Abstract:
:The ability of circulating progenitor cells to develop erythroid colonies was studied in vitro in the presence or absence of growth factors (5637-CM and erythropoietin) in 63 patients with sickle cell disease (SCD) (36 homozygotes for hemoglobin [Hb] S, 13 double heterozygotes for Hb S and beta thalassemia, and 14 SC patients) in Southeast Brazil. In the presence of growth factors, SCD patients (all genotypes) presented significantly higher numbers of circulating burst-forming unit-erythroid (BFU-E/5 x 10(5) MNC), when compared with control subjects. However, when the progenitor cells were cultured in the absence of added stimulus, high numbers of BFU-E were observed only in the genotypes SS and S/beta thalassemia. SC patients presented a similar response to the control subjects. Moreover, there was an inverse correlation between spontaneous (without stimulus) BFU-E and Hb levels in SCD patients. These results suggest that the formation of spontaneous BFU-E observed in SCD may be due to an expanded erythropoiesis secondary to hemolysis.
journal_name
Am J Hematoljournal_title
American journal of hematologyauthors
Perlingeiro RC,Costa FF,Saad ST,Arruda VR,Queiroz MLdoi
10.1002/(sici)1096-8652(199905)61:1<40::aid-ajh8>3keywords:
subject
Has Abstractpub_date
1999-05-01 00:00:00pages
40-5issue
1eissn
0361-8609issn
1096-8652pii
10.1002/(SICI)1096-8652(199905)61:1<40::AID-AJH8>3journal_volume
61pub_type
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