Abstract:
:The role of polyamines (PA) synthesis in NMDA receptor-mediated 45Ca2+ fluxes and norepinephrine release was studied in rat hippocampal synaptosomes. NMDA (50 microM) caused a sharp (> 2-fold) transient increase in PA synthesis regulating enzyme, ornithine decarboxylase (ODC) activity with concomitant elevation in PA levels in the order putrescine > spermidine > spermine. ODC inhibitor, alpha-difluoromethylornithine (DFMO), and NMDA antagonist, 2-amino-5-phosphonovaleric acid (D-AP5), both blocked increases in ODC activity and PA levels. Activation of NMDA receptors induced a sharp (3 to 4-fold) and quick (15 seconds) increase in 45Ca2+ uptake by synaptosomes within 15 seconds of exposure at 37 degrees C. The efflux of 45Ca2+ and 3H-norepinephrine (NE) release at 22 degrees C from pre-loaded synaptosomes was also significantly (2 to 4-fold) enhanced by NMDA within 15 seconds. These NMDA receptor-mediated effects on calcium fluxes and NE release were blocked by NMDA receptor-antagonists (DAP-5 and MK-801) and PA synthesis inhibitor, DFMO and the DFMO inhibition nullified by exogenous putrescine. These observations establish that ODC/PA cascade play an important role in transduction of excitatory amino acid mediated signals at NMDA receptors.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Siddiqui F,Iqbal Zdoi
10.1007/BF00972471subject
Has Abstractpub_date
1994-11-01 00:00:00pages
1421-9issue
11eissn
0364-3190issn
1573-6903journal_volume
19pub_type
杂志文章abstract::Single prolonged stress (SPS) rats is a rodent model of post traumatic stress disorder (PTSD). Abnormal hippocampal morphology and function were found in the PTSD patients. Our previous study has shown that SPS induce loss of hippocampal neurons. But the effects of SPS on glial cells in the hippocampus have not been e...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1549-6
更新日期:2015-05-01 00:00:00
abstract::Selective and limited proteolysis is a key step in the post-translational modification of peptide hormone precursors. This process appears to involve a proteolytic machinery including highly specific endoproteases. Some of the enzyme systems possibly involved in the processing of pro-neuropeptides will be described an...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/BF00966318
更新日期:1987-10-01 00:00:00
abstract::Alzheimer's disease (AD) is a devastative neurodegenerative disorder with complex etiology. Apoptosis, a biological process that plays an essential role in normal physiology to oust a few cells and contribute to the normal growth, when impaired or influenced by various factors such as Bcl2, Bax, caspases, amyloid beta...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-014-1454-4
更新日期:2014-12-01 00:00:00
abstract::The MAO inhibitor phenelzine (PLZ) at a dose of 25 mg/kg does not affect the behavior of rats. In contrast, the equivalent dose of a deuterated analog (alpha, alpha, beta, beta-tetradeutero-PLZ, d4PLZ) elicits a biphasic behavioral syndrome in rats. In an attempt to correlate changes in cerebral monoamines with behavi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00973322
更新日期:1988-02-01 00:00:00
abstract::Sonic hedgehog (Shh), a secreted glycoprotein factor, can activate the Shh pathway, which has been implicated in neuronal polarization involving neurite outgrowth. However, little evidence is available about the effect of Shh on neurite outgrowth in primary cortical neurons and its potential mechanism. Here, we reveal...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1736-5
更新日期:2016-04-01 00:00:00
abstract::The effect of castration on the levels of brain monoamines and their metabolites has been investigated in rats which became or did not become muricidal following long-term isolation. Fourteen brain areas were explored: olfactory bulbs (OB), olfactory tubercles (OT), septum (Se), striatum (Sr), amygdala (A), thalamus (...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00967251
更新日期:1993-04-01 00:00:00
abstract::Cloned fragments of members of the Drosophila and mouse major heat shock (hsp70) gene family were used to demonstrate that homologous sequences are present in the rabbit genome. After a physiologically relevant increase in body temperature of 3 degrees C, transcription of inducible hsp70 genes is detected in both the ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964846
更新日期:1985-09-01 00:00:00
abstract::The specific molecular events that underlie the age-related loss of cognitive function are poorly understood. Although not experimentally substantiated, age-dependent neuronal loss has long been considered central to age-related cognitive decline. More recently, age-related changes in brain white matter have taken pre...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-007-9341-x
