Definition of the initial immunologic modifications upon in vitro gliadin challenge in the small intestine of celiac patients.


BACKGROUND & AIMS:Mucosal cell-mediated immune response is considered the central event in the pathogenesis of celiac disease. In cultured intestinal explants from celiacs in remission, we have characterized the early stages of gliadin-induced immune activation. METHODS:Intestinal biopsy specimens (15 treated celiacs and 13 controls) were cultured with gliadin or maize prolamine digests for 24 hours as well as for 1, 2, 4, 6, 8, and 12 hours in some subjects. The expression of immunologic markers was detected by immunocytochemistry. RESULTS:Gliadin challenge may initiate two parallel pathways, one of which leads to T-cell activation and another that precedes it. Epithelial cells overexpress DR molecules after 1 hour, and in a second stage T lymphocytes become fully activated. Moreover, T lymphocytes migrate in the upper mucosal layers. T lymphocytes that migrate in the higher lamina propria compartments are mainly CD4+ and show markers of activation; migrating intraepithelial lymphocytes are CD8+ and do not express these markers. CONCLUSIONS:In vitro gliadin challenge is a suitable model to reproduce various immunologic features of celiac lesions; these may be caused by different pathways. The comprehension of these phenomena is essential to clarify the distinctive pathogenic mechanisms leading to disease and may help in defining novel therapeutic approaches.






Maiuri L,Picarelli A,Boirivant M,Coletta S,Mazzilli MC,De Vincenzi M,Londei M,Auricchio S




Has Abstract


1996-05-01 00:00:00














  • Comparison of endogenous and radiolabeled bile acid excretion in patients with idiopathic chronic diarrhea.

    abstract::Fecal recovery of radioactivity after ingestion of a bolus of radiolabeled bile acid is abnormally high in most patients with idiopathic chronic diarrhea. To evaluate the significance of this malabsorption, concurrent fecal excretion of both exogenous radiolabeled bile acid and endogenous (unlabeled) bile acid were me...


    pub_type: 杂志文章


    authors: Schiller LR,Bilhartz LE,Santa Ana CA,Fordtran JS

    更新日期:1990-04-01 00:00:00

  • Effect of cell culture on rabbit colonic smooth muscle bradykinin receptors.

    abstract::The aim of this study was to assess the effect of cell culture on the bradykinin receptor of rabbit colon myocytes. In longitudinal muscle strips prepared from distal colon, bradykinin stimulated dose-dependent contraction that was 62% of the maximal response to bethanecol. At 4 degrees C, [3H]bradykinin binding to fr...


    pub_type: 杂志文章


    authors: Hyman PE,Diego A,Ridout D,Yuan QX,Ennes H,Cominelli F,Snape WJ Jr,Tomomasa T

    更新日期:1992-05-01 00:00:00

  • A novel enzyme immunoassay for serodiagnosis of Helicobacter pylori infection.

    abstract::Helicobacter pylori has recently been implicated as an etiologic agent of gastroduodenal disorders. Comparing the antibody to H. pylori in the sera of patients with that of normal controls by Western blot analysis, a unique antibody was detected in the sera of patients, which reacted with the 25-kilodalton antigen of ...


    pub_type: 杂志文章


    authors: Sugiyama T,Imai K,Yoshida H,Takayama Y,Yabana T,Yokota K,Oguma K,Yachi A

    更新日期:1991-07-01 00:00:00

  • Initial endoscopy or empirical therapy with or without testing for Helicobacter pylori for dyspepsia: a decision analysis.

    abstract:BACKGROUND & AIMS:Empirical therapy has been proposed for initial management of dyspepsia. The aim of this study was to evaluate initial endoscopy, empirical therapy, and testing for Helicobacter pylori in the management of patients with a new onset of dyspepsia. METHODS:Decision analysis was used to compare the direc...


    pub_type: 杂志文章


    authors: Silverstein MD,Petterson T,Talley NJ

    更新日期:1996-01-01 00:00:00

  • Active Choice and Financial Incentives to Increase Rates of Screening Colonoscopy-A Randomized Controlled Trial.

