Abstract:
:Increased secretion of matrix metalloproteinases and serine proteinases is well known to be associated with cancer invasion and metastasis. We aimed to elucidate the implication of trypsin, a serine proteinase and a representative digestive enzyme in invasion and metastasis of human carcinomas. Northern blot, RT-PCR and Western blot analyses and immunohistochemical studies were performed to detect and analyze trypsinogen expression in 5 ovarian carcinoma cell lines and 10 human ovarian carcinoma tissues using a DNA probe for trypsinogen I, and monoclonal and polyclonal antibodies to human trypsin I. Among the 5 ovarian carcinoma cell lines, only the MCAS (mucinous cystadenocarcinoma) cell line showed a high level of trypsinogen production and mRNA expression by Western and Northern blot analyses, respectively. However, Southern blot analysis of RT-PCR products could detect considerable levels of trypsinogen mRNA in all ovarian cancer cell lines. In Northern analysis of ovarian cancer tissues, all advanced cancer samples showed trypsinogen gene expression. Serous cystadenocarcinomas exhibited particularly high levels of gene expression. Immunohistochemical staining also detected trypsin in ovarian carcinoma tissues. In contrast, normal ovaries and tumors with low malignant potential did not show trypsinogen expression. Our results demonstrate the extra-pancreatic production and distribution of trypsinogen in human ovarian carcinomas.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Hirahara F,Miyagi Y,Miyagi E,Yasumitsu H,Koshikawa N,Nagashima Y,Kitamura H,Minaguchi H,Umeda M,Miyazaki Kdoi
10.1002/ijc.2910630205subject
Has Abstractpub_date
1995-10-09 00:00:00pages
176-81issue
2eissn
0020-7136issn
1097-0215journal_volume
63pub_type
杂志文章abstract::The expression of two discrete cell properties related to the host natural effector mechanisms, i.e., resistance to damage by H2O2, a cytotoxic product of activated macrophages, and the ability to secrete PGE, which inhibits NK-cell cytotoxicity, has been examined in parental Syrian hamster embryo cells spontaneously ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910440526
更新日期:1989-11-15 00:00:00
abstract::Chemotherapy-induced gastrointestinal toxicity (CIGT) occurs in up to 80% of all patients undergoing cancer treatment, and leads to symptoms such as diarrhoea, abdominal bleeding and pain. There is currently limited understanding of how to predict an individual patient's risk of CIGT. It is believed the gut microbiome...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/ijc.31836
更新日期:2019-05-15 00:00:00
abstract::The purpose was to evaluate alternative cytological screening methods in population-based screening for cervical cancer up to cancer incidence and mortality outcome. Automation-assisted screening was compared to conventional cytological screening in a randomized design. The study was based on follow-up of 503,391 wome...
journal_title:International journal of cancer
pub_type: 杂志文章,随机对照试验
doi:10.1002/ijc.25677
更新日期:2011-03-01 00:00:00
abstract::A glutamine analogue, L-glutamic acid gamma-monohydroxamate (GAH) demonstrated complete cytotoxicity against L1210 cells in culture and marked anti-tumoral activity in vivo against L1210 leukemia and B16 melanoma. In vitro, GAH caused concentration-dependent inhibition of L1210 cell growth, with complete cell death be...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910450428
更新日期:1990-04-15 00:00:00
abstract::Statin use has been suggested to improve prognosis in cancer patients, however, for ovarian cancer, the evidence is sparse. From the Danish Cancer Registry, we identified patients aged 30-84 years with a histologically verified first diagnosis of epithelial ovarian cancer between 2000 and 2013. Data on filled prescrip...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.30738
更新日期:2017-07-15 00:00:00
abstract::Histone deacetylase inhibitors, such as phenylbutyrate, are currently undergoing clinical trials as potential anticancer agents. Phenylbutyrate can induce cell differentiation and apoptosis in a number of cancer cell types and can act in synergy with ionizing radiation and chemotherapy to induce apoptosis. We used the...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10609
更新日期:2002-10-10 00:00:00
abstract::Ether-linked glycerophospholipids (ether lipids, EL) are selectively toxic and anti-proliferative agents against cancer cells in vitro. The reason for such selectivity is not completely clear. Their mechanism of action is mediated through an interaction with the plasma membrane and the membrane lipid composition may m...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910490317
更新日期:1991-09-30 00:00:00
abstract::The relationship between breast cancer-associated fatty acid synthase (FAS; oncogenic antigen-519) and chemotherapy-induced cell damage has not been studied. We examined the ability of C75, a synthetic slow-binding inhibitor of FAS activity, to modulate the cytotoxic activity of the microtubule-interfering agent Taxol...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.20754
更新日期:2005-05-20 00:00:00
