Abstract:
AIMS:Our laboratory is a centralised centre receiving routine non-small cell lung cancer (NSCLC) samples for programmed death ligand-1 (PD-L1) immunohistochemical (IHC) evaluation. Since literature data are not concordant here we review our clinical records to assess the rate of PD-L1 positive and negative NSCLC cases in real-world practice. METHODS:PD-L1 expression was evaluated by a validated 22C3 IHC laboratory developed test on 211 prospectively collected routine NSCLC samples, received from 10 outside institutions. PD-L1 expression was assessed by the tumour proportion score (TPS) and reported by using a three cut-point system: TPS<1, TPS 1%-49% and TPS≥50%. RESULTS:Overall, 193 out of 211 samples (91.5%) meet the criteria for adequacy (more than 100 viable neoplastic cells). In 62.7% (121/193) of samples TPS was <1%; 17.6% of samples (34/193) expressed PD-L1 with a TPS of 1%-49% and 19.7% (38/193) with a TPS of >50%. There was no significant difference in PD-L1 expression rates between different histotypes and site of sampling. Instead, a statistically significant difference was associated to the type of samples: in fact, cytological samples were more frequently negative for PD-L1 expression (TPS<1%) and less often displayed PD-L1 expression at high levels (TPS>50%) than surgical resections and biopsies. CONCLUSIONS:We present a referral laboratory experience on IHC PD-L1 expression of prospectively collected routine NSCLC samples. Data from the real-world practice can better clarify the percentage of PD-L1 positive and negative cases, to establish benchmarks for good practice standards.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Vigliar E,Malapelle U,Iaccarino A,Acanfora G,Pisapia P,Clery E,De Luca C,Bellevicine C,Troncone Gdoi
10.1136/jclinpath-2019-205732subject
Has Abstractpub_date
2019-06-01 00:00:00pages
412-417issue
6eissn
0021-9746issn
1472-4146pii
jclinpath-2019-205732journal_volume
72pub_type
杂志文章abstract::The ultrastructure of the osteocytes, osteoblasts, osteoclasts, haemopoietic and other connective tissue cells was examined in 27 biopsies from 22 patients with Paget's disease of bone. Electron microscopy showed characteristic nuclear and cytoplasmic inclusions in the osteoclasts of all of the 25 biopsies exhibiting ...
journal_title:Journal of clinical pathology
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journal_title:Journal of clinical pathology
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journal_title:Journal of clinical pathology
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journal_title:Journal of clinical pathology
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journal_title:Journal of clinical pathology
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doi:10.1136/jcp.2008.063156
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journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.2004.017293
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.47.9.862
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.6.523
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journal_title:Journal of clinical pathology
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doi:10.1136/jcp.41.3.289
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journal_title:Journal of clinical pathology
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doi:10.1136/jcp.42.4.371
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journal_title:Journal of clinical pathology
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doi:10.1136/jcp.2004.017137
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journal_title:Journal of clinical pathology
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journal_title:Journal of clinical pathology
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journal_title:Journal of clinical pathology
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