Abstract:
:A Schwann cell line (MSC 80) was established from purified mouse Schwann cell cultures using large doses of serum. MSC 80 cell line is an aneuploid cell line which has a doubling time of 17 hr and has been maintained through more than 110 passages. Most of MSC 80 cells are of bipolar or stellate (3-5 processes) shape. A few others are irregular in shape, gigantic, and multinucleated. All MSC 80 cells express antigens of myelin-forming Schwann cells such as S-100, 224/58, laminin, and other glycoproteins of the extracellular matrix. However, they also express the non-myelin-forming Schwann cell antigen GFAP. By time-lapse cinematography, MSC 80 cells exhibit the Schwann cell characteristic rhythmical undulations. When induced to form aggregates in agar, they form intercellular junctions and basement membrane-like structures. In addition, after transplantation in or at a distance from a lysolecithin induced lesion, MSC 80 cells form myelin around the host demyelinated axons. MSC 80 cells thus express, when isolated in vitro, some of the normal myelin-forming Schwann cell phenotype. In addition, they present the major advantage of forming myelin when associated with axons in vivo.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Boutry JM,Hauw JJ,Gansmüller A,Di-Bert N,Pouchelet M,Baron-Van Evercooren Adoi
10.1002/jnr.490320103keywords:
subject
Has Abstractpub_date
1992-05-01 00:00:00pages
15-26issue
1eissn
0360-4012issn
1097-4547journal_volume
32pub_type
杂志文章abstract::Although proteins belonging to the sHSP superfamily are diverse in sequence and size, most share characteristic features, including 1) a small molecular mass of 12-43 kDa, 2) a conserved alpha-crystallin domain of 80-100 residues, 3) formation of large oligomers, 4) a dynamic quaternary structure, and 5) induction by ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.21682
更新日期:2008-08-01 00:00:00
abstract::Macroautophagy is an evolutionally conserved membrane trafficking pathway that delivers intracellular materials to lysosomes for degradation and recycling. Rab7, as a member of the Rab GTPase superfamily, has a unique role in the regulation of macroautophagy, especially in modulating autophagy flux. The functional sta...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.24034
更新日期:2017-10-01 00:00:00
abstract::The extension of filopodia from growth cones of regenerating neurites from rat superior cervical ganglion neurons in tissue culture was studied. Cultures were grown on a thin layer of fibrous collagen and maintained in a medium containing serum and nerve growth factor. Time-lapse cinematography and computer-assisted m...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490130111
更新日期:1985-01-01 00:00:00
abstract::Neuronal pathology of the brain with Alzheimer's disease (AD) is characterized by numerous depositions of amyloid-beta peptides (Abeta). Abeta binding to the 75-kDa neurotrophin receptor (p75NTR) causes neuronal cell death. Here we report that Abeta causes cell death in neuronal hybrid cells transfected with p75NTR, b...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10703
更新日期:2003-09-01 00:00:00
abstract::Using [3H]-dopamine (DA) as the ligand of choice, we have shown that rat lymphocytes possess DA binding sites. The binding of [3H]-DA was specific, saturable, and of high affinity. The specific binding in membranes obtained from Con A-activated lymphoblasts was greater than the binding in respective unactivated lympho...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490180112
更新日期:1987-01-01 00:00:00
abstract::Evidence indicates that the ubiquitin-proteasome system and the endoplasmic retculum (ER) quality-control system work in concert to ensure that proteins are correctly folded in the ER and that misfolded proteins are retrotransported to the cytosol for degradation by proteasomes. Dysfunction of either system results in...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23413
更新日期:2014-10-01 00:00:00
abstract::Localization of acetylcholine receptors (AChRs) to the postsynaptic region of muscle is mediated in part by transcriptional mechanisms, because the genes encoding AChR subunits are transcribed selectively in synaptic myofiber nuclei. Neuregulin-1 (NRG-1) is a synaptic signal and induces transcription of AChRs in muscl...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21563
更新日期:2008-04-01 00:00:00
abstract::The c-Jun N-terminal kinase (JNK) is induced by cerebral ischemia and injurious blood components acutely after subarachnoid hemorrhage (SAH). We hypothesized that inhibition of JNK will prevent damage to the neurovascular unit in the early brain injury period after SAH. Ninety-nine male SD rats (300-350 g) were random...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21281