    abstract::Behavioral economic approaches could increase uptake for colorectal cancer screening. We performed a randomized controlled trial of 2245 employees to determine whether an email containing a phone number for scheduling (control), an email with the active choice to opt in or opt out (active choice), or the active choice...


    pub_type: 杂志文章,随机对照试验


    authors: Mehta SJ,Feingold J,Vandertuyn M,Niewood T,Cox C,Doubeni CA,Volpp KG,Asch DA

    更新日期:2017-11-01 00:00:00

  • Quantitative fecal indium 111-labeled leukocyte excretion in the assessment of disease in Crohn's disease.

    abstract::The assessment of disease activity in Crohn's disease involves determination of either clinical indexes (e.g., Crohn's disease activity index) or laboratory measurements (e.g., C-reactive protein and erythrocyte sedimentation rate). These have the disadvantage of being indirect and nonspecific correlates of gut inflam...


    pub_type: 杂志文章


    authors: Saverymuttu SH,Peters AM,Lavender JP,Pepys MB,Hodgson HJ,Chadwick VS

    更新日期:1983-12-01 00:00:00

  • Inflammatory modulation of calcium-activated potassium channels in canine colonic circular smooth muscle cells.

    abstract:BACKGROUND & AIMS:The characteristics of colonic circular smooth muscle slow waves are altered during inflammation. The aim of this study was to examine whether inflammation modulates the open-state probability of Ca2+-activated K+ (KCa) channels in these cells to contribute to these alterations. METHODS:The experimen...


    pub_type: 杂志文章


    authors: Lu G,Mazet B,Sun C,Qian X,Johnson CP,Adams MB,Roman RJ,Sarna SK

    更新日期:1999-04-01 00:00:00

  • Inflammation and disruption of the mucosal architecture in claudin-7-deficient mice.

    abstract:BACKGROUND & AIMS:Integrity of the intestinal epithelium is required for nutrition absorption and defense against pathogens. Claudins are cell adhesion molecules that localize at tight junctions (TJs); many are expressed in the intestinal tract, but little is known about their functions. Claudin-7 is unique in that it ...


    pub_type: 杂志文章


    authors: Ding L,Lu Z,Foreman O,Tatum R,Lu Q,Renegar R,Cao J,Chen YH

    更新日期:2012-02-01 00:00:00

  • Quantification of epigenetic and genetic 2nd hits in CDH1 during hereditary diffuse gastric cancer syndrome progression.

    abstract:BACKGROUND & AIMS:Hereditary diffuse gastric cancer (HDGC) families carry CDH1 heterozygous germline mutations; their tumors acquire complete CDH1 inactivation through "2nd-hit" mechanisms. Most frequently, this occurs via promoter hypermethylation (epigenetic modification), and less frequently via CDH1 mutations and l...


    pub_type: 杂志文章


    authors: Oliveira C,Sousa S,Pinheiro H,Karam R,Bordeira-Carriço R,Senz J,Kaurah P,Carvalho J,Pereira R,Gusmão L,Wen X,Cipriano MA,Yokota J,Carneiro F,Huntsman D,Seruca R

    更新日期:2009-06-01 00:00:00

  • Drug- and estrogen-induced cholestasis through inhibition of the hepatocellular bile salt export pump (Bsep) of rat liver.

    abstract:BACKGROUND & AIMS:Drug-induced cholestasis is a frequent form of acquired liver disease. To elucidate the molecular pathogenesis of drug-induced cholestasis, we investigated the effects of prototypic cholestatic drugs on the canalicular bile salt export pump (Bsep) of rat liver. METHODS:Vesicles were isolated from Bse...


    pub_type: 杂志文章


    authors: Stieger B,Fattinger K,Madon J,Kullak-Ublick GA,Meier PJ

    更新日期:2000-02-01 00:00:00

  • Endoscopic Submucosal Dissection: Indications and Application in Western Endoscopy Practice.

    abstract::Endoscopic submucosal dissection was developed in Japan, early in this century, to provide a minimally invasive yet curative treatment for the large numbers of patients with early gastric cancer identified by the national screening program. Previously, the majority of these patients were treated surgically at substant...


