Abstract:
:In the present work we investigated the in vivo regulation of the mucin gene MUC2, which is overexpressed in all mucinous colorectal carcinomas. The inhibition of methylation by 5-azadeoxycytidine induces de novo expression of MUC2 in the colon carcinoma cell line COLO 205. The expression is retained in xenograft tissue and the cells give rise to MUC2-expressing tumours in nude mice. The strong expression of MUC2 in the normal human goblet cells and in the tissue of human mucinous colorectal carcinomas is associated with the average methylation of about 50% at every investigated CpG site of the MUC2 promoter. In contrast, MUC2 promoter in the non-expressing normal columnar cells and in the non-mucinous carcinoma tissue is methylated to nearly 100%. These data show that (i) low methylation of MUC2 promoter is associated with MUC2 expression in vivo and (ii) the pattern of MUC2 promoter methylation in the normal goblet or columnar cells most closely resembles that in mucinous or non-mucinous colorectal carcinomas, respectively. They indicate that MUC2 expression in vivo is regulated by promoter methylation and support the hypothesis that cells with goblet-like differentiation give rise to mucinous colonic carcinomas.
journal_name
Cancer Lettjournal_title
Cancer lettersauthors
Gratchev A,Siedow A,Bumke-Vogt C,Hummel M,Foss HD,Hanski ML,Kobalz U,Mann B,Lammert H,Mansmann U,Stein H,Riecken EO,Hanski Cdoi
10.1016/s0304-3835(01)00498-0keywords:
subject
Has Abstractpub_date
2001-07-10 00:00:00pages
71-80issue
1eissn
0304-3835issn
1872-7980pii
S0304383501004980journal_volume
168pub_type
杂志文章相关文献
CANCER LETTERS文献大全abstract::Accumulating evidence suggested that the irreversible tyrosine kinase inhibitors (TKIs) have potential to override the acquired resistance to target-based therapies. Herein, we reported IC-4 as a novel irreversible TKI for epidermal growth factor receptor (EGFR). IC-4 potentially suppressed proliferation, induced apop...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2013.07.005
更新日期:2013-10-28 00:00:00
abstract::Azelaic acid has been shown to inhibit thioredoxin reductase (TR) at the surface of guinea pig and human skin, on cultures of human keratinocytes, melanocytes, melanoma cells, murine melanoma cells (Cloudman S91), and on purified enzymes from Escherichia coli, rat liver, and human melanoma. Human melanoma cells are mo...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(87)90023-1
更新日期:1987-09-01 00:00:00
abstract::The aim of this study was to clarify candidate peptides for peptide-based specific immunotherapy of patients with oral squamous cell carcinoma (SCC). Thirteen peptides were examined for in vitro induction of peptide-specific CD8(+) T lymphocyte (CD8(+)TL) activity in peripheral blood mononuclear cells from 35 patients...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2012.02.016
更新日期:2012-09-01 00:00:00
abstract::scFv fragments of a monoclonal antibody that penetrates living cells and localizes in nuclei were designed as fusion proteins with C-terminal p53 peptides and tested for restoring p53 function in p53 mutant cancer cells. scFv fragments transported a 30-mer C-terminal peptide of p53 into cancer cells and induced cellul...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(03)00151-4
更新日期:2003-06-10 00:00:00
abstract::Costunolide (CT), a sesquiterpene lactone constituent isolated from Saussurea lappa (Compositae), exerted an antiangiogenic effect. CT selectively inhibited the endothelial cell proliferation induced by vascular endothelial growth factor (VEGF). Further, CT was also found to inhibit the VEGF-induced chemotaxis of huma...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(02)00361-0
更新日期:2002-12-10 00:00:00
abstract::Dysregulation of circular RNA Foxo3 (circFoxo3) has been reported to be involved in breast cancer and non-small lung cancer progression. However, little is known about the role of circFoxo3 in prostate cancer, which the present study seeks to investigate. CircFoxo3 expression was analyzed in 22 low-grade prostate canc...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2019.10.006
更新日期:2020-01-01 00:00:00
abstract::Photodynamic action has been advocated as an alternative treatment of tumors but the most common used dyes, hematoporphyrin derivatives, are substrate for P-glycoprotein. This study investigated the MDR-reverting properties of methylene blue (MB) and compared the sensitivity to its photodynamic action (PDA) in five ce...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(99)00408-5
更新日期:2000-04-14 00:00:00
abstract::Emerging evidence indicates that cancer stem cells (CSCs) are involved in tumorigenesis, tumor recurrence, and therapeutic resistance in hepatocellular carcinoma (HCC). However, the mechanisms underlying HCC CSC regulation remain largely unknown. Here we report our analysis of 97 paraffin-embedded HCC tumor specimens....
