Acute and chronic cyclooxygenase blockage in portal-hypertensive rats: influence in nitric oxide biosynthesis.


BACKGROUND & AIMS:Prostacyclin and nitric oxide have been involved in the hyperkinetic syndrome in portal hypertension. The aim of this study was to investigate the relative contribution and possible interaction between prostacyclin and NO in this circulatory abnormality. METHODS:Portal vein-ligated and sham-operated rats received indomethacin and vehicle either on a short-term (5 mg/kg subcutaneously) or long-term basis (5, continuous 7-day infusion with an osmotic minipump). Measurements of arterial pressure and superior mesenteric arterial flow (, ultrasonic flow probe) were then performed before and after NG-nitro-L-arginine methyl ester (L-NAME) injection (13 mg/kg intravenously). RESULTS:Short-term or long-term indomethacin treatment had no effect in sham-operated rats but significantly decreased mesenteric arterial flow in portal-hypertensive rats. Mesenteric flow remained higher after long-term than after short-term indomethacin treatment (54.5 +/- 2 vs. 46.1 +/- 2; P = 0.01). This blunted response to long-term indomethacin treatment was associated with an enhanced response to L-NAME, shown by greater increments in arterial pressure (29% +/- 3%) and mesenteric arterial resistance (209 +/- 22%) in indomethacin-treated rats than in rats receiving vehicle (19% +/- 2% and 130% +/- 20%; P < 0.05). CONCLUSIONS:Both prostacyclin and NO contributed to splanchnic hyperemia in portal-hypertensive rats. There was an enhanced release of NO after long-term prostacyclin inhibition, suggesting that both vasodilatory systems interact, promoting splanchnic hyperemia in portal hypertension.






Fernández M,García-Pagán JC,Casadevall M,Mourelle MI,Piqué JM,Bosch J,Rodés J




Has Abstract


1996-05-01 00:00:00














  • Amitriptyline-induced prolonged cholestasis.

    abstract::We report the case of a patient in whom amitriptyline administration for 5 wk was followed by prolonged cholestasis. Jaundice and pruritus lasted 19 and 20 mo, respectively. Three liver biopsies were performed at different stages of the disease showing the course of liver lesions. Cholestasis initially located in the ...


    pub_type: 杂志文章


    authors: Larrey D,Amouyal G,Pessayre D,Degott C,Danne O,Machayekhi JP,Feldmann G,Benhamou JP

    更新日期:1988-01-01 00:00:00

  • Caerulein-induced acute necrotizing pancreatitis in mice: protective effects of proglumide, benzotript, and secretin.

    abstract::The onset, course, and regression of the biochemical and structural alterations associated with pancreatitis induced by various doses of caerulein were studied in the mouse. In addition, the protective effect of secretin was compared with that of the cholecystokinin-receptor antagonists proglumide and benzotript. Subc...


    pub_type: 杂志文章


    authors: Niederau C,Ferrell LD,Grendell JH

    更新日期:1985-05-01 00:00:00

  • A comparative analysis by SAGE of gene expression profiles of Barrett's esophagus, normal squamous esophagus, and gastric cardia.

    abstract:BACKGROUND & AIMS:The metaplastic process in which the normal squamous epithelium of the distal esophagus is replaced by columnar-lined epithelium, known as Barrett's esophagus (BE), is poorly understood. The aim of this study was to define, analyze, and compare transcription profiles of BE, normal cardia epithelium, a...


    pub_type: 杂志文章


    authors: van Baal JW,Milano F,Rygiel AM,Bergman JJ,Rosmolen WD,van Deventer SJ,Wang KK,Peppelenbosch MP,Krishnadath KK

    更新日期:2005-10-01 00:00:00

  • Involvement of eicosanoids and macrophage-like cells in cytokine-mediated changes in rat myenteric nerves.

    abstract:BACKGROUND & AIMS:Proinflammatory cytokines alter function in enteric nerves, but little is known about underlying mechanisms. This study was designed to investigate the roles of prostanoids and of macrophage-like cells in cytokine-induced suppression of [3H]norepinephrine release from rat myenteric plexus. METHODS:Th...


