Diagnosis of genital infection caused by human papillomavirus using in situ hybridisation: the importance of the size of the biopsy specimen.

Abstract:

AIM:To determine the size of a cervical biopsy specimen with human papillomavirus (HPV) infection required to enable in situ hybridisation to be carried out with a guarantee of a reliable result. METHODS:In situ hybridisation was carried out in 142 cervical uterine biopsy specimens classified histologically as low grade and high grade squamous intraepithelial lesions. Epithelial length at the level of the basal membrane was measured by image analysis. The specimens were divided into 10 groups based on epithelial length. RESULTS:Of the biopsy specimens, 61.2% were HPV positive. In specimens with an epithelial length below 5 mm 31.9% were HPV positive; in those between 5 and 9 mm in length 67.5% were HPV positive; and in those greater than 9 mm in length 81.8% were positive for HPV. For low grade squamous intraepithelial lesions (n = 90), 68.4% of specimens with an epithelial length greater than 5 mm were HPV positive. For high grade squamous intraepithelial lesions (n = 52), 86.8% of specimens with an epithelial length greater than 5 mm were HPV positive. CONCLUSIONS:For a diagnosis of HPV infection using in situ hybridisation, the minimum length of epithelium in a cervical biopsy specimen should be 5 mm. For high grade squamous intraepithelial lesions, specimens over 5 mm in length are suitable. For low grade squamous intraepithelial lesions, to minimise the number of false negative results, the ideal minimum length is 10 mm.

journal_name

J Clin Pathol

authors

Gómez F,Corcuera MT,Muñoz E,Roldan M,Alonso MJ,Abad M,Paz JI,López-Bravo A

doi

10.1136/jcp.48.1.57

subject

Has Abstract

pub_date

1995-01-01 00:00:00

pages

57-8

issue

1

eissn

0021-9746

issn

1472-4146

journal_volume

48

pub_type

杂志文章
  • A pilot study of the reference values for the commoner haematological and biochemical parameters in Saudi nationals.

    abstract::A study of 16 haematological and 24 biochemical parameters in healthy, adult, male, volunteer Saudi blood donors was made, and enabled a preliminary determination of the reference values for these parameters to be made for Saudi Arabia. Statistically significant low means were observed for all the haematological param...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.35.1.69

    authors: Scott TG

    更新日期:1982-01-01 00:00:00

  • Cerebral ventriculitis associated with Achromobacter xylosoxidans.

    abstract::Six patients in the neurosurgical ward of Fukushima Medical College Hospital suffering from ventriculitis due to Achromobacter xylosoxidans infection had undergone craniotomy or cranial trepanation before the infection. The strains of A. xylosoxidans isolated from the patients were resistant to streptomycin, ampicilli...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.31.2.156

    authors: Shigeta S,Yasunaga Y,Honzumi K,Okamura H,Kumata R,Endo S

    更新日期:1978-02-01 00:00:00

  • Haemoglobin Bonn in a Chinese family as a cause of spurious hypoxaemia measured by pulse oximetry.

    abstract::Haemoglobin (Hb) Bonn is a newly described benign Hb variant that causes falsely depressed oxygen saturation as measured by pulse oximetry. It was found to be associated with mild haemolysis. Since its first report in a German family, no further cases have been documented in the literature. We report the first Chinese...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.2010.078147

    authors: So CC,Chan AY,Chow EY,Lin SY,Yip SF,Szeto SC,Chan LC

    更新日期:2010-10-01 00:00:00

  • Thymidine phosphorylase expression in progression of cervical cancer: correlation with microvessel count, proliferating cell nuclear antigen, and apoptosis.

    abstract:AIMS:To determine how epithelial and stromal thymidine phosphorylase expression affects angiogenesis, rapid tumour growth, and decreased apoptotic activity in cervical cancer at varying stages of progression. METHODS:Epithelial and stromal thymidine phosphorylase expression, the microvessel count (reflected by factor ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.52.8.598

    authors: Fujiwaki R,Hata K,Iida K,Maede Y,Koike M,Miyazaki K

    更新日期:1999-08-01 00:00:00

  • Coagulase-negative staphylococci and micrococci in urinary tract infections.

