Abstract：
:Microtubule-based ATPases of the kinesin superfamily provide the motile force for many animated features of living cells. Kinesin motors differ in their direction of movement along microtubules. Kinesin and ncd, a kinesin-related motor involved in formation and maintenance of mitotic and meiotic spindles, move in opposite directions along microtubules, even though their motor domains are 40% identical in amino-acid sequence. Here we report the crystal structure of the MgADP complex of the Drosophila ncd motor domain determined to 2.5A by X-ray crystallography, and compare it to the kinesin structure. The ncd and kinesin motor domains are remarkably similar in structure, and the locations of conserved surface amino acids suggest these motors share a common microtubule-binding site. Moreover, structural and functional comparisons of ncd, kinesin, myosin and G proteins indicate that these NTPases may have a similar strategy of changing conformation between NTP and NDP states. We propose a general model for converting a common gamma-phosphate-sensing mechanism into opposite polarities of movement for kinesin and ncd.
journal_name
Naturejournal_title
Natureauthors
Sablin EP,Kull FJ,Cooke R,Vale RD,Fletterick RJdoi
10.1038/380555a0subject
Has Abstractpub_date
1996-04-11 00:00:00pages
555-9issue
6574eissn
0028-0836issn
1476-4687journal_volume
380pub_type
杂志文章相关文献
NATURE文献大全abstract：:The three-dimensional structure of the 66-amino acid cro repressor protein of bacteriophage lambda suggests how it binds to its operator DNA. We propose that a dimer of cro protein is bound to the B-form of DNA with the 2-fold axis of the dimer coincident with the 2-fold axis of DNA. A pair of 2-fold-related alpha-hel...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/290754a0
更新日期：1981-04-30 00:00:00
abstract：:Nitrogen (N) limits the productivity of many ecosystems worldwide, thereby restricting the ability of terrestrial ecosystems to offset the effects of rising atmospheric CO(2) emissions naturally. Understanding input pathways of bioavailable N is therefore paramount for predicting carbon (C) storage on land, particular...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature10415
更新日期：2011-08-31 00:00:00
abstract：:Major histocompatibility complex class II molecules and their peptide ligands show unusual interaction kinetics, with slow association and dissociation rates that yield an apparent equilibrium constant of approximately 10(-6)-10(-8) M (refs 1-5). However, there is evidence for a specific, rapidly formed, short-lived c...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/370647a0
更新日期：1994-08-25 00:00:00
abstract：:The search for the earliest fossil evidence of the human lineage has been concentrated in East Africa. Here we report the discovery of six hominid specimens from Chad, central Africa, 2,500 km from the East African Rift Valley. The fossils include a nearly complete cranium and fragmentary lower jaws. The associated fa...
journal_title：Nature
pub_type： 历史文章,杂志文章
doi：10.1038/nature00879
更新日期：2002-07-11 00:00:00
abstract：:Ecological and societal disruptions by modern climate change are critically determined by the time frame over which climates shift beyond historical analogues. Here we present a new index of the year when the projected mean climate of a given location moves to a state continuously outside the bounds of historical vari...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature12540
更新日期：2013-10-10 00:00:00
abstract：:Abyssal-hill-bounding faults that pervade the oceanic crust are the most common tectonic feature on the surface of the Earth. The recognition that these faults form at plate spreading centres came with the plate tectonic revolution. Recent observations reveal a large range of fault sizes and orientations; numerical mo...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature03358
更新日期：2005-04-07 00:00:00
abstract：:Mice express a collection of superantigens, which bind to class II major histocompatibility proteins and interact with T cells bearing particular V beta chains as part of their alpha beta receptors. These superantigens have been suggested to be encoded by exogenous or endogenous mouse mammary tumour viruses. One such ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/350203a0
更新日期：1991-03-21 00:00:00
abstract：:We have attempted to reproduce the findings of Benveniste and co-workers, who reported in 1988 that degranulation of human basophil leukocytes is triggered by very dilute (10(2)-10(120)) antiserum against IgE. The results were contrary to conventional scientific theory and were not satisfactorily explained. Following ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/366525a0
更新日期：1993-12-09 00:00:00
abstract：:Humans and other animals must often make decisions on the basis of imperfect evidence. Statisticians use measures such as P values to assign degrees of confidence to propositions, but little is known about how the brain computes confidence estimates about decisions. We explored this issue using behavioural analysis an...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature07200
更新日期：2008-09-11 00:00:00
abstract：:The rare Egyptian vulture (Neophron percnopterus) stands out among the Old World vultures (Family Accipitridae) because of its brightly ornamented head, which is coloured yellow by carotenoid pigments, and its practice of feeding on faeces. Here we show that Egyptian vultures obtain these pigments from the excrement o...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/416807a
更新日期：2002-04-25 00:00:00
abstract：:Rac signalling to actin -- a pathway that is thought to be mediated by the protein Scar/WAVE (WASP (Wiskott-Aldrich syndrome protein)-family verprolin homologous protein -- has a principal role in cell motility. In an analogous pathway, direct interaction of Cdc42 with the related protein N-WASP stimulates actin polym...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature00859
更新日期：2002-08-15 00:00:00
abstract：:Werner's syndrome (WS) is a rare autosomal recessive disease in which the affected individuals display symptoms of premature ageing. The substantial phenotypic overlap between WS and normal ageing indicates that these two conditions may have pathogenetic mechanisms in common. The WS mutation has pleiotropic effects, a...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/355735a0
更新日期：1992-02-20 00:00:00
abstract：:Quantum light is characterized by distinctive statistical distributions that are possible only because of quantum mechanical effects. For example, single photons and correlated photon pairs exhibit photon number distributions with variance lower than classically allowed limits. This enables high-fidelity transmission ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-018-0478-3
