Crystal structure of the motor domain of the kinesin-related motor ncd.


:Microtubule-based ATPases of the kinesin superfamily provide the motile force for many animated features of living cells. Kinesin motors differ in their direction of movement along microtubules. Kinesin and ncd, a kinesin-related motor involved in formation and maintenance of mitotic and meiotic spindles, move in opposite directions along microtubules, even though their motor domains are 40% identical in amino-acid sequence. Here we report the crystal structure of the MgADP complex of the Drosophila ncd motor domain determined to 2.5A by X-ray crystallography, and compare it to the kinesin structure. The ncd and kinesin motor domains are remarkably similar in structure, and the locations of conserved surface amino acids suggest these motors share a common microtubule-binding site. Moreover, structural and functional comparisons of ncd, kinesin, myosin and G proteins indicate that these NTPases may have a similar strategy of changing conformation between NTP and NDP states. We propose a general model for converting a common gamma-phosphate-sensing mechanism into opposite polarities of movement for kinesin and ncd.






Sablin EP,Kull FJ,Cooke R,Vale RD,Fletterick RJ




Has Abstract


1996-04-11 00:00:00














  • Bursts of retrotransposition reproduced in Arabidopsis.

    abstract::Retrotransposons, which proliferate by reverse transcription of RNA intermediates, comprise a major portion of plant genomes. Plants often change the genome size and organization during evolution by rapid proliferation and deletion of long terminal repeat (LTR) retrotransposons. Precise transposon sequences throughout...


    pub_type: 杂志文章


    authors: Tsukahara S,Kobayashi A,Kawabe A,Mathieu O,Miura A,Kakutani T

    更新日期:2009-09-17 00:00:00

  • A dispersive morph in the naked mole-rat.

    abstract::Close inbreeding is known for a variety of small mammal species for which a high probability of mortality during dispersal makes helping and delayed maturation a relatively secure fitness option. Prolonged inbreeding, however, is usually associated with lowered fitness, and it has been shown that most highly inbred sm...


    pub_type: 杂志文章


    authors: O'Riain MJ,Jarvis JU,Faulkes CG

    更新日期:1996-04-18 00:00:00

  • ATP-dependent inositide phosphorylation required for Ca(2+)-activated secretion.

    abstract::Regulated fusion of secretory granules with the plasma membrane in secretory cells requires ATP, Ca2+ and cytosolic as well as membrane proteins. ATP-dependent steps in Ca(2+)-activated secretion from PC12 cells require three cytosolic PEP proteins (priming in exocytosis proteins, PEP1-3), the identity of which will p...


    pub_type: 杂志文章


    authors: Hay JC,Fisette PL,Jenkins GH,Fukami K,Takenawa T,Anderson RA,Martin TF

    更新日期:1995-03-09 00:00:00

  • Selective development of CD4+ T cells in transgenic mice expressing a class II MHC-restricted antigen receptor.

    abstract::T lymphocytes are predisposed to recognition of foreign protein fragments bound to cell-surface molecules encoded by the major histocompatibility complex (MHC). There is now compelling evidence that this specificity is a consequence of a selection process operating on developing T lymphocytes in the thymus. As a resul...


    pub_type: 杂志文章


    authors: Kaye J,Hsu ML,Sauron ME,Jameson SC,Gascoigne NR,Hedrick SM

    更新日期:1989-10-26 00:00:00

  • Systematic variation of the stellar initial mass function in early-type galaxies.

    abstract::Much of our knowledge of galaxies comes from analysing the radiation emitted by their stars, which depends on the present number of each type of star in the galaxy. The present number depends on the stellar initial mass function (IMF), which describes the distribution of stellar masses when the population formed, and ...


    pub_type: 杂志文章


    authors: Cappellari M,McDermid RM,Alatalo K,Blitz L,Bois M,Bournaud F,Bureau M,Crocker AF,Davies RL,Davis TA,de Zeeuw PT,Duc PA,Emsellem E,Khochfar S,Krajnović D,Kuntschner H,Lablanche PY,Morganti R,Naab T,Oosterloo T,Sarz

    更新日期:2012-04-25 00:00:00

  • Electrogenic glutamate uptake in glial cells is activated by intracellular potassium.

    abstract::Uptake of glutamate into glial cells in the CNS maintains the extracellular glutamate concentration below neurotoxic levels and helps terminate its action as a neurotransmitter. The co-transport of two sodium ions on the glutamate carrier is thought to provide the energy needed to transport glutamate into cells. We ha...


