Abstract：
:The gene for C10, a member of the beta-chemokine family of cytokines, contains a novel second exon encoding a 16 amino acid sequence which is inserted into the amino-terminal region of the protein. We propose a model of C10 structure in which the sequence encoded by the second exon is located on the surface of the protein where it interacts with receptors and defines the predominant epitope of the C10 protein. To test this model we produced C10 protein without the sequence encoded by the second exon, termed C10(-). Antisera generated against purified C10 recognized C10 protein but reacted only weakly with C10(-) protein. Recognition of C10 protein by C10 antisera was not blocked by pre-incubation with a peptide encoding the C10 second exon, indicating that the predominant epitope was not simply the amino acid sequence encoded by the second exon. Antibodies generated against a peptide encoding the C10 second exon sequence recognized C10 on Western blots; this reaction was competed both by C10 second exon peptide and by C10 protein. Functional studies demonstrated that both C10/FLAG and C10(-)/FLAG elicit chemotaxis of mouse PECs and human PBMCs, but that C10(-)/FLAG appears to be more potent than C10/FLAG. Thus these data are consistent with our model of C10 in which the sequence encoded by the second exon is located on the surface, participates in defining the predominant epitope, and plays a key role in recognizing or activating chemokine receptors.
journal_name
Cytokinejournal_title
Cytokineauthors
Berger MS,Taub DD,Orlofsky A,Kleyman TR,Coupaye-Gerard B,Eisner D,Cohen SAdoi
10.1006/cyto.1996.0060subject
Has Abstractpub_date
1996-06-01 00:00:00pages
439-47issue
6eissn
1043-4666issn
1096-0023pii
S1043-4666(96)90060-5journal_volume
8pub_type
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