Abstract:
:A single-tube osmotic fragility test has been proposed for thalassemia screening with a range of different concentrations of saline having been employed. We have compared the sensitivity and specificity of 0.32%, 0.34%, and 0.36% buffered saline, and on the basis of our findings, recommend the use of 0.36% saline. This gave definitely positive or equivocal results in 81 of 85 patients with beta thalassemia trait and in 4 of 4 with alpha(0) thalassemia trait. There were 14% false positive results in hematologically normal patients and 81% of the samples from patients with various variant hemoglobins gave positive results. The sensitivity was 95% and specificity 86%. The single-tube osmotic fragility test is potentially useful in under-resourced laboratories although it cannot replace automated red cell indices using electronic counters.
journal_name
Am J Hematoljournal_title
American journal of hematologyauthors
Chow J,Phelan L,Bain BJdoi
10.1002/ajh.20387keywords:
subject
Has Abstractpub_date
2005-07-01 00:00:00pages
198-201issue
3eissn
0361-8609issn
1096-8652journal_volume
79pub_type
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