Gas-liquid chromatography in the diagnosis of anaerobic infections: a three year experience.

Abstract:

:Nearly two thousand clinical samples were examined by direct gas-liquid chromatography over a three year period. Absence of volatile fatty acids (VFAs) in the samples correlated well with negative culture results for anaerobic bacteria. In general the presence of acetic acid alone correlated well with the presence of aerobic organisms, whereas the presence of a mixture of VFAs correlated well with the presence of anaerobic organisms, either alone or in combination with aerobes. However a proportion of such VFA-positive samples gave no growth on culture. Swabs gave comparable results to samples of pus or exudates except that a higher proportion of the former were VFA-negative but culture positive.

journal_name

J Clin Pathol

authors

Watt B,Geddes PA,Greenan OA,Napier SK,Mitchell A

doi

10.1136/jcp.35.7.709

subject

Has Abstract

pub_date

1982-07-01 00:00:00

pages

709-14

issue

7

eissn

0021-9746

issn

1472-4146

journal_volume

35

pub_type

杂志文章
  • Neisseria gonorrhoeae: a versatile pathogen.

    abstract::Neisseria gonorrhoeae is one of the most important causes of sexually transmitted disease. We do not fully understand the pathogenesis of infection with this organism, although recent improvements in immunological and molecular techniques have brought us closer to an answer. These techniques are now also being used to...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:10.1136/jcp.40.9.1088

    authors: Easmon CS,Ison CA

    更新日期:1987-09-01 00:00:00

  • Novel epitheliomesenchymal biphasic stomach tumour (gastroblastoma) in a 9-year-old: morphological, ultrastructural and immunohistochemical findings.

    abstract::Gastroblastoma is a rare gastric epitheliomesenchymal biphasic tumour composed of spindle and epithelial cells, reported by Miettinen et al in a series of three cases in 2009. All those cases arose in stomachs of young adults. Neither the epithelial nor the mesenchymal component displayed sufficient atypia to diagnose...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.2009.074104

    authors: Shin DH,Lee JH,Kang HJ,Choi KU,Kim JY,Park DY,Lee CH,Sol MY,Park JH,Kim HY,Montgomery E

    更新日期:2010-03-01 00:00:00

  • Maintenance and use of cumulative haematology files on a laboratory computer.

    abstract::A system is described for the maintenance of cumulative haematology records for selected patients using a laboratory minicomputer with limited storage capacity. Records are indexed by the patient's name and location since, as in the majority of hospital laboratories, there is no unique numbering scheme which covers al...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.30.4.356

    authors: Alexander MK,Corbett MT,Reed RW

    更新日期:1977-04-01 00:00:00

  • The anatomy of the normal placenta.

    abstract::The placenta is the fetal organ providing the interchange between mother and fetus. This organ needs to provide its function such as transport and secretion even during its development and thus all developmental changes need to be in accordance with its function. This review describes development of the placenta durin...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:10.1136/jcp.2008.055277

    authors: Huppertz B

    更新日期:2008-12-01 00:00:00

  • The morphological identification of pathogenic yeasts using carbohydrate media.

    abstract::Eight isolates of C. albicans were used to determine the frequency with which germ tube formation occurred: on rice extract -Tween 80 agar, on its components, and on 1% bactopeptone agar after three hr at 37 degrees C; in 0.5% aqueous solution of various carbohydrates; in various concentrations of glucose; on 0.5 and ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.28.1.18

    authors: Joshi KR,Bremner DA,Parr DN,Gavin JB

    更新日期:1975-01-01 00:00:00

  • Lysozyme expression in microscopic colitis.

    abstract:AIMS:To audit the cellular expression of the innate antibacterial enzyme lysozyme in colonic biopsies from a cohort of patients having microscopic colitis (MC-collagenous colitis (CC) or lymphocytic colitis (LC)). Results were compared with those recorded in patients with inflammatory bowel disease (IBD) of the colon (...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.2010.086850

    authors: Rubio CA

    更新日期:2011-06-01 00:00:00

  • The pattern of involvement of the gastric mucosa in lymphocytic gastritis is predictive of the presence of duodenal pathology.

