Tissue engineering scaffolds using superstructures.


:Here, scaffolds as cell and tissue carriers are approached from an engineering point of view, emphasizing material superstructuring in the design of supports. Superstructure engineering provides optimal spatial and nutritional conditions for cell maintenance by the arrangement of structural elements (e.g. pores or fibres) so as to vary the order of cell to cell contact. This approach is illustrated in the design of several scaffolds: knitted fabrics as three-dimensional superstructures for optimized osteosynthesis implants, a new injectable open porous implant system, an angiopolar non-degradable ceramic cell carrier, and an injectable or microsurgically implantable entangled carrier system. The implications for tissue engineering are discussed.






Wintermantel E,Mayer J,Blum J,Eckert KL,Lüscher P,Mathey M




Has Abstract


1996-01-01 00:00:00














  • The influence of substrate stiffness on the behavior and functions of Schwann cells in culture.

    abstract::Solid tissues in the body possess a range of stiffness and provide cells with an instructive microenvironment. Scaffolds in tissue engineering should be rationally designed to become an adhesion substrate friendly to cells. Schwann cells are the principal glial cell in the peripheral nervous system and used as support...


    pub_type: 杂志文章


    authors: Gu Y,Ji Y,Zhao Y,Liu Y,Ding F,Gu X,Yang Y

    更新日期:2012-10-01 00:00:00

  • Vascularized neural constructs for ex-vivo reconstitution of blood-brain barrier function.

    abstract::Ex-vivo blood-brain barrier (BBB) model is of great value for studying brain function and drug development, but it is still challenging to engineer macroscale three-dimensional (3D) tissue constructs to recapitulate physiological and functional aspects of BBB. Here, we describe a delicate 3D vascularized neural constr...


    pub_type: 杂志文章


    authors: Yue H,Xie K,Ji X,Xu B,Wang C,Shi P

    更新日期:2020-07-01 00:00:00

  • The fate of ultrafast degrading polymeric implants in the brain.

    abstract::We have recently reported on an ultrafast degrading tyrosine-derived terpolymer that degrades and resorbs within hours, and is suitable for use in cortical neural prosthetic applications. Here we further characterize this polymer, and describe a new tyrosine-derived fast degrading terpolymer in which the poly(ethylene...


    pub_type: 杂志文章


    authors: Lewitus DY,Smith KL,Shain W,Bolikal D,Kohn J

    更新日期:2011-08-01 00:00:00

  • The promotion of functional recovery and nerve regeneration after spinal cord injury by lentiviral vectors encoding Lingo-1 shRNA delivered by Pluronic F-127.

    abstract::Lingo-1 is selectively expressed on both oligodendrocytes and neurons in the central nervous system (CNS) and serves as a key negative regulator of nerve regeneration, implying a therapeutic target for spinal cord injury (SCI). Here we described a strategy to knock-down Lingo-1 expression in vivo using lentiviral vect...


    pub_type: 杂志文章


    authors: Wu HF,Cen JS,Zhong Q,Chen L,Wang J,Deng DY,Wan Y

    更新日期:2013-02-01 00:00:00

  • Remote and reversible control of in vivo bacteria clustering by NIR-driven multivalent upconverting nanosystems.

    abstract::Achieving remote and reversible control of bacterial cell-cell interactions associated with interference with pathological processes in living systems remains a challenge owing to the complexity of the in vivo microenvironment and the lack of regulation systems. We present, for the first time, the development of a ver...


    pub_type: 杂志文章


    authors: Li W,Dong K,Wang H,Zhang P,Sang Y,Ren J,Qu X

    更新日期:2019-10-01 00:00:00

  • Lipid-based nanodelivery approaches for dopamine-replacement therapies in Parkinson's disease: From preclinical to translational studies.

    abstract::The incidence of Parkinson's disease (PD), the second most common neurodegenerative disorder, has increased exponentially as the global population continues to age. Although the etiological factors contributing to PD remain uncertain, its average incidence rate is reported to be 1% of the global population older than ...


    pub_type: 杂志文章,评审


    authors: Karthivashan G,Ganesan P,Park SY,Lee HW,Choi DK

    更新日期:2020-02-01 00:00:00

  • The performance of gamma- and EtO-sterilised UHMWPE acetabular cups tested under severe simulator conditions. Part 2: wear particle characteristics with isolation protocols.

    abstract::Ultra-high molecular-weight polyethylene (UHMWPE) has been used in total joint replacement for the last three decades. Despite the recent advancements in prosthesis design, the wear of UHMWPE remains a serious clinical problem; the release of wear debris may induce osteolysis and implant loosening. Controlling the qua...


