Lysosomal enzyme tripeptidyl peptidase 1 destabilizes fibrillar Aβ by multiple endoproteolytic cleavages within the β-sheet domain.

abstract：:The Wiskott-Aldrich syndrome protein (WASp) plays a major role in coupling T cell antigen receptor (TCR) stimulation to induction of actin cytoskeletal changes required for T cell activation. Here, we report that WASp inducibly binds the sorting nexin 9 (SNX9) in T cells and that WASp, SNX9, p85, and CD28 colocalize w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Badour K,McGavin MK,Zhang J,Freeman S,Vieira C,Filipp D,Julius M,Mills GB,Siminovitch KA

更新日期：2007-01-30 00:00:00

abstract：:Echoviruses are enteroviruses that belong to Picornaviridae. Many echoviruses use decay-accelerating factor (DAF) as their cellular receptor. DAF is a glycosylphosphatidyl inositol-anchored complement regulatory protein found on most cell surfaces. It functions to protect cells from complement attack. The cryo-electro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： He Y,Lin F,Chipman PR,Bator CM,Baker TS,Shoham M,Kuhn RJ,Medof ME,Rossmann MG

更新日期：2002-08-06 00:00:00

abstract：:In recent years, the field of psychology has begun to conduct replication tests on a large scale. Here, we show that "replicator degrees of freedom" make it far too easy to obtain and publish false-negative replication results, even while appearing to adhere to strict methodological standards. Specifically, using data...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bryan CJ,Yeager DS,O'Brien JM

更新日期：2019-12-17 00:00:00

abstract：:Autoimmunity often involves the abnormal targeting of self-antigens by antibodies, leading to tissue destruction and other pathologies. This process could potentially be disrupted by small ligands that bind specifically to autoantibodies and inhibit their interaction with the target antigen. Here we report the identif...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Doudna JA,Cech TR,Sullenger BA

更新日期：1995-03-14 00:00:00

abstract：:We have analyzed the spectra of UV-induced mutations in the lacI gene of a wild-type and an mfd strain of Escherichia coli. mfd strains have been recently proposed to be deficient in a factor coupling DNA repair and transcription. Analysis of UV-induced mutations occurring at adjacent pyrimidines showed that mutations...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oller AR,Fijalkowska IJ,Dunn RL,Schaaper RM

更新日期：1992-11-15 00:00:00

abstract：:The diabetogenic agent alloxan exerts a preferential cytotoxic effect on the pancreatic B cell. The determinants of such a tissue specificity were investigated. Alloxan accumulated rapidly in liver and pancreatic islets but much more slowly in muscle. The activity of glutathione peroxidase and the resistance to exogen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Malaisse WJ,Malaisse-Lagae F,Sener A,Pipeleers DG

更新日期：1982-02-01 00:00:00

abstract：:The last 2 decades have seen discoveries in highly excited states of atoms and molecules of phenomena that are qualitatively different from the "planetary" model of the atom, and the near-rigid model of molecules, characteristic of these systems in their low-energy states. A unified view is emerging in terms of approx...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kellman ME

更新日期：1996-12-10 00:00:00

abstract：:Due to the small size of a nanoconfinement, the property of water contained inside is rather challenging to probe. Herein, we measured the amount of water molecules released during the folding of individual G-quadruplex and i-motif structures, from which water activities are estimated in the DNA nanocages prepared by ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jonchhe S,Pandey S,Emura T,Hidaka K,Hossain MA,Shrestha P,Sugiyama H,Endo M,Mao H

更新日期：2018-09-18 00:00:00

abstract：:There is considerable evidence that links the activation of cellular genes to oncogenesis. We previously reported that structural rearrangements in the cellular oncogene c-erbB correlate with the development of erythroblastosis induced by avian leukosis virus (ALV). c-erbB recently has been shown to be related to the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Raines MA,Lewis WG,Crittenden LB,Kung HJ

更新日期：1985-04-01 00:00:00

abstract：:Rhomboid domain-containing protein 3 (Rhbdd3), which belongs to a family of proteins with rhomboid domain, is widely expressed in immune cells; however, the roles of the Rhbdd members, including Rhbdd3, in immunity remain unknown. Natural killer (NK) cells are critical for host immune defense and also can mediate infl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu J,Liu S,Xia M,Xu S,Wang C,Bao Y,Jiang M,Wu Y,Xu T,Cao X