    abstract:AIM:To determine whether the pattern of involvement of the gastric mucosa in lymphocytic gastritis is predictive of the presence or absence of duodenal pathology. METHODS:50 cases (M:F, 26:24; median age 57 years) diagnosed as lymphocytic gastritis between 1986 and 1998 with concurrent duodenal (D2) biopsies were iden...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.52.11.815

    authors: Hayat M,Arora DS,Wyatt JI,O'Mahony S,Dixon MF

    更新日期:1999-11-01 00:00:00

  • Performance evaluation of the Sysmex haematology XN modular system.

    abstract:BACKGROUND:The Sysmex XN haematology instrument performs automatic reflex testing, depending on sample results. A nucleated red blood cell (NRBC) count is provided on all samples. The instrument has a smaller footprint (34%) than previous Sysmex XE analysers. METHODS:An evaluation comparing all results to the Sysmex X...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jclinpath-2012-200930

    authors: Briggs C,Longair I,Kumar P,Singh D,Machin SJ

    更新日期:2012-11-01 00:00:00

  • Raised concentration of plasma creatine kinase BB isoenzyme in myelodysplasia.

    abstract::A 72 year old woman presented with a suspected myocardial infarction. An echocardiograph showed no acute changes but her plasma creatine kinase (CK) activity was increased at 343 U/l (< 175 normal range). The apparent creatine kinase-MB activity by a CK-M subunit immunoinhibition assay was 350 U/l. In view of the disc...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.47.6.552

    authors: Crook M,Williams A,Sankaralingam A,Tutt P

    更新日期:1994-06-01 00:00:00

  • Laboratory turnround time: closing the loop.

    abstract:AIMS:To institute recommendations from a laboratory turnround time study; to evaluate audit methods; and to quantify improvements achieved. METHODS:Changes to result report distribution and specimen delivery were affected by posting results directly from the laboratory followed by the introduction of a twice daily cou...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.48.4.372

    authors: Smellie WS,Galloway PJ,Johnston JI

    更新日期:1995-04-01 00:00:00

  • Expression of ATM, p53, and the MRE11-Rad50-NBS1 complex in myoepithelial cells from benign and malignant proliferations of the breast.

    abstract:AIMS:To analyse the expression of proteins involved in DNA double strand break detection and repair in the luminal and myoepithelial compartments of benign breast lesions and malignant breast tumours with myoepithelial differentiation. METHODS:Expression of the ataxia telangiectasia (ATM) and p53 proteins was immunohi...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.2004.017434

    authors: Angèle S,Jones C,Reis Filho JS,Fulford LG,Treilleux I,Lakhani SR,Hall J

    更新日期:2004-11-01 00:00:00

  • Immunocytological analysis of the Tn associated antigen 83D4 in serous effusions from patients with cancer: comparison with Tn soluble glycoprotein.

    abstract:AIMS:To determine whether the monoclonal antibody (MoAb) 83D4, previously shown to be highly specific for carcinoma cells, can be used as an immunocytological marker to discriminate between benign and malignant cells in serous effusions; and to test for a correlation between expression of the antigen reacting with MoAb...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.48.5.433

    authors: Beuzelin-Yvraut M,Bourguignat A,Phillips E,Roseto A,Osinaga E

    更新日期:1995-05-01 00:00:00

  • Four cases of human infection with Achromobacter anitratus.

    abstract::Four cases of human infection with Achromobacter anitratus, one of which was fatal, are described. They all occurred at one hospital within a period of two months. The bacteriology, antibiotic sensitivity, and pathogenicity of the organisms are discussed. One of the strains was found to differ from the other three. ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.14.6.670

    authors: ASHLEY DJ,KWANTES W

    更新日期:1961-11-01 00:00:00

  • My approach to pathology of the pituitary gland.

    abstract::The sellar region is the site of a large number of pathological entities arising from the pituitary and adjacent anatomical structures, including brain, blood vessels, nerves and meninges. The surgical pathology of this area requires the accurate identification of neoplastic lesions, including pituitary adenoma and ca...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:10.1136/jcp.2005.031187

    authors: Al-Brahim NY,Asa SL

    更新日期:2006-12-01 00:00:00

  • Quantitation of intraepithelial lymphocytes in human duodenum: what is normal?

    abstract:BACKGROUND:An increase in intraepithelial lymphocytes (IELs) is mandatory for the histological diagnosis of coeliac disease (CD). Currently, duodenal biopsies are used almost exclusively to establish the diagnosis, yet published work continues to cite an upper limit of 40 lymphocytes/100 epithelial cells, a figure deri...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.55.5.393

    authors: Hayat M,Cairns A,Dixon MF,O'Mahony S

    更新日期:2002-05-01 00:00:00

  • PD-L1 expression on routine samples of non-small cell lung cancer: results and critical issues from a 1-year experience of a centralised laboratory.

