Abstract:
:We constructed an SV40-derived expression vector containing a mutated albumin minigene of Nagase analbuminemic rats (NAR), and introduced it into cultured cells. Transient expression of the minigene mRNA was determined by RT-PCR. Three kinds of aberrant mRNAs were expressed by the non-hepatic cells COS-1, and the undifferentiated human hepatoma cells HLE, transfected with the minigene. Their predominant mRNA lacked exon H (delta H), while mRNAs lacking exons H and I, or exons G and H were less abundant. This pattern of the mRNAs was similar to that of albumin mRNAs in the liver of old NAR. In contrast, a differentiated type of human hepatoma cell line, HepG2, expressed only delta H mRNA, like young NAR. These findings indicate that the expression system of the mutated minigene in cultured hepatoma cells is useful for understanding two-exon-skipping of albumin pre-mRNA of NAR.
journal_name
Cancer Lettjournal_title
Cancer lettersauthors
Sugiyama K,Hitomi Y,Adachi H,Esumi Hdoi
10.1016/0304-3835(94)90323-9subject
Has Abstractpub_date
1994-08-15 00:00:00pages
221-7issue
1-2eissn
0304-3835issn
1872-7980pii
0304-3835(94)90323-9journal_volume
83pub_type
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