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2019.02.040
更新日期:2019-05-28 00:00:00
abstract::Pancreatic ductal adenocarcinoma (PDAC) is highly aggressive disease and current treatment regimens fail to effectively cure PDAC. Development of resistance to current therapy is one of the key reasons for this outcome. Nimbolide (NL), a triterpenoid obtained from Azadirachta indica, exhibits anticancer properties in ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2017.10.029
更新日期:2018-01-28 00:00:00
abstract::The insulin-like growth factor (IGF) family of ligands, binding proteins and receptors is an important growth factor system involved in both the development of the organism and the maintenance of normal function of many cells of the body. The system also has powerful anti-apoptotic effects. More recently, evidence has...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/s0304-3835(03)00159-9
更新日期:2003-06-10 00:00:00
abstract::The overexpression of the MDM2 oncoprotein frequently occurs in hepatocellular carcinoma (HCC). Small molecules that inhibit MDM2-p53 binding show efficacy against p53 wild-type HCC, but most patients have p53-mutant tumors and intrinsic resistance to such MDM2 inhibitors. We have recently discovered that the NFAT1 tr...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2019.114429
更新日期:2019-09-10 00:00:00
abstract::Cytotoxic effects of 3-deazaguanosine (3-DGUO) result from the inhibition of DNA synthesis and incorporation of the drug into DNA. Synergistic antiproliferative effects of a combination of 3-deazaguanosine and 2-beta-D-ribofuranosylthiazole-4-carboxamide, a potent inhibitor of inosine monophosphate dehydrogenase, was ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(87)90061-9
更新日期:1987-04-01 00:00:00
abstract::NAD(P)H: quinone oxidoreductase 1 (NQO1) is an antioxidant enzyme which is associated with poor prognosis in human breast, colon, lung and liver cancers. However, the molecular mechanisms underlying the pro-tumorigenic function of NQO1 remains unclear. This study investigated the function of NQO1 in the context of hep...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2019.02.053
更新日期:2019-06-01 00:00:00
abstract::Cancer stem cells (CSCs) are involved in tumorigenesis and progression of prostate cancer (PCa). Conventional anticancer therapeutics failed to eradicate CSCs, which may eventually lead to the disease relapse and metastasis. Therefore, targeting prostate CSCs may be an ideal strategy to cure PCa. Genistein is a major ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2012.03.037
更新日期:2012-10-01 00:00:00
abstract::We planned to determine the relationship between angiogenesis and p53 mutational status in advanced-stage epithelial ovarian cancer. Using 190 tumor samples from patients with stage III and IV ovarian cancer we performed p53 sequencing, immunohistochemistry, and CD31 microvessel density (MVD) determination. MVD was el...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2012.12.016
更新日期:2013-04-30 00:00:00
abstract::Present studies show the in vitro addition of glutathione (GSH) can significantly alter selenite induced growth inhibition of mammary tumor cell line 13 (CMT-13). Preincubation with 100 microM GSH for 24 h partially prevented the growth inhibition caused by 12.6 microM selenite. Pre-incubation with 100 microM GSH for ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(91)90213-2
更新日期:1991-05-01 00:00:00
abstract::The ability of the cytosolic arylamine oxidase to convert structurally diverse aromatic and heterocyclic amines to mutagens in the Ames test was investigated using hepatic cytosol from Aroclor 1254-treated rats as the activation system. Using this system, only amino compounds containing at least three fused aromatic r...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(98)00037-8
更新日期:1998-05-15 00:00:00
abstract::14-3-3 sigma is a negative regulator of the cell cycle and contributes to G2 arrest. Thus far, the lack of its expression due to hypermethylation of the CpG islands has been reported in some carcinomas. In this study, we investigated the expression of 14-3-3 sigma in thyroid neoplasms by means of immunohistochemistry ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(03)00282-9
更新日期:2003-10-28 00:00:00
abstract::Cancer stem cells (CSCs), which comprise a small proportion of total cancer cells, have special capacities for self-renewal, differentiation and tumor formation. Currently, CSCs are regarded as the major cause of the failure in anticancer therapy, such as chemoresistance and/or radioresistance, tumor recurrence and me...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/j.canlet.2017.02.012