    pub_type: 杂志文章,评审


    authors: Bourke MJ,Neuhaus H,Bergman JJ

    更新日期:2018-05-01 00:00:00

  • Identification of Prognostic Phenotypes of Esophageal Adenocarcinoma in 2 Independent Cohorts.

    abstract:BACKGROUND & AIMS:Most patients with esophageal adenocarcinoma (EAC) present with de novo tumors. Although this could be due to inadequate screening strategies, the precise reason for this observation is not clear. We compared survival of patients with prevalent EAC with and without synchronous Barrett esophagus (BE) w...


    pub_type: 杂志文章,多中心研究


    authors: Sawas T,Killcoyne S,Iyer PG,Wang KK,Smyrk TC,Kisiel JB,Qin Y,Ahlquist DA,Rustgi AK,Costa RJ,Gerstung M,Fitzgerald RC,Katzka DA,OCCAMS Consortium.

    更新日期:2018-12-01 00:00:00

  • Selective blockade of DCAMKL-1 results in tumor growth arrest by a Let-7a MicroRNA-dependent mechanism.

    abstract:BACKGROUND & AIMS:MicroRNAs (miRNAs) are non-coding RNAs that regulate gene expression. The tumor suppressor miRNA let-7a has been reported to be inhibited posttranscriptionally in embryonic stem cells and in human cancers. Microtubule-associated kinase DCAMKL-1 is a putative intestinal stem cell marker that is express...


    pub_type: 杂志文章


    authors: Sureban SM,May R,Ramalingam S,Subramaniam D,Natarajan G,Anant S,Houchen CW

    更新日期:2009-08-01 00:00:00

  • Emergence of antigenic glycoprotein structures in ulcerative colitis detected through monoclonal antibodies.

    abstract::The emergence of new glycoprotein structures in colonic mucosa from patients with ulcerative colitis (UC) was assessed through the development of monoclonal antibodies (MAbs). Hybridomas were prepared from mice immunized with mucin glycoproteins purified from UC colonic tissue. Supernatants of 11 fusion products among...


    pub_type: 杂志文章


    authors: Podolsky DK,Fournier DA

    更新日期:1988-08-01 00:00:00

  • Genetic variants in cyclooxygenase-2: Expression and risk of gastric cancer and its precursors in a Chinese population.

    abstract:BACKGROUND & AIMS:To screen the genetic variants in cyclooxygenase-2 (COX-2), and evaluate their effects on COX-2 expression and risk of gastric cancer (GC) and its precursors, a population-based study was conducted in Linqu County, Shangdong Province, an area of China, a high-risk area of GC. METHODS:Genotypes were d...


    pub_type: 杂志文章


    authors: Liu F,Pan K,Zhang X,Zhang Y,Zhang L,Ma J,Dong C,Shen L,Li J,Deng D,Lin D,You W

    更新日期:2006-06-01 00:00:00

  • Influence of vasopressin on colon blood flow in monkeys.

    abstract::Inferior mesenteric arterial blood flow was measured with an electromagnetic blood flowmeter in five anesthetized rhesus monkeys. The effects of vasopressin on this vasculature were determined to evaluate the optimal, safe concentration of this agent during its clinical application in the management of hemorrhagic les...


    pub_type: 杂志文章


    authors: Kerr JC,Hobson RW 2nd,Seelig RF,Swan KG

    更新日期:1977-03-01 00:00:00

  • Primary prophylaxis of variceal hemorrhage: a randomized controlled trial comparing band ligation, propranolol, and isosorbide mononitrate.

    abstract:BACKGROUND & AIMS:This randomized controlled trial compared variceal band ligation (VBL), propranolol (PPL), and isosorbide-5-mononitrate (ISMN) in the prevention of first esophageal variceal bleed. METHODS:Over a 6-year period, 172 patients with cirrhosis, grade II or III esophageal varices that had never bled, were ...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Lui HF,Stanley AJ,Forrest EH,Jalan R,Hislop WS,Mills PR,Finlayson ND,Macgilchrist AJ,Hayes PC

    更新日期:2002-09-01 00:00:00

  • Double-blind comparison of slow-release 5-aminosalicylate and sulfasalazine in remission maintenance in ulcerative colitis.

    abstract::The results of a clinical trial comparing slow-release 5-aminosalicylic acid tablets (Pentasa) and enteric-coated sulfasalazine tablets (Salazopyrin) with regard to the efficacy of maintaining ulcerative colitis patients in remission for 12 mo and with regard to safety of treatment are reported. Seventy-five patients ...