更新日期:2007-05-15 00:00:00
abstract::We have proposed that lactate is a "volume transmitter" in the brain and underpinned this by showing that the lactate receptor, G-protein-coupled receptor 81 (GPR81, also known as HCA1 or HCAR1), which promotes lipid storage in adipocytes, is also active in the mammalian brain. This includes the cerebral neocortex and...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.23593
更新日期:2015-07-01 00:00:00
abstract::The activity levels of pyruvate dehydrogenase, enzymes of the citric acid cycle, aspartate and alanine aminotransferases, and NADP+-isocitrate dehydrogenase were determined in the cerebral cortex, cerebellum, brain stem, corpus striatum, hippocampus, and midbrain regions of normal rats and rats injected with acute and...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490140407
更新日期:1985-01-01 00:00:00
abstract::We have determined the localization of Cav1.2 (L-Type) Ca2+ channels in the cells and nerve fibers in molars of normal or injured rats. We observed high levels of immunostaining of L-type Ca2+ channels in odontoblast cell bodies and their processes, in fibroblast cell bodies and in Schwann cells. Many Cav1.2-containin...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10863
更新日期:2004-02-01 00:00:00
abstract::Programmed cell death involving gene regulation and de novo protein synthesis is a major component of both normal development and a number of disease conditions. Hence, knowledge of its mechanisms, especially transcription factors, that regulate expression of the genes involved in neurodegenerative disorders is of gre...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21469
更新日期:2008-01-01 00:00:00
abstract::Studies using Aplysia californica have demonstrated that transcription after nerve injury occurs during a rapid, transient first phase and a delayed, prolonged second phase. Although the second phase is especially important for regeneration, the mRNAs produced during this phase have not been identified. We characteriz...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20645
更新日期:2005-11-15 00:00:00
abstract::Dissociated neurons from the neural tube containing the trigeminal motor nucleus from early chick embryos were cultured on laminin or collagen-polyornithine substrates, with and without nerve growth factor (NGF). Control cultures were grown in similar conditions with cytochrome-C. It was found that neuronal survival w...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490220404
更新日期:1989-04-01 00:00:00
abstract::The actions of endogenous opioids and nociceptin/orphanin FQ are mediated by four homologous G protein-coupled receptors that constitute the opioid receptor family. However, little is known about opioid systems in cyclostomes (living jawless fish) and how opioid systems might have evolved from invertebrates. Here, we ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.24682
更新日期:2020-08-23 00:00:00
abstract::Cell death has been described as either apoptotic, in which the cell actively participates, or necrotic, in which the cell is felt to be passive. The proto-oncogene bcl-2 has been shown to inhibit apoptosis in some hematopoietic and neural cells, by an unknown mechanism. We demonstrate that bcl-2 inhibits the necrosis...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490400216
更新日期:1995-02-01 00:00:00
abstract::Fibroblast growth factors (FGFs) are known to be synthesized in the central nervous system (CNS) and to act on CNS cells in vitro, but less is known about their synthesis, expression, and role in vivo. In this work, using specific anti-acidic fibroblast growth factor (aFGF) antibodies, we have shown for the first time...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490290417
更新日期:1991-08-01 00:00:00
abstract::The limited availability and potential to culture primary human brain cells means that there is still a need for cell lines that reliably model human neurons and glial cells. The human-derived NTera2/D1 (NT2) cell line is a promising tool from which both neuronal (NT2N) and astrocytic (NT2A) cells can be derived in vi...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22692
更新日期:2011-10-01 00:00:00
abstract::In a number of pathological states of the brain, the activation of the inducible nitric oxide synthase (iNOS) plays a major role. Interleukin (IL)-1beta is believed to be an essential factor in the induction of iNOS. However, little is known about the cascade of events culminating in iNOS expression in vivo. To identi...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00
