Abstract：
:A variety of neurological diseases are characterized by disturbances of the blood-brain barrier (BBB) and its transporters. We recently introduced fibroblast growth factor treated cortical organotypic slice cultures of mice as a model for in vitro studies of the blood-brain barrier and have now further characterized the maintenance and function of transport-proteins typically expressed in the endothelium of cerebral blood vessels. The glucose transporter GLUT-1 is present in blood vessels of slice cultures derived from postnatal day 4 to 21 mice after 3 days in vitro. The endothelial multidrug resistance P-glycoprotein (P-gp) which is involved in the control of pharmacological substance transport across the blood-brain barrier is also maintained in blood vessels, most prominently in slice cultures derived from postnatal day 14 and 21 mice. To assess P-gp function, we tested rhodamine 123 transport in presence or absence of the P-gp inhibitor verapamil. Rhodamine 123-fluorescence accumulated rapidly in the vascular lumen both in acute slices and in slices cultured for 3 days in vitro. Our results provide evidence that endothelial transporters and their functional properties can be maintained in organotypic cortical slices cultures, thus making it an attractive model system for the study of the blood-brain barrier.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Camenzind RS,Chip S,Gutmann H,Kapfhammer JP,Nitsch C,Bendfeldt Kdoi
10.1016/j.neuroscience.2010.06.073subject
Has Abstractpub_date
2010-09-29 00:00:00pages
361-71issue
1eissn
0306-4522issn
1873-7544pii
S0306-4522(10)00945-0journal_volume
170pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract：:The superior colliculus sends a climbing fiber output to cerebellar vermal lobules VI-VII through the inferior olive. The present study in cats morphologically clarified the existence of a topographical organization in the tecto-olivo-cerebellar projection. A horseradish peroxidase study on the tecto-olivary projectio...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(91)90212-7
更新日期：1991-01-01 00:00:00
abstract：:The present studies were conducted to test the hypothesis that systemically inactive doses of cannabinoids suppress inflammation-evoked neuronal activity in vivo via a peripheral mechanism. We examined peripheral cannabinoid modulation of spinal Fos protein expression, a marker of neuronal activity, in a rat model of ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(02)00870-9
更新日期：2003-01-01 00:00:00
abstract：:Microdialysis probes were used to measure dopamine in, and to administer glutamate receptor antagonists and agonists to, the striatum of unanesthetized rats. Antagonists used were: kynurenate, 2-amino-5-phosphonovalerate and 6-cyano-7-nitroquinoxaline-2,3-dione. Agonists used were: N-methyl-D-aspartate and kainate. In...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(92)90248-z
更新日期：1992-01-01 00:00:00
abstract：:The glycine transporter GLYT2 is present in axonal boutons throughout the spinal cord, and its laminar distribution matches that of glycine-enriched axons, which are presumed to be glycinergic. In order to determine whether boutons which possess GLYT2 are glycine-enriched, we have carried out pre-embedding immunocytoc...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(96)00501-5
更新日期：1997-03-01 00:00:00
abstract：:The human brain is composed of complex networks of 100 billion neurons that underlie its higher functions. The set of neural connections in the brain has recently attracted growing interest from the scientific community. It is important to identify individual differences in these neural connections to study the backgr...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2015.06.032
更新日期：2015-08-20 00:00:00
abstract：:Cognitive impairment is one of the common non-motor symptoms in Parkinson's disease (PD). The hippocampus is a critical structure for learning and memory processes. The abnormal synaptic plasticity in the hippocampus is suggested to be associated with cognitive dysfunction in PD. Voltage gated sodium channels (VGSCs) ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2019.08.024
更新日期：2019-10-15 00:00:00
