Melatonin generates an outward potassium current in rat suprachiasmatic nucleus neurones in vitro independent of their circadian rhythm.


:The present study investigated the membrane mechanisms underlying the inhibitory influence of melatonin on suprachiasmatic nucleus (SCN) neurones in a hypothalamic slice preparation. Perforated-patch recordings were performed to prevent the rapid rundown of spontaneous firing rate as observed during whole cell recordings and to preserve circadian rhythmicity in SCN neurones. In current-clamp mode melatonin (1 microM or 1 nM) application, in the presence of agents that block action potential generation and fast synaptic transmission, resulted in a membrane hyperpolarisation accompanied with a decrease in input resistance in the majority of SCN neurones (71-86%). The amplitude of the hyperpolarisation was not found to be significantly different between circadian time 5-12 and 14-21. In voltage-clamp mode melatonin (1 microM or 1 nM) induced an outward current accompanied with an increase in membrane conductance. The current was found to be mainly potassium driven with voltage kinetics resembling those of an open rectifying potassium conductance. Investigations into the signal transduction mechanism revealed melatonin-induced inhibition of SCN neurones to be sensitive to pertussis toxin but independent of intracellular cAMP levels and phospholipase C activity. The present study shows that melatonin, at night-time physiological concentrations, reduces the neuronal excitability of the majority of SCN neurones independent of the time of application in the circadian cycle. Thus in vivo melatonin may be important for circadian time-keeping by amplifying the circadian rhythm in SCN neurones, by lowering their sensitivity to phase-shifting stimuli occurring at night.






van den Top M,Buijs RM,Ruijter JM,Delagrange P,Spanswick D,Hermes ML





Has Abstract


2001-01-01 00:00:00














  • Modulation of beta oscillations in the subthalamic area during action observation in Parkinson's disease.

    abstract::Mapping observed actions into the onlooker's motor system seems to provide the neurofunctional mechanisms for action understanding. Subthalamic nucleus (STN) local field potential (LFP) recordings in patients with movement disorders disclosed that network oscillations in the beta range are involved in conveying motor ...


    pub_type: 杂志文章


    authors: Marceglia S,Fiorio M,Foffani G,Mrakic-Sposta S,Tiriticco M,Locatelli M,Caputo E,Tinazzi M,Priori A

    更新日期:2009-07-21 00:00:00

  • Alterations in primary motor cortex neurotransmission and gene expression in hemi-parkinsonian rats with drug-induced dyskinesia.

    abstract::Treatment of Parkinson's disease (PD) with dopamine replacement relieves symptoms of poverty of movement, but often causes drug-induced dyskinesias. Accumulating clinical and pre-clinical evidence suggests that the primary motor cortex (M1) is involved in the pathophysiology of PD and that modulating cortical activity...


    pub_type: 杂志文章


    authors: Lindenbach D,Conti MM,Ostock CY,Dupre KB,Bishop C

    更新日期:2015-12-03 00:00:00

  • Secretoneurin--a neuropeptide generated in brain, adrenal medulla and other endocrine tissues by proteolytic processing of secretogranin II (chromogranin C).

    abstract::Secretogranin II (chromogranin C), originally described as tyrosine sulfated protein of the anterior pituitary, is present in large dense core vesicles of several endocrine cells and neurons. We raised antisera in rabbits to conjugates of two synthetic peptides (bovine secretogranin 133-151 and rat secretogranin 154-1...


    pub_type: 杂志文章


    authors: Kirchmair R,Hogue-Angeletti R,Gutierrez J,Fischer-Colbrie R,Winkler H

    更新日期:1993-03-01 00:00:00

  • D2 autoreceptor inhibition reveals oxygen-glucose deprivation-induced release of dopamine in guinea-pig cochlea.

