Human and rat macrophages mediate fungistatic activity against Rhizopus species differently: in vitro and ex vivo studies.

Abstract:

:Both rat alveolar macrophages and a human macrophages cell line with characteristics of human tissue (e.g., alveolar) macrophages (THP-1) were found to inhibit the germination of Rhizopus spores. However, the conditions under which fungistatic activity occurs are different for these two cell types. The inhibition of Rhizopus spore germination by rat alveolar macrophages requires the activation of macrophages and the presence of serum and L-arginine. During rat alveolar macrophage-mediated fungistatic activity, L-arginine is oxidized to nitric. Human macrophage-mediated fungistatic activity is similar to that mediated by rat macrophages in terms of the serum requirement, but it does not require L-arginine. Human macrophages did not produce any nitrite detectable by the colorimetric assay. Their ability to inhibit germination was enhanced by the combination of endotoxin and gamma interferon. The inhibition of Rhizopus spore germination by rat alveolar macrophages is thus mediated by the generation of nitric oxide, whereas the mechanism of similar inhibition by human macrophages remains poorly understood. Serum samples from diabetic rats as well as from patients with diabetes or uremia decreased the inhibitory effect of macrophages on spore germination. Dialysis of the serum samples against a buffered salt solution antagonized this phenomenon, indicating that a low-molecular-weight factor in the sera of patients with diabetes or uremia may modulate local antifungal defense mechanisms. The absence of L-arginine-dependent nitrogen oxidation in human macrophages, compared with its presence in rat alveolar macrophages, under conditions during which fungistatic activity occurs suggests that this phenomenon is species specific.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Jorens PG,Boelaert JR,Halloy V,Zamora R,Schneider YJ,Herman AG

doi

10.1128/IAI.63.11.4489-4494.1995

subject

Has Abstract

pub_date

1995-11-01 00:00:00

pages

4489-94

issue

11

eissn

0019-9567

issn

1098-5522

journal_volume

63

pub_type

杂志文章
  • Protection against colibacillosis in neonatal piglets by immunization of dams with procholeragenoid.

    abstract::Protection against colibacillosis in neonatal piglets was obtained by immunization of pregnant dams with procholeragenoid. Procholeragenoid is a stable high-molecular-weight aggregate of cholera toxin formed during the heating of cholera toxin. Procholeragenoid retained approximately 1% of the toxicity of native toxin...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.35.3.887-894.1982

    authors: Fürer E,Cryz SJ Jr,Dorner F,Nicolet J,Wanner M,Germanier R

    更新日期:1982-03-01 00:00:00

  • Macrophage activation during experimental murine brucellosis: a basis for chronic infection.

    abstract::Evidence is presented that the chronicity of infection in CBA mice after injection of Brucella abortus 19 is related to a number of factors: (i) the relative resistance of B. abortus to macrophage killing, which allowed some bacteria to survive the peak of macrophage activity occurring at 14 days; (ii) the decline in ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.23.2.197-205.1979

    authors: Cheers C,Pagram F

    更新日期:1979-02-01 00:00:00

  • Complement activation by whole endotoxin is blocked by a monoclonal antibody to factor B.

    abstract::Sepsis is both a common and, despite present-day therapy, a serious disease. The pathophysiology of the septic response is a complex, multifactorial phenomenon which in part involves the activation of complement by bacterial endotoxin. A monoclonal antibody to human complement factor B, code-named 1H5, which was capab...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.62.10.4549-4555.1994

    authors: Clardy CW

    更新日期:1994-10-01 00:00:00

  • Chlamydia trachomatis infection of human mesothelial cells alters proinflammatory, procoagulant, and fibrinolytic responses.

    abstract::In this study we demonstrate the capability of Chlamydia trachomatis to infect cultured human mesothelial cell (MC) monolayers and to induce the production of the proinflammatory cytokines interleukin 1beta (IL-1beta) and IL-8. Seventy-two hours after initial infection, both the procoagulant activity of MC and the act...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.66.5.2352-2355.1998

    authors: van Westreenen M,Pronk A,Diepersloot RJ,de Groot PG,Leguit P

    更新日期:1998-05-01 00:00:00

  • Antibody response to Epstein-Barr virus in infectious mononucleosis.

    abstract::Altogether 171 serum specimens from 58 patients with heterophil antibody-positive infectious monomucleosis were studied for antibody response to Epstein-Barr virus (EBV). The sera were tested for fluorescent immunoglobulin G (IgG) and IgM gel-precipitating (GP) and complement-fixing (CF) antibodies to EBV. All 58 pati...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.11.1.42-51.1975

    authors: Nikoskelainen J,Hänninen P

    更新日期:1975-01-01 00:00:00

  • Protective capacity of antibodies against Escherichia coli and K antigens.

