Abstract:
:Secretogranin II (chromogranin C), originally described as tyrosine sulfated protein of the anterior pituitary, is present in large dense core vesicles of several endocrine cells and neurons. We raised antisera in rabbits to conjugates of two synthetic peptides (bovine secretogranin 133-151 and rat secretogranin 154-186) flanked in the primary structure of secretogranin II by pairs of basic residues and used them to investigate the proteolytic processing of this protein by immunoblotting and a newly developed radioimmunoassay. The sensitivity of this assay was 30 fmol for secretogranin 154-186 and 60 fmol for secretogranin 133-151. The highest degree of processing of secretogranin II (> 90%) occurs in brain. One of the peptides (secretogranin 133-151) is not generated to any significant extent. The other peptide, secretogranin 154-186, however, is formed in vivo, and in brain the free peptide apparently represents the predominant form. The highest concentrations of secretogranin 154-186 are found in the hypothalamus, two- to six-fold lower levels are present in the hippocampus, caudate nucleus, thalamus and brainstem. These concentrations are comparable to those of established neuropeptides. In order to indicate the special relevance of secretogranin II and of this peptide for brain we have named this peptide secretoneurin. The newly developed radioimmunoassay for this peptide will be a useful tool to establish its physiologic role in brain.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Kirchmair R,Hogue-Angeletti R,Gutierrez J,Fischer-Colbrie R,Winkler Hdoi
10.1016/0306-4522(93)90200-ysubject
Has Abstractpub_date
1993-03-01 00:00:00pages
359-65issue
2eissn
0306-4522issn
1873-7544pii
0306-4522(93)90200-Yjournal_volume
53pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract::Sucrose gap techniques recorded dorsal root potentials evoked by supramaximal dorsal root stimulation in in vitro, hemisected frog spinal cords. In 0 mM Mg2+ large (mean 13.0 mV), long lasting (mean 8.1 s) dorsal root potentials were recorded which consisted of two components: (1) an early component sensitive to picro...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(91)90200-8
更新日期:1991-01-01 00:00:00
abstract::Axonal growth requires insertion of newly synthesized membrane components into the plasmalemma. Imbalance between exocytotic membrane addition and endocytic retrieval at specific axonal sites may lead to the bulk plasma membrane flow along the axon and, thus, contribute to the renewal of plasma membrane components. By...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(00)00022-1
更新日期:2000-01-01 00:00:00
abstract::The present studies aimed to determine whether estradiol (E(2)) modulates the stimulation of cocaine- and amphetamine-regulated transcript (CART) peptide in the mesolimbic and nigrostriatal dopaminergic systems. I.c.v. administration of the CART peptide (55-102, 1 microg/3 microl) increased dopamine turnover (3,4-dihy...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2008.01.086
更新日期:2008-07-17 00:00:00
abstract::Semi-quantitative immunocytochemistry was used to investigate the levels of cyclase response element-binding protein, phosphorylated cyclase response element-binding protein, Fos and Fos-related antigen immunoreactivity in the striatum of rats after acute or repeated amphetamine administration. Rats were perfused 20 m...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(95)00274-m
更新日期:1995-11-01 00:00:00
abstract::Using arsenazo III as an intracellular indicator to monitor the calcium transients elicited by voltage-clamp depolarizing pulse, the effect of prolonged in vitro lithium treatment on excitation--contraction coupling in frog twitch muscle fibres was investigated. Incubation in 10 mM Li+ Ringer's solution for 2 days cau...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(90)90240-5
更新日期:1990-01-01 00:00:00
abstract::Perceiving the sensory consequences of our actions with a delay alters the interpretation of these afferent signals and impacts motor learning. For reaching movements, delayed visual feedback of hand position reduces the rate and extent of visuomotor adaptation, but substantial adaptation still occurs. Moreover, the d...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2020.07.041
更新日期:2020-10-15 00:00:00
abstract::Although numerous studies have demonstrated the neurotrophic capacity of gp130 cytokines, it remains unclear whether endogenously expressed cytokines actually function in a direct neuromodulatory manner. Therefore, using the lithium-pilocarpine status epilepticus model, we performed a detailed in situ hybridization ti...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(03)00593-1
更新日期:2003-01-01 00:00:00
