Abstract：
:The analysis of proteins in damaged myelin is crucial to clarify the mechanisms of dysmyelination and demyelination. In the present study, proteomic analysis of myelin using a modified two-dimensional electrophoresis (2-DE) method was carried out to obtain a better understanding of myelin biology. Although standard 2-DE (immobilized pH gradient isoelectric focusing/sodium dodecyl sulfate-polyacrylamide gel electrophoresis; IPG/SDS-PAGE) methods of analysis provide high resolutions of soluble proteins with isoelectric focusing points in the pH range of 4-8, major myelin components include highly basic proteins are compacted at the basic edge of the 2-DE gels and are not sufficiently separated for satisfactory analysis. In an attempt to improve the separation of these proteins, an alternative 2-DE method using the cationic detergents was applied. In part 1 of this study, we describe technical aspects of conditioning 2-DE using cationic detergent. In the accompanying paper (part 2), practical 2-DE analysis using cationic detergents is described to identify proteins in the purified CNS myelin fraction. We carried out benzyldimethyl-n-hexadecylammonium chloride (16-BAC)/SDS-PAGE 2-DE and tested 2-DE with four other cationic detergents. We found that 16-BAC was the most effective agent for separation of myelin proteins and that hexadecyltrimethylammonium bromide (cetyltrimethylammonium bromide; CTAB) was the most effective agent for solubilization of myelin proteins. The combination of 16-BAC/SDS-PAGE and CTAB/SDS-PAGE is a powerful tool for the analysis of myelin proteins, including highly basic, high-MW (MW > 100K), and integral membrane proteins.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Yamaguchi Y,Miyagi Y,Baba Hdoi
10.1002/jnr.21547subject
Has Abstractpub_date
2008-03-01 00:00:00pages
755-65issue
4eissn
0360-4012issn
1097-4547journal_volume
86pub_type
杂志文章abstract：:The effects of bilateral lesions of the ventral noradrenergic bundle (VNA) were studied in male rats. In contrast to data reported by others, hyperphagia and obesity were not observed following VNA lesions. Indeed, except for a depression during the first three postoperative days, food intake (FI) of the VNA lesioned ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490020305
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
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abstract：:The normal innervation of structures in mouse foot pads was investigated with immunohistochemistry and confocal microscopy. Nerves were visualized by incubating Zamboni fixed, thick, frozen sections with antibodies to protein gene product 9.5 (PGP 9.5), vasoactive intestinal peptide, substance P, calcitonin gene-relat...
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doi：10.1002/jnr.490410113
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490020514
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pub_type： 杂志文章,评审
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490120408
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.23702
更新日期：2016-06-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490130111
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.1084
更新日期：2001-05-15 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.23025
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20780
更新日期：2006-04-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(sici)1097-4547(19970801)49:3<389::aid-jnr
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journal_title：Journal of neuroscience research
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doi：10.1002/jnr.490300222
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journal_title：Journal of neuroscience research
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doi：10.1002/jnr.490340314
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journal_title：Journal of neuroscience research
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19960501)44:3<216::AID-JNR
更新日期：1996-05-01 00:00:00
abstract：:Experiments were conducted with the aid of intracellular microelectrodes to study physiological properties of neuropile glial cells in the central nervous system of the medicinal leech. The results showed significant contributions of both K+ and Cl- ions to the membrane potential. The transmitter substance 5-hydroxytr...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490070108
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abstract：:The most visible and, until very recently, the only hypothesis regarding the involvement of microglial cells in Alzheimer's disease (AD) pathogenesis is centered around the notion that activated microglia are neurotoxin-producing immune effector cells actively involved in causing the neurodegeneration that is the caus...
journal_title：Journal of neuroscience research
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doi：10.1002/jnr.20093
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