abstract::Increased serum level of homocysteine, a sulfur-containing amino acid, is considered a risk factor in vascular disorders and in dementias. The effect of homocysteine and metabolically related compounds on brain production of kynurenic acid (KYNA), an endogenous antagonist of glutamate ionotropic receptors, was studied...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20359
更新日期:2005-02-01 00:00:00
abstract::The effects of cations on the spontaneous and potassium-stimulated taurine release from mouse cerebral cortex slices were assessed with an emphasis on the as yet unestablished calcium dependence of the stimulated release. Spontaneous and stimulated GABA release was analyzed for comparison. A depolarizing concentration...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490170316
更新日期:1987-01-01 00:00:00
abstract::Previous studies have demonstrated variability in the phenotype of rat C6 glioma cells. In the present study, we compared morphology, growth rate, and beta-adrenergic regulation of gene expression in early (P39-47) and late (P55-90) passage C6 cells. Morphological changes were observed in five independently derived, l...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490330213
更新日期:1992-10-01 00:00:00
abstract::The role of insulin-like growth factor-1 (IGF-1) in amyotrophic lateral sclerosis (ALS) and its mechanism of action are important from both pathogenic and therapeutic points of view. The present study investigated the changes of IGF-1Rbeta and the key intracellular downstream protein insulin receptor substrate-1 (IRS-...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20668
更新日期:2005-11-15 00:00:00
abstract::Toll-like receptors (TLRs) play an essential role in initiating intracellular type I interferon (IFN)-mediated innate immunity against viral infections. We examined whether human neuronal cells (primary human neurons, NT2-N and CHP-212 cells) express TLRs and mount type I IFN-mediated innate immunity against herpes si...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22110
更新日期:2009-10-01 00:00:00
abstract::The purpose of this study was to determine if calpain-induced proteolysis was associated with retinal degeneration or dysfunction in the rat acute ocular hypertensive model. Acute glaucoma was produced by elevation of IOP to 120 mm Hg for 1 hr. Retinal degeneration was evaluated by H&E staining and apoptosis was deter...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20827
更新日期:2006-05-15 00:00:00
abstract::A link between aluminum (Al) exposure and age-related neurological disorders has long been proposed. Although the exact mechanism by which the metal may influence disease processes is unknown, there is evidence that exposure to Al causes an increase in both oxidative stress and inflammatory events. These processes hav...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10877
更新日期:2004-02-15 00:00:00
abstract::Comparisons were made of whole cell voltage clamp recordings from cultures of normal Schwann cells (SC) from three human subjects and from three neurofibrosarcoma cell lines. The whole cell K+ (K) currents of normal and tumor cells could be divided into three types based on voltage activation range, pharmacology, and ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19981115)54:4<495::AID-JNR
更新日期:1998-11-15 00:00:00
abstract::The activation of cellular inflammatory response is tightly linked to induced production of reactive oxygen species (ROS) and nitric oxide (NO), which in turn have been identified as important regulators of cellular iron metabolism. In the present study, we have used the microglia cell line BV-2 and the neuroblastoma ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21005
更新日期:2006-10-01 00:00:00
abstract::The tissue- and cell-specific expression of three neutral glycosphingolipids, gangliotetraosylceramide (GA1), gangliopentaosylceramide (GalNAc-GA1), and the novel 3-O-acetyl-sphingosine-series glycolipid (FMC-5), were examined with monospecific polyclonal antibodies. Immunohistochemical studies of rodent brain cross-s...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21419
更新日期:2007-10-01 00:00:00
abstract::Astrocytes are glial cells that are distributed throughout the central nervous system in an arrangement optimal for chemical and physical interaction with neuronal synapses and brain blood supply vessels. Neurotransmission modulates astrocytic excitability by activating an array of cell surface receptors and transport...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.24075
更新日期:2017-12-01 00:00:00