更新日期：2018-09-01 00:00:00
abstract：:The 18-base-pair sequence element AGGTCGACCAGTACTCCG (the Sal box) signals termination of mouse ribosomal gene transcription. This sequence is recognized by a sequence-specific DNA-binding protein, TTF I, which mediates the termination of transcription by RNA polymerase I (pol I). Subsequently, the ends of the primary...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/344559a0
更新日期：1990-04-05 00:00:00
abstract：:L-glutamate, the neurotransmitter of the majority of excitatory synapses in the brain, acts on three classes of ionotropic receptors: NMDA (N-methyl-D-aspartate), AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid) and kainate receptors. Little is known about the physiological role of kainate receptors be...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/33408
更新日期：1998-04-09 00:00:00
abstract：:In the few species of birds in which males form display partnerships to attract females, one male secures most or all of the copulations. This leads to the question of why subordinate males help in the absence of observable reproductive benefits. Hamilton's concept of kin selection, whereby individuals can benefit ind...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature03325
更新日期：2005-03-03 00:00:00
abstract：:Gram-negative bacteria, such as Escherichia coli, expel toxic chemicals through tripartite efflux pumps that span both the inner and outer membrane. The three parts are an inner membrane, substrate-binding transporter; a membrane fusion protein; and an outer-membrane-anchored channel. The fusion protein connects the t...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature09743
更新日期：2011-02-24 00:00:00
abstract：:L-GLUTAMATE and L-aspartate are thought to have a widespread function as synaptic transmitters in the mammalian central nervous system and there are at least three types of neuronal glutamate receptors, which can be activated by the selective agonists N-methyl-D-aspartate (NMDA), quisqualate and kainate. Recent experi...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/339380a0
更新日期：1989-06-01 00:00:00
abstract：:In the absence of extraneously added template, standard preparations of Q beta replicase spontaneously synthesize RNA in vitro, possibly as a result of RNA contamination. Using special enzyme purifications, Sumper and Luce presented evidence that self-replicating RNA not present ab initio can grow out of 'template-fre...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/321089a0
更新日期：1986-05-01 00:00:00
abstract：:Peroxisomes are among the intracellular organelles of eukaryotic cells that contain specialized sets of enzymes with specific functions. Little is known of membranous components involved in assembly of the intracellular compartments. We isolated two peroxisome-deficient and mutually complementary, Chinese hamster ovar...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/350077a0
更新日期：1991-03-07 00:00:00
abstract：:Quantum computers are expected to outperform conventional computers in several important applications, from molecular simulation to search algorithms, once they can be scaled up to large numbers-typically millions-of quantum bits (qubits)1-3. For most solid-state qubit technologies-for example, those using superconduc...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-020-2171-6
更新日期：2020-04-01 00:00:00
abstract：:The molecular mechanisms of exon definition and back-splicing are fundamental unanswered questions in pre-mRNA splicing. Here we report cryo-electron microscopy structures of the yeast spliceosomal E complex assembled on introns, providing a view of the earliest event in the splicing cycle that commits pre-mRNAs to sp...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-019-1523-6
更新日期：2019-09-01 00:00:00
abstract：: ...
journal_title：Nature
pub_type： 评论,新闻
doi：10.1038/d41586-018-07008-6
更新日期：2018-11-01 00:00:00
abstract：:The kinesin superfamily is a class of microtubule-based mechano-enzymes involved in intracellular transport and chromosome movements. Molecules that move towards either the plus end or the minus end of microtubules are represented within the family. The motor domains of these molecules exhibit considerable sequence ho...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/376271a0
更新日期：1995-07-20 00:00:00
abstract：:An important step towards understanding the molecular basis of the functional diversity of voltage-gated K+ channels in the mammalian brain has been the discovery of a family of genes encoding rat brain K+ channel-forming (RCK) proteins. All species of these RCK proteins form homomultimeric voltage-gated K+ channels w...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/345535a0
更新日期：1990-06-07 00:00:00
abstract：:X chromosomes evolve differently from autosomes, but general governing principles have not emerged. For example, genes with male-biased expression are under-represented on the X chromosome of D. melanogaster, but are randomly distributed in the genome of Anopheles gambiae. In direct global profiling experiments using ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature06330
更新日期：2007-11-08 00:00:00
abstract：:Mature cortical pyramidal neurons receive excitatory inputs onto small protrusions emanating from their dendrites called spines. Spines undergo activity-dependent remodelling, stabilization and pruning during development, and similar structural changes can be triggered by learning and changes in sensory experiences. H...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature09986
更新日期：2011-06-02 00:00:00
abstract：:Surface melt on the Greenland ice sheet has shown increasing trends in areal extent and duration since the beginning of the satellite era. Records for melt were broken in 2005, 2007, 2010 and 2012. Much of the increased surface melt is occurring in the percolation zone, a region of the accumulation area that is perenn...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature11566
更新日期：2012-11-08 00:00:00
abstract：:Polymeric matrices can be used to grow new tissues and organs, and the delivery of growth factors from these matrices is one method to regenerate tissues. A problem with engineering tissues that exist in a mechanically dynamic environment, such as bone, muscle and blood vessels, is that most drug delivery systems have...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/35050141
更新日期：2000-12-21 00:00:00
abstract：:Novel capsomeric complexes, pentamers and hexamers were detected as chemical entities in phage Q beta. Both were composed of identical protein subunits and stabilized by intermolecular disulphide bonds. Their numbers per particle were about 12 for pentamers and about 20 for hexamers--consistent with theoretical expect...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/298819a0
更新日期：1982-08-26 00:00:00
