Glycoconjugates in the cochlea as revealed by the silver methenamine method.

Abstract:

:The glycoconjugates in the cochlea of the guinea pig were studied by staining samples by the silver methenamine method as well as after periodic acid-Schiff (PAS) staining. Results obtained by the two methods were similar but not identical. The silver methenamine method was much better in terms of resolution. However, this method of staining seemed less specific than the PAS reaction. When the silver methenamine method was used, the tectorial membrane and outer hair cells were specifically stained. Two types of fibrils were observed in the tectorial membrane. Thick fibrils were located in the fibrous layer. Thin fibrils were situated in the marginal band, the cover net, Hensen's stripe and the fibrous layer. The thick and thin fibrils appeared to correspond to type A and type B protofibrils, respectively. The outer hair cells were found to contain strongly stained particles which, presumably, consisted of glycogen. The basement membrane of the capillaries in the stria vascularis also gave a positive reaction, while that of other capillaries was essentially unstained. This finding suggests structural differences between these capillaries.

journal_name

Hear Res

journal_title

Hearing research

authors

Kawamata S,Igarashi Y

doi

10.1016/0378-5955(93)90234-r

subject

Has Abstract

pub_date

1993-05-01 00:00:00

pages

75-82

issue

1-2

eissn

0378-5955

issn

1878-5891

pii

0378-5955(93)90234-R

journal_volume

67

pub_type

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