更新日期:2007-12-01 00:00:00
abstract::The monoclonal antibody 14E immunocytochemically stains the nuclear membrane of oligodendrocytes but not myelin in tissue sections of adult normal human white matter. The nuclear membranes of Schwann cells in human peripheral nerve and cerebellar Bergmann glia were also visualized with this antibody. In actively demye...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00993270
更新日期:1992-09-01 00:00:00
abstract::Pathophysiological concentrations of branched chain keto-acids (BCKAs), such as those that occur in maple syrup urine disease, inhibit oxygen consumption in liver homogenates and brain slices and the enzymatic activity of alpha-ketoglutarate- and pyruvate dehydrogenase complexes. Consistent with previous work, studies...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9291-3
更新日期:2007-04-01 00:00:00
abstract::Parkinson's disease (PD) is a neurodegenerative disease, with genetics and environment contributing to the disease onset. The limited pathological cognize of the disease restrained the approaches to improve the clinical treatment. Recently, studies showed that endoplasmic reticulum (ER) stress played an important role...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-1922-0
更新日期:2016-08-01 00:00:00
abstract::Bilateral lesion of the nucleus basalis with ibotenic acid infusions in young and aged rats results in the degeneration of cholinergic neurons which innervate the cortex. As expected, high-affinity uptake of choline was decreased in the frontal cortex subsequent to the lesion. Twenty one days after surgery there was a...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00974576
更新日期:1992-04-01 00:00:00
abstract::Recent evidence indicates that mechanisms involved in reward and mechanisms involved in learning interact, in that reward includes learning processes and learning includes reward processes. In spite of such interactions, reward and learning represent distinct functions. In the present study, as part of an examination ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9343-8
更新日期:2007-10-01 00:00:00
abstract::Ingestion of ammonium induces hyperammonemia which increases tubulin content in cerebrum but not in cerebellum. We have dissected 11 discrete areas of cerebrum and quantified the tubulin content in control and hyperammonemic rats. An heterogeneity in the induction of tubulin is shown. The areas more affected are ventr...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965516
更新日期:1989-12-01 00:00:00
abstract::Incomplete ischemia of the spinal cord was produced in dogs by 40 min occlusion of the abdominal aorta that was followed by 5-40 min of recirculation. Amino acid incorporation into ribosomes in vitro in the presence of venous blood sera was estimated. The most significant reduction in incorporation was produced by ser...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965210
更新日期:1986-06-01 00:00:00
abstract::The antibodies against omega-conotoxin GVIA (omega-CTX GVIA; N-type voltage-dependent calcium channel [VDCC] blocker) and B1Nt (N-terminal segment [residues 1-13] of BI alpha1 subunits of VDCCs) were prepared, and the selectivity for each antigen omega-CTX GVIA and B1Nt was investigated. For the antigen selectivity of...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1023208103438
更新日期:2003-06-01 00:00:00
abstract::The participation of voltage-sensitive Na+ channels (VSSC) on the changes on internal (i) Na+, K+, Ca2+, and on DA, Glu, and GABA release caused by different concentrations of 4-AP was investigated in striatum synaptosomes. TTX, which abolished the increase in Na(i) (as determined with SBFI), induced by 0.1 mM 4-AP on...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/b:nere.0000013737.17288.ce
更新日期:2004-02-01 00:00:00
abstract::We have synthesized the tertiary amines of pyridostigmine and neostigmine, 3-pyridinol dimethylcarbamate (norpyridostigmine) and 3-dimethylaminophenol dimethylcarbamate (norneostigmine) respectively, and we have tested their abilities to cross the blood-brain barrier and inhibit mouse brain AChE activity. The in vivo ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00973747
更新日期:1990-06-01 00:00:00
abstract::Isozyme patterns of cholinesterase (ChE) from heart, tongue, and skeletal muscle of normal and dystrophic hamsters are presented. Two principal bands, bands 1 and 2, were evaluated. Band 1 migrates faster towards the anode than does band 2. While bands 1 and 2 stain for AChE and were found in control muscles, only ban...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964958
