Abstract：
BACKGROUND & AIMS:Mucosal cell-mediated immune response is considered the central event in the pathogenesis of celiac disease. In cultured intestinal explants from celiacs in remission, we have characterized the early stages of gliadin-induced immune activation. METHODS:Intestinal biopsy specimens (15 treated celiacs and 13 controls) were cultured with gliadin or maize prolamine digests for 24 hours as well as for 1, 2, 4, 6, 8, and 12 hours in some subjects. The expression of immunologic markers was detected by immunocytochemistry. RESULTS:Gliadin challenge may initiate two parallel pathways, one of which leads to T-cell activation and another that precedes it. Epithelial cells overexpress DR molecules after 1 hour, and in a second stage T lymphocytes become fully activated. Moreover, T lymphocytes migrate in the upper mucosal layers. T lymphocytes that migrate in the higher lamina propria compartments are mainly CD4+ and show markers of activation; migrating intraepithelial lymphocytes are CD8+ and do not express these markers. CONCLUSIONS:In vitro gliadin challenge is a suitable model to reproduce various immunologic features of celiac lesions; these may be caused by different pathways. The comprehension of these phenomena is essential to clarify the distinctive pathogenic mechanisms leading to disease and may help in defining novel therapeutic approaches.
journal_name
Gastroenterologyjournal_title
Gastroenterologyauthors
Maiuri L,Picarelli A,Boirivant M,Coletta S,Mazzilli MC,De Vincenzi M,Londei M,Auricchio Sdoi
10.1053/gast.1996.v110.pm8613040subject
Has Abstractpub_date
1996-05-01 00:00:00pages
1368-78issue
5eissn
0016-5085issn
1528-0012pii
S0016508596002065journal_volume
110pub_type
杂志文章相关文献
GASTROENTEROLOGY文献大全abstract：:The normal surface anatomy of rat hepatocytes, sinusoids, and bile canaliculi was examined by scanning electron microscopy following finger fracture of tissues that were perfused via the portal vein and fixed with 2.5% glutaraldehyde. These normal findings were contrasted with several models of cholestasis, including ...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1975-09-01 00:00:00
abstract：:Abdominal cramps and urgent defecation are common side effects of clinical doses of arginine vasopressin, indicating that the drug may have stimulating effects on colonic motor activity. Four strain-gauge transducers were implanted on the colon in six monkeys. A blood flow probe was fixed on the inferior mesenteric ar...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(92)90171-t
更新日期：1992-03-01 00:00:00
abstract：BACKGROUND & AIMS:Trinitrobenzene sulfonic acid (TNBS)-induced colitis is a T-helper 1 (Th1) T cell-mediated inflammation that is rapidly reversed by administration of anti-interleukin (IL) 12. This study sought to define the mechanism of this curative effect. METHODS:Cells and tissue from mice with TNBS colitis recei...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/s0016-5085(99)70392-6
更新日期：1999-11-01 00:00:00
abstract：:Evidence-based medicine combines clinical expertise and the best available evidence from systematic research to aid decision making in patient care. Levels of evidence can be graded from I to V, with level I, the strongest, coming from large randomized controlled trials (RCTs). When a definitive RCT has not been perfo...
journal_title：Gastroenterology
pub_type： 杂志文章,评审
doi：10.1016/s0016-5085(97)80022-4
更新日期：1997-12-01 00:00:00
abstract：:We studied the procoagulant activity of peripheral blood monocytes in 41 patients with severe obstructive jaundice and in 27 nonjaundiced control patients using a one-stage clotting assay. Mononuclear cells from jaundiced patients, tested immediately after isolation, expressed low levels of procoagulant activity, whic...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1989-03-01 00:00:00
abstract：:Most studies show that oral ingestion of iron does not cause a positive stool guaiac reaction. However, all in vitro studies show that iron does cause a positive guaiac reaction and some in vivo studies have shown a positive stool guaiac reaction in response to oral iron. This study examines this unresolved question. ...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(89)90766-x
更新日期：1989-01-01 00:00:00
abstract：BACKGROUND & AIMS:Hepatobiliary complications occur in inflammatory bowel disease and may be caused by the translocation of intestinal toxins from portal blood into bile through leaky hepatocyte tight junctions. The role of tight junctions in the pathogenesis of hepatobiliary complications in experimental inflammatory ...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/gast.1997.v113.pm9322530
更新日期：1997-10-01 00:00:00
abstract：:In vitro studies have shown that some bile salts inhibit the growth of intestinal bacteria. We investigated the possibility that bile salts might have a similar action in vivo in the ileum by performing serial anaerobic cultures and bile salt analyses on postprandial ileal fluid obtained anaerobically via intestinal t...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1975-08-01 00:00:00
abstract：:Topical 20% benzocaine (Hurricaine, Beutlich, Inc., Niles, Ill.) spray is frequently used for oral anesthesia before upper endoscopy. Side effects attributed to this agent are exceedingly rare. The author reports one of these rare complications, drug-induced methemoglobinemia, in a patient with methemoglobin reductase...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(90)91312-t
更新日期：1990-01-01 00:00:00
abstract：BACKGROUND/AIMS:The frequency of colorectal adenomas, the precursor lesions for most cases of colorectal carcinoma, has been generally measured as prevalence rates of adenomas at autopsy or colonoscopy. The aim of this study was to estimate the incidence rate of adenomas and compare it with the adenoma recurrence rate....