更新日期：2013-05-07 00:00:00

abstract：:Two genes have a synthetically lethal relationship when the silencing or inhibiting of 1 gene is only lethal in the context of a mutation or activation of the second gene. This situation offers an attractive therapeutic strategy, as inhibition of such a gene will only trigger cell death in tumor cells with an activate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Molenaar JJ,Ebus ME,Geerts D,Koster J,Lamers F,Valentijn LJ,Westerhout EM,Versteeg R,Caron HN

更新日期：2009-08-04 00:00:00

abstract：:Pyrroline-carboxy-lysine (Pcl) is a demethylated form of pyrrolysine that is generated by the pyrrolysine biosynthetic enzymes when the growth media is supplemented with D-ornithine. Pcl is readily incorporated by the unmodified pyrrolysyl-tRNA/tRNA synthetase pair into proteins expressed in Escherichia coli and in ma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ou W,Uno T,Chiu HP,Grünewald J,Cellitti SE,Crossgrove T,Hao X,Fan Q,Quinn LL,Patterson P,Okach L,Jones DH,Lesley SA,Brock A,Geierstanger BH

更新日期：2011-06-28 00:00:00

abstract：:cDNAs made from polyadenylylated RNAs of the mouse lens were cloned by the G.C tailing procedure in the bacterial plasmid pBR322. Four recombinant DNAs containing gamma-crystallin sequences were identified by hybrid selection and translation. Sequence analysis of the in vivo-labeled gamma-crystallin polypeptides that ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shinohara T,Robinson EA,Appella E,Piatigorsky J

更新日期：1982-05-01 00:00:00

abstract：:Amphibians are a bellwether for environmental degradation, even in natural ecosystems such as Yellowstone National Park in the western United States, where species have been actively protected longer than anywhere else on Earth. We document that recent climatic warming and resultant wetland desiccation are causing sev...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McMenamin SK,Hadly EA,Wright CK

更新日期：2008-11-04 00:00:00

abstract：:Bacterial mutants have been isolated, called groN, that block phage development by interference with the action of the product of the phage N gene. lambdatrp phages, which depend on the N product for the synthesis of tryptophan enzymes, do not make these enzymes in groN bacteria. Two type of phage mutants have been is...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Georgopoulos CP

更新日期：1971-12-01 00:00:00

abstract：:A screening procedure based on solvent extraction with minimal clean up followed by examination with negative chemical ionization mass spectrometry has been developed for organochlorine residues and related compounds with masses greater than 130 daltons. The procedure has been made quantitative for analysis of pentach...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dougherty RC,Piotrowska K

更新日期：1976-06-01 00:00:00

abstract：:A mutation in the Escherichia coli dsbA gene (coding for a periplasmic disulfide oxidoreductase) reduces the rate of disulfide bond formation in the enzyme pullulanase and also reduces the rate at which the enzyme is secreted to the cell surface, as measured by protease accessibility. The enzyme did not become proteas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pugsley AP

更新日期：1992-12-15 00:00:00

abstract：:Although the binding change mechanism of rotary catalysis by which F1-ATPase hydrolyzes ATP has been supported by equilibrium, kinetic, and structural observations, many questions concerning the function remain unanswered. Because of the importance of this enzyme, the search for a full understanding of its mechanism i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gao YQ,Yang W,Marcus RA,Karplus M

更新日期：2003-09-30 00:00:00

abstract：:The formation of complex but highly organized neural circuits requires interactions between neurons and glia. During the assembly of the Drosophila olfactory circuit, 50 olfactory receptor neuron (ORN) classes and 50 projection neuron (PN) classes form synaptic connections in 50 glomerular compartments in the antennal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu B,Li J,Chou YH,Luginbuhl D,Luo L

更新日期：2017-07-18 00:00:00

abstract：:Cyclization/cleavage of the 2-(p-nitrophenyl) phosphate ester of propylene glycol is catalyzed by imidazole and, much more effectively, by Zn2+ with imidazole. In the latter case, the mechanism involves simultaneous Lewis acid/base catalysis. Similar Zn2+ and imidazole catalysis of cyclization/cleavage is seen with th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Breslow R,Huang DL,Anslyn E