    pub_type: 杂志文章


    authors: Rühl A,Berezin I,Collins SM

    更新日期:1995-12-01 00:00:00

  • Combined targeted treatment to eliminate tumorigenic cancer stem cells in human pancreatic cancer.

    abstract:BACKGROUND & AIMS:Pancreatic cancers contain exclusively tumorigenic cancer stem cells (CSCs), which are highly resistant to chemotherapy, resulting in a relative increase in CSC numbers during gemcitabine treatment. Signaling through sonic hedgehog and mammalian target of rapamycin (mTOR), respectively, may be essenti...


    pub_type: 杂志文章


    authors: Mueller MT,Hermann PC,Witthauer J,Rubio-Viqueira B,Leicht SF,Huber S,Ellwart JW,Mustafa M,Bartenstein P,D'Haese JG,Schoenberg MH,Berger F,Jauch KW,Hidalgo M,Heeschen C

    更新日期:2009-09-01 00:00:00

  • LST-2, a human liver-specific organic anion transporter, determines methotrexate sensitivity in gastrointestinal cancers.

    abstract:BACKGROUND & AIMS:One approach to the development of targeted cancer chemotherapy exploits increased uptake of the agent into neoplastic cells. In this scenario, higher concentrations of the agent in cancer cells are responsible for differential killing, whereas the low concentration in normal human cells decreases sid...


    pub_type: 杂志文章


    authors: Abe T,Unno M,Onogawa T,Tokui T,Kondo TN,Nakagomi R,Adachi H,Fujiwara K,Okabe M,Suzuki T,Nunoki K,Sato E,Kakyo M,Nishio T,Sugita J,Asano N,Tanemoto M,Seki M,Date F,Ono K,Kondo Y,Shiiba K,Suzuki M,Ohtani H,S

    更新日期:2001-06-01 00:00:00

  • Orthotopic liver transplantation for graft-versus-host disease following bone marrow transplantation.

    abstract::Chronic graft-vs.-host disease occurs in 30%-50% of long-term survivors of allogeneic bone marrow grafts, and may eventuate in cirrhosis. In this study, a young woman, originally diagnosed as having acute myelogenous leukemia, underwent successful bone marrow transplantation but later developed graft-vs.-host disease-...


    pub_type: 杂志文章


    authors: Rhodes DF,Lee WM,Wingard JR,Pavy MD,Santos GW,Shaw BW,Wood RP,Sorrell MF,Markin RS

    更新日期:1990-08-01 00:00:00

  • Oral contraceptive use and smoking are risk factors for relapse in Crohn's disease. The Canadian Mesalamine for Remission of Crohn's Disease Study Group.

    abstract:BACKGROUND & AIMS:Lifestyle factors have been shown to influence prognosis in Crohn's disease. The purpose of this study was to prospectively assess the effects of smoking and oral contraceptive use on clinical relapse rates. METHODS:Placebo-treated patients formed a prospective cohort, followed up for 48 weeks or unt...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Timmer A,Sutherland LR,Martin F

    更新日期:1998-06-01 00:00:00

  • NLRP3 Inflammasome Regulates Development of Systemic Inflammatory Response and Compensatory Anti-Inflammatory Response Syndromes in Mice With Acute Pancreatitis.

    abstract:BACKGROUND & AIMS:Pancreatitis starts with primarily sterile local inflammation that induces systemic inflammatory response syndrome, followed by compensatory anti-inflammatory response syndrome (CARS). We investigated the mechanisms of these processes in mice and human serum. METHODS:We induced severe acute pancreati...


    pub_type: 杂志文章


    authors: Sendler M,van den Brandt C,Glaubitz J,Wilden A,Golchert J,Weiss FU,Homuth G,De Freitas Chama LL,Mishra N,Mahajan UM,Bossaller L,Völker U,Bröker BM,Mayerle J,Lerch MM

    更新日期:2020-01-01 00:00:00

  • Esomeprazole with clopidogrel reduces peptic ulcer recurrence, compared with clopidogrel alone, in patients with atherosclerosis.

    abstract:BACKGROUND & AIMS:We performed a prospective, randomized, controlled study to compare the combination of esomeprazole and clopidogrel vs clopidogrel alone in preventing recurrent peptic ulcers in patients with atherosclerosis and a history of peptic ulcers. We also investigated the effects of esomeprazole on the antipl...


