Crystal structure of the motor domain of the kinesin-related motor ncd.


:Microtubule-based ATPases of the kinesin superfamily provide the motile force for many animated features of living cells. Kinesin motors differ in their direction of movement along microtubules. Kinesin and ncd, a kinesin-related motor involved in formation and maintenance of mitotic and meiotic spindles, move in opposite directions along microtubules, even though their motor domains are 40% identical in amino-acid sequence. Here we report the crystal structure of the MgADP complex of the Drosophila ncd motor domain determined to 2.5A by X-ray crystallography, and compare it to the kinesin structure. The ncd and kinesin motor domains are remarkably similar in structure, and the locations of conserved surface amino acids suggest these motors share a common microtubule-binding site. Moreover, structural and functional comparisons of ncd, kinesin, myosin and G proteins indicate that these NTPases may have a similar strategy of changing conformation between NTP and NDP states. We propose a general model for converting a common gamma-phosphate-sensing mechanism into opposite polarities of movement for kinesin and ncd.






Sablin EP,Kull FJ,Cooke R,Vale RD,Fletterick RJ




Has Abstract


1996-04-11 00:00:00














  • Sequence of protein disulphide isomerase and implications of its relationship to thioredoxin.

    abstract::The formation of disulphide bonds is essential to the structure and function of proteins. These bonds rapidly form either cotranslationally or immediately post-translationally in the lumen of the endoplasmic reticulum. Native disulphide pairing for such proteins has been achieved in vitro; however, the rates of reasse...


    pub_type: 杂志文章


    authors: Edman JC,Ellis L,Blacher RW,Roth RA,Rutter WJ

    更新日期:1985-09-19 00:00:00

  • A protein from human placental nuclei binds preferentially to 5-methylcytosine-rich DNA.

    abstract::The methylation of vertebrate DNA at the 5-position of approximately 3-10% of its cytosine residues occurs in a sequence-specific and tissue-specific manner and has been implicated in the control of transcription. How these differences are established and how they mediate the initiation or maintenance of transcription...


    pub_type: 杂志文章


    authors: Huang LH,Wang R,Gama-Sosa MA,Shenoy S,Ehrlich M

    更新日期:1984-03-15 00:00:00

  • A genetically augmented future.

    abstract:: ...


    pub_type: 杂志文章


    authors: Clayton EW

    更新日期:2018-12-01 00:00:00

  • Functional innervation of cultured hippocampal neurones by cholinergic afferents from co-cultured septal explants.

    abstract::The rat hippocampus receives a strong cholinergic innervation from the medial septum; information about the development and function of this pathway could help to elucidate the mechanisms of memory functions. Previous electrophysiological studies have shown that septal stimulation in vivo facilitates commissural and p...


    pub_type: 杂志文章


    authors: Gahwiler BH,Brown DA

    更新日期:1985-02-14 00:00:00

  • Environment and host as large-scale controls of ectomycorrhizal fungi.

    abstract::Explaining the large-scale diversity of soil organisms that drive biogeochemical processes-and their responses to environmental change-is critical. However, identifying consistent drivers of belowground diversity and abundance for some soil organisms at large spatial scales remains problematic. Here we investigate a m...


    pub_type: 杂志文章


    authors: van der Linde S,Suz LM,Orme CDL,Cox F,Andreae H,Asi E,Atkinson B,Benham S,Carroll C,Cools N,De Vos B,Dietrich HP,Eichhorn J,Gehrmann J,Grebenc T,Gweon HS,Hansen K,Jacob F,Kristöfel F,Lech P,Manninger M,Martin J

    更新日期:2018-06-01 00:00:00

  • Regulated progression of a cultured pre-B-cell line to the B-cell stage.

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    pub_type: 杂志文章


    authors: Reth MG,Ammirati P,Jackson S,Alt FW

    更新日期:1985-09-26 00:00:00

  • Low-temperature hydrogen production from water and methanol using Pt/α-MoC catalysts.

    abstract::Polymer electrolyte membrane fuel cells (PEMFCs) running on hydrogen are attractive alternative power supplies for a range of applications, with in situ release of the required hydrogen from a stable liquid offering one way of ensuring its safe storage and transportation before use. The use of methanol is particularly...


    pub_type: 杂志文章


    authors: Lin L,Zhou W,Gao R,Yao S,Zhang X,Xu W,Zheng S,Jiang Z,Yu Q,Li YW,Shi C,Wen XD,Ma D

    更新日期:2017-04-06 00:00:00

  • Solvent-induced distortions and the curvature of alpha-helices.

    abstract::The alpha-helix defined in 1951 by Pauling et al. on the basis of model building and X-ray fibre diffraction data has 3.65 residues per turn (n) achieved with planar peptides, torsion angles of phi = -48 degrees and psi = -57 degrees and hydrogen bonds which are close to linear. Although X-ray analyses of proteins hav...


