Establishment and characterization of a mouse Schwann cell line which produces myelin in vivo.

Abstract:

:A Schwann cell line (MSC 80) was established from purified mouse Schwann cell cultures using large doses of serum. MSC 80 cell line is an aneuploid cell line which has a doubling time of 17 hr and has been maintained through more than 110 passages. Most of MSC 80 cells are of bipolar or stellate (3-5 processes) shape. A few others are irregular in shape, gigantic, and multinucleated. All MSC 80 cells express antigens of myelin-forming Schwann cells such as S-100, 224/58, laminin, and other glycoproteins of the extracellular matrix. However, they also express the non-myelin-forming Schwann cell antigen GFAP. By time-lapse cinematography, MSC 80 cells exhibit the Schwann cell characteristic rhythmical undulations. When induced to form aggregates in agar, they form intercellular junctions and basement membrane-like structures. In addition, after transplantation in or at a distance from a lysolecithin induced lesion, MSC 80 cells form myelin around the host demyelinated axons. MSC 80 cells thus express, when isolated in vitro, some of the normal myelin-forming Schwann cell phenotype. In addition, they present the major advantage of forming myelin when associated with axons in vivo.

journal_name

J Neurosci Res

authors

Boutry JM,Hauw JJ,Gansmüller A,Di-Bert N,Pouchelet M,Baron-Van Evercooren A

doi

10.1002/jnr.490320103

keywords:

subject

Has Abstract

pub_date

1992-05-01 00:00:00

pages

15-26

issue

1

eissn

0360-4012

issn

1097-4547

journal_volume

32

pub_type

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