    abstract::Dopamine (DA), released from the lateral olivocochlear (LOC) efferent terminals, the efferent arm of the short-loop feedback in the cochlea, is considered as a protective factor in the inner ear since it inhibits auditory nerve dendrite firing in ischemia- or noise-induced excitotoxicity leading to sensorineural heari...


    pub_type: 杂志文章


    authors: Halmos G,Doleviczényi Z,Répássy G,Kittel A,Vizi ES,Lendvai B,Zelles T

    更新日期:2005-01-01 00:00:00

  • Reduced number of neurons in the hippocampus and the cerebellum in the postnatal guinea-pig following intrauterine growth-restriction.

    abstract::Intrauterine growth restriction is a risk factor for neurological and behavioural deficits in children although the precise underlying biological correlate for this is unclear. The present study shows that animals with intrauterine growth restriction, induced by a period of reduced placental blood flow during the seco...


    pub_type: 杂志文章


    authors: Mallard C,Loeliger M,Copolov D,Rees S

    更新日期:2000-01-01 00:00:00

  • Neural correlates of the semantic interference effect: new evidence from transcranial direct current stimulation.

    abstract::In two experiments, we combined a semantic blocked naming paradigm with anodal transcranial direct current stimulation (tDCS) to shed light on the neural correlates of the semantic interference (SI) effect. In particular, prior to the naming task, anodal tDCS was applied over the left superior temporal gyrus (STG, Exp...


    pub_type: 杂志文章


    authors: Pisoni A,Papagno C,Cattaneo Z

    更新日期:2012-10-25 00:00:00

  • Continuous treatment with the D2 dopamine receptor agonist quinpirole decreases D2 dopamine receptors, D2 dopamine receptor messenger RNA and proenkephalin messenger RNA, and increases mu opioid receptors in mouse striatum.

    abstract::Dopamine-mediated behaviors and certain biochemical and molecular events associated with these behaviors were examined following continuous infusion of the D1 dopamine agonist SKF38393 or the D2 dopamine agonist quinpirole into mice for six days. SKF38393 produced a transient grooming behavior while quinpirole initial...


    pub_type: 杂志文章


    authors: Chen JF,Aloyo VJ,Weiss B

    更新日期:1993-06-01 00:00:00

  • Interleukin-1 beta induces long-term increase of axonally transported opiate receptors and substance P.

    abstract::Interleukin-1 is known to exert pleiotropic effects in host defence mechanisms and in inflammation. Chronic pain, inflammation and interleukin-1 beta enhance the production of substance P. Recently, axonal transport of opiate receptors was found to increase in rat sciatic nerves in the model of Freund's adjuvant-induc...


    pub_type: 杂志文章


    authors: Jeanjean AP,Moussaoui SM,Maloteaux JM,Laduron PM

    更新日期:1995-09-01 00:00:00

  • Behavioural and neurochemical consequences of chronic gut microbiota depletion during adulthood in the rat.

    abstract::Gut microbiota colonization is a key event for host physiology that occurs early in life. Disruption of this process leads to altered brain development which ultimately manifests as changes in brain function and behaviour in adulthood. Studies using germ-free (GF) mice highlight the extreme impact on brain health that...


    pub_type: 杂志文章


    authors: Hoban AE,Moloney RD,Golubeva AV,McVey Neufeld KA,O'Sullivan O,Patterson E,Stanton C,Dinan TG,Clarke G,Cryan JF

    更新日期:2016-12-17 00:00:00

  • Schizophrenia affects speech-induced functional connectivity of the superior temporal gyrus under cocktail-party listening conditions.

    abstract::The superior temporal gyrus (STG) is involved in speech recognition against informational masking under cocktail-party-listening conditions. Compared to healthy listeners, people with schizophrenia perform worse in speech recognition under informational speech-on-speech masking conditions. It is not clear whether the ...


    pub_type: 杂志文章


    authors: Li J,Wu C,Zheng Y,Li R,Li X,She S,Wu H,Peng H,Ning Y,Li L

    更新日期:2017-09-17 00:00:00

  • Comparative immunohistochemical localisation of GABA(B1a), GABA(B1b) and GABA(B2) subunits in rat brain, spinal cord and dorsal root ganglion.