    abstract::Antibodies to Escherichia coli O and K antigens were raised in rabbits by repeated immunizations with whole, Formalin-killed and, later, liver bacteria. The serum antibody levels were determined with the ammonium sulfate precipitation technique after radioiodinating the antigens. The K antigens had to be conjugated to...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.17.2.286-289.1977

    authors: Kaijser B,Ahlstedt S

    更新日期:1977-08-01 00:00:00

  • Serotype diversity and antigen variation among invasive isolates of Ureaplasma urealyticum from neonates.

    abstract::Ureaplasma urealyticum has previously been isolated from the cultured cerebrospinal fluid of 13 of 418 newborn infants; additional bloodstream isolates were obtained from the same population. Ten of the 13 cerebrospinal fluid and 3 bloodstream isolates were available for serotyping in the present study. By the use of ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.60.8.3472-3474.1992

    authors: Zheng X,Watson HL,Waites KB,Cassell GH

    更新日期:1992-08-01 00:00:00

  • Induction of proinflammatory cytokines from human respiratory epithelial cells after stimulation by nontypeable Haemophilus influenzae.

    abstract::Nontypeable Haemophilus influenzae (NTHi) causes repeated respiratory infections in patients with chronic lung diseases. These infections are characterized by a brisk inflammatory response which results in the accumulation of polymorphonucleated cells in the lungs and is dependent on the expression and secretion of pr...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.68.8.4430-4440.2000

    authors: Clemans DL,Bauer RJ,Hanson JA,Hobbs MV,St Geme JW 3rd,Marrs CF,Gilsdorf JR

    更新日期:2000-08-01 00:00:00

  • Phosphorylation events in the multiple gene regulator of group A Streptococcus significantly influence global gene expression and virulence.

    abstract::Whole-genome sequencing analysis of ∼800 strains of group A Streptococcus (GAS) found that the gene encoding the multiple virulence gene regulator of GAS (mga) is highly polymorphic in serotype M59 strains but not in strains of other serotypes. To help understand the molecular mechanism of gene regulation by Mga and i...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.03023-14

    authors: Sanson M,Makthal N,Gavagan M,Cantu C,Olsen RJ,Musser JM,Kumaraswami M

    更新日期:2015-06-01 00:00:00

  • GapA and CrmA coexpression is essential for Mycoplasma gallisepticum cytadherence and virulence.

    abstract::It was previously demonstrated that avirulent Mycoplasma gallisepticum strain R(high) (passage 164) is lacking three proteins that are expressed in its virulent progenitor, strain R(low) (passage 15). These proteins were identified as the cytadhesin molecule GapA, the putative cytadhesin-related molecule CrmA, and a c...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.70.12.6839-6845.2002

    authors: Papazisi L,Frasca S Jr,Gladd M,Liao X,Yogev D,Geary SJ

    更新日期:2002-12-01 00:00:00

  • PsrA is a positive transcriptional regulator of the type III secretion system in Pseudomonas aeruginosa.

    abstract::The type III secretion system (TTSS) of Pseudomonas aeruginosa is induced in vivo upon contact with eukaryotic cells and in vitro by calcium depletion in culture medium. We have observed a previously identified protein, PsrA, necessary for full activation of TTSS gene expression in P. aeruginosa. Electrophoretic mobil...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.74.2.1121-1129.2006

    authors: Shen DK,Filopon D,Kuhn L,Polack B,Toussaint B

    更新日期:2006-02-01 00:00:00

  • Identification of a surrogate marker for infection in the African green monkey model of inhalation anthrax.

    abstract::In 2001, a bioterrorism attack involving Bacillus anthracis spore-laced letters resulted in 22 cases of inhalation anthrax, with five fatalities. This incident identified gaps in our health care system and precipitated a renewed interest in identifying both therapeutics and rapid diagnostic assays. To address those ga...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00520-08

    authors: Rossi CA,Ulrich M,Norris S,Reed DS,Pitt LM,Leffel EK

    更新日期:2008-12-01 00:00:00

  • Divergent role of gamma interferon in a murine model of pulmonary versus systemic Klebsiella pneumoniae infection.

    abstract::Klebsiella pneumoniae is a leading cause of both community-acquired and nosocomial gram-negative-bacterial pneumonia. A further clinical complication of pulmonary K. pneumoniae infection is dissemination of bacteria from the lung into the peripheral blood, resulting in bacteremia concurrent with the localized pulmonar...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.70.11.6310-6318.2002

    authors: Moore TA,Perry ML,Getsoian AG,Newstead MW,Standiford TJ

    更新日期:2002-11-01 00:00:00

  • Humoral immune response in acquired immunity to chlamydial genital infection of female guinea pigs.