    abstract:AIMS:Our laboratory is a centralised centre receiving routine non-small cell lung cancer (NSCLC) samples for programmed death ligand-1 (PD-L1) immunohistochemical (IHC) evaluation. Since literature data are not concordant here we review our clinical records to assess the rate of PD-L1 positive and negative NSCLC cases ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jclinpath-2019-205732

    authors: Vigliar E,Malapelle U,Iaccarino A,Acanfora G,Pisapia P,Clery E,De Luca C,Bellevicine C,Troncone G

    更新日期:2019-06-01 00:00:00

  • Myelofibrosis presenting as spinal cord compression.

    abstract::This report describes a case of myelofibrosis presenting as spinal cord compression on account of extramedullary haemopoietic tissue encroaching upon the spinal cord from a large pelvic mass. ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.56.2.154

    authors: Horwood E,Dowson H,Gupta R,Kaczmarski R,Williamson M

    更新日期:2003-02-01 00:00:00

  • New concept in diagnostic endometrial cytology: diagnostic criteria based on composition and architecture of large tissue fragments in smears.

    abstract::Routine endometrial cytology was used instead of curettage as the first step in a morphological investigation of the endometrium in patients with postmenopausal bleeding. Premenopausal women with symptoms or signs indicative of premalignant or malignant disease were also studied using this method. Patients with alarmi...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.39.1.36

    authors: Skaarland E

    更新日期:1986-01-01 00:00:00

  • Evaluation of API 20 STREP system for identifying Listeria species.

    abstract::The API 20 STREP system was used to identify 146 known strains from seven species of the genus Listeria, including both pathogenic and environmental strains. The gallery was easy to use and tests, with the exception of leucine arylamidase (LAP) and starch fermentation (AMD), were simple to interpret. Identification to...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.42.5.548

    authors: MacGowan AP,Marshall RJ,Reeves DS

    更新日期:1989-05-01 00:00:00

  • Use of buffered hypochlorite solution for disinfecting fibrescopes.

    abstract::The possible use of sodium hypochlorite solution buffered to pH 7.6 and containing 100 ppm available chlorine (avCl) for disinfecting fibrescopes was investigated. A flexible fibrescope experimentally contaminated with Pseudomonas putida, Mycobacterium fortuitum, or Bacillus subtilis spores was effectively disinfected...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.35.3.296

    authors: Coates D,Death JE

    更新日期:1982-03-01 00:00:00

  • Comparison of the expression of p53, p21, Bax and the induction of apoptosis between patients with basal cell carcinoma and normal controls in response to ultraviolet irradiation.

    abstract:AIM:Ultraviolet light (UV) is known to cause DNA damage in the epidermis. The damaged DNA is repaired or deleted by apoptosis to prevent the generation of cancer. It has been suggested that a deficient apoptotic mechanism may predispose individuals to skin cancer. Therefore, the response of normal controls and patients...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.55.11.829

    authors: Murphy M,Mabruk MJ,Lenane P,Liew A,McCann P,Buckley A,O Flatharta C,Hevey D,Billet P,Robertson W,Javed S,Leader M,Kay E,Murphy GM

    更新日期:2002-11-01 00:00:00

  • Handwashing and antiseptic-containing soaps in hospital.

    abstract::Two aspects of handwashing in hospital were considered. A study was carried out to examine the contamination of bar soap and containers, and the use of antiseptic soaps in reducing the resident flora of the skin. Swabs were collected from soap dishes on six wards and from a bacteriology laboratory on four consecutive ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.32.7.732

    authors: Jarvis JD,Wynne CD,Enwright L,Williams JD

    更新日期:1979-07-01 00:00:00

  • Comparison of various assays for detection of AFP in differentiating patients with primary hepatocellular carcinoma from controls.

    abstract::Four assays for the detection of alphafetoprotein (AFP) in the diagnosis of primary hepatocellular carcinoma (HCC) were evaluated. The frequency of AFP detection was compared in 89 HCC patients and in 71 patients with chronic active hepatitis/cirrhosis. Of the four assays, immunodiffusion, counterimmunoelectrophoresis...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.33.8.792

    authors: Chan SH,Heng SH,Yo SL,Oon CJ

    更新日期:1980-08-01 00:00:00

  • Simultaneous in situ hybridisation of native mRNA and immunoglobulin detection by conventional immunofluorescence in paraffin wax embedded sections.