    abstract::GABA(B) receptors are G-protein-coupled receptors mediating the slow onset and prolonged synaptic actions of GABA in the CNS. The recent cloning of two genes, GABA(B1) and GABA(B2), has revealed a novel requirement for GABA(B) receptor signalling. Studies have demonstrated that the two receptor subunits associate as a...


    pub_type: 杂志文章


    authors: Charles KJ,Evans ML,Robbins MJ,Calver AR,Leslie RA,Pangalos MN

    更新日期:2001-01-01 00:00:00

  • Nitric oxide-dependent long-term potentiation is blocked by a specific inhibitor of soluble guanylyl cyclase.

    abstract::The diffusible second messenger, nitric oxide, is synthesised in central neurons in response to activation of glutamate receptors or other stimuli that increase cytosolic Ca2+ concentrations. Among the many roles suggested for nitric oxide in the central nervous system is that of mediating synaptic plasticity. For exa...


    pub_type: 杂志文章


    authors: Boulton CL,Southam E,Garthwaite J

    更新日期:1995-12-01 00:00:00

  • Bradykinin-induced release of calcitonin gene-related peptide from capsaicin-sensitive nerves in guinea-pig atria: mechanism of action and calcium requirements.

    abstract::The mechanism of neuropeptide secretion induced by bradykinin from capsaicin-sensitive afferents was studied in guinea-pig atria. Both the inotropic response induced by bradykinin (0.1 microM) in the electrically driven isolated guinea-pig left atria and the bradykinin (10 microM)-induced release of calcitonin gene-re...


    pub_type: 杂志文章


    authors: Geppetti P,Tramontana M,Santicioli P,Del Bianco E,Giuliani S,Maggi CA

    更新日期:1990-01-01 00:00:00

  • The genetics of bipolar disorder.

    abstract::Bipolar disorder is a mood disorder characterized by impairing episodes of mania and depression. Twin studies have established that bipolar disorder is among the most heritable of medical disorders and efforts to identify specific susceptibility genes have intensified over the past two decades. The search for genes in...


    pub_type: 杂志文章,评审


    authors: Barnett JH,Smoller JW

    更新日期:2009-11-24 00:00:00

  • Topographical representation of vestibulo-ocular reflexes in rabbit cerebellar flocculus.

    abstract::In anaesthetized albino rabbits, the cerebellar flocculus was systematically mapped with a glass microelectrode to identify the location of Purkinje cells that inhibit specific vestibulo-ocular reflex pathways. The effects of microstimulation of the flocculus Purkinje cell layer on vestibular nerve-evoked reflexes to ...


    pub_type: 杂志文章


    authors: Ito M,Orlov I,Yamamoto M

    更新日期:1982-07-01 00:00:00

  • Contribution of astrocyte glycogen stores to progression of spreading depression and related events in hippocampal slices.

    abstract::Spreading depression (SD) is a wave of coordinated cellular depolarization that propagates slowly throughout brain tissue. SD has been associated with migraine aura, and related events have been implicated in the enlargement of some brain injuries. Selective disruption of astrocyte oxidative metabolism has previously ...


    pub_type: 杂志文章


    authors: Seidel JL,Shuttleworth CW

    更新日期:2011-09-29 00:00:00

  • Implication of Anterior Nucleus of the Thalamus in Mesial Temporal Lobe Seizures.

    abstract::Deep brain stimulation of the anterior nucleus of the thalamus has been proposed as novel therapy to treat intractable epilepsy. To optimize this approach, we proposed to study the involvement of this nucleus in a non-human primate model of mesial temporal lobe seizure. Two macaques were implanted with one chronic ele...


