当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Evidence for insulin-induced internalization and degradation of insulin receptors in rat adipocytes.

Evidence for insulin-induced internalization and degradation of insulin receptors in rat adipocytes.

Abstract：

:We have investigated the theory that the insulin-induced loss of insulin binding from adipocytes is due to internalization of insulin receptors. Cell-surface receptors were assessed by the binding capacity of intact cells at 16 degrees C. Total (i.e., cell-surface plus intracellular) receptors were assessed by solubilizing the cells in 1% Triton X-100 and then measuring binding by the solubilized extract. Intracellular receptors were measured by treating the cells with trypsin before solubilizing them. The trypsin treatment removed greater than 90% of the cell-surface binding, so that any significant binding by soluble extracts of these cells must represent intracellular receptors. Adipocytes were incubated with insulin (100 ng/ml) with or without chloroquine (0.2 mM) for 4 hr. Insulin alone resulted in a 62% loss of cell-surface receptors, but only a 46% loss of total receptors, and a 170% increase in intracellular receptors, suggesting that the lost cell-surface receptors were internalized, where some were degraded. Insulin in the presence of chloroquine resulted in a 34% loss of cell-surface receptors, but no loss of total receptors, and a 300% increase in intracellular receptors. Thus, in the presence of chloroquine receptors were internalized but not degraded. The loss of cell-surface receptors and appearance of intracellular receptors were time and dose dependent and were linearly related. These results demonstrate that insulin causes translocation of insulin receptors from the cell surface to the cell interior, where they can be degraded (or inactivated) by a chloroquine-sensitive process.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Green A,Olefsky JM

doi

10.1073/pnas.79.2.427

subject

Has Abstract

pub_date

1982-01-01 00:00:00

pages

427-31

issue

2

eissn

0027-8424

issn

1091-6490

journal_volume

79

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Physical interaction between the serotonin transporter and neuronal nitric oxide synthase underlies reciprocal modulation of their activity.

    abstract：:The spatiotemporal regulation of neurotransmitter transporters involves proteins that interact with their intracellular domains. Using a proteomic approach, we identified several proteins that interact with the C terminus of the serotonin transporter (SERT). These included neuronal nitric oxide synthase (nNOS), a PSD-...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0610964104

    authors： Chanrion B,Mannoury la Cour C,Bertaso F,Lerner-Natoli M,Freissmuth M,Millan MJ,Bockaert J,Marin P

    更新日期：2007-05-08 00:00:00

  • Tungsten isotopic constraints on the age and origin of chondrules.

    abstract：:Chondrules may have played a critical role in the earliest stages of planet formation by mediating the accumulation of dust into planetesimals. However, the origin of chondrules and their significance for planetesimal accretion remain enigmatic. Here, we show that chondrules and matrix in the carbonaceous chondrite Al...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1524980113

    authors： Budde G,Kleine T,Kruijer TS,Burkhardt C,Metzler K

    更新日期：2016-03-15 00:00:00

  • Circular dichroism and magnetic circular dichroism of nitrogenase proteins.

    abstract：:Circular dichroism (CD) and magnetic circular dichroism (MCD) spectra of nitrogenase components (MoFe protein and Fe protein) from Azotobacter vinelandii (Av) and Klebsiella pneumoniae (Kp) have been obtained in the near infrared-visible-near ultraviolet spectral region. Previously, visible CD was reported to be absen...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.76.6.2585

    authors： Stephens PJ,McKenna CE,Smith BE,Nguyen HT,McKenna MC,Thomson AJ,Devlin F,Jones JB

    更新日期：1979-06-01 00:00:00

  • Transcriptional profiling of endogenous germ layer precursor cells identifies dusp4 as an essential gene in zebrafish endoderm specification.

    abstract：:A major goal for developmental biologists is to define the behaviors and molecular contents of differentiating cells. We have devised a strategy for isolating cells from diverse embryonic regions and stages in the zebrafish, using computer-guided laser photoconversion of injected Kaede protein and flow cytometry. This...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0805589105

    authors： Brown JL,Snir M,Noushmehr H,Kirby M,Hong SK,Elkahloun AG,Feldman B

    更新日期：2008-08-26 00:00:00

  • Energy transducing redox steps of the Na+-pumping NADH:quinone oxidoreductase from Vibrio cholerae.

    abstract：:Na(+)-NQR is a unique respiratory enzyme that couples the free energy of electron transfer reactions to electrogenic pumping of sodium across the cell membrane. This enzyme is found in many marine and pathogenic bacteria where it plays an analogous role to the H(+)-pumping complex I. It has generally been assumed that...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1002866107

    authors： Juárez O,Morgan JE,Nilges MJ,Barquera B

    更新日期：2010-07-13 00:00:00

  • Hijacking a hydroxyethyl unit from a central metabolic ketose into a nonribosomal peptide assembly line.

