Effects of interferon-gamma on cell differentiation and cytokine production of a human monoblast cell line, U937.


:The U937 cell, a human monoblast cell line, has been used as a model to study the function of human monocytes. We investigated the effects of interferon-gamma (IFN-gamma) on superoxide anion (O2-) production, cell surface antigens, and cytokine production of U937 cells. IFN-gamma treatment enhanced O2- production of fMLP or PMA-stimulated U937 cells. IFN-gamma increased the ratio of CD23 and CD11b positive cells. The fluorescence intensity of CD14 and CD25 was enhanced by IFN-gamma treatment. U937 cells produced IL-1 alpha, IL-1 beta, IL-6, and TNF-alpha by lipopolysaccharide (LPS) stimulation. IFN-gamma treatment enhanced TNF-alpha production, but decreased IL-6 production. These results suggest that IFN-gamma differentiates U937 cells to monocyte-like cells and it regulates the production systems of IL-6 and TNF-alpha separately in U937 cells.






Ishizuka T,Hirata I,Adachi M,Kurimoto F,Hisada T,Dobashi K,Mori M




Has Abstract


1995-12-01 00:00:00












  • Leukotriene production associated with leukocyte membrane destruction: evidence of a terminal signal.

    abstract::Four methods of severe disruption of human neutrophil membrane integrity resulted in the formation of substantial amounts of leukotriene B4 and its isomers. This synthesis took place from endogenous substrate in the absence of any cell activators. It is postulated that leukotriene formation could be a preserved cell f...


    pub_type: 杂志文章


    authors: Green FA

    更新日期:1988-04-01 00:00:00

  • Polynitroxylated starch/TPL attenuates cachexia and increased epithelial permeability associated with TNBS colitis.

    abstract::Free radicals play an important role in the initiation and progression of inflammatory bowel disease (IBD). Therefore, the reduction or elimination of adverse oxidant effects can provide novel therapy for IBD. Here, the antioxidant capacity and protective effects of a new class of chemically modified hetastarch (polyn...


    pub_type: 杂志文章


    authors: Park JH,Ma L,Oshima T,Carter P,Coe L,Ma JW,Specian R,Grisham MB,Trimble CE,Hsia CJ,Liu JE,Alexander JS

    更新日期:2002-02-01 00:00:00

  • Electro-acupuncture Pretreatment at Zusanli (ST36) Acupoint Attenuates Lipopolysaccharide-Induced Inflammation in Rats by Inhibiting Ca2+ Influx Associated with Cannabinoid CB2 Receptors.

    abstract::In this study, we aimed to investigate the effect of electro-acupuncture (EA) pretreatment at zusanli (ST36) acupoint on lipopolysaccharide (LPS)-induced endotoxemic rat model and explore the underlying molecular mechanisms. Rats were treated with EA at ST36 for 7 days before being subjected to LPS. Two hours post-LPS...


    pub_type: 杂志文章


    authors: Chen T,Xiong Y,Long M,Zheng D,Ke H,Xie J,Yin N,Chen Z

    更新日期:2019-02-01 00:00:00

  • Thromboxane-induced neutrophil adhesion to pulmonary microvascular and aortic endothelium is regulated by CD18.

    abstract::Thromboxane (Tx) A2 generation and subsequent selective pulmonary sequestration of neutrophils (PMNs) is characteristic of several forms of the adult respiratory distress syndrome (ARDS). Therefore, we examined PMN-dependent adhesion to cultured pulmonary microvessel and aortic endothelium (EC) in response to U46,619 ...


    pub_type: 杂志文章


    authors: Wiles ME,Welbourn R,Goldman G,Hechtman HB,Shepro D

    更新日期:1991-06-01 00:00:00

  • Heat shock and proinflammatory stressors induce differential localization of heat shock proteins in human monocytes.

    abstract::Heat shock (HS) proteins (HSP) are a family of molecular chaperones induced by environmental stresses such as oxidative injury, and contribute to protection from and adaptation to cellular stress. We investigated in human monocytes the expression and subcellular distribution of hsp70 and hsc70 after HS and inflammatio...