更新日期:2017-05-01 00:00:00
abstract::To address the possible involvement of hyperinsulinemia in breast cancer development, we have examined the susceptibility of lean and obese Zucker rats to N-methyl-N-nitrosourea (MNU)-induced mammary cancer. Fifty-day-old female lean or obese Zucker rats received intraperitoneal (i.p.) injections of 37.5 or 20 mg/kg M...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(00)00635-2
更新日期:2001-01-26 00:00:00
abstract::By comparing protein profiles of nasopharyngeal carcinoma HONE1 cells to transformed nasopharyngeal epithelial NP 69 cells, several clusters of differentially expressed proteins were identified. The increased expression of cytokeratin 8 (CK8) and pyruvate kinase M2 was a common feature in four NPC cell lines compared ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2008.02.015
更新日期:2008-07-08 00:00:00
abstract::Pancreatic cancer is one of the most aggressive malignancies and has been considered poorly immunogenic for decades. However, this characterization might be over-simplistic. A more sophisticated approach is needed in order to develop new treatment strategies. In this review, we will focus on T cell exhaustion as a phe...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/j.canlet.2016.02.057
更新日期:2016-10-10 00:00:00
abstract::The level of GSH in ovarian carcinoma cells which were sensitive and resistant to cisplatin was serially determined following tumor removal from the animals, in addition, activities of GSH-reductase and GSH-S-transferase were assessed. The GSH level in the resistant line (O-342/DDP) was almost twice as high as that in...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(89)90132-8
更新日期:1989-08-01 00:00:00
abstract::Experimental data were provided to demonstrate the inhibitory effects of retinoids (retinal, retinoic acid, retinyl acetate and retinyl palmitate) and carotenoids (beta-carotene, lycopene and crocetin) on the growth and development of the C-6 glioma cells inoculated in rats. In the pretreatment experiments, most of th...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(89)90050-5
更新日期:1989-11-30 00:00:00
abstract::Soil samples were collected from the ground under the plants of Euphorbiaceae and Thymelaeaceae known to possess Epstein-Barr virus-activating diterpene esters. In a test system, the ether extracts of such soil samples at a concentration of 20 micrograms/ml induced Epstein-Barr virus early antigen in approximately 5-2...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(83)90144-1
更新日期:1983-06-01 00:00:00
abstract::Liposarcoma is one of the most common soft tissue sarcomas in adults. Recognized histological subtypes include well differentiated/dedifferentiated liposarcoma (WD/DDLS), myxoid liposarcoma (MLS) and pleomorphic liposarcoma. Currently, there are no proper subtype-specific treatments due to the genetic, histological an...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2015.07.025
更新日期:2015-10-28 00:00:00
abstract::We studied the influence of the vitamins retinol acetate, alpha-tocopherol acetate and thiamine chloride; the antioxidant sodium selenite and an inhibitor of polyamine biosynthesis, alpha-difluoromethylornithine, on the offspring of transplacental carcinogenesis by ethylnitrosourea in rats. Ethylnitrosourea was given ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(91)90225-7
更新日期:1991-11-01 00:00:00
abstract::Microtubule targeting based therapies have revolutionized cancer treatment; however, resistance and side effects remain a major limitation. Therefore, novel strategies that can overcome these limitations are urgently needed. We made a novel discovery that fisetin, a hydroxyflavone, is a microtubule stabilizing agent. ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2015.07.030
更新日期:2015-10-28 00:00:00
abstract::Exosomes are structurally and functionally pleiotropic nano-sized (~30-150 nm in diameter) extracellular vesicles (EVs) with endosomal origin. These vesicles are secreted by almost all cells and play a significant role in intercellular communication and bio-waste disposal. To a great extent, exosomes represent biologi...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/j.canlet.2020.05.018
更新日期:2020-09-28 00:00:00
abstract::Tumor tissues are chronically exposed to hypoxia owing to aberrant vascularity. Hypoxia induces metabolic alterations in cancer, thereby promoting aggressive malignancy and metastasis. While previous efforts largely focused on adaptive responses in glucose and glutamine metabolism, recent studies have begun to yield i...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2020.06.013
更新日期:2020-11-28 00:00:00