Abstract:
:A variability in DNA content detected was found with image cytometry, in immature bone marrow cells from 13 healthy donors (median age 31 yr). The mean coefficient of variation (C.V.) of the DNA content was found to be significantly (P = 0.0002) higher in immature blasts and promyelocytes than in mature granulocytes, lymphocytes, and monocytes. The finding was not due to high DNA contents secondary to DNA-synthesis in immature cells, since the percentage of such cells with a reduced DNA content was also significantly (P = 0.0424-0.0002) increased. The error of the method expressed as the variance of multiple measurements has been found to be 0.0002-0.0004. A staining error has been found for some hydrolysis times, but not for times between 60 and 120 minutes. A measuring error was found for an average of 1.38 to 3.38% of the cells. If many normal cells with DNA-aneuploidy are sterile, this would explain the present findings as well as previous ones about intra-marrow cell death, and also the fact that the variability in DNA content could not be detected with cytogenetic or flow cytometric methods.
journal_name
Am J Hematoljournal_title
American journal of hematologyauthors
Widell S,Auer G,Hast R,Reizenstein Pdoi
10.1002/ajh.2830430412subject
Has Abstractpub_date
1993-08-01 00:00:00pages
291-4issue
4eissn
0361-8609issn
1096-8652journal_volume
43pub_type
杂志文章abstract::Platelet antibodies are detectable in only about 50% of patients with chronic autoimmune thrombocytopenia (AITP). We determined platelet antibodies against GPIa/IIa, GPIb/IX, GPIIb/IIIa, and GPV and reticulated platelets in three female patients with AITP, before and after immunoadsorption treatment. None of the three...
journal_title:American journal of hematology
pub_type: 杂志文章
doi:10.1002/ajh.10312
更新日期:2003-05-01 00:00:00
abstract::While surgery is commonly required for complications related to hemoglobin SC (HbSC) disease, little is known about the perioperative complications or the indications for preoperative transfusion in this group. We describe the patient characteristics, preoperative transfusion regimens, and outcome in 92 patients with ...
journal_title:American journal of hematology
pub_type: 杂志文章
doi:10.1002/(sici)1096-8652(199802)57:2<101::aid-ajh2>
更新日期:1998-02-01 00:00:00
abstract::Cultured endothelial cells from bovine aorta were exposed to oxidized low density lipoprotein and examined by electron microscopy. The endothelial cells contracted slightly and the intercellular junctions became unclear. Some osmiophilic material increased in the cytoplasm. The oxidized low density lipoprotein appears...
journal_title:American journal of hematology
pub_type: 杂志文章
doi:10.1002/ajh.2830490315
更新日期:1995-07-01 00:00:00
abstract::Eosinophilia associated with solid tumors is an infrequent occurrence. The pathogenesis of tumor-associated eosinophilia is not well understood. Interleukin-5 (IL-5) is a cytokine that has been implicated in the development of eosinophilia in mice and humans. However, there is little data associating IL-5 production w...
journal_title:American journal of hematology
pub_type: 杂志文章
doi:10.1002/ajh.20789
更新日期:2007-03-01 00:00:00
abstract::Positron emission tomography-computed tomography (PET-CT) can identify bony lesions, assess disease burden, and detect extramedullary disease (EMD) in patients with multiple myeloma. We retrospectively reviewed records of patients who underwent PET-CT within 60 days of a new diagnosis (before therapy commenced) to ide...
journal_title:American journal of hematology
pub_type: 杂志文章
doi:10.1002/ajh.25279
更新日期:2018-12-01 00:00:00
abstract::Trimethoprim and sulfamethoxazole (Bactrim r) is a widely used antibiotic combination effective against a broad spectrum of microbial organisms. There are reports of neutropenia developing during even brief periods of oral therapy, particularly in individuals with either folate deficiency or increased folate requireme...
journal_title:American journal of hematology
pub_type: 杂志文章
doi:10.1002/ajh.2830230102
更新日期:1986-09-01 00:00:00
abstract::Thromboembolic complications following splenectomy for hematologic diseases occur in up to 10% of patients and may range from portal vein thrombosis (PVT) to pulmonary embolism (PE) and deep vein thrombosis (DVT). Up to now there exist no recommendations for the duration and intensity of prophylactic anticoagulation, ...
journal_title:American journal of hematology
pub_type: 杂志文章
doi:10.1002/ajh.20018
更新日期:2004-06-01 00:00:00
abstract::Increasing dose intensity (DI) of chemotherapy for patients with aggressive non-Hodgkin lymphoma (NHL) may improve outcomes at the cost of increased toxicity. This issue was addressed in a randomized trial aiming to double the DI of myelosuppressive drugs. Between 1994 and 1999, 250 patients with previously untreated ...