    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验


    authors: Mulder CJ,Tytgat GN,Weterman IT,Dekker W,Blok P,Schrijver M,van der Heide H

    更新日期:1988-12-01 00:00:00

  • Neurokinin 1 and 2 receptors mediate cholera toxin secretion in rat jejunum.

    abstract:BACKGROUND & AIMS:Substance P, a member of the tachykinin family, is a prosecretory neuropeptide distributed widely throughout the enteric nervous system. Implicated in inflammatory states, its role in enterotoxigenic water and electrolyte secretion is unclear. We assessed the effect of substance P antagonists and neur...


    pub_type: 杂志文章


    authors: Turvill JL,Connor P,Farthing MJ

    更新日期:2000-10-01 00:00:00

  • Bile-salt metabolism in the primate fetus.

    abstract::Fetal bile salt metabolism was assessed by the intravenous infusion of e114C]cholate into 6 monkey fetuses. The placental transfer, fetal plasma clearance, and fetal hepatic excretion of the administered radiolabeled bile salt were measured. Placental transfer averaged 30% of the injected dose, while biliary excretion...


    pub_type: 杂志文章


    authors: Little JM,Smallwood RA,Lester R,Piasecki GJ,Jackson BT

    更新日期:1975-12-01 00:00:00

  • Hepatic artery embolization for metastatic endocrine-secreting tumors of the pancreas. Report of two cases.

    abstract::Two patients with hormone-secreting metastatic tumors of the liver (carcinoid, islet cell) were treated with transcatheter arterial embolization. In both cases, occlusion of blood supply to the tumors was accomplished, resulting in cessation of the systemic symptoms. Arterial embolization provides another useful palli...


    pub_type: 杂志文章


    authors: Clouse ME,Lee RG,Duszlak EJ,Lokich JJ,Alday MT

    更新日期:1983-11-01 00:00:00

  • Communication via gap junctions modulates bile secretion in the isolated perfused rat liver.

    abstract:BACKGROUND & AIMS:Bile secretion is regulated in part by adenosine 3',5'-cyclic monophosphate (cAMP) and cytosolic Ca2+ (Ca2+i). Hormone receptors that link to these second messengers are not uniformly distributed across the hepatic lobule, but both cAMP and Ca2+i cross gap junctions, so we tested whether gap junctiona...


    pub_type: 杂志文章


    authors: Nathanson MH,Rios-Velez L,Burgstahler AD,Mennone A

    更新日期:1999-05-01 00:00:00

  • Administration of secretin (RG1068) increases the sensitivity of detection of duct abnormalities by magnetic resonance cholangiopancreatography in patients with pancreatitis.

    abstract:BACKGROUND & AIMS:Administration of secretin improves noninvasive imaging of the pancreatic duct with magnetic resonance cholangiopancreatography (MRCP). We performed a large prospective study to investigate whether synthetic human secretin (RG1068)-stimulated MRCP detects pancreatic duct abnormalities with higher leve...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Sherman S,Freeman ML,Tarnasky PR,Wilcox CM,Kulkarni A,Aisen AM,Jacoby D,Kozarek RA

    更新日期:2014-09-01 00:00:00

  • Toll-like receptor 2 regulates intestinal inflammation by controlling integrity of the enteric nervous system.

    abstract:BACKGROUND & AIMS:In the intestines, Toll-like receptor 2 (TLR2) mediates immune responses to pathogens and regulates epithelial barrier function; polymorphisms in TLR2 have been associated with inflammatory bowel disease phenotype. We assessed the effects of TLR2 signaling on the enteric nervous system (ENS) in mice. ...


