当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Alternative splicing of agrin regulates its binding to heparin alpha-dystroglycan, and the cell surface.

Alternative splicing of agrin regulates its binding to heparin alpha-dystroglycan, and the cell surface.

Abstract：

:Agrin is a basal lamina molecule that directs key events in postsynaptic differentiation, most notably the aggregation of acetylcholine receptors (AChRs) on the muscle cell surface. Agrin's AChR clustering activity is regulated by alternative mRNA splicing. Agrin splice forms having inserts at two sites (y and z) in the C-terminal region are highly active, but isoforms lacking these inserts are weakly active. The biochemical consequences of this alternative splicing are unknown. Here, the binding of four recombinant agrin isoforms to heparin, to alpha-dystroglycan (a component of an agrin receptor), and to myoblasts was tested. The presence of a four-amino acid insert at the y site is necessary and sufficient to confer heparin binding ability to agrin. Moreover, the binding of agrin to alpha-dystroglycan is inhibited by heparin when this insert is present. Agrin binding to the cell surface showed analogous properties: heparin inhibits the binding of only those agrin isoforms containing this four-amino acid insert. The results show that alternative splicing of agrin regulates its binding to heparin and suggest that agrin's interaction with alpha-dystroglycan may be modulated by cell surface glycosaminoglycans in an isoform-dependent manner.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

O'Toole JJ,Deyst KA,Bowe MA,Nastuk MA,McKechnie BA,Fallon JR

doi

10.1073/pnas.93.14.7369

subject

Has Abstract

pub_date

1996-07-09 00:00:00

pages

7369-74

issue

14

eissn

0027-8424

issn

1091-6490

journal_volume

93

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Human cytomegalovirus latency alters the cellular secretome, inducing cluster of differentiation (CD)4+ T-cell migration and suppression of effector function.

    abstract：:After primary infection, human cytomegalovirus (HCMV) persists as a life-long latent infection, with host immunosuppression often resulting in clinical reactivation. During lytic infection, major changes in the expression of secreted cellular proteins (the secretome) occur that have profound effects on host-cell inter...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1204836109

    authors： Mason GM,Poole E,Sissons JG,Wills MR,Sinclair JH

    更新日期：2012-09-04 00:00:00

  • Dynamic updating of hippocampal object representations reflects new conceptual knowledge.

    abstract：:Concepts organize the relationship among individual stimuli or events by highlighting shared features. Often, new goals require updating conceptual knowledge to reflect relationships based on different goal-relevant features. Here, our aim is to determine how hippocampal (HPC) object representations are organized and ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1614048113

    authors： Mack ML,Love BC,Preston AR

    更新日期：2016-11-15 00:00:00

  • Hv1 proton channels are required for high-level NADPH oxidase-dependent superoxide production during the phagocyte respiratory burst.

    abstract：:Granulocytes generate a "respiratory burst" of NADPH oxidase-dependent superoxide anion (O(2)(-*)) production that is required for efficient clearance of bacterial pathogens. Hv1 mediates a voltage-gated H(+) channel activity that is proposed to serve a charge-balancing role in granulocytic phagocytes such as neutroph...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0902761106

    authors： Ramsey IS,Ruchti E,Kaczmarek JS,Clapham DE

    更新日期：2009-05-05 00:00:00

  • Cell sorting-out is modulated by both the specificity and amount of different cell adhesion molecules (CAMs) expressed on cell surfaces.

    abstract：:Cell adhesion molecules (CAMs) are cell surface glycoproteins that may play a variety of roles in morphogenesis and histogenesis, particularly in defining borders of discrete cell populations. To examine the influence of CAM expression on such cell segregation events in vitro, we have transfected cells with cDNAs codi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.86.18.7043

    authors： Friedlander DR,Mège RM,Cunningham BA,Edelman GM

    更新日期：1989-09-01 00:00:00

  • "Alternating B-DNA" conformation for the oligo(dG-dC) duplex in high-salt solution.

    abstract：:The high resolution 1H and 31P NMR spectra of the (dG-dC)8 duplex have been recorded in low- and high-salt solutions in order to evaluate the structural aspects of the salt-induced transition of oligo(dG-dC) in solution [Pohl, F. M. & Jovin, T. M. (1972) J. Mol. Biol. 67, 375-396]. The NMR data require that the (dG-d...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.76.6.2508

    authors： Patel DJ,Canuel LL,Pohl FM

    更新日期：1979-06-01 00:00:00

  • Human disturbance increases trophic niche overlap in terrestrial carnivore communities.