journal_title:American journal of hematology
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1002/ajh.23684
更新日期:2014-05-01 00:00:00
abstract::Hemophagocytic syndrome (HPS) is an unusual acute syndrome presenting with fever, hepatosplenomegaly, and cytopenias. The hallmark of HPS is the accumulation of activated macrophages that engulf hematopoietic cells in the reticuloendothelial system. Most cases of HPS in adults are secondary to infection or malignancy,...
journal_title:American journal of hematology
pub_type: 杂志文章
doi:10.1002/ajh.20123
更新日期:2004-08-01 00:00:00
abstract::The Sickle Cell Disease Clinical Research Network (SCDCRN) designed the PROACTIVE Feasibility Study (ClinicalTrials.gov NCT00951808) to determine whether elevated serum levels of secretory phospholipase A2 (sPLA2) during hospitalization for pain would permit preemptive therapy of sickle cell acute chest syndrome (ACS)...
journal_title:American journal of hematology
pub_type: 杂志文章
doi:10.1002/ajh.22265
更新日期:2012-03-01 00:00:00
abstract::We describe a 25-year-old black woman who presented with a long history of anemia requiring transfusions during childhood and adolescence. Molecular analysis revealed her to be a compound heterozygote for the sickle mutation and the approximately 22.7 kb deletion associated with hemoglobin Kenya. This patient's clinic...
journal_title:American journal of hematology
pub_type: 杂志文章
doi:10.1002/ajh.2830410413
更新日期:1992-12-01 00:00:00
abstract::We present a case of acquired von Willebrand syndrome (AVWS) due to a monoclonal gammopathy of undetermined significance. Initially this case was diagnosed as congenital von Willebrand disease (VWD); however, re-examination of the medical history rendered a congenital bleeding disorder unlikely. A normal plasma von Wi...
journal_title:American journal of hematology
pub_type: 杂志文章
doi:10.1002/ajh.20760
更新日期:2007-01-01 00:00:00
abstract::Because of the increase in the use of warfarin in the population in recent years, reversal of warfarin-related coagulopathy has become common in daily hospital practice. Transfusion of fresh frozen plasma (FFP) is the preferred treatment method for urgent warfarin reversal in the USA. We have undertaken a 1-month audi...
journal_title:American journal of hematology
pub_type: 杂志文章
doi:10.1002/ajh.20902
更新日期:2007-12-01 00:00:00
abstract::Hereditary spherocytosis (HS) is due to different membrane protein defects (i.e., deficiency of spectrin and ankyrin, band 3, or band 4.2). In order to gain new insight into the relationships between band 3 function and proteins associated with the cytoskeleton, we studied erythrocyte anion transport activity in HS ch...
journal_title:American journal of hematology
pub_type: 杂志文章
doi:10.1002/(sici)1096-8652(199707)55:3<121::aid-ajh1>
更新日期:1997-07-01 00:00:00
abstract:DISEASE OVERVIEW:Hodgkin lymphoma (HL) is an uncommon B-cell lymphoid malignancy affecting 8500 new patients annually and representing approximately 10.2% of all lymphomas in the United States. DIAGNOSIS:HL is composed of two distinct disease entities: classical HL and nodular lymphocyte predominant HL. Nodular sclero...
journal_title:American journal of hematology
pub_type: 杂志文章,评审
doi:10.1002/ajh.25071
更新日期:2018-05-01 00:00:00
abstract::Recent advances in massively parallel sequencing technologies have provided a detailed picture of the mutational landscape in CLL and underscored the vast degree of interpatient and intratumor heterogeneities. These studies have led to the characterization of novel putative driver genes and recurrently affected biolog...
journal_title:American journal of hematology
pub_type: 杂志文章,评审
doi:10.1002/ajh.24301
更新日期:2016-05-01 00:00:00
abstract::Ticlopidine-induced aplastic anemia (TIAA) is considered very uncommon. We present two new cases, and we review 55 additional cases from the literature. The first case concerns a 70-year-old man who developed severe aplastic anemia 7 weeks after treatment with 500 mg of ticlopidine daily. The patient sustained a sever...
journal_title:American journal of hematology
pub_type: 杂志文章,meta分析,评审
doi:10.1002/ajh.10150
更新日期:2002-09-01 00:00:00
abstract::Patients initially diagnosed with type 1 von Willebrand disease (VWD) have been reclassified as type 2 after a more exhaustive analysis in several studies. Our study's objectives were (1) to reanalyze patients that were previously diagnosed as type 1 to achieve a more accurate diagnosis and (2) to compare the von Will...
journal_title:American journal of hematology
pub_type: 杂志文章
doi:10.1002/ajh.20470
更新日期:2005-11-01 00:00:00
abstract::Specific anti-human thymus xenoantiserum (ATS) was utilized for characterizing a human thymus antigen (HTA) preferentially expressed on human thymocytes. Binding of ATS with different cell types was studied by immunofluorescence and immunoperoxidase techniques, as well as by radioimmunoprecipitation (RIP) followed by ...