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(95)90066-7
更新日期：1995-02-01 00:00:00
abstract：:Although nocturnal acid secretion has been emphasized in the pathophysiology and treatment of duodenal ulcer, its importance in gastric ulcer disease has been questioned. To explore this area, this multicenter U.S. trial compared the effect of a once-daily nighttime dose of H2-receptor antagonist with placebo on the h...
journal_title：Gastroenterology
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1016/0016-5085(89)91489-3
更新日期：1989-10-01 00:00:00
abstract：BACKGROUND & AIMS:Hepatopulmonary syndrome (HPS) affects 10%-30% of patients with cirrhosis and portal hypertension and significantly increases mortality. Studies in experimental models indicate that pulmonary angiogenesis contributes to the development of HPS, but pathogenesis in humans is poorly understood. We invest...
journal_title：Gastroenterology
pub_type： 杂志文章,多中心研究
doi：10.1053/j.gastro.2010.03.044
更新日期：2010-07-01 00:00:00
abstract：BACKGROUND & AIMS:Numerous studies have linked the proliferation of liver progenitor cells (LPCs) during chronic liver disease to the risk for development of hepatocellular carcinoma. Thus, selective inhibition of LPC growth during preneoplastic injury may prevent or delay the onset of liver cancer. Rats carrying a ger...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2008.05.077
更新日期：2008-09-01 00:00:00
abstract：BACKGROUND & AIMS:Spontaneous hepatitis B surface antigen (HBsAg) seroclearance is a rare event in patients with chronic hepatitis B virus infection. The aim of this study was to clarify the controversy on long-term prognosis following spontaneous HBsAg seroclearance using a large series of patients. METHODS:A total o...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/gast.2002.36026
更新日期：2002-10-01 00:00:00
abstract：:The human lower esophageal sphincter (LES) is believed to be innervated by nonadrenergic, noncholinergic inhibitory nerves, and cholinergic excitatory nerves. In idiopathic achalasia, LES relaxation is abnormal because the inhibitory nerves to the sphincter are either absent or functionally impaired. The integrity of ...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(86)90869-3
更新日期：1986-04-01 00:00:00
abstract：BACKGROUND & AIMS:A high-fat diet (HFD) can cause serious health problems, including alteration of gastrointestinal transit, the exact mechanism of which is not clear. Several microRNAs (miRNAs) are involved in energy homeostasis, lipid metabolism, and HFD-induced weight gain. We investigated the role of miRNAs in HFD-...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2013.10.053
更新日期：2014-02-01 00:00:00
abstract：BACKGROUND & AIMS:Interactions between genetic and environmental factors are believed to be involved in onset and initiation of inflammatory bowel disease. We analyzed the interaction between gastrointestinal mucosal microbiota and host genes in twin pairs discordant for ulcerative colitis (UC) to study the functional ...
journal_title：Gastroenterology
pub_type： 杂志文章,多中心研究
doi：10.1053/j.gastro.2011.04.011
更新日期：2011-07-01 00:00:00
abstract：BACKGROUND & AIMS:ob/ob mice are used to study the mechanisms that regulate the progression from steatosis to nonalcoholic steatohepatitis. The livers of ob/ob mice are depleted of CD4-positive natural killer cells, components of the innate immune system that induce anti-inflammatory cytokines. Although this may explai...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/gast.2002.35997
更新日期：2002-10-01 00:00:00
abstract：BACKGROUND & AIMS:Screening colonoscopy is an effective method to reduce the incidence of and mortality from colorectal cancer (CRC). There is little empirical evidence available about the optimal interval for screening, making this a subject of debate. We associated the prevalence of advanced colorectal neoplasms with...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2009.10.054
更新日期：2010-03-01 00:00:00
abstract：:Endoscopic submucosal dissection was developed in Japan, early in this century, to provide a minimally invasive yet curative treatment for the large numbers of patients with early gastric cancer identified by the national screening program. Previously, the majority of these patients were treated surgically at substant...