    abstract：:Animal foraging and competition are defined by the partitioning of three primary niche axes: space, time, and resources. Human disturbance is rapidly altering the spatial and temporal niches of animals, but the impact of humans on resource consumption and partitioning-arguably the most important niche axis-is poorly u...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2012774117

    authors： Manlick PJ,Pauli JN

    更新日期：2020-10-27 00:00:00

  • Faithful representation of similarities among three-dimensional shapes in human vision.

    abstract：:Efficient and reliable classification of visual stimuli requires that their representations reside a low-dimensional and, therefore, computationally manageable feature space. We investigated the ability of the human visual system to derive such representations from the sensory input-a highly nontrivial task, given the...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.21.12046

    authors： Cutzu F,Edelman S

    更新日期：1996-10-15 00:00:00

  • Characterization of inhibitory and stimulatory forms of the murine natural killer cell receptor 2B4.

    abstract：:The receptor 2B4 belongs to the Ig superfamily and is found on the surface of all murine natural killer (NK) cells as well as T cells displaying non-MHC-restricted cytotoxicity. Previous studies have suggested that 2B4 is an activating molecule because cross-linking of this receptor results in increased cytotoxicity a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.7.3870

    authors： Schatzle JD,Sheu S,Stepp SE,Mathew PA,Bennett M,Kumar V

    更新日期：1999-03-30 00:00:00

  • Characterization of capsaicin synthase and identification of its gene (csy1) for pungency factor capsaicin in pepper (Capsicum sp.).

    abstract：:Capsaicin is a unique alkaloid of the plant kingdom restricted to the genus Capsicum. Capsaicin is the pungency factor, a bioactive molecule of food and of medicinal importance. Capsaicin is useful as a counterirritant, antiarthritic, analgesic, antioxidant, and anticancer agent. Capsaicin biosynthesis involves conden...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章,收录出版

    doi：10.1073/pnas.0605805103

    authors： Prasad BC,Kumar V,Gururaj HB,Parimalan R,Giridhar P,Ravishankar GA

    更新日期：2006-09-05 00:00:00

  • The structure of zetekitoxin AB, a saxitoxin analog from the Panamanian golden frog Atelopus zeteki: a potent sodium-channel blocker.

    abstract：:Bufonid anurans of the genus Atelopus contain both steroidal bufadienolides and various guanidinium alkaloids of the tetrodotoxin class. The former inhibit sodium-potassium ATPases, whereas the latter block voltage-dependent sodium channels. The structure of one guanidinium alkaloid, zetekitoxin AB, has remained a mys...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0400368101

    authors： Yotsu-Yamashita M,Kim YH,Dudley SC Jr,Choudhary G,Pfahnl A,Oshima Y,Daly JW

    更新日期：2004-03-30 00:00:00

  • Lysosomal enzyme tripeptidyl peptidase 1 destabilizes fibrillar Aβ by multiple endoproteolytic cleavages within the β-sheet domain.

    abstract：:Accumulation of amyloid-beta (Aβ), which is associated with Alzheimer's disease, can be caused by excess production or insufficient clearance. Because of its β-sheet structure, fibrillar Aβ is resistant to proteolysis, which would contribute to slow degradation of Aβ plaques in vivo. Fibrillar Aβ can be internalized b...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1719808115

    authors： Solé-Domènech S,Rojas AV,Maisuradze GG,Scheraga HA,Lobel P,Maxfield FR

    更新日期：2018-02-13 00:00:00

  • Huntingtin-associated protein (HAP1): discrete neuronal localizations in the brain resemble those of neuronal nitric oxide synthase.

    abstract：:Huntington disease stems from a mutation of the protein huntingtin and is characterized by selective loss of discrete neuronal populations in the brain. Despite a massive loss of neurons in the corpus striatum, NO-generating neurons are intact. We recently identified a brain-specific protein that associates with hunti...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.10.4839

    authors： Li XJ,Sharp AH,Li SH,Dawson TM,Snyder SH,Ross CA

    更新日期：1996-05-14 00:00:00

  • Enhanced hepatitis C virus genome replication and lipid accumulation mediated by inhibition of AMP-activated protein kinase.

    abstract：:Hepatitis C virus (HCV) infection is associated with dysregulation of both lipid and glucose metabolism. As well as contributing to viral replication, these perturbations influence the pathogenesis associated with the virus, including steatosis, insulin resistance, and type 2 diabetes. AMP-activated protein kinase (AM...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0912426107

    authors： Mankouri J,Tedbury PR,Gretton S,Hughes ME,Griffin SD,Dallas ML,Green KA,Hardie DG,Peers C,Harris M

    更新日期：2010-06-22 00:00:00

  • Insulin resistance in skeletal muscles of caveolin-3-null mice.