    pub_type: 杂志文章,随机对照试验


    authors: Hsu PI,Lai KH,Liu CP

    更新日期:2011-03-01 00:00:00

  • Intestinal villus and crypt cell responses to cholera toxin.

    abstract::Adenylate cyclase activity was measured in rat small intestinal villus and crypt cells after in vivo and in vitro exposure to cholera toxin. The increase in intestinal adenylate cyclase induced by cholera toxin in vivo appeared to be largely confined to the villus cell with the largest increase observed for upper vill...


    pub_type: 杂志文章


    authors: Weiser MM,Quill H

    更新日期:1975-08-01 00:00:00

  • Natural killer activity of human lymphocytes against colon cancer cells.

    abstract::A human colon cancer cell line, HCT-8, is shown to be an appropriate target cell for study of natural killer (NK) activity in man. In parallel experiments, effector cell characteristics for NK and antibody-dependent cellular cytotoxicity (ADCC) were found to be vested in a single lymphocyte subpopulation, which bore r...


    pub_type: 杂志文章


    authors: Helms RA,Bull DM

    更新日期:1980-04-01 00:00:00

  • The stimulation of albumin sythesis by methadone.

    abstract::Elevated levels of serum albumin have been noted in patients on chronic methadone maintenance and in heroin addicts. This observation was investigated in rabbits maintained on daily methadone 4 mg per kg of body weight after a period of 3 months on increasing dosage to assure drug tolerance. Albumin distribution and m...


    pub_type: 杂志文章


    authors: Rothschild MA,Kreek MJ,Oratz M,Schreiber SS,Mongelli JG

    更新日期:1976-08-01 00:00:00

  • The homeodomain protein Cdx2 regulates lactase gene promoter activity during enterocyte differentiation.

    abstract:BACKGROUND & AIMS:Lactase is the intestinal disaccharidase responsible for digestion of lactose, the predominant carbohydrate in milk. Transcription of the lactase gene is activated during enterocyte differentiation. We have characterized the interaction between the lactase promoter and Cdx2, a homeodomain protein invo...


    pub_type: 杂志文章


    authors: Fang R,Santiago NA,Olds LC,Sibley E

    更新日期:2000-01-01 00:00:00

  • h-sgk serine-threonine protein kinase gene as transcriptional target of transforming growth factor beta in human intestine.

    abstract:BACKGROUND & AIMS:Recently, the immediate early gene h-sgk was cloned as a hypertonicity-induced gene from human hepatoma cells. The aim of this study was to localize h-sgk messenger RNA (mRNA) expression in normal and inflamed intestinal mucosa and to identify potential transcriptional regulators. METHODS:h-sgk mRNA ...


    pub_type: 杂志文章


    authors: Waldegger S,Klingel K,Barth P,Sauter M,Rfer ML,Kandolf R,Lang F

    更新日期:1999-05-01 00:00:00

  • Caloric intake and unconjugated hyperbilirubinemia.

    abstract::Reduction in caloric intake was associated with a greater absolute rise in the serum bilirubin concentration in patients with Gilbert's syndrome and partial hepatic bilirubin uridine diphosphate glucuronyltransferase (UDPG-T) dysfunction compared to patients with hemolytic unconjugated hyperbilirubinemia and normal su...


    pub_type: 杂志文章


    authors: Felsher BF,Carpio NM

    更新日期:1975-07-01 00:00:00

  • Reactive nitrogen species switch on early extracellular matrix remodeling via induction of MMP1 and TNFalpha.

    abstract:BACKGROUND & AIMS:Liver injury leads to generation of reactive oxygen and nitrogen species, which can react to produce peroxynitrite (ONOO-). We investigated whether ONOO- and its metabolites modulate extracellular matrix remodeling. METHODS:Stellate cells (HSC) were incubated with pure ONOO- or SIN-1 (a ONOO- donor)....


    pub_type: 杂志文章


    authors: Urtasun R,Cubero FJ,Vera M,Nieto N

    更新日期:2009-04-01 00:00:00

  • CD39/ENTPD1 expression by CD4+Foxp3+ regulatory T cells promotes hepatic metastatic tumor growth in mice.