    pub_type: 杂志文章


    authors: Brun P,Giron MC,Qesari M,Porzionato A,Caputi V,Zoppellaro C,Banzato S,Grillo AR,Spagnol L,De Caro R,Pizzuti D,Barbieri V,Rosato A,Sturniolo GC,Martines D,Zaninotto G,Palù G,Castagliuolo I

    更新日期:2013-12-01 00:00:00

  • Glucagon-like peptide-2: update of the recent clinical trials.

    abstract::Although long-term parenteral nutrition is lifesaving in patients with intestinal failure, it is expensive and associated with serious complications such as catheter sepsis, venous occlusions, and liver failure and severely impairs the quality of life in the short bowel patients. Therefore, treatments that increase th...


    pub_type: 杂志文章,评审


    authors: Jeppesen PB

    更新日期:2006-02-01 00:00:00

  • Use of aspirin or nonsteroidal anti-inflammatory drugs increases risk for diverticulitis and diverticular bleeding.

    abstract:BACKGROUND & AIMS:Nonsteroidal anti-inflammatory drugs (NSAIDs), including aspirin, have been implicated in diverticular complications. We examined the influence of aspirin and NSAID use on risk of diverticulitis and diverticular bleeding in a large prospective cohort. METHODS:We studied 47,210 US men in the Health Pr...


    pub_type: 杂志文章


    authors: Strate LL,Liu YL,Huang ES,Giovannucci EL,Chan AT

    更新日期:2011-05-01 00:00:00

  • Nonsteroidal anti-inflammatory drugs and statins have chemopreventative effects in patients with Barrett's esophagus.

    abstract:BACKGROUND & AIMS:The incidence of Barrett's esophagus and esophageal adenocarcinoma has increased despite surveillance of patients with Barrett's esophagus. Limited data indicate that nonsteroidal anti-inflammatory drug (NSAID) and statin use reduce the risk for esophageal adenocarcinoma. We investigated whether NSAID...


    pub_type: 杂志文章,多中心研究


    authors: Kastelein F,Spaander MC,Biermann K,Steyerberg EW,Kuipers EJ,Bruno MJ,Probar-study Group.

    更新日期:2011-12-01 00:00:00

  • Smad4-mediated signaling inhibits intestinal neoplasia by inhibiting expression of β-catenin.

    abstract:BACKGROUND & AIMS:Mutational inactivation of adenomatous polyposis coli (APC) is an early event in colorectal cancer (CRC) progression that affects the stability and increases the activity of β-catenin, a mediator of Wnt signaling. Progression of CRC also involves inactivation of signaling via transforming growth facto...


    pub_type: 杂志文章


    authors: Freeman TJ,Smith JJ,Chen X,Washington MK,Roland JT,Means AL,Eschrich SA,Yeatman TJ,Deane NG,Beauchamp RD

    更新日期:2012-03-01 00:00:00

  • Therapy of hyponatremia in cirrhosis with a vasopressin receptor antagonist: a randomized double-blind multicenter trial.

    abstract:BACKGROUND & AIMS:Dilutional hyponatremia is a frequent complication of cirrhosis partly because of nonosmotic vasopressin release. No effective therapy exists for this complication. Therefore, we investigated the effects of VPA-985, an orally active vasopressin V2 receptor antagonist, in patients with cirrhosis and di...


    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验


    authors: Gerbes AL,Gülberg V,Ginès P,Decaux G,Gross P,Gandjini H,Djian J,VPA Study Group.

    更新日期:2003-04-01 00:00:00

  • Pancreatic acinar cell function and morphology in rats fed zinc-deficient and marginal zinc-deficient diets.

    abstract::The prevalence of marginal zinc nutriture in several populations of people in this country and the lack of reports on the effect of marginal zinc nutriture in experimental animals prompted us to look at pancreatic acinar cell function and morphology in rats fed a zinc-deficient diet ad libitum: 4 and 50 ppm zinc-suppl...


    pub_type: 杂志文章


    authors: Perez-Jimenez F,Bockman DE,Singh M

    更新日期:1986-04-01 00:00:00