    abstract：:Nonribosomal peptide synthetases (NRPSs) usually catalyze the biosynthesis of peptide natural products by sequential selection, activation, and condensation of amino acid precursors. It was reported that some fatty acids, α-ketoacids, and α-hydroxyacids originating from amino acid metabolism as well as polyketide-deri...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1204232109

    authors： Peng C,Pu JY,Song LQ,Jian XH,Tang MC,Tang GL

    更新日期：2012-05-29 00:00:00

  • Saposin A: second cerebrosidase activator protein.

    abstract：:Saposin A, a heat-stable 16-kDa glycoprotein, was isolated from Gaucher disease spleen and purified to homogeneity. Chemical sequencing from its amino terminus and of peptides obtained by digestion with protease from Staphylococcus aureus strain V-8 demonstrated that saposin A is derived from proteolytic processing of...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.86.9.3389

    authors： Morimoto S,Martin BM,Yamamoto Y,Kretz KA,O'Brien JS,Kishimoto Y

    更新日期：1989-05-01 00:00:00

  • Fluorescence-activated sorting of totipotent embryonic stem cells expressing developmentally regulated lacZ fusion genes.

    abstract：:Murine embryonic stem (ES) cells were infected with a retrovirus promoter trap vector, and clones expressing lacZ fusion genes (LacZ+) were isolated by fluorescence-activated cell sorting (FACS). Of 12 fusion genes tested, 1 was repressed when ES cells were allowed to differentiate in vitro. Two of three lacZ fusion g...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.15.6721

    authors： Reddy S,Rayburn H,von Melchner H,Ruley HE

    更新日期：1992-08-01 00:00:00

  • Human-started wildfires expand the fire niche across the United States.

    abstract：:The economic and ecological costs of wildfire in the United States have risen substantially in recent decades. Although climate change has likely enabled a portion of the increase in wildfire activity, the direct role of people in increasing wildfire activity has been largely overlooked. We evaluate over 1.5 million g...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1617394114

    authors： Balch JK,Bradley BA,Abatzoglou JT,Nagy RC,Fusco EJ,Mahood AL

    更新日期：2017-03-14 00:00:00

  • Inflammation and mitochondrial fatty acid beta-oxidation link obesity to early tumor promotion.

    abstract：:Obesity is associated with increased risk for developing pancreatic cancer, and it is suggested that insulin resistance provides the missing link. Here we demonstrate that under the context of genetic susceptibility, a high fat diet (HFD) predisposes mice with oncogenic K-ras activation to accelerated pancreatic intra...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0802864106

    authors： Khasawneh J,Schulz MD,Walch A,Rozman J,Hrabe de Angelis M,Klingenspor M,Buck A,Schwaiger M,Saur D,Schmid RM,Klöppel G,Sipos B,Greten FR,Arkan MC

    更新日期：2009-03-03 00:00:00

  • The reaction of N-iodosuccinimide with the goitrogens: a model for the active iodine of the thyroid gland.

    abstract：:The reactivities of N-iodosuccinimide and iodine with various goitrogenic compounds were studied with dimethylsulfoxide through which nitrogen had been bubbled as a solvent. Although iodine does not react with all of the compounds, N-iodosuccinimide does react with all of them. N-Iodosuccinimide may, therefore, serve ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.70.5.1401

    authors： Jirousek L,Soodak M

    更新日期：1973-05-01 00:00:00

  • Suppression of tumorigenicity by the wild-type tuberous sclerosis 2 (Tsc2) gene and its C-terminal region.

    abstract：:The Tsc2 gene, which is mutationally inactivated in the germ line of some families with tuberous sclerosis, encodes a large, membrane-associated GTPase activating protein (GAP) designated tuberin. Studies of the Eker rat model of hereditary cancer strongly support the role of Tsc2 as a tumor suppressor gene. In this s...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.17.9154

    authors： Jin F,Wienecke R,Xiao GH,Maize JC Jr,DeClue JE,Yeung RS

    更新日期：1996-08-20 00:00:00

  • Quantitative phosphoproteomic analysis reveals vasopressin V2-receptor-dependent signaling pathways in renal collecting duct cells.

    abstract：:Vasopressin's action in renal cells to regulate water transport depends on protein phosphorylation. Here we used mass spectrometry-based quantitative phosphoproteomics to identify signaling pathways involved in the short-term V2-receptor-mediated response in cultured collecting duct cells (mpkCCD) from mouse. Using St...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0910646107

    authors： Rinschen MM,Yu MJ,Wang G,Boja ES,Hoffert JD,Pisitkun T,Knepper MA

    更新日期：2010-02-23 00:00:00

  • Structure of the rare archaeal biosphere and seasonal dynamics of active ecotypes in surface coastal waters.