更新日期:1980-12-01 00:00:00
abstract::Protein kinase C has recently attracted considerable attention because of its importance in the control of cell division, cell differentiation, and signal transduction across the cell membrane. The activity of this enzyme is altered by several lipids such as diacylglycerol, free fatty acids, lipoxins, gangliosides, an...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/BF00973288
更新日期:1988-06-01 00:00:00
abstract::The subcellular distribution of Ca2+/calmodulin-stimulated adenylate cyclase activity was studied in comparison with that of guanine nucleotide-stimulated cyclase activity. The distributions of these activities were similar among the crude fractions but differed among the purified subsynaptosomal fractions. The specif...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965018
更新日期:1986-03-01 00:00:00
abstract::Using the mouse maximal electroshock test, the reference model of tonic-clonic seizures, the aim of the present study was to determine the type of interaction between mexiletine (a class IB antiarrhythmic drug) and classical antiepileptics: valproate, carbamazepine, phenytoin, and phenobarbital. Isobolographic analysi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1812-x
更新日期:2016-05-01 00:00:00
abstract::The present study reports inhibition of taurine uptake in the rat retina in vitro to 49% of control by 1 mM guanidinoethyl sulfonate. No efflux of preloaded [14C]taurine was observed by incubation in the presence of guanidinoethyl sulfonate. The in vivo depletion of retinal taurine by guanidinoethyl sulfonate treatmen...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964157
更新日期:1983-12-01 00:00:00
abstract::Brain regional 5-HT (5-hydroxytryptamine) and 5-HIAA (5-hydroxyindole-3-acetic acid) concentrations in male LE rats at three times were measured by a fluorescence method, to evaluate effects of intracranial surgery and administration of melatonin on the changes in these compounds during the first part of the dark phas...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965929
更新日期:1989-10-01 00:00:00
abstract::Blood-brain barrier formed by brain capillary endothelial cells, being in contact with astrocytes endfeet and pericytes, separates extracellular fluid from plasma. Supply of necessary nutrients and removal of certain metabolites takes place due to the activity of transporting proteins from ABC (ATP binding cassette) a...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-016-2030-x
更新日期:2017-03-01 00:00:00
abstract::Rat posterior eyecups containing the retina were prepared, loaded with [(3)H]glycine and superfused in order to determine its release originated from glycinergic amacrine cells and/or glial cells. Deprivation of oxygen and glucose from the Krebs-bicarbonate buffer used for superfusion evoked a marked increase of [(3)H...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1713-z
更新日期:2016-02-01 00:00:00
abstract::Spinal cord injury (SCI) is a type of long-term disability with a high morbidity rate in clinical settings for which there is no effective clinical treatment to date. Usually, lithium is used as a popular mood stabilizer. Recently, growing evidence has shown that lithium has clear neuroprotective effects after SCI, an...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-018-2488-9
更新日期:2018-04-01 00:00:00
abstract::Serotonin-synthesizing raphé/parapyramidal neurons (5-HT neurons) may function as sympathetic premotor neurons regulating sympathetic outflow to the cutaneous vascular bed. In the present study a genetically engineered pseudorabies virus (PRV) expressing green fluorescent protein (GFP) was injected into the rat tail. ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-005-9018-2
更新日期:2006-02-01 00:00:00
abstract::Evidence has suggested that cerebrospinal fluid-contacting nucleus (CSF-contacting nucleus) is correlated with the development and recurrence of pain. A recent research showed that the CSF-contacting nucleus acts as a component of the descending 5-hydroxytryptamine (5-HT) system and plays a role in descending pain inh...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-1880-6
更新日期:2016-07-01 00:00:00
abstract::Protein kinase A (PKA) plays a crucial role in tau hyperphosphorylation, an early event of Alzheimer disease (AD), and 17beta-estradiol replacement in aging women forestalls the onset of AD. However, the role of estradiol in PKA-induced tau hyperphosphorylation is not known. Here, we investigated the effect of 17beta-...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-008-9638-4
更新日期:2008-09-01 00:00:00