abstract::Fluoropyrimidines induce cytotoxicity, in part, by inhibiting the proliferation-coordinated enzyme thymidylate synthase (TS), which is essential for DNA synthesis. Tumor TS levels are clinically predictive of post-surgical tumor recurrence and of response to fluoropyrimidine chemotherapy. Fluoropyrimidine drug toxicit...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:
更新日期:2000-11-01 00:00:00
abstract::There is a well-established link between inflammation and cancer of various organs, but little data are available on inflammation-associated markers of diagnostic and prognostic clinical utility in pulmonary malignancy. Blood samples were prospectively collected from 75 resectable lung cancer patients before surgery a...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29822
更新日期:2016-02-15 00:00:00
abstract::The colo-rectum and stomach are the 2 sites for which the role of diet in the etiology of cancer is most heavily implicated. Quantitative estimates as to the proportion of cancer deaths attributable to diet vary considerably. This may be partially due to the application of the concept of population attributable risk w...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910400509
更新日期:1987-11-15 00:00:00
abstract::Exfoliated nasopharyngeal (NP) cells from 62 normal Cantonese Chinese having IgA/VCA antibodies for more than a year and from 39 similar persons without IgA/VCA antibodies, were tested for the presence of EBV/DNA sequences by spot followed by blot hybridization tests, using the cloned internal repeat of B95-8 viral DN...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910320504
更新日期:1983-11-15 00:00:00
abstract::China was one of the countries with the highest esophagus cancer risk in the world during the 1970s. This report provides data on time trends of esophagus cancer incidence and mortality during the 1970s-90s in selected geographic areas of China. Information on newly diagnosed cancer cases and cancer deaths is based on...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10706
更新日期:2002-11-20 00:00:00
abstract::The recently described PGM-2 leukemia displays a hierarchical structure with bipotential stem cells, B-lymphocyte and macrophage progenitor cells, and post-mitotic end cells. Because the different cell types can easily be identified in vitro by clonal culture assays and simple staining procedures, this leukemia is a u...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910600515
更新日期:1995-03-03 00:00:00
abstract::Secreted frizzled-related proteins (SFRPs) are antagonists of the Wnt signaling pathway whose epigenetic downregulation have been shown to be involved in hepatocarcinogenesis. However, dysregulation of SFRPs induced by hepatitis B virus (HBV) X protein (HBx) has never been studied in HBV-related hepatocellular carcino...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28697
更新日期:2014-08-01 00:00:00
abstract::When effector cells from most individuals are tested directly against a number of target cells for cell-mediated cytotoxicity, most of the reactivity is non-selective. This apparent non-selectiveness is a result of specific activity against multiple antigens on target cells by each effector suspension. The specific re...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910190603
更新日期:1977-06-15 00:00:00
abstract::An experiment in which rats were injected intrapleurally with asbestos, the injection taking place at an age of either 2 or 10 months, is reported. In the former group 19 out of 48 rats developed a mesothelioma compared with 17 out of 90 in the latter group. However, by considering the two groups at equal times after ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910170410
更新日期:1976-04-15 00:00:00
abstract::MART-1/MelanA and Pmel17/gp100 are melanoma-associated antigens (MAAs) that can be recognized by tumor-infiltrating lymphocytes (TILs) capable of mediating successful adoptive therapy in vivo. Analysis of melanoma cell lines in vitro has demonstrated that heterogeneous antigen expression in the context of class I MHC ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19980209)75:4<517::aid-ijc
更新日期:1998-02-09 00:00:00
abstract::Our aim was to determine the long-term outcome and the possible role of neoadjuvant (preoperative) radiation therapy for breast cancers unsuitable for primary conservative surgery. From 1977 to 1992, 75 unifocal non-inflammatory and non-metastatic T2 and T3 breast cancers were treated in our department. All these pati...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.1024
更新日期:2001-08-20 00:00:00
abstract::To better understand the role of individual and lifestyle factors in human disease, an exposome-wide association study was performed to investigate within a single-study anthropometry measures and lifestyle factors previously associated with B-cell lymphoma (BCL). Within the European Prospective Investigation into Can...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.33369
更新日期:2020-10-31 00:00:00
abstract::The transcription factor grainyhead-like 2 (GRHL2) plays a crucial role in various developmental processes. Although GRHL2 recently has attracted considerable interest in that it could be identified as a novel suppressor of the epithelial-to-mesenchymal transition, evidence is emerging that GRHL2 also exhibits tumour-...