    abstract：:Marine Archaea are important players among microbial plankton and significantly contribute to biogeochemical cycles, but details regarding their community structure and long-term seasonal activity and dynamics remain largely unexplored. In this study, we monitored the interannual archaeal community composition of abun...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1216863110

    authors： Hugoni M,Taib N,Debroas D,Domaizon I,Jouan Dufournel I,Bronner G,Salter I,Agogué H,Mary I,Galand PE

    更新日期：2013-04-09 00:00:00

  • Water does not flow across the tight junctions of MDCK cell epithelium.

    abstract：:Although it has been known for decades that the tight junctions of fluid-transporting epithelia are leaky to ions, it has not been possible to determine directly whether significant transjunctional water movement also occurs. An optical microscopic technique was developed for the direct visualization of the flow veloc...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.11.6526

    authors： Kovbasnjuk O,Leader JP,Weinstein AM,Spring KR

    更新日期：1998-05-26 00:00:00

  • Presynaptic kainate receptors at hippocampal mossy fiber synapses.

    abstract：:Hippocampal mossy fibers, which are the axons of dentate granule cells, form powerful excitatory synapses onto the proximal dendrites of CA3 pyramidal cells. It has long been known that high-affinity binding sites for kainate, a glutamate receptor agonist, are present on mossy fibers. Here we summarize recent experime...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章,评审

    doi：10.1073/pnas.191351498

    authors： Schmitz D,Mellor J,Frerking M,Nicoll RA

    更新日期：2001-09-25 00:00:00

  • Four p53 DNA-binding domain peptides bind natural p53-response elements and bend the DNA.

    abstract：:Recent structural studies of the minimal core DNA-binding domain of p53 (p53DBD) complexed to a single consensus pentamer sequence and of the isolated p53 tetramerization domain have provided valuable insights into their functions, but many questions about their interacting roles and synergism remain unanswered. To be...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.19.8591

    authors： Balagurumoorthy P,Sakamoto H,Lewis MS,Zambrano N,Clore GM,Gronenborn AM,Appella E,Harrington RE

    更新日期：1995-09-12 00:00:00

  • An anatomy of normal and malignant gene expression.

    abstract：:A gene's expression pattern provides clues to its role in normal physiology and disease. To provide quantitative expression levels on a genome-wide scale, the Cancer Genome Anatomy Project (CGAP) uses serial analysis of gene expression (SAGE). Over 5 million transcript tags from more than 100 human cell types have bee...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.152324199

    authors： Boon K,Osorio EC,Greenhut SF,Schaefer CF,Shoemaker J,Polyak K,Morin PJ,Buetow KH,Strausberg RL,De Souza SJ,Riggins GJ

    更新日期：2002-08-20 00:00:00

  • Humans incorporate attention-dependent uncertainty into perceptual decisions and confidence.

    abstract：:Perceptual decisions are better when they take uncertainty into account. Uncertainty arises not only from the properties of sensory input but also from cognitive sources, such as different levels of attention. However, it is unknown whether humans appropriately adjust for such cognitive sources of uncertainty during p...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1717720115

    authors： Denison RN,Adler WT,Carrasco M,Ma WJ

    更新日期：2018-10-23 00:00:00

  • Switch from Myc/Max to Mad1/Max binding and decrease in histone acetylation at the telomerase reverse transcriptase promoter during differentiation of HL60 cells.

    abstract：:Recent evidence suggests that the Myc and Mad1 proteins are implicated in the regulation of the gene encoding the human telomerase reverse transcriptase (hTERT), the catalytic subunit of telomerase. We have analyzed the in vivo interaction between endogenous c-Myc and Mad1 proteins and the hTERT promoter in HL60 cells...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.071043198

    authors： Xu D,Popov N,Hou M,Wang Q,Björkholm M,Gruber A,Menkel AR,Henriksson M

    更新日期：2001-03-27 00:00:00

  • High-resolution repertoire analysis reveals a major bystander activation of Tfh and Tfr cells.

    abstract：:T follicular helper (Tfh) and regulatory (Tfr) cells are terminally differentiated cells found in germinal centers (GCs), specialized secondary lymphoid organ structures dedicated to antibody production. As such, follicular T (Tfol) cells are supposed to be specific for immunizing antigens, which has been reported for...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1808594115

    authors： Ritvo PG,Saadawi A,Barennes P,Quiniou V,Chaara W,El Soufi K,Bonnet B,Six A,Shugay M,Mariotti-Ferrandiz E,Klatzmann D

    更新日期：2018-09-18 00:00:00

  • Circadian clock protein cryptochrome regulates the expression of proinflammatory cytokines.