    pub_type: 杂志文章


    authors: Mariéthoz E,Jacquier-Sarlin MR,Multhoff G,Healy AM,Tacchini-Cottier F,Polla BS

    更新日期:1997-12-01 00:00:00

  • Nicotine induces pro-inflammatory response in aortic vascular smooth muscle cells through a NFκB/osteopontin amplification loop-dependent pathway.

    abstract::Nicotine has anti- and pro-inflammatory properties in various cells. Its role in aortic vascular smooth muscle cells (VSMC) was explored. Human aortic VSMC were cultured. After nicotine (1.0 μM) and/or pyrrolidinedithiocarbamic acid (PDTC, 50 μM) treatment, the activation of nuclear factor κB (NFκB) was investigated. ...


    pub_type: 杂志文章


    authors: Wang Y,Zhang F,Yang W,Xue S

    更新日期:2012-02-01 00:00:00

  • Antiinflammation and Antioxidant Effects of Thalidomide on Pulmonary Fibrosis in Mice and Human Lung Fibroblasts.

    abstract::In this study, the potential effects of thalidomide (Thal) on bleomycin (BLM)-induced pulmonary fibrosis were investigated. BALB/C mice model of pulmonary fibrosis induced by an intratracheal instillation of BLM was adopted, and then was intraperitoneally injected with Thal (10, 20, 50 mg/kg) daily for 8 days, while t...


    pub_type: 杂志文章


    authors: Dong X,Li X,Li M,Chen M,Fan Q,Wei W

    更新日期:2017-12-01 00:00:00

  • Transient endotoxemia during burn wound revision causes leukocyte beta 2 integrin up-regulation and cytokine release.

    abstract::After severe burns, a wound revision is often done to remove devitalized tissue and minimize bacterial growth. After such revision, the patient may show signs of sepsis. In a group of burned patients we found a transient endotoxemia, and a subsequent leukocyte activation, monitored as increased expression of the beta ...


    pub_type: 杂志文章


    authors: Ljunghusen O,Berg S,Hed J,Lundahl J,Nettelblad H,Sjögren F,Stendahl O

    更新日期:1995-08-01 00:00:00

  • Steroids decrease granulocyte membrane fluidity, while phorbol ester increases membrane fluidity. Studies using electron paramagnetic resonance.

    abstract::High concentrations of corticosteroids inhibit granulocyte responses and disrupt agonist receptor function. Dose-response and time-course considerations make it unlikely that these effects are mediated via the glucocorticoid receptor, a concept further supported by the ability of sex steroids to work similar effects. ...


    pub_type: 杂志文章


    authors: Lamche HR,Silberstein PT,Knabe AC,Thomas DD,Jacob HS,Hammerschmidt DE

    更新日期:1990-02-01 00:00:00

  • Attenuation of acute inflammatory effects of silica in rat lung by 21-aminosteroid, U74389G.

    abstract::Chemical alteration of the glucocorticoid, methylprednisolone, has led to the introduction of a new class of compounds called the 21-aminosteroids (21-ASs). The purpose of this study was to investigate the effect of the 21-AS, U74389G, on silica-induced acute lung injury. Male Fischer 344 rats were treated intraperito...


    pub_type: 杂志文章


    authors: Antonini JM,van Dyke K,DiMatteo M,Reasor MJ

    更新日期:1995-02-01 00:00:00

  • Combination of Enteral Nutrition and Probiotics Promote Recovery Following Ileal Pouch-Anal Anastomosis in Rats.

    abstract::Many patients with ulcerative colitis suffer from malnutrition and intestinal flora disorders, which affect the postoperative intestinal barrier function of the ileal pouch. This study aimed to investigate the effects of enteral nutrition combined with probiotics after ileal pouch-anal anastomosis in rats. Male Spragu...