    abstract::Immunity to reinfection in the genital tract of female guinea pigs with the agent of guinea pig inclusion conjunctivitis was found to be dependent upon an intact humoral immune response. Cell-mediated immunity in the absence of humoral immunity had no apparent role in resistance to challenge infection. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.39.1.463-465.1983

    authors: Rank RG,Barron AL

    更新日期:1983-01-01 00:00:00

  • Detection of Mycobacterium tuberculosis group organisms in human and mouse joint tissue by reverse transcriptase PCR: prevalence in diseased synovial tissue suggests lack of specific association with rheumatoid arthritis.

    abstract::Infection with mycobacterial species, including Mycobacterium tuberculosis, has long been implicated in the etiopathology of rheumatoid arthritis (RA) on the basis of clinical and pathological similarities between tuberculosis and RA. Despite evidence of immune responses to mycobacterial antigens in RA patient synovia...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.69.3.1821-1831.2001

    authors: Kempsell KE,Cox CJ,McColm AA,Bagshaw JA,Reece R,Veale DJ,Emery P,Isaacs JD,Gaston JS,Crowe JS

    更新日期:2001-03-01 00:00:00

  • Lipopolysaccharide structure determines ionic and hydrophobic binding of a cationic antimicrobial neutrophil granule protein.

    abstract::Bactericidal activity and binding of a 57,000-dalton cationic antimicrobial neutrophil granule protein (CAP57) are determined by the presence on bacteria of O-antigen polysaccharide chains and the availability of negatively charged groups in the lipid A region, the inner core region, or both regions of lipopolysacchar...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.56.6.1589-1592.1988

    authors: Farley MM,Shafer WM,Spitznagel JK

    更新日期:1988-06-01 00:00:00

  • Resistance of calves to Cryptosporidium parvum: effects of age and previous exposure.

    abstract::Cryptosporidium parvum is a coccidian parasite that causes diarrheal disease in many vertebrate species, including young (less than or equal to 1 month old) calves. Older calves and adult cattle are resistant to infection. In this study, newborn calves were raised in isolation from C. parvum for 1 week to 3 months bef...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.58.7.2237-2240.1990

    authors: Harp JA,Woodmansee DB,Moon HW

    更新日期:1990-07-01 00:00:00

  • PspC, a pneumococcal surface protein, binds human factor H.

    abstract::PspC was found to bind human complement factor H (FH) by Western blot analysis of D39 (pspC(+)) and an isogenic mutant TRE108 (pspC). We confirmed that PspA does not bind FH, while purified PspC binds FH very strongly. The binding of FH to exponentially growing pneumococci varied among different isolates when analyzed...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.69.5.3435-3437.2001

    authors: Dave S,Brooks-Walter A,Pangburn MK,McDaniel LS

    更新日期:2001-05-01 00:00:00

  • Detection of antibody-accessible proteins on the cell surface of Haemophilus influenzae type b.

    abstract::A radioimmunoprecipitation method has been devised which permits the direct identification of those proteins which are both exposed on the cell surface of Haemophilus influenzae type b and accessible to antibody. Both extrinsically and intrinsically radiolabeled cells of this organism were employed as the sources of a...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.33.3.950-953.1981

    authors: Hansen EJ,Frisch CF,Johnston KH

    更新日期:1981-09-01 00:00:00

  • Toll-like receptor 6 plays an important role in host innate resistance to Brucella abortus infection in mice.

    abstract::Brucella abortus is recognized by several Toll-like receptor (TLR)-associated pathways triggering proinflammatory responses that affect both the nature and intensity of the immune response. Previously, we demonstrated that B. abortus-mediated dendritic cell (DC) maturation and control of infection are dependent on the...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01356-12

    authors: de Almeida LA,Macedo GC,Marinho FA,Gomes MT,Corsetti PP,Silva AM,Cassataro J,Giambartolomei GH,Oliveira SC

    更新日期:2013-05-01 00:00:00

  • Effect of exogenous nucleotides on Ca2+ dependence and V antigen synthesis in Yersinia pestis.

    abstract::Cells of Yersinia pestis strain EV76 are known to cease growth after a shift from 26 to 37 degrees C in neutral Ca2+-deficient medium; this effect is potentiated by Mg2+. With 2.5 mM Mg2+ and no added Ca2+, restriction was relaxed at elevated pH at which maximum cell yields occurred in the presence of 20 mM exogenous ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.38.3.953-959.1982

    authors: Zahorchak RJ,Brubaker RR

    更新日期:1982-12-01 00:00:00

  • Protection against Mycobacterium tuberculosis infection by CD8+ T cells requires the production of gamma interferon.

    abstract::The role of CD8 T cells in controlling Mycobacterium tuberculosis infections in mice was confirmed by comparing the levels of growth of the organism in control, major histocompatibility complex class II knockout, and athymic mice and by transferring T-cell populations into athymic mice. By using donor mice which were ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.66.2.830-834.1998

    authors: Tascon RE,Stavropoulos E,Lukacs KV,Colston MJ

    更新日期:1998-02-01 00:00:00

  • Potential for free radical-induced lipid peroxidation as a cause of endothelial cell injury in Rocky Mountain spotted fever.

