Characterization of synaptic vesicles and related neuronal features in nerve growth factor and ras oncogene differentiated PC12 cells.


:PC12 cells can differentiate into neuron-like cells after treatment with either nerve growth factor (NGF) or transduction with a retrovirus which expresses the K-ras oncogene. The concomitant treatment of NGF plus ras differentiates PC12 cells further than either agent alone with respect to neurite outgrowth, acetylcholinesterase levels, and most strikingly, the number of synaptic vesicle (SV) clusters. These SV clusters in PC12 cell neurites closely resemble those in the presynaptic terminals of neurons. Such SV clusters have not been described in cell lines previously. The SV clusters from all three differentiated groups (NGF, ras, and NGF plus ras) were similar in size, shape, and configuration, except that the ones in the doubly treated group occur in higher frequency and have more vesicles. The synaptic nature of these vesicle clusters was demonstrated by their regulated depletion after potassium stimulation. Furthermore, these vesicle clusters stained positively for two SV-associated proteins, synapsin I and synaptophysin, by EM immunocytochemistry (ICC). Such SV clusters in a cell line are very useful for characterizing the regulated release of SVs and the distribution of SV-related antigens in intact cells. Analysis by SDS-gel electrophoresis and immunoblotting indicated that synapsin I levels are higher in all three differentiated groups compared to untreated cells; whereas synaptophysin levels are lower in cells exposed to NGF alone or with NGF and ras double treatment. Possible convergence and/or divergence on the mechanisms of NGF and ras differentiation in PC12 cells are discussed.


J Neurosci Res


Tao-Cheng JH,Dosemeci A,Bressler JP,Brightman MW,Simpson DL




Has Abstract


1995-10-15 00:00:00












  • Human fetal cortical and striatal neural stem cells generate region-specific neurons in vitro and differentiate extensively to neurons after intrastriatal transplantation in neonatal rats.

    abstract::Human fetal brain is a potential source of neural stem cells (NSCs) for cell replacement therapy in neurodegenerative diseases. We explored whether NSCs isolated from cortex and striatum of human fetuses, aged 6-9 weeks post-conception, maintain their regional identity and differentiate into specific neuron types in c...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: Kallur T,Darsalia V,Lindvall O,Kokaia Z

    更新日期:2006-12-01 00:00:00

  • Pentylenetetrazol seizures increase pro-nerve growth factor-like immunoreactivity in the reticular thalamic nucleus and nerve growth factor mRNA in the dentate gyrus.

    abstract::Neurotrophins may have a neuroprotective role and are probably involved in the control of axonal sprouting and synaptic plasticity. An antibody raised against a pro-sequence of nerve growth factor (NGF) was tested. In control undisturbed rats, a strong immunoreactivity was detected in scattered cells in and around the...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: Humpel C,Ebendal T,Cao Y,Olson L

    更新日期:1993-07-01 00:00:00

  • NMDA receptor-mediated cGMP synthesis in primary cultures of mouse cerebellar granule cells appears to involve neuron-astrocyte communication with NO operating as the intercellular messenger.

    abstract::The possibility that neuron-astrocyte communication may be responsible for glutamate (Glu)-stimulated cGMP formation even in relatively homogeneous primary cultures of mouse cerebellar granule cells (7 days in vitro) was investigated. Pharmacological analysis using selective excitatory amino acid (EAA) receptor antago...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: Malcolm C,Grieve A,Ritchie L,Schousboe A,Griffiths R

    更新日期:1996-07-15 00:00:00

  • Regulation of the Na+-dependent high affinity glutamate/aspartate transporter in cultured Bergmann glia by phorbol esters.

    abstract::The effects of phorbol 12-tetradecanoyl-13-acetate (TPA) and dibutyryl cAMP on the glutamate transport present in chick Bergmann glial cell (BGC) cultures were examined. TPA produced a significant decrease in [3H]-D-aspartate uptake, while dibutyryl cAMP treatment elicited a slight reduction in the transport. This eff...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: González MI,Ortega A

    更新日期:1997-11-15 00:00:00

  • Intracellular pH changes during oligodendrocyte differentiation in primary culture.