    pub_type: 杂志文章


    authors: Zheng T,Gao Y,Xu Y,Chen Z,Wang X,Liu J,Liu G

    更新日期:2020-11-05 00:00:00

  • Postnatal development of hepatic innate immune response.

    abstract::The liver is an immunocompetent organ that plays a key role in the immune response to infections, and the development of hepatic immune function during early postnatal stages has not been thoroughly characterized. This study analyzed the constitutive expression of complement factors, namely C3 and C9, and pattern reco...


    pub_type: 杂志文章


    authors: Le Rouzic V,Corona J,Zhou H

    更新日期:2011-12-01 00:00:00

  • Role of Leptin and SOCS3 in Inhibiting the Type I Interferon Response During Obesity.

    abstract::Obesity provokes an imbalance in the immune system, including an aberrant type I interferon response during some viral infections and after TLR stimulation. SOCS3 overexpression and altered systemic leptin levels could be responsible for the reduced type I interferon production in people with obesity and, eventually, ...


    pub_type: 杂志文章


    authors: Terán-Cabanillas E,Hernández J

    更新日期:2017-02-01 00:00:00

  • Alleviation of Atopic Dermatitis Lesions by a Benzylideneacetophenone Derivative via the MAPK Signaling Pathway.

    abstract::This experiment was conducted to investigate the effects of a benzylideneacetophenone derivative ((2E)-3-(4-hydroxy-3-methoxyphenyl)phenylpro-2-en-l-one (JC3)) on trimellitic anhydride (TMA)-induced atopic dermatitis (AD)-like symptoms in mice. To induce AD, the dorsal skins of mice were treated with 5% TMA on day 0 a...


    pub_type: 杂志文章


    authors: Sur B,Kang S,Kim M,Oh S

    更新日期:2019-06-01 00:00:00

  • Hydrogen peroxide induces DNA single strand breaks in respiratory epithelial cells.

    abstract::The respiratory epithelium is often exposed to oxidant gases, including ozone from photochemical smog and toxic oxygen metabolites released from neutrophils recruited in conditions of airway inflammation. We evaluated DNA single strand break formation by alkaline elution as a measure of oxidant-induced DNA damage to b...


    pub_type: 杂志文章


    authors: McDonald RJ,Pan LC,St George JA,Hyde DM,Ducore JM

    更新日期:1993-12-01 00:00:00

  • Effect of recombinant human interleukin 2 on neutrophil adherence to endothelial cells in vitro.

    abstract::Human neutrophils were demonstrated to possess interleukin-2 receptor (IL-2R) beta and gamma chains, not alpha chain and the binding of IL-2 to the IL-2R beta chain on neutrophils plays an important regulatory role in neutrophil functions. We have investigated in this study the hypothesis that recombinant human IL-2 (...


    pub_type: 杂志文章


    authors: Li J,Gyorffy S,Lee S,Kwok CS

    更新日期:1996-08-01 00:00:00

  • The Effects of Molecular Hydrogen and Suberoylanilide Hydroxamic Acid on Paraquat-Induced Production of Reactive Oxygen Species and TNF-α in Macrophages.

    abstract::The aim of this study is to investigate the effects of molecular hydrogen (H2) and suberoylanilide hydroxamic acid (SAHA), a histone deacetylase inhibitor, on paraquat (PQ)-stimulated production of reactive oxygen species (ROS) and tumor necrosis factor alpha (TNF-α) in macrophages. First, the PQ optimal concentration...


    pub_type: 杂志文章


    authors: Li J,Wu X,Chen Y,Zeng R,Zhao Y,Chang P,Wang D,Zhao Q,Deng Y,Li Y,Alam HB,Chong W

    更新日期:2016-12-01 00:00:00

  • Macelignan inhibits histamine release and inflammatory mediator production in activated rat basophilic leukemia mast cells.

    abstract::Type I allergy is characterized by the release of granule-associated mediators, lipid-derived substances, cytokines, and chemokines by activated mast cells. To evaluate the anti-allergic effects of macelignan isolated from Myristica fragrans Houtt., we determined its ability to inhibit calcium (Ca(2+)) influx, degranu...