    pub_type: 杂志文章


    authors: Sherdil A,Coizet V,Pernet-Gallay K,David O,Chabardès S,Piallat B

    更新日期:2019-10-15 00:00:00

  • Gene expression profile analysis of epilepsy-associated gangliogliomas.

    abstract::Gangliogliomas (GG) constitute the most frequent tumor entity in young patients undergoing surgery for intractable epilepsy. The histological composition of GG, with the presence of dysplastic neurons, corroborates their maldevelopmental origin. However, their histogenesis, the pathogenetic relationship with other dev...


    pub_type: 杂志文章


    authors: Aronica E,Boer K,Becker A,Redeker S,Spliet WG,van Rijen PC,Wittink F,Breit T,Wadman WJ,Lopes da Silva FH,Troost D,Gorter JA

    更新日期:2008-01-02 00:00:00

  • Biochemical and anatomical characterization of forepaw adjusting steps in rat models of Parkinson's disease: studies on medial forebrain bundle and striatal lesions.

    abstract::Deficits in forepaw adjusting steps in rats have been proposed as a non-drug-induced model of the akinesia associated with Parkinson's disease. The present study examined the relationship between contralateral forepaw adjusting steps and dopamine depletion after medial forebrain bundle lesions with 6-hydroxydopamine. ...


    pub_type: 杂志文章


    authors: Chang JW,Wachtel SR,Young D,Kang UJ

    更新日期:1999-01-01 00:00:00

  • Investigating motor initiation and inhibition deficits in patients with Parkinson's disease and freezing of gait using a virtual reality paradigm.

    abstract::Freezing of gait (FOG) is a common, disabling symptom of Parkinson's disease (PD) that is associated with deficits in motor initiation and inhibition. Understanding of underlying neurobiological mechanisms has been limited by difficulties in eliciting and objectively characterizing such gait phenomena in the clinical ...


    pub_type: 杂志文章


    authors: Georgiades MJ,Gilat M,Ehgoetz Martens KA,Walton CC,Bissett PG,Shine JM,Lewis SJ

    更新日期:2016-11-19 00:00:00

  • Protein cysteine S-nitrosylation inhibits vesicular uptake of neurotransmitters.

    abstract::Previous studies have shown that nitric oxide can induce cysteine S-nitrosylation of total protein in synaptosomes, suggesting that nitric oxide may contribute to the regulation of synaptic protein function. Vesicular neurotransmitter transporters pack neurotransmitters into synaptic vesicles and play an important rol...


    pub_type: 杂志文章


    authors: Wang Y,Zhou Z,Leylek T,Tan H,Sun Y,Parkinson FE,Wang JF

    更新日期:2015-12-17 00:00:00

  • Changes in ubiquitin immunoreactivity in developing rat brain: a putative role for ubiquitin and ubiquitin conjugates in dendrite outgrowth and differentiation.

    abstract::The role of ubiquitin in development of the mammalian brain has been studied using a monoclonal antibody, RHUb1, specific for ubiquitin. Immunodevelopment of western blots of homogenate samples of the cerebral cortex, hippocampus and cerebellum prepared from animals of known postnatal age show marked developmental cha...


    pub_type: 杂志文章


    authors: Flann S,Hawkes RB,Riederer BM,Rider CC,Beesley PW

    更新日期:1997-11-01 00:00:00

  • Extracellular dopamine in striatum: influence of nerve impulse activity in medial forebrain bundle and local glutamatergic input.

    abstract::Microdialysis probes were used to measure dopamine in, and to administer glutamate receptor antagonists and agonists to, the striatum of unanesthetized rats. Antagonists used were: kynurenate, 2-amino-5-phosphonovalerate and 6-cyano-7-nitroquinoxaline-2,3-dione. Agonists used were: N-methyl-D-aspartate and kainate. In...


    pub_type: 杂志文章


    authors: Keefe KA,Zigmond MJ,Abercrombie ED

    更新日期:1992-01-01 00:00:00

  • Epileptic focus induced in rat by intrahippocampal cholera toxin: neuronal properties in vitro.

