Abstract：
:The mycotoxin ochratoxin A (OTA) and its metabolite ochratoxin alpha (OT-alpha) were investigated, to examine their potency to induce sister chromatid exchanges (SCE) in cultured porcine urinary bladder epithelial cells (PUBEC) (primary culture). Serum-free cultured PUBEC were incubated for 5 h with either OTA or OT-alpha, respectively, and subsequently cultured in the presence of 5-bromo-2-deoxyuridine (BrdU). After two cell cycles, mitosis was inhibited by the colchicine derivative Colcemid, cells were fixed and chromosomes were prepared for SCE analysis. For OTA, a dose-dependent increase in SCE frequency was measured in concentrations between 100 pM and 100 nM OTA. At 100 nM OTA, SCE frequency increased by about 41%, compared to the base SCE level (7.27 SCEs per chromosome set, solvent control). Higher concentrations of OTA were cytotoxic. The metabolite OT-alpha also increased SCE frequency, but at higher concentrations. At a concentration of 10 microM OT-alpha, an increase of about 55% was detected. OT-alpha showed no cytotoxic effect. These results indicate that OTA is genotoxic in this in vitro system, which represents the urinary bladder epithelium, a target organ of OTA in vivo. It could also be shown that OT-alpha, which is said to be non-toxic, is genotoxic in this assay at higher concentrations.
journal_name
Arch Toxicoljournal_title
Archives of toxicologyauthors
Föllmann W,Hillebrand IE,Creppy EE,Bolt HMdoi
10.1007/s002040050171subject
Has Abstractpub_date
1995-01-01 00:00:00pages
280-6issue
4eissn
0340-5761issn
1432-0738journal_volume
69pub_type
杂志文章abstract：:Tea (Camellia sinensis, Theaceace), a popular beverage consumed world-wide, has been studied for its preventive effects against cancer as well as cardiovascular, neurodegenerative, and other diseases. Most of the proposed beneficial effects have been attributed to the polyphenolic compounds in tea, but the nature of t...
journal_title：Archives of toxicology
pub_type： 杂志文章,评审
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abstract：:Quantitative structure-activity relationships (QSAR) have suggested the importance of hydrogen bonding in relation to activation of the sensory irritant receptor by nonreactive volatile organic chemicals. To investigate this possibility further, three model compounds with different hydrogen bond acidity, trifluoroetha...
journal_title：Archives of toxicology
pub_type： 杂志文章
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更新日期：1996-01-01 00:00:00
abstract：:Blooms of cyanobacteria have been documented throughout history, all over the world. Mass populations of these organisms typically present hazards to human health and are known for the production of a wide range of highly toxic metabolites-cyanotoxins, of which among the most common and most investigated are the micro...
journal_title：Archives of toxicology
pub_type： 杂志文章,评审
doi：10.1007/s00204-016-1921-6
更新日期：2017-02-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-015-1489-6
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pub_type： 杂志文章
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更新日期：2003-02-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-006-0138-5
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-019-02547-x
更新日期：2019-10-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-019-02549-9
更新日期：2019-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00204-009-0403-5
更新日期：2009-06-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-019-02637-w
更新日期：2020-02-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-017-2002-1
更新日期：2017-08-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章,评审
doi：10.1007/s00204-016-1767-y
更新日期：2016-10-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-008-0288-8
更新日期：2008-10-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章,评审
doi：10.1007/s002040000109
更新日期：2000-07-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF01234482
更新日期：1983-12-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-019-02454-1
更新日期：2019-06-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF00263954
更新日期：1986-05-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-018-2306-9
更新日期：2018-11-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s002040050253
更新日期：1996-01-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章,评审
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-006-0109-x
更新日期：2006-12-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-015-1466-0
更新日期：2016-03-01 00:00:00
abstract：:Female C57/BL/6 x DBA/2 hybrid mice were exposed to two concentrations (270 and 135 ppm) of tetrachloroethylene (PER) in inhalation chambers for 6 h/day, 5 days per week, up to 11.5 weeks (270 ppm) and 7.5 weeks (135 ppm), respectively, followed by a 3-week exposure-free period. In the peripheral blood a reduction of ...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF01974021
更新日期：1992-01-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-013-1030-8
更新日期：2013-08-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF02342509
更新日期：1992-01-01 00:00:00
abstract：:3H-soman (specific activity 10 Ci/mMol), a potent irreversible cholinesterase inhibitor, was administered IV to mice in a dose of one LD-50, which corresponds to 0.25 mCi/mouse. Animals were sacrificed at 5 min, 2 h and 24 h, and whole body autoradiography was performed. High levels of radioactivity in lung and skin w...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF00292616
更新日期：1985-10-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
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更新日期：2006-01-01 00:00:00
abstract：:The effects of a single dose of ethanol on the metabolism and toxicity of chloroform administered to rats per os (p.o.), intraperitoneally (i.p.), or by inhalation (inh) at different doses were investigated. Rats that had been given either ethanol (2 g/kg) or vehicle (water) alone at 4 p.m. on the previous day were ch...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s002040050131
更新日期：1994-01-01 00:00:00
abstract：:The effect of acetone pretreatment (5% in drinking water for 10 days on rat liver metabolism and toxicity of thiobenzamide (TB) was investigated. Hepatic microsomes from acetone-pretreated rats showed a significant increase of TB-S-oxidation rate which, on the basis of selective thermal inactivation of FAD-containing ...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF01974397
更新日期：1990-01-01 00:00:00
abstract：:With the development of nanotechnology, myriad types of novel materials have been discovered at the nanoscale, among which the most interesting material is graphene. However, the toxicity data available on graphene are extremely limited. In this study, we explored toxic response of commercially available graphene nano...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-014-1303-x
更新日期：2015-09-01 00:00:00