abstract：:Lamina I of the spinal dorsal horn contains a diverse mixture of neurons. Among these, a group of giant neurons (Waldeyer cells) has long been recognized. In this study we have used immunocytochemistry to characterize a population of Waldeyer cells which were identified by the presence of high levels of the glycine re...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(00)00445-0
更新日期：2001-01-01 00:00:00
abstract：:Brain-derived neurotrophic factor (BDNF) is a neurotrophin (NT) known to participate in chronic somatic pain. A recent study has indicated that BDNF may participate in chronic cystitis at the peripheral level. However, the principal site of action for this NT is the central nervous system, most notably the spinal cord...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2012.12.044
更新日期：2013-03-27 00:00:00
abstract：:In the present study, we found that amyloid-beta peptide enhanced glutamate release from primary cultured rat microglia via the Na+-dependent glutamate transporter, which was activated by extracellular K+. Glutamate transport current was measured by a conventional whole-cell patch recording mode under voltage-clamp co...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(99)00036-6
更新日期：1999-01-01 00:00:00
abstract：:Dopaminergic compounds often affect the unlearned behaviors of preweanling and adult rats differently, although the brain regions underlying these age-dependent behavioral effects have not been specified. A candidate brain region is the dorsal caudate-putamen (CPu); thus, a goal of the present study was to determine w...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2011.03.037
更新日期：2011-06-02 00:00:00
abstract：:Repeated induction of a temporary threshold shift (TTS) may result in a permanent threshold shift (PTS) and is thought to be associated with early onset of age-related hearing loss (ARHL). The possibility that a PTS might be induced by administration of repeated TTS-inducing noise exposures (NEs) over a short period d...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2019.12.049
更新日期：2020-03-01 00:00:00
abstract：:Phenomics is an emerging transdiscipline dedicated to the systematic study of phenotypes on a genome-wide scale. New methods for high-throughput genotyping have changed the priority for biomedical research to phenotyping, but the human phenome is vast and its dimensionality remains unknown. Phenomics research strategi...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2009.01.027
更新日期：2009-11-24 00:00:00
abstract：:An increased frequency of apolipoprotein E E4 allele has been reported in patients with late onset Alzheimer's disease. Apolipoprotein E participates in the transport of cholesterol and other lipids and interferes with the growth and regeneration of both peripheral and central nervous system tissues during development...
journal_title：Neuroscience
pub_type： 临床试验,杂志文章
doi：10.1016/0306-4522(95)00014-a
更新日期：1995-07-01 00:00:00
abstract：:GABAergic neurons occur in the myenteric plexus and submucosa and their innervations of the gut, where GABA stimulates motor neurons, and non-neural cells via "central type" GABA(A) receptors. These receptors occur on half of the neurons in the rat intestine. The GABA(A) receptor is a ligand-gated chloride channel con...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(99)00174-8
更新日期：1999-01-01 00:00:00
abstract：:Traumatic brain injury (TBI) is a major cause of morbidity and mortality world-wide and can result in persistent cognitive, sensory and behavioral dysfunction. Understanding the time course of TBI-induced pathology is essential to effective treatment outcomes. We induced TBI in rats using an impact acceleration method...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2016.08.018
更新日期：2016-10-15 00:00:00
abstract：:The paraventricular nucleus of the hypothalamus is increasingly being viewed as an important site for cardiovascular integration because of its connections to regions in the brain and spinal cord which are known to be important in cardiovascular control. Like the vasomotor neurons of the rostral ventrolateral medulla,...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(98)00255-3
更新日期：1999-01-01 00:00:00
abstract：:Neurons depend upon the processes of axonal and transneuronal transport for intra- and intercellular communication and trophic support. Experimental studies in the last decade have elucidated the mechanisms underlying these processes, and provided evidence for their role in the spread of viral and toxic diseases throu...