    abstract::We have studied the characteristics of pH(i) regulation at different stages of rat oligodendrocyte differentiation in primary culture. pH(i) was measured at 37 degrees C using the pH-sensitive fluorescent probe BCECF. In immature oligodendrocyte progenitor (OLP), three distinct ionic mechanisms were involved in pH(i) ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: Boussouf A,Gaillard S

    更新日期:2000-03-15 00:00:00

  • Studies on the effects of chronic ethanol ingestion on the properties of rat brain ribosomes.

    abstract::Previous observations have demonstrated decreased in vivo and in vitro protein synthesis by brain ribosomal systems following long-term ethanol ingestion. For further investigation of the properties of brain ribosomes, the 40S and 60S ribosomal subunits were successfully isolated from control and chronic 10% ethanol-d...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: Tewari S,Murray S,Noble EP

    更新日期:1978-01-01 00:00:00

  • Metabolic approach of absence seizures in a genetic model of absence epilepsy, the GAERS: study of the leucine-glutamate cycle.

    abstract::We suggest that a dysregulation of energy metabolism in the brain of genetic absence epilepsy rats from Strasbourg (GAERS) could create a specific cerebral environment that would favor the expression of spike-and-wave discharges (SWD) in the thalamocortical loop, largely dependent on glutamatergic and gamma-aminobutyr...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: Dufour F,Nalecz KA,Nalecz MJ,Nehlig A

    更新日期:2001-12-01 00:00:00

  • Regulatory factor X4 variant 3: a transcription factor involved in brain development and disease.

    abstract::Regulatory factor X4 variant 3 (RFX4_v3) is a recently identified transcription factor specifically expressed in the brain. Gene disruption in mice demonstrated that interruption of a single allele (heterozygous, +/-) prevented formation of the subcommissural organ (SCO), resulting in congenital hydrocephalus, whereas...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章,评审


    authors: Zhang D,Zeldin DC,Blackshear PJ

    更新日期:2007-12-01 00:00:00

  • A novel biosensor with high signal-to-noise ratio for real-time measurement of dopamine levels in vivo.

    abstract::Fast-scan cyclic voltammetry (FSCV) is an established method for measuring dopamine (DA) levels in the brain in real time. However, it is difficult to discriminate DA from other monoamines such as serotonin (5-hydroxytryptamine, 5-HT) and norepinephrine (NE). We report a novel DA-specific biosensor consisting of a car...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: Ishida A,Imamura A,Ueda Y,Shimizu T,Marumoto R,Jung CG,Hida H

    更新日期:2018-05-01 00:00:00

  • Functional role of the potassium-induced stimulation of oxygen uptake in brain slices studied with cesium as a probe.

    abstract::The potassium-induced stimulation of oxygen consumption in brain slices has a threshold value of 15-20 mM potassium, and it reaches its maximum at 35-50 mM. Although this phenomenon now has been known for almost 50 years, its physiological role remains undetermined. One reason for this may be that the high concentrati...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: Hertz L,Kjeldsen CS

    更新日期:1985-01-01 00:00:00

  • Distribution of neuronal intermediate filament proteins in the developing mouse olfactory system.

    abstract::The distribution of neuronal intermediate filament proteins in the developing mouse olfactory bulb and olfactory epithelium was characterized by immunocytochemical approach. Antibodies against alpha-internexin, neurofilament triplet proteins (NFTPs; NF-L, NF-M, and NF-H) and peripherin were used to determine their exp...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: Chien CL,Lee TH,Lu KS

    更新日期:1998-11-01 00:00:00

  • Chronic odorant exposure upregulates acquisition of functional properties in cultured embryonic chick olfactory sensory neurons.

    abstract::Neuronal development and differentiation is modulated by activity-dependent mechanisms that stimulate endogenous neurogenesis and differentiation to promote adaptive survival of the organism. Studies on bird odor imprinting have shown how sensory stimuli or environmental influences can affect neonatal behavior, presum...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: O'Neill G,Musto C,Gomez G

    更新日期:2017-05-01 00:00:00

  • Integrin-type signaling has a distinct influence on NMDA-induced cytoskeletal disassembly.

    abstract::Adhesion responses triggered by integrin-class matrix receptors have been implicated in the synaptic reorganization events necessary for certain types of neuronal plasticity. Hippocampal slice cultures were used to test whether the related structural transformations elicited by NMDA receptor stimulation are regulated ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: Bahr BA