    abstract::Injecting 0.5-1.0 microgram of cholera toxin into rat hippocampus induces a chronic epileptic focus which generates interictal discharges and brief epileptic seizures intermittently over the following seven to 10 days. Here we examined the electrophysiological properties of hippocampal slices prepared from these rats ...


    pub_type: 杂志文章


    authors: Watts AE,Whittington MA,Jefferys JG

    更新日期:1993-07-01 00:00:00

  • Differential expression of synapsin genes during early zebrafish development.

    abstract::Synapsins are a family of synaptic vesicle (SV)-associated phosphoproteins that have been identified in several vertebrates and invertebrates. We report here the cloning and expression of synapsin family genes in the zebrafish Danio rerio. We identified the complete coding sequence of synapsin 3, which is not present ...


    pub_type: 杂志文章


    authors: Garbarino G,Costa S,Pestarino M,Candiani S

    更新日期:2014-11-07 00:00:00

  • A key role for the caudoventral pontine tegmentum in the simultaneous generation of eye saccades in bursts and associated ponto-geniculo-occipital waves during paradoxical sleep in the cat.

    abstract::Ponto-geniculo-occipital waves and rapid eye movements (eye saccades) are two prominent phasic events of paradoxical sleep which occur in conjunction. Although they have been studied intensively, the neuronal link between these two events is still poorly understood. On the basis of our previous results, combining brai...


    pub_type: 杂志文章


    authors: Vanni-Mercier G,Debilly G

    更新日期:1998-09-01 00:00:00

  • Analysis of excitatory synapses in the guinea pig inferior colliculus: a study using electron microscopy and GABA immunocytochemistry.

    abstract::The inferior colliculus (IC) integrates ascending auditory input from the lower brainstem and descending input from the auditory cortex. Understanding how IC cells integrate these inputs requires identification of their synaptic arrangements. We describe excitatory synapses in the dorsal cortex, central nucleus, and l...


    pub_type: 杂志文章


    authors: Nakamoto KT,Mellott JG,Killius J,Storey-Workley ME,Sowick CS,Schofield BR

    更新日期:2013-05-01 00:00:00

  • Depolarization of rat locus coeruleus neurons by adenosine 5'-triphosphate.

    abstract::Intracellular recordings were performed in a pontine slice preparation of the rat brain containing the locus coeruleus. The enzymatically stable P2-purinoceptor agonist alpha,beta-methylene ATP increased the firing rate without altering the amplitude or shape of action potentials; the afterhyperpolarization following ...


    pub_type: 杂志文章


    authors: Harms L,Finta EP,Tschöpl M,Illes P

    更新日期:1992-06-01 00:00:00

  • Architectonic mapping of the medial region of the human orbitofrontal cortex by density profiles.

    abstract::The study of architectonic differentiation in the cortex is advanced by the articulation of objective definitions based on clear features of the cortical architecture. We adopted areal density profiles as a means of sampling the cortex. On the profiles, we isolated and quantified the density of individual cortical lay...


    pub_type: 杂志文章


    authors: Mackey S,Petrides M

    更新日期:2009-03-31 00:00:00

  • Swimming Physical Training Prevented the Onset of Acute Muscle Pain by a Mechanism Dependent of PPARγ Receptors and CINC-1.

    abstract::Regular physical exercise has been described as a good strategy for prevention or reduction of musculoskeletal pain. The Peroxisome Proliferator-Activated Receptor Gamma (PPARγ) has been investigated as a promising target for the control of inflammatory pain. Therefore, the aim of this study was to evaluate whether ac...


    pub_type: 杂志文章


    authors: de Azambuja G,Botasso Gomes B,Messias LHD,Aquino BM,Jorge CO,Manchado-Gobatto FB,Oliveira-Fusaro MCG

    更新日期:2020-02-10 00:00:00