    pub_type: 杂志文章


    authors: Han YS,Kim MS,Hwang JK

    更新日期:2012-10-01 00:00:00

  • Properties of concanavalin A-elicited granule exocytosis from human polymorphonuclear neutrophils.

    abstract::Exposure of human neutrophils to concanavalin A (Con A) resulted in a time- and concentration-dependent extracellular release of granule-associated lysozyme but not beta-glucuronidase or cytosolic lactate dehydrogenase. Maximum extrusion of lysozyme occurred 30 min after cell contact with Con A. The percent of total g...


    pub_type: 杂志文章


    authors: Smith RJ,Iden SS

    更新日期:1980-12-01 00:00:00

  • Chemotactic and immunoregulatory properties of bone cells are modulated by endotoxin-stimulated lymphocytes.

    abstract::In our study, we explored the bidirectional communication via soluble factors between bone cells and endotoxin-stimulated splenic lymphocytes in an in vitro coculture model that mimics the inflammatory environment. Both the ability of lymphocytes to affect differentiation and immune properties of bone cells, osteoblas...


    pub_type: 杂志文章


    authors: Cvija H,Kovacic N,Katavic V,Ivcevic S,Aguila HL,Marusic A,Grcevic D

    更新日期:2012-10-01 00:00:00

  • Do NSAIDs affect the progression of osteoarthritis?

    abstract::NSAIDs are widely used to alleviate the symptoms of OA. It remains controversial as to what effects these agents have on the progression of OA. In vitro studies showed several types of NSAIDs (e.g., sodium salicylate, indomethacin) inhibited the synthesis of cartilage matrix component, but some types of NSAIDs (e.g., ...


    pub_type: 杂志文章,评审


    authors: Ding C

    更新日期:2002-06-01 00:00:00

  • Partial Depletion of Regulatory T Cells Enhances Host Inflammatory Response Against Acute Pseudomonas aeruginosa Infection After Sepsis.

    abstract::Immune dysfunction contributes to secondary infection and worse outcomes in sepsis. Regulatory T cells (Tregs) have been implicated in sepsis-induced immunosuppression. Nevertheless, the role of Tregs in secondary infection after sepsis remains to be determined. In the present study, a two-hit model which mimics clini...


    pub_type: 杂志文章


    authors: Hu ZQ,Yao YM,Chen W,Bian JL,Zhao LJ,Chen LW,Hong GL,Lu ZQ,Zhao GJ

    更新日期:2018-10-01 00:00:00

  • Activation of the NLRP3 Inflammasome Is Associated with Valosin-Containing Protein Myopathy.

    abstract::Aberrant activation of the NOD-like receptor (NLR) family, pyrin domain-containing protein 3 (NLRP3) inflammasome, triggers a pathogenic inflammatory response in many inherited neurodegenerative disorders. Inflammation has recently been associated with valosin-containing protein (VCP)-associated diseases, caused by mi...


    pub_type: 杂志文章


    authors: Nalbandian A,Khan AA,Srivastava R,Llewellyn KJ,Tan B,Shukr N,Fazli Y,Kimonis VE,BenMohamed L

    更新日期:2017-02-01 00:00:00

  • Blockade of integrin VLA-4 prevents inflammation and matrix metalloproteinase expression in a murine model of accelerated collagen-induced arthritis.

    abstract::DBA/1LacJ mice were immunized with type II collagen and boosted with bacterial lipopolysaccharide (LPS) 17 days later to induce accelerated arthritis. Clinical signs of inflammation were observed as early as Day 20. Matrix metalloproteinases MMP-2, -3, -9, and -13, but not MMP-12, mRNA levels were increased on Day 24....


    pub_type: 杂志文章


    authors: Raychaudhuri A,Chou M,Weetall M,Jeng AY

    更新日期:2003-04-01 00:00:00

  • Analysis of the PKC-gamma-related immunocrossreactive region of a novel leukocyte protein gamma-rp.