journal_title：Gastroenterology
pub_type： 杂志文章,评审
doi：10.1053/j.gastro.2018.01.068
更新日期：2018-05-01 00:00:00
abstract：BACKGROUND & AIMS:Gastroesophageal reflux disease (GERD) interferes with the quality of life and carries an increased risk for esophageal adenocarcinoma. We investigated genetic influence in the development of reflux. METHODS:We compared concordance for reflux in monozygotic (MZ) and dizygotic (DZ) twins. All twins ag...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/gast.2002.30301
更新日期：2002-01-01 00:00:00
abstract：:The purpose of the study was to determine (a) the frequency and cause of mortality in patients with chronic pancreatitis; (b) the cumulative survival rates corrected by comparison of patients with a matched French population; and (c) the factors associated with mortality by a unidimensional and multidimensional analys...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1989-04-01 00:00:00
abstract：BACKGROUND & AIMS:We investigated the transcriptome of esophageal squamous cell carcinoma (ESCC) cells, activity of gene regulatory (enhancer and promoter regions), and the effects of blocking epigenetic regulatory proteins. METHODS:We performed chromatin immunoprecipitation sequencing with antibodies against H3K4me1,...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2020.06.050
更新日期：2020-10-01 00:00:00
abstract：BACKGROUND & AIMS:Helicobacter pylori attaches to mucin oligosaccharides that are expressed on host gastric epithelium. We used the rhesus macaque model to characterize the effect of H. pylori infection on gastric mucin oligosaccharides during acute and chronic infection. METHODS:Specific pathogen (H. pylori)-free rhe...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2009.04.014
更新日期：2009-09-01 00:00:00
abstract：BACKGROUND & AIMS:AJM300 is an orally active small-molecule antagonist of the α4 integrin subunit. We performed a randomized trial to investigate the efficacy and safety of AJM300 in patients with active ulcerative colitis (UC). METHODS:In a double-blind, placebo-controlled, phase 2a study, 102 patients with moderatel...
journal_title：Gastroenterology
pub_type： 评论,杂志文章
doi：10.1053/j.gastro.2015.08.044
更新日期：2015-12-01 00:00:00
abstract：:In vitro neurite outgrowth and neuronal survival are promoted by laminin, and neuronal migration is promoted by collagen type IV. This led to the hypothesis that Hirschsprung's disease (HD) could be the result of an abnormal extracellular matrix microenvironment in the affected bowel during embryogenesis. Using indire...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1992-04-01 00:00:00
abstract：:Receptor-dependent and -independent regulation of gastrin secretion from cultured human antral G cells was investigated. Human antral mucosal cell preparations that were enriched for G cells were obtained by sequential incubations with collagenase and ethylenediaminetetraacetic acid, centrifugal elutriation, and short...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(90)91226-v
更新日期：1990-07-01 00:00:00
abstract：:We have conducted a double-blind controlled trial of colchicine in patients with primary biliary cirrhosis. Fifty-seven patients with biopsy-proven primary biliary cirrhosis were randomized to receive either 0.6 mg of colchicine twice daily or an identically appearing placebo. Patients underwent clinical and laborator...
journal_title：Gastroenterology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/0016-5085(88)90300-9
更新日期：1988-07-01 00:00:00
abstract：:The diagnosis of inflammatory bowel disease (IBD) with its 2 main subforms, Crohn's disease and ulcerative colitis, is based on clinical, endoscopic, radiologic, and histologic criteria. This paradigm remains unchanged despite the advent of new molecular technologies for the examination of serum proteins and genetic s...
journal_title：Gastroenterology
pub_type： 杂志文章,评审
doi：10.1053/j.gastro.2007.09.001
更新日期：2007-11-01 00:00:00
abstract：:Twenty-seven carriers of the hepatitis B surface antigen who underwent liver transplantation in Italy and Belgium for terminal Hepatitis delta virus (HDV) cirrhosis were investigated. In 22 of the patients, HDV infection recurred. Two patients died of coexisting HDV and hepatitis B virus (HBV) reactivation. Four patie...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(91)90404-9
更新日期：1991-12-01 00:00:00