    abstract：:Type 2 diabetes is preceded by the development of insulin resistance, in which the action of insulin is impaired, largely in skeletal muscles. Caveolin-3 (Cav3) is a muscle-specific subtype of caveolin, an example of a scaffolding protein found within membranes. Cav is also known as growth signal inhibitor, although i...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0402053101

    authors： Oshikawa J,Otsu K,Toya Y,Tsunematsu T,Hankins R,Kawabe J,Minamisawa S,Umemura S,Hagiwara Y,Ishikawa Y

    更新日期：2004-08-24 00:00:00

  • Three chemokine receptors cooperatively regulate homing of hematopoietic progenitors to the embryonic mouse thymus.

    abstract：:The thymus lacks self-renewing hematopoietic cells, and thymopoiesis fails rapidly when the migration of progenitor cells to the thymus ceases. Hence, the process of thymus homing is an essential step for T-cell development and cellular immunity. Despite decades of research, the molecular details of thymus homing have...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1016428108

    authors： Calderón L,Boehm T

    更新日期：2011-05-03 00:00:00

  • Cyclophilin catalyzes protein folding in yeast mitochondria.

    abstract：:Cyclophilins are a family of ubiquitous proteins that are the intracellular target of the immunosuppressant drug cyclosporin A. Although cyclophilins catalyze peptidylprolyl cis-trans isomerization in vitro, it has remained open whether they also perform this function in vivo. Here we show that Cpr3p, a cyclophilin in...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.14.6319

    authors： Matouschek A,Rospert S,Schmid K,Glick BS,Schatz G

    更新日期：1995-07-03 00:00:00

  • Genome-wide CRISPR screens reveal a specific ligand for the glycan-binding immune checkpoint receptor Siglec-7.

    abstract：:Glyco-immune checkpoint receptors, molecules that inhibit immune cell activity following binding to glycosylated cell-surface antigens, are emerging as attractive targets for cancer immunotherapy. Defining biologically relevant ligands that bind and activate such receptors, however, has historically been a significant...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2015024118

    authors： Wisnovsky S,Möckl L,Malaker SA,Pedram K,Hess GT,Riley NM,Gray MA,Smith BAH,Bassik MC,Moerner WE,Bertozzi CR

    更新日期：2021-02-02 00:00:00

  • Antennal transcriptome of Manduca sexta.

    abstract：:In recent years, considerable progress has been made in understanding the molecular mechanisms underlying olfaction in insects. Because of the diverse nature of the gene families involved, this process has largely relied on genomic data. As a consequence, studies have focused on a small subset of species with extensiv...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1017963108

    authors： Grosse-Wilde E,Kuebler LS,Bucks S,Vogel H,Wicher D,Hansson BS

    更新日期：2011-05-03 00:00:00

  • Eukaryotic Mr 83,000 heat shock protein has a homologue in Escherichia coli.

    abstract：:We have isolated a gene from Escherichia coli homologous to the gene encoding the Mr 83,000 Drosophila heat shock protein (hsp83). In E. coli the protein homologous to hsp83 is a heat shock protein called C62.5. The predicted amino acid sequence of C62.5 is 41% and 42% identical to the Drosophila and human hsp83 prote...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.15.5177

    authors： Bardwell JC,Craig EA

    更新日期：1987-08-01 00:00:00

  • Regulatory T cells control tolerogenic versus autoimmune response to sperm in vasectomy.

    abstract：:Vasectomy is a well accepted global contraceptive approach frequently associated with epididymal granuloma and sperm autoantibody formation. To understand the long-term sequelae of vasectomy, we investigated the early immune response in vasectomized mice. Vasectomy leads to rapid epithelial cell apoptosis and necrosis...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1017615108

    authors： Wheeler K,Tardif S,Rival C,Luu B,Bui E,Del Rio R,Teuscher C,Sparwasser T,Hardy D,Tung KS

    更新日期：2011-05-03 00:00:00

  • Bayesian estimation of SARS-CoV-2 prevalence in Indiana by random testing.

    abstract：:From 25 to 29 April 2020, the state of Indiana undertook testing of 3,658 randomly chosen state residents for the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus, the agent causing COVID-19 disease. This was the first statewide randomized study of COVID-19 testing in the United States. Both PC...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2013906118

    authors： Yiannoutsos CT,Halverson PK,Menachemi N

    更新日期：2021-02-02 00:00:00

  • Identification of slow correlated motions in proteins using residual dipolar and hydrogen-bond scalar couplings.

    abstract：:Despite their importance for biological activity, slower molecular motions beyond the nanosecond range remain poorly understood. We have assembled an unprecedented set of experimental NMR data, comprising up to 27 residual dipolar couplings per amino acid, to define the nature and amplitude of backbone motion in prote...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0505129102

    authors： Bouvignies G,Bernadó P,Meier S,Cho K,Grzesiek S,Brüschweiler R,Blackledge M