    pub_type: 杂志文章


    authors: Barbour B,Brew H,Attwell D

    更新日期:1988-09-29 00:00:00

  • Host and viral traits predict zoonotic spillover from mammals.

    abstract::The majority of human emerging infectious diseases are zoonotic, with viruses that originate in wild mammals of particular concern (for example, HIV, Ebola and SARS). Understanding patterns of viral diversity in wildlife and determinants of successful cross-species transmission, or spillover, are therefore key goals f...


    pub_type: 杂志文章


    authors: Olival KJ,Hosseini PR,Zambrana-Torrelio C,Ross N,Bogich TL,Daszak P

    更新日期:2017-06-29 00:00:00

  • Microperiodic structures: direct writing of three-dimensional webs.

    abstract::Applications are emerging that require the creation of fine-scale structures in three dimensions--examples include scaffolds for tissue engineering, micro-fluidic devices and photonic materials that control light propagation over a range of frequencies. But writing methods such as dip-pen nanolithography and ink-jet p...


    pub_type: 杂志文章


    authors: Gratson GM,Xu M,Lewis JA

    更新日期:2004-03-25 00:00:00

  • How B and T cells talk to each other.

    abstract::The B cells of the immune system, which secrete antibodies against foreign antigens, are fully specific and effective only after maturation in lymph nodes and other lymphoid tissues. Immunocompetent T cells play a crucial part in this process, but the molecular details of the way in which the two cell types interact h...


    pub_type: 杂志文章,评审


    authors: Clark EA,Ledbetter JA

    更新日期:1994-02-03 00:00:00

  • Protein kinase B (c-Akt) in phosphatidylinositol-3-OH kinase signal transduction.

    abstract::A serine/threonine kinase, named protein kinase B (PKB) for its sequence homology to both protein kinase A and C, has previously been isolated. PKB, which is identical to the kinase Rac, was later found to be the cellular homologue of the transforming v-Akt. Here we show that PKB is activated by stimuli such as insuli...


    pub_type: 杂志文章


    authors: Burgering BM,Coffer PJ

    更新日期:1995-08-17 00:00:00

  • Extensive variation of human immunodeficiency virus type-1 in vivo.

    abstract::Genotypic variation among independent isolates of human immunodeficiency virus type-1 (HIV-1) is well known, but its molecular basis and biological consequences are poorly understood. We examined the genesis of molecular variation in HIV-1 by sequential virus isolations from two chronically infected individuals and an...


    pub_type: 杂志文章


    authors: Saag MS,Hahn BH,Gibbons J,Li Y,Parks ES,Parks WP,Shaw GM

    更新日期:1988-08-04 00:00:00

  • Telomerase reactivation reverses tissue degeneration in aged telomerase-deficient mice.

    abstract::An ageing world population has fuelled interest in regenerative remedies that may stem declining organ function and maintain fitness. Unanswered is whether elimination of intrinsic instigators driving age-associated degeneration can reverse, as opposed to simply arrest, various afflictions of the aged. Such instigator...


    pub_type: 杂志文章


    authors: Jaskelioff M,Muller FL,Paik JH,Thomas E,Jiang S,Adams AC,Sahin E,Kost-Alimova M,Protopopov A,Cadiñanos J,Horner JW,Maratos-Flier E,Depinho RA

    更新日期:2011-01-06 00:00:00

  • Structural and biochemical basis of apoptotic activation by Smac/DIABLO.

    abstract::Apoptosis (programmed cell death), an essential process in the development and homeostasis of metazoans, is carried out by caspases. The mitochondrial protein Smac/DIABLO performs a critical function in apoptosis by eliminating the inhibitory effect of IAPs (inhibitor of apoptosis proteins) on caspases. Here we show t...


    pub_type: 杂志文章


    authors: Chai J,Du C,Wu JW,Kyin S,Wang X,Shi Y

    更新日期:2000-08-24 00:00:00

  • Substituted ammonium ions as allergenic determinants in drug allergy.

    abstract::Serious, and occasionally fatal, anaphylactic-like (anaphylactoid) reactions may occur when a patient is exposed to a drug for the first time. Apart from the penicillins, nothing is known of the nature of antigenic or sensitizing drug determinants and, as yet, there is no evidence for the involvement of IgE antibodies...