    abstract：:Chronic sleep deprivation perturbs the circadian clock and increases susceptibility to diseases such as diabetes, obesity, and cancer. Increased inflammation is one of the common underlying mechanisms of these diseases, thus raising a hypothesis that circadian-oscillator components may regulate immune response. Here w...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1209965109

    authors： Narasimamurthy R,Hatori M,Nayak SK,Liu F,Panda S,Verma IM

    更新日期：2012-07-31 00:00:00

  • Histone deacetylase inhibitor treatment dramatically reduces cholesterol accumulation in Niemann-Pick type C1 mutant human fibroblasts.

    abstract：:Niemann-Pick type C (NPC) disease is predominantly caused by mutations in the NPC1 protein that affect intracellular cholesterol trafficking and cause accumulation of unesterified cholesterol and other lipids in lysosomal storage organelles. We report the use of a series of small molecule histone deacetylase (HDAC) in...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1014890108

    authors： Pipalia NH,Cosner CC,Huang A,Chatterjee A,Bourbon P,Farley N,Helquist P,Wiest O,Maxfield FR

    更新日期：2011-04-05 00:00:00

  • Regulatory CD8 T cells that recognize Qa-1 expressed by CD4 T-helper cells inhibit rejection of heart allografts.

    abstract：:Induction of longstanding immunologic tolerance is essential for survival of transplanted organs and tissues. Despite recent advances in immunosuppression protocols, allograft damage inflicted by antibody specific for donor organs continues to represent a major obstacle to graft survival. Here we report that activatio...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1918950117

    authors： Choi JY,Eskandari SK,Cai S,Sulkaj I,Assaker JP,Allos H,AlHaddad J,Muhsin SA,Alhussain E,Mansouri A,Yeung MY,Seelen MAJ,Kim HJ,Cantor H,Azzi JR

    更新日期：2020-03-17 00:00:00

  • Cellular adaptation to opiates alters ion-channel mRNA levels.

    abstract：:The chronic use of several drugs, including opiates, results in the stereotypical behaviors characteristic of addiction. Alterations in gene expression have been associated with the use of these addictive drugs. Previous studies, however, have been limited to describing changes in amounts of individual mRNAs from sing...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.1.385

    authors： Mackler SA,Eberwine JH

    更新日期：1994-01-04 00:00:00

  • An approach to membrane protein structure without crystals.

    abstract：:The lactose permease of Escherichia coli catalyzes coupled translocation of galactosides and H(+) across the cell membrane. It is the best-characterized member of the Major Facilitator Superfamily, a related group of membrane proteins with 12 transmembrane domains that mediate transport of various substrates across ce...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.182552199

    authors： Sorgen PL,Hu Y,Guan L,Kaback HR,Girvin ME

    更新日期：2002-10-29 00:00:00

  • The compression hypothesis and temporal resource partitioning.

    abstract：:Contingency models of feeding compare the energy per unit time gained from utilizing a resource unit of a particular kind (food types, habitat patches, time periods) against that energy/time expected if the unit is skipped. Optimally, an animal should reject the particular unit if and only if the former energy/time is...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.71.10.4169

    authors： Schoener TW

    更新日期：1974-10-01 00:00:00

  • The Sca-1 cell surface marker enriches for a prostate-regenerating cell subpopulation that can initiate prostate tumorigenesis.

    abstract：:Sca-1 (stem cell antigen-1) enriches for murine prostate cells capable of regenerating tubular structures containing basal and luminal cell lineages in a dissociated cell prostate regeneration system. Sca-1(+) fractions are enriched for cells at the G(0) stage of the cell cycle, and Sca-1(+) cells cluster in the proxi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0502320102

    authors： Xin L,Lawson DA,Witte ON

    更新日期：2005-05-10 00:00:00

  • DNA length, bending, and twisting constraints on IS50 transposition.

    abstract：:Transposition is a multistep process in which a transposable element DNA sequence moves from its original genetic location to a new site. Early steps in this process include the formation of a transposition complex in which the end sequences of the transposable element are brought together in a structurally precise fa...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.23.10834

    authors： Goryshin IYu,Kil YV,Reznikoff WS

    更新日期：1994-11-08 00:00:00

  • Alternative splicing of agrin regulates its binding to heparin alpha-dystroglycan, and the cell surface.

    abstract：:Agrin is a basal lamina molecule that directs key events in postsynaptic differentiation, most notably the aggregation of acetylcholine receptors (AChRs) on the muscle cell surface. Agrin's AChR clustering activity is regulated by alternative mRNA splicing. Agrin splice forms having inserts at two sites (y and z) in t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.14.7369

    authors： O'Toole JJ,Deyst KA,Bowe MA,Nastuk MA,McKechnie BA,Fallon JR

    更新日期：1996-07-09 00:00:00