    更新日期:2000-03-15 00:00:00

  • Characterization of neurotrophin receptors by affinity crosslinking.

    abstract::Neurotrophic factors regulate the developmental survival and differentiation of specific neuronal populations. Brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) are members of the nerve growth factor (NGF) protein family, also known as the neurotrophins. Insights into the different roles of neurotroph...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: Escandón E,Burton LE,Szönyi E,Nikolics K

    更新日期:1993-04-15 00:00:00

  • Reactive astrogliosis induces astrocytic differentiation of adult neural stem/progenitor cells in vitro.

    abstract::Neural stem cells reside in defined areas of the adult mammalian brain, including the dentate gyrus of the hippocampus. Rat neural stem/progenitor cells (NSPCs) isolated from this region retain their multipotency in vitro and in vivo after grafting into the adult brain. Recent studies have shown that endogenous or gra...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: Faijerson J,Tinsley RB,Apricó K,Thorsell A,Nodin C,Nilsson M,Blomstrand F,Eriksson PS

    更新日期:2006-11-15 00:00:00

  • Expression analysis of the PMP22 gene in glioma and osteogenic sarcoma cell lines.

    abstract::Previously we reported the amplification of the peripheral myelin protein 22 (PMP22) gene in cell lines of human osteogenic and glioma tumors. PMP22 normally is expressed at high levels in Schwann cells of the peripheral nervous system and is suggested to function as a structural protein of the myelin sheath. One of t...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: Hühne K,Park O,Liehr T,Rautenstrauss B

    更新日期:1999-12-01 00:00:00

  • NO-induced downregulation of HSP10 and HSP60 expression in the postischemic brain.

    abstract::Heat shock protein 60 (HSP60) and HSP10 are mitochondrial chaperonin proteins and are responsible for the folding of newly imported proteins and 13 polypeptides encoded by mitochondrial DNA. The expressions of HSP60 and HSP10 are regulated simultaneously because these genes are localized head to head on chromosome, se...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: Kim SW,Lee JK

    更新日期:2007-05-01 00:00:00

  • Increase of ryanodine receptors by dopamine D1 receptors is negatively regulated by γ-aminobutyric acid type B receptors in primary cultures of mouse cerebral cortical neurons.

    abstract::Although upregulation of ryanodine receptor (RyR)-1 and -2 is mediated through the activation of dopamine D1 receptors (D1DRs) in the development of psychostimulant-induced place preference, little is known about how such increased expressions of RyRs are negatively regulated. This study investigated negative regulato...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: Kurokawa K,Mizuno K,Ohkuma S

    更新日期:2012-08-01 00:00:00

  • Time-to-contact perception in the brain.

    abstract::Time-to-contact (TTC) perception refers to the ability of an observer to estimate the remaining time before an object reaches a point in the environment, and is of crucial importance in daily life. Noninvasive correlational approaches have identified several brain areas sensitive to TTC information. Here we report the...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: Baurès R,Fourteau M,Thébault S,Gazard C,Pasquio L,Meneghini G,Perrin J,Rosito M,Durand JB,Roux FE

    更新日期:2021-02-01 00:00:00

  • Molecules affecting myelin stability: a novel hypothesis regarding the pathogenesis of multiple sclerosis.

    abstract::In this Mini-Review we present a new hypothesis in support of the neurodegenerative theory as a mechanism for the pathogenesis of multiple sclerosis (MS). The pathogenesis of MS results from changes in two distinct CNS compartments. These are the "myelin" and "nonmyelin" compartments. The myelin compartment is where p...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章,评审


    authors: Mastronardi FG,Moscarello MA

    更新日期:2005-05-01 00:00:00

  • Attenuation of malonate toxicity in primary mesencephalic cultures using the GABA transport blocker, NO-711.

    abstract::Cultured rat mesencephalic neurons were used to assess the effects of gamma-aminobutyric acid (GABA) transport blockers on toxicity caused by malonate, a reversible, competitive inhibitor of succinate dehydrogenase. Previous studies utilizing an ex vivo chick retinal preparation have shown that GABA release and cell s...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: Stokes AH,Bernard LP,Nicklas WJ,Zeevalk GD