    abstract:BACKGROUND & AIMS:Adenosine mediates immune suppression and is generated by the ectonucleotidases CD39 (ENTPD1) and CD73 that are expressed on vascular endothelial cells and regulatory T cells (Tregs). Although tumor-infiltrating immune cells include Foxp3(+) Tregs, it is not clear whether local adenosine generation by...


    pub_type: 杂志文章


    authors: Sun X,Wu Y,Gao W,Enjyoji K,Csizmadia E,Müller CE,Murakami T,Robson SC

    更新日期:2010-09-01 00:00:00

  • Activation of mast cells by bile acids.

    abstract::To test whether bile acids interact with mast cells, dilute, aqueous solutions of five pure unconjugated natural bile acids and their corresponding glycine or taurine conjugates were incubated with murine PT-18 cells (a mast cell line functionally and cytochemically similar to mucosal mast cells) or with freshly isola...


    pub_type: 杂志文章


    authors: Quist RG,Ton-Nu HT,Lillienau J,Hofmann AF,Barrett KE

    更新日期:1991-08-01 00:00:00

  • Helicobacter pylori, inflammation, mucosal damage, and apoptosis: pathogenesis and definition of gastric atrophy.

    abstract::Much of what is currently accepted on the natural history of Helicobacter pylori-induced gastritis and its relationship with gastric adenocarcinoma rests on the assumption that atrophic gastritis can be correctly identified and reproducibly recognized. Recently, several studies have indicated that pathologists have a ...


    pub_type: 杂志文章,评审


    authors: Genta RM

    更新日期:1997-12-01 00:00:00

  • MLH1 germline epimutations as a factor in hereditary nonpolyposis colorectal cancer.

    abstract:BACKGROUND & AIMS:Hereditary nonpolyposis colorectal cancer (HNPCC) is caused by heterozygous germline sequence mutations of DNA mismatch repair genes, most frequently MLH1 or MSH2. A novel molecular mechanism for HNPCC has recently been suggested by the finding of individuals with soma-wide monoallelic hypermethylatio...


    pub_type: 杂志文章


    authors: Hitchins M,Williams R,Cheong K,Halani N,Lin VA,Packham D,Ku S,Buckle A,Hawkins N,Burn J,Gallinger S,Goldblatt J,Kirk J,Tomlinson I,Scott R,Spigelman A,Suter C,Martin D,Suthers G,Ward R

    更新日期:2005-11-01 00:00:00

  • Patients with colorectal tumors with microsatellite instability and large deletions in HSP110 T17 have improved response to 5-fluorouracil–based chemotherapy.

    abstract:BACKGROUND & AIMS:Patients with colorectal tumors with microsatellite instability (MSI) have better prognoses than patients with tumors without MSI, but have a poor response to 5-fluorouracil–based chemotherapy. A dominant-negative form of heat shock protein (HSP)110 (HSP110DE9) expressed by cancer cells with MSI, via ...


    pub_type: 杂志文章,多中心研究


    authors: Collura A,Lagrange A,Svrcek M,Marisa L,Buhard O,Guilloux A,Wanherdrick K,Dorard C,Taieb A,Saget A,Loh M,Soong R,Zeps N,Platell C,Mews A,Iacopetta B,De Thonel A,Seigneuric R,Marcion G,Chapusot C,Lepage C,Bouvier

    更新日期:2014-02-01 00:00:00

  • Safety of Proton Pump Inhibitors Based on a Large, Multi-Year, Randomized Trial of Patients Receiving Rivaroxaban or Aspirin.

    abstract:BACKGROUND & AIMS:Proton pump inhibitors (PPIs) are effective at treating acid-related disorders. These drugs are well tolerated in the short term, but long-term treatment was associated with adverse events in observational studies. We aimed to confirm these findings in an adequately powered randomized trial. METHODS:...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Moayyedi P,Eikelboom JW,Bosch J,Connolly SJ,Dyal L,Shestakovska O,Leong D,Anand SS,Störk S,Branch KRH,Bhatt DL,Verhamme PB,O'Donnell M,Maggioni AP,Lonn EM,Piegas LS,Ertl G,Keltai M,Bruns NC,Muehlhofer E,Dagenais G

    更新日期:2019-09-01 00:00:00

  • Peptidergic and nitrinergic denervation in congenital esophageal stenosis.