abstract::Transplantation of embryonic dopaminergic neurons is an experimental therapy for Parkinson's disease, but limited tissue availability and suboptimal survival of grafted dopaminergic neurons impede more widespread clinical application. Glial cell line-derived neurotrophic factor (GDNF) and neurotrophin-4/5 (NT-4/5) exe...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(01)00418-3
更新日期:2001-01-01 00:00:00
abstract::Obesity may result from dysfunction of the reward system, especially in the nucleus accumbens (Acb). Based on this hypothesis, many researchers have tested the effect of high-frequency stimulation (HFS) of the Acb shell (Acb-Sh) and/or core (Acb-Co) on ingestive behaviors, but few studies have explored the possible me...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.06.006
更新日期:2015-08-20 00:00:00
abstract::The role of the cerebral cortex in maintaining human standing balance remains unclear. Beta corticomuscular coherence (CMC) provides a measure of communication between the sensory-motor cortex and muscle, but past literature has not demonstrated significant beta CMC during human stance. This study evaluated the effect...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.04.009
更新日期:2015-07-09 00:00:00
abstract::The fact that neurobiological research is reliant upon laboratory-reared rodents is well known. The following paper discusses this topic broadly, but also aims to highlight other species used in the study of the nervous system and the evolution of animal species usage from the end of World War II through recent invest...
journal_title:Neuroscience
pub_type: 杂志文章,评审
doi:10.1016/j.neuroscience.2020.02.049
更新日期:2020-05-01 00:00:00
abstract::Presenilin-1 (PS1) is the gene responsible for the development of early-onset familial Alzheimer's disease. To probe the functions of PS1 on neuronal resistance to oxidative stress, we pharmacologically examined the death signals in PS1-deficient neurons induced by oxidative stress. Because the death of primarily cult...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2004.01.016
更新日期:2004-01-01 00:00:00
abstract::Previous research has implicated hippocampal rhythmical slow activity in the mechanisms of action of the anxiolytic drugs. In this study ethanol and a putative ethanol antagonist, Ro 15-4513, were investigated with reticular elicitation of rhythmical slow activity. Doses of ethanol between 0.6 and 3.1 g/kg were used. ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(90)90337-4
更新日期:1990-01-01 00:00:00
abstract::Turning in circles is among the behaviors elicited by unilateral cholinergic stimulation of the substantia nigra. Recent studies have shown that microinjection of cholinergic agonists into the substantia nigra pars compacta increases dopamine release and turnover in the striatum of anesthetized rats [Hernández-López e...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(94)90378-6
更新日期:1994-09-01 00:00:00
abstract::Astrocytes in the somatosensory ventrobasal (VB) thalamus of rats respond to glutamatergic synaptic input with metabotropic glutamate receptor (mGluR) mediated intracellular calcium ([Ca²⁺](i)) elevations. Astrocytes in the VB thalamus also release the gliotransmitter (GT) glutamate in a Ca²⁺-dependent manner. The tri...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2011.12.049
更新日期:2012-03-15 00:00:00
abstract::Deafferentation of the adult rat dentate gyrus induces reactive axonal growth by surviving afferent systems. In middle aged and aged rats, axonal sprouting is delayed and reduced relative to young adults. The cause for this age-related decline is not known, but it may reflect a decrement in trophic signals which initi...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(97)00539-3
更新日期:1998-04-01 00:00:00
abstract::The present study investigated the membrane mechanisms underlying the inhibitory influence of melatonin on suprachiasmatic nucleus (SCN) neurones in a hypothalamic slice preparation. Perforated-patch recordings were performed to prevent the rapid rundown of spontaneous firing rate as observed during whole cell recordi...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(01)00346-3
更新日期:2001-01-01 00:00:00
abstract::Within the dorsomedial hypothalamus (DMH), cholecystokinin (CCK) has been proposed to modulate neuropeptide Y (NPY) signaling to affect food intake. However, the neural circuitry underlying the actions of this CCK-NPY signaling system in the controls of food intake has yet to be determined. We sought to characterize t...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2007.12.004
更新日期:2008-03-03 00:00:00
abstract::This report presents the major structural features of neurons and their afferent input in nucleus magnocellularis, the avian homologue of the mammalian anteroventral cochlear nucleus. Results of light-microscope observations, as seen in Golgi, Nissl, and normal fiber preparations, as well as ultrastructural morphology...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(82)90045-8