    abstract:AIMS:The development of a technique for simultaneous in situ hybridisation for native mRNA and conventional immunofluorescence for cytoplasmic antigens in routine pathology specimens. METHODS:Cocktails of synthetic deoxyoligonucleotides coding for immunoglobulin J chain and kappa light chain were 3' end labelled enzym...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.45.2.114

    authors: Harper SJ,Pringle JH,Gillies A,Allen AC,Layward L,Feehally J,Lauder I

    更新日期:1992-02-01 00:00:00

  • Expression of cytokeratin in the epithelium of dentigerous cysts and odontogenic keratocysts: an aid to diagnosis.

    abstract::Sections of tissue embedded in paraffin wax from 18 selected odontogenic cysts were studied both histologically and immunohistochemically with antibodies to cytokeratins using the indirect peroxidase technique. The cysts were divided on a clinical and histological basis into two equal groups comprising dentigerous cys...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.42.7.736

    authors: MacDonald AW,Fletcher A

    更新日期:1989-07-01 00:00:00

  • An evaluation of the ToxHA test for the detection of antibodies to Toxoplasma gondii in human serum.

    abstract::The titre of antibodies to Toxoplasma gondii was compared in 1985 sera by use of an indirect haemagglutination test (ToxHA test--Wellcome Reagents Ltd) and the dye test. Sera from 42 patients with clinical or serological indications of recent toxoplasmosis were also examined to determine if the ToxHA test would be of ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.33.7.644

    authors: Balfour AH,Bridges JB,Harford JP

    更新日期:1980-07-01 00:00:00

  • Cervical cytology biobanking: quality of DNA from archival cervical Pap-stained smears.

    abstract:AIMS:Cervical cytology biobanking is a feasible concept in cervical pathology and could be an indispensable tool for fundamental and applied molecular biological research. PCR is a powerful molecular technique that can be performed on a variety of cervical sample types including Pap-stained cervical smears. However, si...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.2007.052688

    authors: Boulet GA,Horvath CA,Berghmans S,Moeneclaey LM,Duys IS,Arbyn M,Depuydt CE,Vereecken AJ,Sahebali S,Bogers JJ

    更新日期:2008-05-01 00:00:00

  • A study of the prevalence of vitamin K deficiency in patients with cancer referred to a hospital palliative care team and its association with abnormal haemostasis.

    abstract:BACKGROUND:Many patients with advanced cancer are malnourished. Anorexia is common, as is the use of chemotherapy, which may cause nausea and poor appetite. Ten per cent of these patients experience haemorrhagic events. AIM:Since vitamin K deficiency (VKD) causes bleeding, to establish the prevalence of VKD in patient...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.2007.052498

    authors: Harrington DJ,Western H,Seton-Jones C,Rangarajan S,Beynon T,Shearer MJ

    更新日期:2008-04-01 00:00:00

  • Predictive molecular pathology in the time of COVID-19.

    abstract:AIMS:In the time of COVID-19, predictive molecular pathology laboratories must still timely select oncological patients for targeted treatments. However, the need to respect social distancing measures may delay results generated by laboratory-developed tests based on sequential steps a long hands-on time. Laboratory wo...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jclinpath-2020-206711

    authors: Malapelle U,De Luca C,Iaccarino A,Pepe F,Pisapia P,Russo M,Sgariglia R,Nacchio M,Vigliar E,Bellevicine C,Schmitt FC,Troncone G

    更新日期:2020-05-19 00:00:00

  • Ocular adnexal marginal zone B cell lymphoma infiltrated by IgG4-positive plasma cells.

    abstract:AIMS:To report the clinicopathological characteristics of patients with ocular adnexal marginal zone B cell lymphoma (MZBL) with IgG4-positive plasma cells. METHODS:114 biopsy samples of ocular adnexal MZBLs were analysed. MZBLs with IgG4-positive plasma cells were included when the IgG4:IgG ratio was >40% (IgG4-relat...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1136/jcp.2010.082156

    authors: Kubota T,Moritani S,Yoshino T,Nagai H,Terasaki H

    更新日期:2010-12-01 00:00:00