    abstract::Congenital esophageal stenosis (CES) is a rare disorder with narrowed esophageal lumen that presents as dysphagia from childhood and that is often associated with tracheobronchial remnants or webs. The pathogenesis of CES is unknown. The aim of this study was to examine the histological and immunohistochemical feature...


    pub_type: 杂志文章


    authors: Singaram C,Sweet MA,Gaumnitz EA,Cameron AJ,Camilleri M

    更新日期:1995-07-01 00:00:00

  • Lamivudine therapy for chronic hepatitis B: a six-month randomized dose-ranging study.

    abstract:BACKGROUND & AIMS:Lamivudine inhibits hepatitis B virus replication. This study investigated 6 months of lamivudine treatment at three doses. METHODS:Fifty-one patients (43% white, 49% Asian) with chronic hepatitis B were randomly assigned to receive 25, 100, or 300 mg of lamivudine orally once daily for 24 weeks with...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Nevens F,Main J,Honkoop P,Tyrrell DL,Barber J,Sullivan MT,Fevery J,De Man RA,Thomas HC

    更新日期:1997-10-01 00:00:00

  • The carbohydrate composition of mucin in colonic cancer.

    abstract::Mucins synthesized in colonic cancer are known to be different from those in the normal colon; however, the biochemical differences between these mucins have not been defined. We have purified mucins from samples of nonneoplastic (normal) human colon and colon cancer and found that the carbohydrate content of the canc...


    pub_type: 杂志文章


    authors: Boland CR,Deshmukh GD

    更新日期:1990-05-01 00:00:00

  • Distinct temporal-spatial roles for rho kinase and myosin light chain kinase in epithelial purse-string wound closure.

    abstract:BACKGROUND & AIMS:Small epithelial wounds heal by purse-string contraction of an actomyosin ring that is regulated by myosin light chain (MLC) kinase (MLCK) and rho kinase (ROCK). These studies aimed to define the roles of these kinases in purse-string wound closure. METHODS:Oligocellular and single-cell wounds were c...


    pub_type: 杂志文章


    authors: Russo JM,Florian P,Shen L,Graham WV,Tretiakova MS,Gitter AH,Mrsny RJ,Turner JR

    更新日期:2005-04-01 00:00:00

  • Somatosensory evoked potentials in hepatic encephalopathy.

    abstract::Median nerve somatosensory evoked potentials were recorded from 33 patients with various degrees of hepatic failure and from 10 age-matched controls. Within 20 ms poststimulation, one negative peak (N13) could be recorded from the middle of the back of the patient's neck at the C2 vertebral level. Within 150 ms, three...


    pub_type: 杂志文章


    authors: Yang SS,Chu NS,Liaw YF

    更新日期:1985-09-01 00:00:00

  • Effect of recombinant platelet-activating factor acetylhydrolase on two models of experimental acute pancreatitis.

    abstract:BACKGROUND & AIMS:Recent reports suggest that platelet-activating factor (PAF) plays a role in pancreatitis and pancreatitis-associated lung injury. In this study, the effects on these processes of termination of PAF action by recombinant PAF-acetylhydrolase (rPAF-AH) were investigated. METHODS:Rats were given rPAF-AH...


    pub_type: 杂志文章


    authors: Hofbauer B,Saluja AK,Bhatia M,Frossard JL,Lee HS,Bhagat L,Steer ML

    更新日期:1998-11-01 00:00:00

  • The Apc 1322T mouse develops severe polyposis associated with submaximal nuclear beta-catenin expression.

    abstract:BACKGROUND & AIMS:We previously demonstrated that the 2 APC mutations in human colorectal tumors are coselected, because tumorigenesis requires an optimal level of Wnt signaling. We and others subsequently showed that the truncated APC proteins in colorectal tumors usually retain a total of 1-2 beta-catenin binding/deg...


    pub_type: 杂志文章


    authors: Pollard P,Deheragoda M,Segditsas S,Lewis A,Rowan A,Howarth K,Willis L,Nye E,McCart A,Mandir N,Silver A,Goodlad R,Stamp G,Cockman M,East P,Spencer-Dene B,Poulsom R,Wright N,Tomlinson I

    更新日期:2009-06-01 00:00:00