Application of ELISA for assessment of antiserum immunoreactivity in endocrine immunocytochemical studies.

Abstract:

:An indirect method of an enzyme-linked immunosorbent assay (ELISA) is described to assess the reactivity of antisera used for the identification of peptide hormone producing cells by immunocytochemistry. Compared with radioimmunoassay and immunodiffusion, the ELISA method has the advantages of simplicity and sensitivity and represents with the hormone adsorbed to a matrix a situation more or less comparable to that in tissue sections. It is concluded that specificity testing of antisera applied in endocrine immunocytochemical studies can best be achieved by application of the ELISA method in combination with appropriate tissue controls.

journal_name

J Clin Pathol

authors

Nierwenhuijzen Kruseman AC

doi

10.1136/jcp.36.4.406

subject

Has Abstract

pub_date

1983-04-01 00:00:00

pages

406-10

issue

4

eissn

0021-9746

issn

1472-4146

journal_volume

36

pub_type

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