    abstract::One hundred catalase-positive, coagulase-negative, Gram-positive cocci isolated in significant numbers from the urine of patients with urinary tract infections, provisionally subdivided by their sensitivity to nonoviocin, were classified according to a slightly model version of Baird-Parker's schemes (1965 and 1972). ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.28.4.270

    authors: Meers PD,Whyte W,Sandys G

    更新日期:1975-04-01 00:00:00

  • Routine application of the nitroblue tetrazolium test in the clinical laboratory.

    abstract::A modified nitroblue tetrazolium test (NBT) is described which is suitable for routine application in the haematology or bacteriology laboratory and which provides a rapid aid to the diagnosis of bacterial infection. Hitherto published methods have recommended the use of heparinized whole blood samples for the perform...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.26.1.52

    authors: Gordon AM,Rowan RM,Brown T,Carson HG

    更新日期:1973-01-01 00:00:00

  • Viral exposure and abnormal liver function in haemophilia.

    abstract::Several studies have recently documented the presence of persistently abnormal liver function tests in asymptomatic haemophiliacs. While the aetiology is unknown it is possible that repeated exposure to agents transmitted in blood products may be important. This study has attempted to determine the prevalence of viral...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.32.4.377

    authors: McVerry BA,Ross MG,Knowles WA,Voke J

    更新日期:1979-04-01 00:00:00

  • PYOCINE-TYPING OF HOSPITAL STRAINS OF PSEUDOMONAS PYOCYANEA.

    abstract::A method of typing Pseudomonas pyocyanea is described which makes use of the property of bacteriocine production. The method was applied in an epidemiological study of Ps. pyocyanea infection in a general hospital. It was found that the majority of strains believed on epidemiological grounds to be of common origin wer...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.17.3.236

    authors: DARRELL JH,WAHBA AH

    更新日期:1964-05-01 00:00:00

  • Hashimoto's thyroiditis associated with urticaria and angio-oedema: disappearance of cutaneous and mucosal manifestations after thyroidectomy.

    abstract::A 60 year old woman affected by Hashimoto's thyroiditis presented with a history of recurring episodes of urticaria and angio-oedema. Clinical and laboratory evaluation of the patient excluded allergy to external agents, hereditary angio-oedema, and occult infections. A pathogenic relation between Hashimoto's thyroidi...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.50.3.254

    authors: Amoroso A,Garzia P,Pasquarelli C,Sportelli G,Afeltra A

    更新日期:1997-03-01 00:00:00

  • Concurrent mutations in other epigenetic modulators portend better prognosis in BCOR-mutated myelodysplastic syndrome.

    abstract:INTRODUCTION:The role of single mutations has been extensively studied myelodysplastic syndromes (MDS), but the impact of genetic aberrations in the context of other mutations is less well understood. BCOR is an epigenetic transcriptional corepressor. In MDS, BCOR mutations are rare and certain mutations are associated...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jclinpath-2019-206132

    authors: Badaat I,Mirza S,Padron E,Sallman D,Komrokji R,Song J,Hussaini MO

    更新日期:2020-04-01 00:00:00

  • Serological cross reaction between legionella and campylobacter in the rapid microagglutination test.

    abstract:AIMS:To investigate the serological cross reaction between legionella and campylobacter using the rapid microagglutination test (RMAT). METHODS:Serum samples from 49 patients with campylobacter infection were tested for legionella antibodies using the indirect fluorescent antibody test (IFAT) and the RMAT. Serum sampl...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.49.7.584

    authors: Boswell TC

    更新日期:1996-07-01 00:00:00

  • Changes in alveolar macrophage, monocyte, and neutrophil cell profiles after smoke inhalation injury.

    abstract::Thirty two fire victims with smoke inhalation, with or without burns, and 26 control subjects had bronchoalveolar lavage performed. Cell yields and differential cell counts were assessed. All patients and controls were cigarette smokers. Patients with smoke inhalation (SI) injury generally showed higher total bronchoa...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.43.1.43

    authors: Riyami BM,Tree R,Kinsella J,Clark CJ,Reid WH,Campbell D,Gemmell CG

    更新日期:1990-01-01 00:00:00

  • Characteristics of some co-trimoxazole-resistant Enterobacteriaceae from infected patients.