    pub_type: 杂志文章


    authors: Baldo BA,Fisher MM

    更新日期:1983-11-17 00:00:00

  • The molecular structure of lecithin dihydrate.

    abstract::Lecithin is a major structural component of biological membranes. Because of their amphipathic nature, lecithin and related phospholipids tend to aggregate as bilayer structures in which the hydrophilic head groups are orientated towards the surface and the hydrophobic hydrocarbon chains towards the interior. A detail...


    pub_type: 杂志文章


    authors: Pearson RH,Pascher I

    更新日期:1979-10-11 00:00:00

  • Respiration as the main determinant of carbon balance in European forests.

    abstract::Carbon exchange between the terrestrial biosphere and the atmosphere is one of the key processes that need to be assessed in the context of the Kyoto Protocol. Several studies suggest that the terrestrial biosphere is gaining carbon, but these estimates are obtained primarily by indirect methods, and the factors that ...


    pub_type: 杂志文章


    authors: Valentini R,Matteucci G,Dolman AJ,Schulze ED,Rebmann C,Moors EJ,Granier A,Gross P,Jensen NO,Pilegaard K,Lindroth A,Grelle A,Bernhofer C,Grünwald T,Aubinet M,Ceulemans R,Kowalski AS,Vesala T,Rannik U,Berbigier P,Lo

    更新日期:2000-04-20 00:00:00

  • Sub-luminous type Ia supernovae from the mergers of equal-mass white dwarfs with mass approximately 0.9M[symbol: see text].

    abstract::Type Ia supernovae are thought to result from thermonuclear explosions of carbon-oxygen white dwarf stars. Existing models generally explain the observed properties, with the exception of the sub-luminous 1991bg-like supernovae. It has long been suspected that the merger of two white dwarfs could give rise to a type I...


    pub_type: 杂志文章


    authors: Pakmor R,Kromer M,Röpke FK,Sim SA,Ruiter AJ,Hillebrandt W

    更新日期:2010-01-07 00:00:00

  • Patterns and trends of Northern Hemisphere snow mass from 1980 to 2018.

    abstract::Warming surface temperatures have driven a substantial reduction in the extent and duration of Northern Hemisphere snow cover1-3. These changes in snow cover affect Earth's climate system via the surface energy budget, and influence freshwater resources across a large proportion of the Northern Hemisphere4-6. In contr...


    pub_type: 历史文章,杂志文章


    authors: Pulliainen J,Luojus K,Derksen C,Mudryk L,Lemmetyinen J,Salminen M,Ikonen J,Takala M,Cohen J,Smolander T,Norberg J

    更新日期:2020-05-01 00:00:00

  • Actin-based motility of vaccinia virus mimics receptor tyrosine kinase signalling.

    abstract::Studies of the actin-based motility of the intracellular pathogens Listeria monocytogenes and Shigella flexneri have provided important insight into the events occurring at the leading edges of motile cells. Like the bacteria Listeria and Shigella, vaccinia virus, a relative of the causative agent of smallpox, uses ac...


    pub_type: 杂志文章


    authors: Frischknecht F,Moreau V,Röttger S,Gonfloni S,Reckmann I,Superti-Furga G,Way M

    更新日期:1999-10-28 00:00:00

  • Piezoelectric and pyroelectric effects induced by interface polar symmetry.

    abstract::Interfaces in heterostructures have been a key point of interest in condensed-matter physics for decades owing to a plethora of distinctive phenomena-such as rectification1, the photovoltaic effect2, the quantum Hall effect3 and high-temperature superconductivity4-and their critical roles in present-day technical devi...


    pub_type: 杂志文章


    authors: Yang MM,Luo ZD,Mi Z,Zhao J,E SP,Alexe M

    更新日期:2020-08-01 00:00:00

  • Sterically controlled mechanochemistry under hydrostatic pressure.

    abstract::Mechanical stimuli can modify the energy landscape of chemical reactions and enable reaction pathways, offering a synthetic strategy that complements conventional chemistry. These mechanochemical mechanisms have been studied extensively in one-dimensional polymers under tensile stress using ring-opening and reorganiza...


    pub_type: 杂志文章


    authors: Yan H,Yang F,Pan D,Lin Y,Hohman JN,Solis-Ibarra D,Li FH,Dahl JEP,Carlson RMK,Tkachenko BA,Fokin AA,Schreiner PR,Galli G,Mao WL,Shen ZX,Melosh NA

    更新日期:2018-02-21 00:00:00

  • Arginine vasopressin enhances pHi regulation in the presence of HCO3- by stimulating three acid-base transport systems.