    更新日期:2001-04-01 00:00:00

  • Restoration of thalamo-cortical connectivity after brain injury: recovery of consciousness, complex behavior, or passage of time?

    abstract::In 2000, a landmark case report described the concurrent restoration of consciousness and thalamo-frontal connectivity after severe brain injury (Laureys et al., ). Being a single case however, this study could not disambiguate whether the result was specific to the restoration of consciousness per se as opposed to th...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: Crone JS,Bio BJ,Vespa PM,Lutkenhoff ES,Monti MM

    更新日期:2018-04-01 00:00:00

  • Extracellular signal-related kinase 2/specificity protein 1/specificity protein 3/repressor element-1 silencing transcription factor pathway is involved in Aroclor 1254-induced toxicity in SH-SY5Y neuronal cells.

    abstract::Polychlorinated biphenyls (PCBs) cause a wide spectrum of toxic effects in the brain through undefined mechanisms. Exposure to the PCB mixture Aroclor-1254 (A1254) increases the repressor element-1 silencing transcription factor (REST) expression, leading to neuronal death. This study sought to understand the sequence...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: Formisano L,Guida N,Laudati G,Boscia F,Esposito A,Secondo A,Di Renzo G,Canzoniero LM

    更新日期:2015-01-01 00:00:00

  • Resolving the contributions of anaesthesia, surgery, and nerve injury on brain derived neurotrophic factor expression in the medial prefrontal cortex of male rats in the CCI model of neuropathic pain.

    abstract::The medial prefrontal cortex (mPFC) is critical for selecting and shaping complex behavioral responses. In rodent models of neuropathic pain there is evidence for both structural and functional changes in the mPFC. Brain derived neurotrophic factor (BDNF) plays a critical role in the normal functioning of the mPFC. It...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: Kang JWM,Keay KA,Mor D

    更新日期:2017-12-01 00:00:00

  • Expression of insulin-like growth factor-binding protein messenger RNAs in developing rat oligodendrocytes and astrocytes.

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    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: Mewar R,McMorris FA

    更新日期:1997-12-01 00:00:00

  • Phosphorylative neuromodulation of the regulatory subunit of cyclic AMP-dependent protein kinase type II in skeletal muscle.

    abstract::The phosphorylative neuromodulation of the regulatory subunit of protein kinase type II (R-II) in cytosolic fractions from denervated and sham-operated, contralateral soleus muscles of the rat was evaluated. The denervation-induced increase in the 32P-phosphorylation of R-II is not related to an increased dephosphoryl...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: McLane JA,Squinto SP,Yeoh HC,Held IR

    更新日期:1985-01-01 00:00:00

  • Cannabinoid receptor agonist-stimulated [35S]guanosine triphosphate gammaS binding in the brain of C57BL/6 and DBA/2 mice.

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    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: Basavarajappa BS,Hungund BL

    更新日期:2001-05-15 00:00:00

  • Fatty acid and long-chain base composition of gangliosides isolated from adult human brain.

    abstract::A series of major and minor ganglioside species were isolated from a single human adult brain and analyzed for their lipophilic composition. Hematosides, GM3 and GD3, each showing double bands on thin-layer chromatograms, were separated into the upper band and lower band fractions to be analyzed for the heterogeneity ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: Ando S,Yu RK

    更新日期:1984-01-01 00:00:00

  • The lactate receptor, G-protein-coupled receptor 81/hydroxycarboxylic acid receptor 1: Expression and action in brain.

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    journal_title:Journal of neuroscience research

    pub_type: 杂志文章,评审


    authors: Morland C,Lauritzen KH,Puchades M,Holm-Hansen S,Andersson K,Gjedde A,Attramadal H,Storm-Mathisen J,Bergersen LH

    更新日期:2015-07-01 00:00:00

  • Interaction between protein kinase C and protein kinase A can modulate transmitter release at the rat neuromuscular synapse.

    abstract::We used intracellular recording to investigate the functional interaction between protein kinase C (PKC) and protein kinase A (PKA) signal transduction cascades in the control of transmitter release in the neuromuscular synapses from adult rats. Our results indicate that: 1) PKA and PKC are independently involved in a...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: Santafé MM,Garcia N,Lanuza MA,Tomàs M,Tomàs J

    更新日期:2009-02-15 00:00:00