    abstract::Two isolates of Proteus mirabilis and four of Esch. coli which would not grow on commonly used commercial sensitivity test media are reported. These organisms appear to be resistant to co-trimoxazole, are exacting towards thymidine, and five of six were isolated from patients who had been treated with co-trimoxazole. ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.25.12.1086

    authors: Barker J,Healing D,Hutchison JG

    更新日期:1972-12-01 00:00:00

  • Reduplication cyst of appendix with mucinous carcinoma and Müllerian metaplasia: a case report.

    abstract::This report describes a case of mucinous carcinoma and Müllerian metaplasia arising within an appendiceal duplication cyst found incidentally during an emergency Caesarian section. Intestinal duplication cysts are rare and although there are occasional reports of malignant transformation, this is the first case where ...

    journal_title:Journal of clinical pathology

    pub_type: 信件

    doi:10.1136/jcp.55.7.551

    authors: Sington JD,Warren BF,Manek S

    更新日期:2002-07-01 00:00:00

  • Mucinous, gland predominant synovial sarcoma of a large peripheral nerve: a rare case closely mimicking metastatic mucinous carcinoma.

    abstract::Synovial sarcoma is a distinctive soft tissue neoplasm with monophasic and biphasic forms. It is typically a deep-seated soft tissue tumour of the extremities of young adults and occasional cases have been described in large peripheral nerves. A rare example has a predominance of the glandular component and may mimic ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.2007.053124

    authors: Weinreb I,Perez-Ordoñez B,Guha A,Kiehl TR

    更新日期:2008-05-01 00:00:00

  • Rapid identification of Candida species in oral rinse solutions by PCR.

    abstract:AIMS:To determine the sensitivity and specificity of a multiplex PCR assay for the contemporary identification of major species involved in oral candidiasis, without extraction and purification of DNA from the samples under investigation; and evaluation of this method in comparison with routine phenotypic culture ident...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.2006.040782

    authors: Liguori G,Lucariello A,Colella G,De Luca A,Marinelli P

    更新日期:2007-09-01 00:00:00

  • Isolation of Neisseria lactamicus from the nasopharynx.

    abstract::During 1971 and 1972, 71 cultures of neisseriae that attacked lactose were received by this laboratory. All strains except one from an eye swab were from the nasopharynx of healthy subjects. Nineteen similar strains from the nasopharynx were isolated in this laboratory. The characteristics of these strains were compar...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.26.6.399

    authors: Pykett AH

    更新日期:1973-06-01 00:00:00

  • A simplified economic approach to thyroid FNA cytology and surgical intervention in thyroid nodules.

    abstract:OBJECTIVE:Few studies have modelled the economics of thyroid FNA. METHODS:A simple spreadsheet economic model for delivery of thyroid fine needle aspiration (FNA) cytology is described using the UK Royal College of Pathologists' Classification for thyroid FNA which is based on The Bethesda System for Reporting Thyroid...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jclinpath-2012-201339

    authors: Poller DN,Kandaswamy P

    更新日期:2013-07-01 00:00:00

  • Application of ELISA for assessment of antiserum immunoreactivity in endocrine immunocytochemical studies.

    abstract::An indirect method of an enzyme-linked immunosorbent assay (ELISA) is described to assess the reactivity of antisera used for the identification of peptide hormone producing cells by immunocytochemistry. Compared with radioimmunoassay and immunodiffusion, the ELISA method has the advantages of simplicity and sensitivi...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.36.4.406

    authors: Nierwenhuijzen Kruseman AC

    更新日期:1983-04-01 00:00:00

  • CYTOENZYMATICAL DIFFERENTIATION OF NORMAL AND NEOPLASTIC PLASMA CELLS.

    abstract::A histochemical method is described of differentiating between normal and neoplastic cells in plasma using the activity of various enzymes characteristic of plasma cells. It was found that in both types of cell increased or diminished activity of cytoenzymes is a useful means of distinguishing between normal and abnor...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.18.3.345

    authors: SZMIGIELSKI S,LITWIN J,ZUPANSKA B

    更新日期:1965-05-01 00:00:00

  • Severe coagulation defect due to a dietary deficiency of vitamin K.

    abstract::A patient with multiple injuries developed a severe coagulation defect due to a deficiency of vitamin K. None of the usual predisposing factors was present and the patient's diet was very poor. Dietary deficiency of vitamin K and the importance of antibiotic therapy in its production are discussed. ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.30.12.1147

    authors: Colvin BT,Lloyd MJ

    更新日期:1977-12-01 00:00:00

  • Antibiotic-resistant Staphylococcus aureus in dermatology and burn wards.