    pub_type: 杂志文章


    authors: Blundell T,Barlow D,Borkakoti N,Thornton J

    更新日期:1983-11-17 00:00:00

  • 1970s and 'Patient 0' HIV-1 genomes illuminate early HIV/AIDS history in North America.

    abstract::The emergence of HIV-1 group M subtype B in North American men who have sex with men was a key turning point in the HIV/AIDS pandemic. Phylogenetic studies have suggested cryptic subtype B circulation in the United States (US) throughout the 1970s and an even older presence in the Caribbean. However, these temporal an...


    pub_type: 历史文章,杂志文章


    authors: Worobey M,Watts TD,McKay RA,Suchard MA,Granade T,Teuwen DE,Koblin BA,Heneine W,Lemey P,Jaffe HW

    更新日期:2016-11-03 00:00:00

  • Genetic contributions to stability and change in intelligence from childhood to old age.

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    pub_type: 杂志文章


    authors: Deary IJ,Yang J,Davies G,Harris SE,Tenesa A,Liewald D,Luciano M,Lopez LM,Gow AJ,Corley J,Redmond P,Fox HC,Rowe SJ,Haggarty P,McNeill G,Goddard ME,Porteous DJ,Whalley LJ,Starr JM,Visscher PM

    更新日期:2012-01-18 00:00:00

  • Endothelins are vascular-derived axonal guidance cues for developing sympathetic neurons.

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    pub_type: 杂志文章


    authors: Makita T,Sucov HM,Gariepy CE,Yanagisawa M,Ginty DD

    更新日期:2008-04-10 00:00:00

  • Developmental basis of limblessness and axial patterning in snakes.

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    pub_type: 杂志文章


    authors: Cohn MJ,Tickle C

    更新日期:1999-06-03 00:00:00

  • Isolation of a spermatozoid-releasing and -attracting substance from female gametophytes of Laminaria digitata.

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    pub_type: 杂志文章


    authors: Müller DG,Gassmann G,Lüning K

    更新日期:1979-05-31 00:00:00

  • A membrane form of guanylate cyclase is an atrial natriuretic peptide receptor.

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    pub_type: 杂志文章


    authors: Chinkers M,Garbers DL,Chang MS,Lowe DG,Chin HM,Goeddel DV,Schulz S

    更新日期:1989-03-02 00:00:00

  • Suppressor effects and cyclic AMP accumulation by the CD29 molecule of CD4+ lymphocytes.

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    pub_type: 杂志文章


    authors: Groux H,Huet S,Valentin H,Pham D,Bernard A

    更新日期:1989-05-11 00:00:00

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    pub_type: 杂志文章


    authors: Lottermoser T,Lonkai T,Amann U,Hohlwein D,Ihringer J,Fiebig M

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    pub_type: 信件


    authors: Gack MU,Shin YC,Joo CH,Urano T,Liang C,Sun L,Takeuchi O,Akira S,Chen Z,Inoue S,Jung JU

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    pub_type: 杂志文章


    authors: Feng J,Hennig RG,Ashcroft NW,Hoffmann R

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    pub_type: 评论,杂志文章


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    pub_type: 杂志文章


    authors: Liang J,Balachandra S,Ngo S,O'Brien LE

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  • A unique regulatory phase of DNA methylation in the early mammalian embryo.

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    pub_type: 杂志文章


    authors: Archibald RF,Kaspi VM,Ng CY,Gourgouliatos KN,Tsang D,Scholz P,Beardmore AP,Gehrels N,Kennea JA

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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Resulaj A,Kiani R,Wolpert DM,Shadlen MN

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  • Evolution of the MHC class I genes of a New World primate from ancestral homologues of human non-classical genes.

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    pub_type: 杂志文章


    authors: Watkins DI,Chen ZW,Hughes AL,Evans MG,Tedder TF,Letvin NL

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    pub_type: 杂志文章


    authors: Liu S,Kandoth PK,Warren SD,Yeckel G,Heinz R,Alden J,Yang C,Jamai A,El-Mellouki T,Juvale PS,Hill J,Baum TJ,Cianzio S,Whitham SA,Korkin D,Mitchum MG,Meksem K

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  • Mechanism of transfer RNA maturation by CCA-adding enzyme without using an oligonucleotide template.

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    pub_type: 杂志文章


    authors: Xiong Y,Steitz TA

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    pub_type: 杂志文章


    authors: Comas D,Petit F,Preat T

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  • The hydrodynamics of water strider locomotion.

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    pub_type: 杂志文章


    authors: Hu DL,Chan B,Bush JW

    更新日期:2003-08-07 00:00:00

  • Activation of transcription by two factors that bind promoter and enhancer sequences of the human metallothionein gene and SV40.

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    pub_type: 杂志文章


    authors: Lee W,Haslinger A,Karin M,Tjian R

    更新日期:1987-01-22 00:00:00