    更新日期：2005-09-27 00:00:00

  • Selectivity of ORC binding sites and the relation to replication timing, fragile sites, and deletions in cancers.

    abstract：:The origin recognition complex (ORC) binds sites from which DNA replication is initiated. We address ORC binding selectivity in vivo by mapping ∼52,000 ORC2 binding sites throughout the human genome. The ORC binding profile is broader than those of sequence-specific transcription factors, suggesting that ORC is not bo...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1609060113

    authors： Miotto B,Ji Z,Struhl K

    更新日期：2016-08-16 00:00:00

  • A cytochrome b5 is required for full activity of flavonoid 3', 5'-hydroxylase, a cytochrome P450 involved in the formation of blue flower colors.

    abstract：:The substitution pattern of anthocyanin pigments is a main determinant of flower color. Flavonoid 3',5'-hydroxylase (F3'5'H) is a cytochrome P450 enzyme (Cyt P450) that catalyzes the 3', 5'-hydroxylation of dihydroflavonols, the precursors of purple anthocyanins. Species such as rose and carnation lack F3'5'H activity...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.2.778

    authors： de Vetten N,ter Horst J,van Schaik HP,de Boer A,Mol J,Koes R

    更新日期：1999-01-19 00:00:00

  • The su(Hw) protein bound to gypsy sequences in one chromosome can repress enhancer-promoter interactions in the paired gene located in the other homolog.

    abstract：:The suppressor of Hairy-wing [su(Hw)] protein exerts a polar effect on gene expression by repressing the function of transcriptional enhancers located distally from the promoter with respect to the location of su(Hw) binding sequences. The directionality of this effect suggests that the su(Hw) protein specifically int...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.11.5184

    authors： Georgiev PG,Corces VG

    更新日期：1995-05-23 00:00:00

  • Adaptive response due to changes in gene regulation: a study with Drosophila.

    abstract：:In spite of the critical role of the process of adaptation in evolution, there are few detailed studies of the genotypic and molecular basis of the process. Drosophila melanogaster flies selected for increased tolerance to ethanol exhibited higher levels of alcohol dehydrogenase (alcohol:NAD+ oxidoreductase; EC 1.1.1....

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.74.10.4562

    authors： McDonald JF,Chambers GK,David J,Ayala FJ

    更新日期：1977-10-01 00:00:00

  • An essential role for the phosphatidylinositol transfer protein in the scission of coatomer-coated vesicles from the trans-Golgi network.

    abstract：:We identified the phosphatidylinositol transfer protein (PITP) as being responsible for a powerful latent, nucleotide-independent, Golgi-vesiculating activity that is present in the cytosol but is only manifested as an uncontrolled activity in a cytosolic protein subfraction, in which it is separated from regulatory c...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.19.11181

    authors： Simon JP,Morimoto T,Bankaitis VA,Gottlieb TA,Ivanov IE,Adesnik M,Sabatini DD

    更新日期：1998-09-15 00:00:00

  • New evidence on the impact of sustained exposure to air pollution on life expectancy from China's Huai River Policy.

    abstract：:This paper finds that a 10-μg/m3 increase in airborne particulate matter [particulate matter smaller than 10 μm (PM10)] reduces life expectancy by 0.64 years (95% confidence interval = 0.21-1.07). This estimate is derived from quasiexperimental variation in PM10 generated by China's Huai River Policy, which provides f...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1616784114

    authors： Ebenstein A,Fan M,Greenstone M,He G,Zhou M

    更新日期：2017-09-26 00:00:00

  • H2O2-induced uncoupling of bovine lens Na+,K+-ATPase.

    abstract：:A 1-hr exposure of bovine lenses in organ culture to H2O2 concentrations in the range found in the aqueous fluid of patients with cataracts inhibits 86Rb+ influx. At 1 mM H2O2, complete inhibition was observed and further investigated. Membrane permeability is slightly decreased. Although lactate concentrations increa...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.80.7.2044

    authors： Garner WH,Garner MH,Spector A

    更新日期：1983-04-01 00:00:00

  • Identification and characterization of recombinant plasmids carrying the complete qa gene cluster from Neurospora crassa including the qa-1+ regulatory gene.

    abstract：:The early reactions in the catabolism of quinic acid in Neurospora crassa are controlled by at least four genes which are clustered on linkage group VII. Three of the loci (qa-2, qa-4, and qa-3) encode enzymes that convert quinic acid to protocatechuic acid. The fourth gene (qa-1) encodes a positive regulatory protein...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.78.8.5086

    authors： Schweizer M,Case ME,Dykstra CC,Giles NH,Kushner SR

    更新日期：1981-08-01 00:00:00