    abstract::Staphylococcus aureus isolated between 1967 and 1975 from the nose and skin lesions of patients in dermatology wards and from the burns of patients in a burns unit in Birmingham showed a high incidence of multiple antibiotic-resistant strains in both environments. Over 20% of the strains isolated from patients on admi...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.30.1.40

    authors: Ayliffe GA,Green W,Livingston R,Lowbury EJ

    更新日期:1977-01-01 00:00:00

  • In situ hybridisation with digoxigenin-labelled DNA probes for detection of viral genomes.

    abstract::The applicability of a recently developed non-radioactive DNA labelling and detection method, which uses the digoxigenin (DIG) enzyme linked immunosorbent assay (ELISA) system, for the detection of viral infections in pathology specimens by in situ hybridisation, was examined. Its efficacy was compared with that of bi...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.43.10.806

    authors: Furuta Y,Shinohara T,Sano K,Meguro M,Nagashima K

    更新日期:1990-10-01 00:00:00

  • Costs of a clinical chemistry laboratory.

    abstract::The costs of a clinical chemistry laboratory in a district general hospital were studied. The system used has certain advantages over the conventional Cooper Lybrand method. The time taken by technicians to perform tests was more variable than expected and the cost of sample collection was higher than process-cost for...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.34.6.589

    authors: Stilwell JA

    更新日期:1981-06-01 00:00:00

  • Modified colorimetric ultramicro method for estimating Nefa in serum.

    abstract::A method is described for the estimation of serum free fatty acids based on the ultramicro method of Novák. The difficulty of obtaining an accurate standard graph has been overcome and the interfering effects of phospholipids have been reduced. The method is simple to perform and gives accurate, precise results in 50 ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.21.5.567

    authors: Elphick MC

    更新日期:1968-09-01 00:00:00

  • Evaluation of an enhanced pathological examination protocol for sentinel lymph nodes from patients with breast carcinoma.

    abstract::Clinical trials have shown that many patients with breast cancer with limited sentinel lymph node (SLN) metastatic disease can safely avoid axillary lymph node dissection. Ultra-staging of initially negative SLNs may not confer additional clinical benefit. Despite this, protocols of 'enhanced pathological examination'...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jclinpath-2020-206629

    authors: Murray C,Al Shehhi A,Ó'Riain C,Dunne B,Maguire A

    更新日期:2020-10-22 00:00:00

  • Unusually high incidence of tuberculosis among boys with haemophilia during an outbreak of the disease in hospital.

    abstract::Of 30 children with bleeding disorders exposed to an index tuberculosis case, six developed primary pulmonary tuberculosis and two others became positive skin reactors. There was a significant correlation between the amount of replacement treatment received and the development of evidence of tuberculous infection. Onl...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.38.10.1163

    authors: Beddall AC,Hill FG,George RH,Williams MD,Al-Rubei K

    更新日期:1985-10-01 00:00:00

  • Mucin depletion in inflammatory bowel disease.

    abstract::The mucin and gland content of 26 rectal biopsy specimens--five normal specimens, 10 from patients with ulcerative colitis, and 11 from patients with Crohn's disease--were measured using a Quantimet image analyser. There was significantly less mucin in the groups with ulcerative colitis compared with either those with...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.43.2.143

    authors: McCormick DA,Horton LW,Mee AS

    更新日期:1990-02-01 00:00:00

  • Basal cell carcinoma arising in a smallpox vaccination site.

    abstract::A case of pigmented basal cell carcinoma developing in a smallpox revaccination site is presented. Any progressive change within a smallpox vaccination scar should be thoroughly evaluated and treated appropriately after tissue diagnosis. ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.33.2.134

    authors: Rich JD,Shesol BF,Horne DW 3rd

    更新日期:1980-02-01 00:00:00

  • Formalin fixation and patterns of antineutrophil cytoplasmic antibodies.

    abstract::Recent publications have suggested that fixing neutrophils in formalin is a useful adjunct to the differentiation of antinuclear antibodies from perinuclear antineutrophil cytoplasmic antibodies. In this small comparative study of 30 sera the use of an additional slide of formalin fixed neutrophils did not add any use...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.48.1.89

    authors: Spickett GP,Broomhead V

    更新日期:1995-01-01 00:00:00