Abstract：
:We investigated the biological response of human pluripotent stem cells (hPSCs) cultured on a carbon nanotube (CNT) array-based substrate with the long term goal to direct hPSC germ layer specification for a wide variety of tissue engineering applications. CNT arrays were fabricated using a chemical vapor deposition system allowing for control over surface roughness and mechanical stiffness. Our results demonstrated that hPSCs readily attach to hydrophilized and extracellular matrix coated CNT arrays. hPSCs cultured as colonies in conditions supporting self-renewal demonstrated the morphology and marker expression of undifferentiated hPSCs. Conditions inducing spontaneous differentiation lead to hPSC commitment to all three embryonic germ layers as assessed by immunostaining and RT-PCR analysis. Strikingly, the physical characteristics of CNT arrays favored mesodermal specification of hPSCs. This is contradictory to the behavior of hPSCs on traditional tissue culture plastic which promotes the development of ectoderm. Altogether, these results demonstrate the potential of CNT arrays to be used in the generation of new platforms that allow for precise control of hPSC differentiation by tuning the characteristics of their physical microenvironment.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Pryzhkova MV,Aria I,Cheng Q,Harris GM,Zan X,Gharib M,Jabbarzadeh Edoi
10.1016/j.biomaterials.2014.03.011subject
Has Abstractpub_date
2014-06-01 00:00:00pages
5098-109issue
19eissn
0142-9612issn
1878-5905pii
S0142-9612(14)00246-4journal_volume
35pub_type
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