    pub_type: 杂志文章


    authors: Affatato S,Bersaglia G,Emiliani D,Foltran I,Taddei P,Reggiani M,Ferrieri P,Toni A

    更新日期:2003-10-01 00:00:00

  • Redox-responsive nanocapsules for intracellular protein delivery.

    abstract::Direct delivery of proteins to the cytosol of cells holds tremendous potential in biological and medical applications. Engineering vehicles for escorting proteins to the cytosol in a controlled release fashion has thus generated considerable interest. We report here the preparation of redox-responsive single-protein n...


    pub_type: 杂志文章


    authors: Zhao M,Biswas A,Hu B,Joo KI,Wang P,Gu Z,Tang Y

    更新日期:2011-08-01 00:00:00

  • Theranostic liposomes of TPGS coating for targeted co-delivery of docetaxel and quantum dots.

    abstract::The aim of this work was to develop a new type of D-alpha-tocopheryl polyethylene glycol 1000 succinate mono-ester (TPGS) coated multi-functional (theranostic) liposomes, which contain both docetaxel and quantum dots (QDs) for cancer imaging and therapy. Non-targeting and folate receptor targeting TPGS coated theranos...


    pub_type: 杂志文章


    authors: Muthu MS,Kulkarni SA,Raju A,Feng SS

    更新日期:2012-04-01 00:00:00

  • Cell recruitment and transfection in gene activated collagen matrix.

    abstract::Realization of systems able to both recruit cells and influence their fate (affecting their processes) represents a new approach for tissue regeneration. We investigated the potency of gene activated matrix (GAM) and implemented the GAM strategy in order to achieve a control of gene expression, as well as a specific c...


    pub_type: 杂志文章


    authors: Orsi S,De Capua A,Guarnieri D,Marasco D,Netti PA

    更新日期:2010-01-01 00:00:00

  • Development of next generation adeno-associated viral vectors capable of selective tropism and efficient gene delivery.

    abstract::Virus-based nanoparticles have shown promise as vehicles for delivering therapeutic genes. However, the rational design of viral vectors that enable selective tropism towards particular types of cells and tissues remains challenging. Here, we explored structural-functional relationships of the adeno-associated virus 2...


    pub_type: 杂志文章


    authors: Zhang C,Yao T,Zheng Y,Li Z,Zhang Q,Zhang L,Zhou D

    更新日期:2016-02-01 00:00:00

  • Towards autotrophic tissue engineering: Photosynthetic gene therapy for regeneration.

    abstract::The use of artificial tissues in regenerative medicine is limited due to hypoxia. As a strategy to overcome this drawback, we have shown that photosynthetic biomaterials can produce and provide oxygen independently of blood perfusion by generating chimeric animal-plant tissues during dermal regeneration. In this work,...


    pub_type: 杂志文章


    authors: Chávez MN,Schenck TL,Hopfner U,Centeno-Cerdas C,Somlai-Schweiger I,Schwarz C,Machens HG,Heikenwalder M,Bono MR,Allende ML,Nickelsen J,Egaña JT

    更新日期:2016-01-01 00:00:00

  • Antibody conjugated supported lipid bilayer for capturing and purification of viable tumor cells in blood for subsequent cell culture.

    abstract::Interest in the identification and isolation of circulating tumor cells (CTCs) has been growing since the introduction of CTCs as an alternative to the tumor tissue biopsy, which can potentially be important indices for prognosis and cancer treatment. However, the contamination of non-specific binding of normal hemato...


    pub_type: 杂志文章


    authors: Wu JC,Tseng PY,Tsai WS,Liao MY,Lu SH,Frank CW,Chen JS,Wu HC,Chang YC

    更新日期:2013-07-01 00:00:00

  • Development of a soft tissue seal around bone-anchored transcutaneous amputation prostheses.

    abstract::Conventional amputation prosthetics are problematic because they rely on the stump-socket interface for attachment. Intraosseous transcutaneous amputation prostheses (ITAP) could solve these problems; however they rely on the integrity of the soft tissue-implant interface as a barrier to exogenous agents, and in the p...


    pub_type: 杂志文章


    authors: Pendegrass CJ,Goodship AE,Blunn GW

    更新日期:2006-08-01 00:00:00

  • Measurement of the fracture toughness of some contact lens hydrogels.

    abstract::The fracture toughness of three polymethacrylate contact lens hydrogel materials was measured using the method of Generalized Fracture Mechanics. One hydrogel (SNOFLEX S68) had a significantly higher fracture energy per unit area (Jc) than the others, probably because it undergoes plastic deformation at high strains. ...