journal_title：Neuroscience
pub_type： 杂志文章,评审
doi：10.1016/0306-4522(87)90063-7
更新日期：1987-11-01 00:00:00
abstract：:Humans perform object recognition effortlessly and accurately. However, it is unknown how the visual system copes with variations in objects' appearance and the environmental conditions. Previous studies have suggested that affine variations such as size and position are compensated for in the feed-forward sweep of vi...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2017.02.050
更新日期：2017-05-04 00:00:00
abstract：:Neural injury triggers changes in the expression of a large number of gene families. Particularly interesting are those encoding proteins involved in the generation, propagation or restoration of electric potentials. The expression of the Na+, K+-ATPase subunit isoforms (alpha, beta and gamma) was studied in dorsal ro...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2004.08.041
更新日期：2004-01-01 00:00:00
abstract：:Synapsins (SynI, SynII, SynIII) are a multigene family of synaptic vesicle (SV) phosphoproteins implicated in the regulation of synaptic transmission and plasticity. Synapsin I, II, I/II and I/II/III knockout mice are epileptic and SYN1/2 genes have been identified as major epilepsy susceptibility genes in humans. We ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2010.08.046
更新日期：2010-11-24 00:00:00
abstract：:Sestrin2 (Sesn2) is a stress response protein which expresses neuroprotective characteristics in some neurodegenerative disorders. However, the impact of Sesn2 on the clinical outcome of stroke is unclear. The nuclear factor-erythroid 2 related factor 2/heme oxygenase-1 (Nrf2/HO-1) pathway is a key factor in angiogene...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2019.05.005
更新日期：2019-07-01 00:00:00
abstract：:Modulation of soluble guanylate cyclase (sGC) by nitric oxide (NO) is altered in brain from cirrhotic patients. The aim of this work was to assess whether an animal model of cirrhosis, bile duct ligation, alone or combined with diet-induced hyperammonemia for 7-10 days reproduces the alterations in NO modulation of sG...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2004.09.027
更新日期：2005-01-01 00:00:00
abstract：:Stimulation of cervical vagal afferents inhibits central sympathetic outflows in part by inhibiting the ongoing activity of putative baroreceptive neurons in the rostral ventrolateral medulla oblongata. The aim of the present study was to examine the electrophysiological characteristics of vagal responses and their in...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(99)00041-x
更新日期：1999-01-01 00:00:00
abstract：:The neurotransmitter serotonin (5-HT) plays a central role in brain development, regulation of mood, stress reactivity and risk of psychiatric disorders, and thus alterations in 5-HT signaling early in life have critical implications for behavior and mental health across the life span. Drawing on preclinical and emerg...
journal_title：Neuroscience
pub_type： 杂志文章,评审
doi：10.1016/j.neuroscience.2016.02.037
更新日期：2017-02-07 00:00:00
abstract：:Our series of rat experiments have shown that locomotor activity, arousal level, body and brown adipose tissue temperatures, heart rate and arterial pressure increase episodically in an integrated manner approximately every 100min (ultradian manner). Although it has been proposed that the integrated ultradian pattern ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2016.07.041
更新日期：2016-10-15 00:00:00
abstract：:Olvanil ((N-vanillyl)-9-oleamide), a non-pungent transient receptor potential vanilloid type 1 agonist, desensitizes nociceptors and alleviates pain. But its molecular targets and signaling mechanisms are little known. Calcium influx through voltage-activated Ca(2+) channels plays an important role in neurotransmitter...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2006.03.023
更新日期：2006-08-11 00:00:00
abstract：:Antisera raised against protein-glutaraldehyde-amino acid conjugates were used to study the light and electron microscopic distribution of GABA, glutamate, glutamine and taurine in organotypic slice cultures of rat hippocampi. In the stratum oriens and radiatum, glutamate-like immunoreactivity was particularly concent...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(92)90187-7
更新日期：1992-01-01 00:00:00
abstract：:In the glomerular layer of the rat main olfactory bulb, we previously reported three chemically defined interneuron groups: GABA-like immunoreactive, calretinin-immunoreactive and Calbindin-D28k-immunoreactive groups [Kosaka K. et al. (1995) Neurosci. Res. 23, 73-88]. In the present study, we analysed the structural f...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(96)00308-9
更新日期：1997-02-01 00:00:00
abstract：:Major illnesses, including heart attack and sepsis, can cause cognitive impairments, depression, and progressive memory decline that persist long after recovery from the original illness. In rodent models of sepsis or subchronic immune challenge, memory deficits also persist for weeks or months, even in the absence of...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2019.12.019
更新日期：2020-01-21 00:00:00
abstract：:How seizures arise and recur in epilepsy is unknown. Recent genetic, pharmacological and electrophysiological data indicate a significant but undisclosed role for voltage-dependent calcium channels. Since the contribution such channels make to nerve function reflects the targeting of discrete subtypes to distinct cell...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(99)00371-1
更新日期：1999-01-01 00:00:00