journal_title:International journal of cancer
pub_type: 杂志文章,meta分析
doi:10.1002/ijc.29841
更新日期:2016-02-15 00:00:00
abstract::Responses to the genotoxic effect of bleomycin in lymphocytes of blood cultures, expressed as the average number of chromatid breaks per cell (b/c), varied from less than 0.20 to more than 2.00 in 335 normal individuals. More than 11% of the subjects tested showed a b/c rate above 1.00 and more than 22% showed a b/c r...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910430310
更新日期:1989-03-15 00:00:00
abstract::Oral squamous cell carcinoma (OSCC) is the most common malignancy representing 90% of all forms of oral cancer worldwide. Although great efforts have been made in the past decades, the 5-year survival rate of OSCC patients is no more than 60% due to tumor metastasis and subsequent recurrence. The metastasis from the p...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/ijc.33352
更新日期:2020-10-22 00:00:00
abstract::In our study, we aimed to investigate the expression of N-cadherin and E-cadherin and their dependency on epithelial-mesenchymal transition regulators SNAI1, SIP1 and TWIST in human colon cancer. Expression of E-cadherin and N-cadherin was examined by immunohistochemistry in 80 colon carcinomas by using paraffin embed...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.20317
更新日期:2004-09-20 00:00:00
abstract::NK cell recognition of tumor cells is mediated by a delicate balance of signals received by MHC class I-binding inhibitory NK cell receptors and activating NK cell receptors, which mainly bind to virus-, stress- or tumor-induced ligands. In addition, adhesion molecules such as the intercellular adhesion molecule-1 (IC...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25442
更新日期:2011-03-01 00:00:00
abstract::The major obstacle preventing effective treatment of melanoma is the biological heterogeneity of tumor cells. This study was performed to determine clonal genetic heterogeneity within primary melanoma and the evolution of these heterogeneous sub-clones during disease progression. DNA samples were obtained from 44 morp...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:
更新日期:2000-02-15 00:00:00
abstract::TP53 is mutated in 20-25% of aggressive B-cell lymphoma (B-NHL). To date, no studies have addressed the impact of TP53 mutations in prospective clinical trial cohorts. To evaluate the impact of TP53 mutation to current risk models in aggressive B-NHL, we investigated TP53 gene mutations within the RICOVER-60 trial. Of...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1002/ijc.30838
更新日期:2017-10-01 00:00:00
abstract::Complement-dependent cytotoxicity against melanoma cells was demonstrated with a microassay in sera from melanoma patients. The response was tumor-specific and histologic type-specific since 16 out of 52 (30%) melanoma patient sera taken before surgery reacted against melanoma cells, whereas 3 out of 43 (7%) control s...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910160504
更新日期:1975-11-15 00:00:00
abstract::Spreading is a critical process involved in motility and growth of tumor cells during the metastatic cascade. Focal adhesion kinase, src-proteins and PKC have been reported to participate in the regulation of cytoskeleton organization in both normal and transformed cells during spreading. The role of other signaling e...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19970529)71:5<881::aid-ijc
更新日期:1997-05-29 00:00:00
abstract::It has been demonstrated that the chemopreventive agent N-(4-hydroxyphenyl)retinamide (4-HPR) induces apoptotic cell death, but recent data has suggested that late stage/recurrent tumours lose their response to 4-HPR-induced cell death by mechanisms that are unknown. Our study investigated the ability of 4-HPR to indu...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.22263
更新日期:2006-12-15 00:00:00