    pub_type: 杂志文章


    authors: Jackson AP

    更新日期:1990-08-01 00:00:00

  • Imaging specificity of MR-optical imaging agents following the masking of surface charge by poly(ethylene glycol).

    abstract::The coupling of specific antibodies to imaging agents often improves imaging specificity. However, free amine groups designed for the coupling can cause nonspecific binding of the imaging agents. We report here development of a nanocarrier, MnMEIO-silane-NH2-mPEG nanoparticles (NPs), consisting of a manganese-doped ir...


    pub_type: 杂志文章


    authors: Wu SC,Lin KL,Wang TP,Tzou SC,Singh G,Chen MH,Cheng TL,Chen CY,Liu GC,Lee TW,Hu SH,Wang YM

    更新日期:2013-05-01 00:00:00

  • Molecular barriers to biomaterial thrombosis by modification of surface proteins with polyethylene glycol.

    abstract::For cardiovascular biomaterials, thrombosis, thromboembolism and vascular graft occlusion are believed to be precipitated by the adsorption of proteins containing adhesive ligands for platelets. Polyethylene-glycol-diisocyanate (PEG-diisocyanate, 3400 MW) may potentially react with protein amines to form molecular bar...


    pub_type: 杂志文章


    authors: Deible CR,Petrosko P,Johnson PC,Beckman EJ,Russell AJ,Wagner WR

    更新日期:1998-10-01 00:00:00

  • Secretory function of adrenal chromaffin cells cultured on polypyrrole films.

    abstract::Polypyrrole (PPy) is a conducting polymer and is obtained electrochemically on an electrode such as indium-tin oxide (ITO). In this study, in order to develop a novel cell-culture system which makes it possible to communicate with cultured mammalian cells, bovine adrenal chromaffin cells were cultured on PPy-coated IT...


    pub_type: 杂志文章


    authors: Aoki T,Tanino M,Sanui K,Ogata N,Kumakura K

    更新日期:1996-10-01 00:00:00

  • Molecular modulated cysteine-selective fluorescent probe.

    abstract::We have synthesized a series of coumarins (1-3) that can emit fluorescence in a turn-on manner through a Michael-type reaction with thiol-containing compounds. The only difference among the coumarins is the position of a carboxyl group on its benzene ring moiety near the double-bond conjugated coumarin. Their selectiv...


    pub_type: 杂志文章


    authors: Jung HS,Pradhan T,Han JH,Heo KJ,Lee JH,Kang C,Kim JS

    更新日期:2012-11-01 00:00:00

  • Novel delivery system based on complexation hydrogels as delivery vehicles for insulin-transferrin conjugates.

    abstract::A variety of approaches have been investigated to address the problems associated with oral insulin delivery, but the bioavailability of oral insulin is still low. Insulin is rapidly degraded by the enzymes in the GI tract and is not transported across the epithelial barrier easily. The oral insulin formulation develo...


    pub_type: 杂志文章


    authors: Kavimandan NJ,Losi E,Peppas NA

    更新日期:2006-07-01 00:00:00

  • The use of PEGylated poly [2-(N,N-dimethylamino) ethyl methacrylate] as a mucosal DNA delivery vector and the activation of innate immunity and improvement of HIV-1-specific immune responses.

    abstract::To minimize the cytotoxicity of poly (2-(dimethylamino) ethyl methacrylate) (PDMAEMA) as a gene delivery vector, we synthesized PEGylated PDMAEMA by atom transfer radical polymerization (ATRP). Here we report its effects on transfection efficiency in vitro delivered with a GFP expression plasmid and immunogenicity in ...


    pub_type: 杂志文章


    authors: Qiao Y,Huang Y,Qiu C,Yue X,Deng L,Wan Y,Xing J,Zhang C,Yuan S,Dong A,Xu J

    更新日期:2010-01-01 00:00:00

  • The effect of collagen-targeting platelet-derived growth factor on cellularization and vascularization of collagen scaffolds.

    abstract::Platelet-derived growth factor BB (PDGF-BB) was an important inductive factor during wound healing, but the lack of efficient delivery system limited its clinical application. Here, a peptide of seven amino acids was firstly utilized to engineer PDGF-BB to construct a collagen-targeting system. This peptide functioned...


    pub_type: 杂志文章


    authors: Lin H,Chen B,Sun W,Zhao W,Zhao Y,Dai J

    更新日期:2006-11-01 00:00:00

  • Differential and synergistic effects of mechanical stimulation and growth factor presentation on vascular wall function.