    abstract::The newly discovered gamma-PKC-related-protein of human leukocytes (gamma-rp) crossreacts with a polyclonal antibody preparation originally designed to be specific for PKC-gamma (gammaMb-Ab). As this antibody is currently the only suitable probe for gamma-rp, we sought to characterize the binding of the two proteins. ...


    pub_type: 杂志文章


    authors: Smallwood JI,Malawista SE

    更新日期:1999-08-01 00:00:00

  • Impact of environmental factors on efficacy of glucocorticoids in Chinese population with systemic lupus erythematosus.

    abstract::Although glucocorticoids (GCs) are effective in inducing remission in systemic lupus erythematosus (SLE) patients, there is a significant variation in response to therapeutic GCs, and some patients do not achieve full remission. The aim of this study was to explore the impact of environmental factors on the efficacy o...


    pub_type: 杂志文章


    authors: Zou YF,Xu JH,Tao JH,Xu SQ,Liu S,Chen SY,Cai J,Lian L,Chen PL,Wang DG,Liu SX,Liang CM,Ye QL,Tian G,Wu M,Pan HF,Pan FM,Su H,Ye DQ

    更新日期:2013-12-01 00:00:00

  • Physio-pharmacological Investigations About the Anti-inflammatory and Antinociceptive Efficacy of (+)-Limonene Epoxide.

    abstract::D-limonene epoxidation generates (+)-limonene epoxide, an understudied compound in the pharmacologically point of view. Herein, we investigated the anti-inflammatory and antinociceptive potentialities of (+)-limonene epoxide and suggested a mechanism of action. The anti-inflammatory potential was analyzed using agents...


    pub_type: 杂志文章


    authors: de Almeida AA,Silva RO,Nicolau LA,de Brito TV,de Sousa DP,Barbosa AL,de Freitas RM,Lopes LD,Medeiros JR,Ferreira PM

    更新日期:2017-04-01 00:00:00

  • Dichloromethane fraction of Laminaria japonica ethanolic extract inhibits lipopolysaccharide-induced nitric oxide synthase and cyclooxygenase-2 expression in RAW 264.7 cells via NF-κB pathway.

    abstract::Strong anti-inflammatory activity has been found in Laminaria japonica dichloromethane fraction (LDF); however, the molecular mechanisms underlying its anti-inflammatory activity are not reported. Our results indicated that LDF inhibited LPS-induced nitric oxide and prostaglandin E(2) production in a dose-dependent ma...


    pub_type: 杂志文章


    authors: Lee JY,Lee MS,Choi JW,Shin TS,Woo HC,Kim HR

    更新日期:2012-10-01 00:00:00

  • Aurora kinase a regulates m1 macrophage polarization and plays a role in experimental autoimmune encephalomyelitis.

    abstract::Macrophage polarization is a dynamic and integral process of tissue inflammation and remodeling. Here we demonstrate an important role of Aurora kinase A in the regulation of inflammatory M1 macrophage polarization. We found that there was an elevated expression of Aurora-A in M1 macrophages and inhibition of Aurora-A...


    pub_type: 杂志文章


    authors: Ding L,Gu H,Gao X,Xiong S,Zheng B

    更新日期:2015-04-01 00:00:00

  • Cepharanthine attenuates lipopolysaccharide-induced mice mastitis by suppressing the NF-κB signaling pathway.

    abstract::Cepharanthine (CEP), a biscoclaurine alkaloid isolated from Stephania cepharantha Hayata, has been reported to have potent anti-inflammatory properties. However, the anti-inflammatory effects of CEP on a mouse model of lipopolysaccharide (LPS)-induced mastitis and its underlying molecular mechanisms remain to be eluci...


    pub_type: 杂志文章


    authors: Ershun Z,Yunhe F,Zhengkai W,Yongguo C,Naisheng Z,Zhengtao Y

    更新日期:2014-04-01 00:00:00