    abstract::Growth factors raise intracellular pH (pHi) by stimulating Na+/H+ exchange in the absence of HCO3-. In mutant cells that lack the Na+/H+ exchange activity, this alkalinization does not occur, and the cells do not proliferate without artificial elevation of pHi. It has therefore been widely suggested that an early pHi ...


    pub_type: 杂志文章


    authors: Ganz MB,Boyarsky G,Sterzel RB,Boron WF

    更新日期:1989-02-16 00:00:00

  • Structured spheres generated by an in-fibre fluid instability.

    abstract::From drug delivery to chemical and biological catalysis and cosmetics, the need for efficient fabrication pathways for particles over a wide range of sizes, from a variety of materials, and in many different structures has been well established. Here we harness the inherent scalability of fibre production and an in-fi...


    pub_type: 杂志文章


    authors: Kaufman JJ,Tao G,Shabahang S,Banaei EH,Deng DS,Liang X,Johnson SG,Fink Y,Abouraddy AF

    更新日期:2012-07-26 00:00:00

  • Mapping of the class II region of the human major histocompatibility complex by pulsed-field gel electrophoresis.

    abstract::The class II region of the human major histocompatibility complex (MHC) encodes a polymorphic set of cell surface glycoproteins involved in the regulation of the immune response. Each glycoprotein is a heterodimer composed of a alpha-chain of relative molecular mass (Mr) 34,000 (34 K) and a beta-chain of Mr = 28K. The...


    pub_type: 杂志文章


    authors: Hardy DA,Bell JI,Long EO,Lindsten T,McDevitt HO

    更新日期:1986-10-02 00:00:00

  • Metastasis suppressor gene KiSS-1 encodes peptide ligand of a G-protein-coupled receptor.

    abstract::Metastasis is a major cause of death in cancer patients and involves a multistep process including detachment of cancer cells from a primary cancer, invasion of surrounding tissue, spread through circulation, re-invasion and proliferation in distant organs. KiSS-1 is a human metastasis suppressor gene, that suppresses...


    pub_type: 杂志文章


    authors: Ohtaki T,Shintani Y,Honda S,Matsumoto H,Hori A,Kanehashi K,Terao Y,Kumano S,Takatsu Y,Masuda Y,Ishibashi Y,Watanabe T,Asada M,Yamada T,Suenaga M,Kitada C,Usuki S,Kurokawa T,Onda H,Nishimura O,Fujino M

    更新日期:2001-05-31 00:00:00

  • Mars rover, French research integrity and NIPS renamed.

    abstract:: ...


    pub_type: 新闻



    更新日期:2018-11-01 00:00:00

  • A 'don't eat me' immune signal protects neuronal connections.

    abstract:: ...


    pub_type: 评论,新闻


    authors: Rivest S

    更新日期:2018-11-01 00:00:00

  • The genetics of monarch butterfly migration and warning colouration.

    abstract::The monarch butterfly, Danaus plexippus, is famous for its spectacular annual migration across North America, recent worldwide dispersal, and orange warning colouration. Despite decades of study and broad public interest, we know little about the genetic basis of these hallmark traits. Here we uncover the history of t...


    pub_type: 杂志文章


    authors: Zhan S,Zhang W,Niitepõld K,Hsu J,Haeger JF,Zalucki MP,Altizer S,de Roode JC,Reppert SM,Kronforst MR

    更新日期:2014-10-16 00:00:00

  • Messenger RNA for the coat protein of tobacco mosaic virus.

    abstract::TMV RNA is not an efficient template for translation of the viral coat protein, in spite of containing nucleotide sequences coding for the protein. Efficient translation requires the prior synthesis within infected cells of a smaller RNA carrying only a portion of the information encoded in the whole genome. ...


    pub_type: 杂志文章


    authors: Hunter TR,Hunt T,Knowland J,Zimmern D

    更新日期:1976-04-29 00:00:00

  • Transformation and diversification in early mammal evolution.

    abstract::Evolution of the earliest mammals shows successive episodes of diversification. Lineage-splitting in Mesozoic mammals is coupled with many independent evolutionary experiments and ecological specializations. Classic scenarios of mammalian morphological evolution tend to posit an orderly acquisition of key evolutionary...


    pub_type: 历史文章,杂志文章,评审


    authors: Luo ZX

    更新日期:2007-12-13 00:00:00