    abstract::We investigated the hypothesis that immobilizing TGF-β1 within fibrin hydrogels may act in synergy with cyclic mechanical stimulation to enhance the properties of vascular grafts. To this end, we engineered a fusion TGF-β1 protein that can covalently anchor to fibrin during polymerization upon the action of factor XII...


    pub_type: 杂志文章


    authors: Liang MS,Koobatian M,Lei P,Swartz DD,Andreadis ST

    更新日期:2013-10-01 00:00:00

  • The effects of combined micron-/submicron-scale surface roughness and nanoscale features on cell proliferation and differentiation.

    abstract::Titanium (Ti) osseointegration is critical for the success of dental and orthopedic implants. Previous studies have shown that surface roughness at the micro- and submicro-scales promotes osseointegration by enhancing osteoblast differentiation and local factor production. Only relatively recently have the effects of ...


    pub_type: 杂志文章


    authors: Gittens RA,McLachlan T,Olivares-Navarrete R,Cai Y,Berner S,Tannenbaum R,Schwartz Z,Sandhage KH,Boyan BD

    更新日期:2011-05-01 00:00:00

  • Excess polycation mediates efficient chitosan-based gene transfer by promoting lysosomal release of the polyplexes.

    abstract::The optimal ratio of the polycation's amine to DNA phosphate group (N:P) for efficient polymer-based transfection always employs excess polycation versus DNA. Most of the excess polycation remains free in solution, unassociated with the polyplexes, but is essential for efficient transfection. The mechanism by which ex...


    pub_type: 杂志文章


    authors: Thibault M,Astolfi M,Tran-Khanh N,Lavertu M,Darras V,Merzouki A,Buschmann MD

    更新日期:2011-07-01 00:00:00

  • Sequence-specific nuclease-mediated release of cells tethered by oligonucleotide phospholipids.

    abstract::Single-stranded oligonucleotide-conjugated lipids (ssDNA-PEG-lipids) that associate with the cell membrane confer to the cell an artificial adhesive capability via sequence-specific hybridization to complementary oligonucleotides, forming bonds of double stranded oligonucleotides (dsDNA). Such artificial tethers permi...


    pub_type: 杂志文章


    authors: Hoffecker IT,Takemoto N,Arima Y,Iwata H

    更新日期:2015-01-01 00:00:00

  • Biodistribution of PEG-modified gold nanoparticles following intratracheal instillation and intravenous injection.

    abstract::Besides toxicity tests, biokinetic studies are a fundamental part of investigations to evaluate a safe and sustainable use of nanoparticles. Today, gold nanoparticles (Au NPs) are known to be a versatile tool in different areas such as science, engineering or medicine. In this study, we investigated the biokinetics af...


    pub_type: 杂志文章


    authors: Lipka J,Semmler-Behnke M,Sperling RA,Wenk A,Takenaka S,Schleh C,Kissel T,Parak WJ,Kreyling WG

    更新日期:2010-09-01 00:00:00

  • Optimization of 3-D hepatocyte culture by controlling the physical and chemical properties of the extra-cellular matrices.

    abstract::Hepatocytes are anchorage-dependent cells sensitive to microenvironment; the control of the physicochemical properties of the extra-cellular matrices may be useful to the maintenance of hepatocyte functions in vitro for various applications. In a microcapsule-based 3-D hepatocyte culture microenvironment, we could con...


    pub_type: 杂志文章


    authors: Ng S,Wu YN,Zhou Y,Toh YE,Ho ZZ,Chia SM,Zhu JH,Mao HQ,Yu H

    更新日期:2005-06-01 00:00:00

  • Tissue responses of calcium phosphate cement: a study in dogs.

    abstract::The in vivo properties of a new kind of calcium phosphate cement were investigated in this study. Calcium phosphate cement was implanted as paste into femoral bone and dorsal muscle of dogs for 3 and 6 months, and as prehardened form into thigh muscles of dogs for 1, 2 and 6 months. Histology was performed on thin un-...


    pub_type: 杂志文章


    authors: Yuan H,Li Y,de Bruijn JD,de Groot K,Zhang X

    更新日期:2000-06-01 00:00:00

  • The rate of change of pH of lactic acid exposed to glass-ionomer dental cements.

    abstract::The rate of change of pH of aqueous lactic acid at pH 4.2-4.5 (i.e. a little below that of active caries in vivo) in contact with disks of various commercial glass-ionomer cements has been determined in two configurations. In the first of them, a thin film set-up, 20 microl of solution was spread across the surface of...


    pub_type: 杂志文章


    authors: Nicholson JW,Aggarwal A,Czarnecka B,Limanowska-Shaw H

    更新日期:2000-10-01 00:00:00