journal_title:American journal of hematology
pub_type: 杂志文章
doi:10.1002/ajh.2830100206
更新日期:1981-01-01 00:00:00
abstract::Mutations at positions beta IVS1-6, beta IVS1-110, and beta 39 of the beta globin gene are responsible for the three most common thalassemic genes in the Mediterranean population. The polymerase chain reaction (PCR) was employed to amplify a 536 base pair segment surrounding this region. Nonradioactive labelling of an...
journal_title:American journal of hematology
pub_type: 杂志文章
doi:10.1002/ajh.2830340213
更新日期:1990-06-01 00:00:00
abstract::In some cases, bone marrow aplasia has been thought to result from immunologic abnormalities. Our patient had severe transfusion-dependent aplastic anemia, which responded to treatment with prednisone on two occasions. The exacerbations of aplastic anemia were associated with lymphocytic proliferation which on one occ...
journal_title:American journal of hematology
pub_type: 杂志文章
doi:10.1002/ajh.2830110310
更新日期:1981-11-01 00:00:00
abstract::A single-tube osmotic fragility test has been proposed for thalassemia screening with a range of different concentrations of saline having been employed. We have compared the sensitivity and specificity of 0.32%, 0.34%, and 0.36% buffered saline, and on the basis of our findings, recommend the use of 0.36% saline. Thi...
journal_title:American journal of hematology
pub_type: 杂志文章
doi:10.1002/ajh.20387
更新日期:2005-07-01 00:00:00
abstract::The ability of circulating progenitor cells to develop erythroid colonies was studied in vitro in the presence or absence of growth factors (5637-CM and erythropoietin) in 63 patients with sickle cell disease (SCD) (36 homozygotes for hemoglobin [Hb] S, 13 double heterozygotes for Hb S and beta thalassemia, and 14 SC ...
journal_title:American journal of hematology
pub_type: 杂志文章
doi:10.1002/(sici)1096-8652(199905)61:1<40::aid-ajh8>3
更新日期:1999-05-01 00:00:00
abstract::Intensive induction chemotherapy using anthracycline and cytarabine backbone is considered the most effective upfront therapy in physically fit older patients with acute myeloid leukemia (AML). However, outcomes of the standard induction in elderly AML are inferior to those observed in younger patients, and they are s...
journal_title:American journal of hematology
pub_type: 杂志文章,随机对照试验
doi:10.1002/ajh.24654
更新日期:2017-04-01 00:00:00
abstract::Iron deposition in combination with inflammatory and immunogenetic factors is involved in the pathophysiology of cardiac dysfunction in β-thalassemia major. We investigated the mechanical and endocrine function of the left atrium and ventricle to identify early signs of dysfunction. We studied 90 patients (mean age: 2...
journal_title:American journal of hematology
pub_type: 杂志文章
doi:10.1002/ajh.23581
更新日期:2014-01-01 00:00:00
abstract::A 1750 base pair cDNA to human terminal deoxynucleotidyl transferase (TdT) has been cloned. This cDNA detects a dominant 2200 base pair messenger RNA species in normal and leukemic cells synthesizing the enzyme. A quantitative dot blot assay was utilized to survey a number of clinical samples from patients with TdT po...
journal_title:American journal of hematology
pub_type: 杂志文章
doi:10.1002/ajh.2830250305
更新日期:1987-07-01 00:00:00
abstract::Reversible posterior leukoencephalopathy syndrome (RPLS) is an uncommon but distinctive clinicoradiological entity comprising of headache, seizures, visual disturbance, and altered mental function, in association with posterior cerebral white matter edema. With appropriate management, RPLS is reversible in the majorit...
journal_title:American journal of hematology
pub_type: 杂志文章
doi:10.1002/ajh.20147
更新日期:2004-09-01 00:00:00
abstract::In this study, we analyzed a thiotepa-based conditioning regimen for allogeneic stem cell transplantation in adults with acute lymphoblastic leukemia, using the EBMT database. A total of 323 patients were identified. The median age was 43 years. Disease status at transplant was first complete remission (CR1) in 48.9%,...
journal_title:American journal of hematology
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1002/ajh.24567
更新日期:2017-01-01 00:00:00
abstract::Evidence suggest that even patients aged 70 or above benefit from specific AML therapy. The fundamental decision in AML then becomes whether to recommend standard or investigational treatment. This decision must rest on the likely outcome of standard treatment. Hence we review factors that predict treatment related mo...
journal_title:American journal of hematology
pub_type: 杂志文章,评审
doi:10.1002/ajh.24439
更新日期:2016-08-01 00:00:00
abstract::Reduced intensity conditioning (RIC) regimens are widely used in allogeneic stem cell transplantation (SCT). In this study, we retrospectively investigated the clinical outcomes of RIC with fludarabine (Flu; 180 mg/m(2)), intravenous busulfan (BU; 6.4 mg/kg) or oral BU (8 mg/kg), and low-dose total body irradiation (T...
journal_title:American journal of hematology
pub_type: 杂志文章
doi:10.1002/ajh.21630
更新日期:2010-04-01 00:00:00