    abstract::Cells infected by Rickettsia rickettsii, the causative agent of Rocky Mountain spotted fever, display unusual intracellular morphological changes characterized by dilatation of the membranes of the endoplasmic reticulum and outer nuclear envelope. These changes are consistent with those that might be expected to occur...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.56.12.3110-3115.1988

    authors: Silverman DJ,Santucci LA

    更新日期:1988-12-01 00:00:00

  • Hemagglutination activity and colonization factor antigens I and II in enterotoxigenic and non-enterotoxigenic strains of Escherichia coli isolated from humans.

    abstract::We examined 205 enterotoxigenic strains of Escherichia coli for colonization factor antigens (CFA) I and II, using an immunodiffusion technique with specific antisera. A total of 36 strains of serogroups O63, O78, O114, O128, and O153 and 1 rough strain possessed CFA/I and gave a single precipitin line; 47 strains of ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.36.1.189-197.1982

    authors: Cravioto A,Scotland SM,Rowe B

    更新日期:1982-04-01 00:00:00

  • Immunochemistry of the streptococcal group R cell wall polysaccharide antigen.

    abstract::The group R streptococcal group antigen has been shown to be a polysaccharide located at the surface of the cell wall of the organism. The antigen was extracted from cell walls in 0.05 n HCl or 5% trichloracetic acid at 100 C, from whole cells at room temperature in 0.85% NaCl or 0.1 m acetate (pH 5.0), and by sonic o...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.5.1.91-97.1972

    authors: Soprey P,Slade HD

    更新日期:1972-01-01 00:00:00

  • Assessment of protease (elastase) as a Pseudomonas aeruginosa virulence factor in experimental mouse burn infection.

    abstract::The data presented indicate that in experimental Pseudomonas aeruginosa infection of mice, protease enhances the virulence of the organism. Anesthetized CBA/Lü mice were subjected to a 15-s flame burn and infected with a wild-type protease-producing strain and two of its protease-deficient mutants. The average bacteri...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.24.1.181-187.1979

    authors: Pavlovskis OR,Wretlind B

    更新日期:1979-04-01 00:00:00

  • Targeted disruption of Toxoplasma gondii serine protease inhibitor 1 increases bradyzoite cyst formation in vitro and parasite tissue burden in mice.

    abstract::As an intracellular protozoan parasite, Toxoplasma gondii is likely to exploit proteases for host cell invasion, acquisition of nutrients, avoidance of host protective responses, escape from the parasitophorous vacuole, differentiation, and other activities. T. gondii serine protease inhibitor 1 (TgPI1) is the most ab...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.06167-11

    authors: Pszenny V,Davis PH,Zhou XW,Hunter CA,Carruthers VB,Roos DS

    更新日期:2012-03-01 00:00:00

  • MyD88, but not toll-like receptors 4 and 2, is required for efficient clearance of Brucella abortus.

    abstract::It is not clear how the host initially recognizes and responds to infection by gram-negative pathogenic Brucella spp. It was previously shown (D. S. Weiss, B. Raupach, K. Takeda, S. Akira, and A. Zychlinsky, J. Immunol. 172:4463-4469, 2004) that the early macrophage response against gram-negative bacteria is mediated ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.73.8.5137-5143.2005

    authors: Weiss DS,Takeda K,Akira S,Zychlinsky A,Moreno E

    更新日期:2005-08-01 00:00:00

  • Campylobacter-Wolinella group organisms are the only oral bacteria that form arylsulfatase-active colonies on a synthetic indicator medium.

    abstract::Most oral bacteria tested formed colonies on a chemically defined medium with a chromogenic arylsulfatase substrate. Arylsulfatase activity was, however, restricted to Campylobacter-Wolinella group organisms, including Wolinella recta, a possible periodontopathogen. W. recta was the only arylsulfatase-active species a...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.57.5.1380-1383.1989

    authors: Wyss C

    更新日期:1989-05-01 00:00:00

  • Functional Properties of Helicobacter pylori VacA Toxin m1 and m2 Variants.

    abstract::Helicobacter pylori colonizes the gastric mucosa and secretes a pore-forming toxin (VacA). Two main types of VacA, m1 and m2, can be distinguished by phylogenetic analysis. Type m1 forms of VacA have been extensively studied, but there has been relatively little study of m2 forms. In this study, we generated H. pylori...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00032-20

    authors: Caston RR,Sierra JC,Foegeding NJ,Truelock MD,Campbell AM,Frick-Cheng AE,Bimczok D,Wilson KT,McClain MS,Cover TL

    更新日期:2020-05-20 00:00:00