更新日期:1982-04-01 00:00:00
abstract::The existence of a sex difference in several chronic pain syndromes and the fluctuation of symptoms during the menstrual cycle strongly suggest sex hormones are involved in pain processing. The mechanisms underlying these changes are not well understood. Using the colorectal distention model in the rat, we previously ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2008.04.070
更新日期:2008-07-17 00:00:00
abstract::Excitability and axon/dendrite specification are the most distinctive features in the establishment of neuronal polarization. Conditioned medium from rat sciatic nerve (CM) induced a neuronal-like morphology in PC12 cells. Here we show that CM neuritogenic activity is limited to the induction of dendrites in PC12 cell...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2008.12.063
更新日期:2009-03-17 00:00:00
abstract::A few hours after peripheral axons of cutaneous afferent neurons have been transected, some of their novel endings become excitable by physical or chemical stimuli. It has been assumed that these axon endings preferentially respond to those stimuli which have excited their previous receptive endings. We studied the pr...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(99)00325-5
更新日期:1999-01-01 00:00:00
abstract::Fat, ethanol, and nicotine share a number of properties, including their ability to reinforce behavior and produce overconsumption. To test whether these substances act similarly on the same neuronal populations in specific brain areas mediating these behaviors, we administered the substances short-term, using the sam...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2014.07.050
更新日期:2014-09-26 00:00:00
abstract::The present study utilized an in vitro preparation of the rabbit carotid body, with tissue catecholamine stores labeled by incubation with 3H-tyrosine. The goal was to characterize pharmacologically the voltage-dependent Ca2+ channels present in the type I (glomus) cells of this arterial chemoreceptor organ, and to el...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(92)90260-9
更新日期:1992-01-01 00:00:00
abstract::This study examined the localization and functional expression of ryanodine receptors (RyR) within the cochlea using a combination of reverse transcription-polymerase chain reaction, immunolabeling techniques, and confocal Ca2+ imaging. All three RyR isoform mRNA transcripts were detected in the adult rat cochlea. Imm...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2005.09.011
更新日期:2006-01-01 00:00:00
abstract::Certain patterns of neural activity can induce N-methyl-D-aspartic acid receptor (NMDAR)-dependent synaptic plasticity, one of the important foundations of memory. Here, we report that a patterned high-frequency stimulation (PHS) induces rat hippocampal long-term depression (LTD) in an NMDAR-independent manner that re...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2013.07.059
更新日期:2013-10-10 00:00:00
abstract::Free intracellular calcium concentration ([Ca2+]in) was recorded at 22 degrees C by means of Indo-1 or Fura-2 single-cell microfluorometry in cultured dorsal root ganglion neurons obtained from neonatal rats. The resting [Ca2+]in in dorsal root ganglion neurons was 73 +/- 21 nM (mean +/- S.D., n = 94). Fast applicatio...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(93)90029-f
更新日期:1993-12-01 00:00:00
abstract::The novel multifunctional brain permeable iron, chelator M30 [5-(N-methyl-N-propargyaminomethyl)-8-hydroxyquinoline] was shown to possess neuroprotective activities in vitro and in vivo, against several insults applicable to various neurodegenerative diseases, such as Alzheimer's disease, Parkinson's disease, and amyo...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2011.03.040
更新日期:2011-08-25 00:00:00
abstract::The development of chronic pain is associated with activity-dependent plastic changes in neuronal structures in the peripheral and central nervous system. In order to investigate the time-dependent processing of afferent noxious stimuli in the spinal cord we employed the quantitative autoradiographic 2-deoxyglucose te...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(99)00186-4
更新日期:1999-01-01 00:00:00
abstract::A number of viral vectors are currently being evaluated as potential gene therapy vectors for gene delivery to the brain. As well as evaluating their ability to express a transgene for extended periods of time it is also essential to examine any cytotoxic immune response to such vectors as this may not only limit tran...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(97)00513-7
更新日期:1998-04-01 00:00:00