journal of medical virology

authors： Asher LV,Innis BL,Shrestha MP,Ticehurst J,Baze WB

更新日期：1990-07-01 00:00:00

abstract：:Chikungunya fever is a viral disease transmitted to humans by the bite of infected mosquitoes. The disease is characterized by fever, headache, rash, severe joint, and muscle pain. To evaluate the disease burden in the population and the effectiveness of public health measures, periodic seroprevalence surveys are esse...

journal_title：Journal of medical virology

authors： Shilpa C,Kavitha K,Sudheesh N,Sabeena S,Prasad V,Hindol M,Arunkumar G

更新日期：2020-01-01 00:00:00

abstract：:The detection of hepatitis B virus (HBV) DNA plays a critical role in determining the level of viral replication in HBV-infected patients. However, how to select appropriate HBV DNA detection method, low-sensitivity (ls) and hypersensitivity (hs) remains unclear. In this study, hepatitis B surface antigen (HBsAg), hep...

journal_title：Journal of medical virology

authors： Liu C,Wu W,Shang S,Huang E,Xun Z,Lin J,Chen T,Yang B,Chen J,Ou Q

更新日期：2019-02-01 00:00:00

abstract：:Complete coding regions were sequenced for two new enterovirus genomes: EV-B93 previously identified by VP1 sequencing, derived from a child with acute flaccid paralysis in the Democratic Republic of Congo; and EV-C95 from a French soldier with acute gastroenteritis in Djibouti. The EV-B93 P1 had more than 30% nucleot...

journal_title：Journal of medical virology

authors： Junttila N,Lévêque N,Magnius LO,Kabue JP,Muyembe-Tamfum JJ,Maslin J,Lina B,Norder H

更新日期：2015-03-01 00:00:00

abstract：:Rotavirus was detected in the stools of five children stricken with sudden infant death syndrome (SIDS) over a three-week period. While none of the children had acute gastroenteritis, four of the five had acute upper respiratory infections. Rotavirus was also identified in tracheal aspirates from two of the infants. E...

journal_title：Journal of medical virology

authors： Yolken R,Murphy M

更新日期：1982-01-01 00:00:00

abstract：:An anti-hepatitis A virus (HAV)-specific immunoglobulin M capture enzyme-linked immunosorbent assay (anti-HAV IgM ELISA) kit was re-designed for laboratory use and compared with a commercial anti-HAV IgM detection system using 58 serum samples collected from patients, vaccines, and healthy individuals. Because concord...

journal_title：Journal of medical virology

authors： Kiyohara T,Ouchi Y,Hasegawa Y,Sato T,Yoneyama T,Ishii K,Ito T,Wakita T

更新日期：2009-09-01 00:00:00

abstract：:Analysis of genetic variation in 16 varicella-zoster virus (VZV) isolates selected at random and circulating in the Irish population between March 2002 and February 2003 was carried out. A 919 bp fragment of the glycoprotein E gene (open reading frame 68) encompassing codon 150, at which a non-synonymous mutation defi...

journal_title：Journal of medical virology

authors： Carr MJ,McCormack GP,Crowley B

更新日期：2004-05-01 00:00:00

abstract：:The JC virus (JCV) is a common human virus persisting in renal tissue. In immunocompromised individuals it may reactivate and cause progressive multifocal leukoencephalopathy (PML). JCV has also been implicated in cancerogenesis, leading to various brain tumors and cancers of gastrointestinal tract. In this study the ...

journal_title：Journal of medical virology

authors： Kmieciak D,Debicki S,Trzeciak WH

更新日期：2008-06-01 00:00:00

abstract：:The multiplication of HSV-1 and HSV-2 strains in the heart and the corresponding dorsal root ganglia (DRG) was examined in experimentally infected mice. Infectious HSV-1 was recovered from the heart between the second and fourth day after inoculation and 3 days later from the DRG. Both the heart and DRG yielded infect...

journal_title：Journal of medical virology

authors： Grodums EI,Kuling PJ,Zbitnew A

更新日期：1981-01-01 00:00:00

abstract：:A nationwide hepatitis B vaccination program was launched in Taiwan in 1984. To study the impact of this ongoing program on hepatitis B virus (HBV) infection, a follow-up seroepidemiologic study was carried out in 1989 in a Taipei district where pre-vaccination seroepidemiology had been studied. HBV markers were studi...

journal_title：Journal of medical virology

authors： Tsen YJ,Chang MH,Hsu HY,Lee CY,Sung JL,Chen DS

更新日期：1991-06-01 00:00:00

abstract：:Serum specimens from 1,109 individuals at different ages in Beijing, China, were collected between April 1996 and March 1997 and tested for IgG antibodies against human caliciviruses using enzyme immune assays (EIAs). Baculovirus-expressed recombinant Norwalk virus (rNV) and Mexican virus (rMxV) capsid proteins were u...

journal_title：Journal of medical virology

authors： Jing Y,Qian Y,Huo Y,Wang LP,Jiang X

更新日期：2000-01-01 00:00:00

abstract：:The infectivity titer of a standard stock of the SAR-55 strain of hepatitis E virus (HEV) was determined in cynomolgus macaques (Macaca fascicularis) and the effect of dose on the course of the infection was examined by weekly monitoring of alanine aminotransferase (ALT) and anti-HEV levels. Antibody to HEV (anti-HEV)...

journal_title：Journal of medical virology

authors： Tsarev SA,Tsareva TS,Emerson SU,Yarbough PO,Legters LJ,Moskal T,Purcell RH

更新日期：1994-06-01 00:00:00

abstract：:The epidemiology, transmission and clinical symptoms of human parechoviruses [HPeV, classified earlier as enteroviruses; echovirus 22 (HPeV1) and echovirus 23 (HPeV2)] remain poorly characterized. Enteroviruses and one parechovirus species, the Ljungan virus, have been associated with type 1 diabetes in humans and rod...

journal_title：Journal of medical virology

authors： Tauriainen S,Martiskainen M,Oikarinen S,Lönnrot M,Viskari H,Ilonen J,Simell O,Knip M,Hyöty H

更新日期：2007-04-01 00:00:00

abstract：:The importance of hepatitis B virus (HBV) genotypes for disease progression and response to interferon-alpha-based treatment is well established. While almost all patients in the Mediterranean area are infected with HBV genotype D, HBV genotype A is dominant in Northern Europe. However, the distribution of HBV genotyp...

journal_title：Journal of medical virology

authors： Deterding K,Constantinescu I,Nedelcu FD,Gervain J,Nemecek V,Srtunecky O,Vince A,Grgurevic I,Bielawski KP,Zalewska M,Bock T,Ambrozaitis A,Stanczak J,Takács M,Chulanov V,Slusarczyk J,Drazd'áková M,Wiegand J,Cornberg M,

更新日期：2008-10-01 00:00:00

abstract：:Recently a conventional method for the laboratory diagnosis of human cytomegalovirus (HCMV) infection was improved by using centrifugation culture to enhance viral adsorption and by detecting HCMV early antigen and DNA. Comparison of the sensitivity of three rapid methods using commercial diagnostic reagents for the d...

journal_title：Journal of medical virology

authors： Li SB,Fong CK

更新日期：1990-02-01 00:00:00