更新日期：1989-03-01 00:00:00

abstract：:The reactions catalyzed by Mo enzymes each find the product differing from the substrate by two electrons and two protons (or some multiple thereof). The coordination chemistry of Mo suggests that there is a distinct relationship between acid-base and redox properties of Mo complexes, and that a coupled electron-proto...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stiefel EI

更新日期：1973-04-01 00:00:00

abstract：:The basal ganglia are critical for selecting actions and evaluating their outcome. Although the circuitry for selection is well understood, how these nuclei evaluate the outcome of actions is unknown. Here, we show in lamprey that a separate evaluation circuit, which regulates the habenula-projecting globus pallidus (...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stephenson-Jones M,Kardamakis AA,Robertson B,Grillner S

更新日期：2013-09-17 00:00:00

abstract：:The SecY protein of Escherichia coli is an integral membrane component of the protein export apparatus. Suppressor mutations in the secY gene (prlA alleles) have been isolated that restore the secretion of precursor proteins with defective signal sequences. These mutations have never been shown to affect the transloca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nouwen N,de Kruijff B,Tommassen J

更新日期：1996-06-11 00:00:00

abstract：:The with-no-lysine (K) (WNK) kinases are an atypical family of protein kinases that regulate ion transport across cell membranes. Mutations that result in their overexpression cause hypertension-related disorders in humans. Of the four mammalian WNKs, only WNK1 is expressed throughout the body. We report that WNK1 inh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gallolu Kankanamalage S,Lee AY,Wichaidit C,Lorente-Rodriguez A,Shah AM,Stippec S,Whitehurst AW,Cobb MH

更新日期：2016-12-13 00:00:00

abstract：:An algorithm for protein engineering, termed recursive ensemble mutagenesis, has been developed to produce diverse populations of phenotypically related mutants whose members differ in amino acid sequence. This method uses a feedback mechanism to control successive rounds of combinatorial cassette mutagenesis. Startin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arkin AP,Youvan DC

更新日期：1992-08-15 00:00:00

abstract：:Reported effects of anti-CD154 treatment on autoimmunity, alloreactivity, and inflammatory events mediated by macrophages and endothelial cells indicated that it might be an ideal agent for the prevention of intrahepatic islet allograft failure. This hypothesis was tested in MHC-mismatched rhesus monkeys. Transplantat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kenyon NS,Chatzipetrou M,Masetti M,Ranuncoli A,Oliveira M,Wagner JL,Kirk AD,Harlan DM,Burkly LC,Ricordi C

更新日期：1999-07-06 00:00:00

abstract：:An antigen histochemically localized in the nuclei and cytoplasmic granules of normal and leukemic human myeloid cells has been identified as myeloperoxidase (MPO; EC 1.11.1.7). The localization and amount of the enzyme was determined by using a murine monoclonal antibody designated H-43-5 raised against nuclear prote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murao S,Stevens FJ,Ito A,Huberman E

更新日期：1988-02-01 00:00:00

abstract：:The recent development of HIV-1 lentiviral vectors is especially useful for gene transfer because they achieve efficient integration into nondividing cell genomes and successful long-term expression of the transgene. These attributes make the vector useful for gene delivery, mutagenesis, and other applications in mamm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lai Z,Han I,Park M,Brady RO

更新日期：2002-03-19 00:00:00

abstract：:Single-cell RNA sequencing (scRNA-seq) enables the quantification of each gene's expression distribution across cells, thus allowing the assessment of the dispersion, nonzero fraction, and other aspects of its distribution beyond the mean. These statistical characterizations of the gene expression distribution are cri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang J,Huang M,Torre E,Dueck H,Shaffer S,Murray J,Raj A,Li M,Zhang NR

更新日期：2018-07-10 00:00:00

abstract：:Germ-line missense mutations of the receptor-like tyrosine kinase ret are the causative genetic event of the multiple endocrine neoplasia (MEN) type 2A and type 2B syndromes and of the familial medullary thyroid carcinoma. We have used the rat pheochromocytoma cell line, PC12, as a model system to investigate the mech...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Califano D,D'Alessio A,Colucci-D'Amato GL,De Vita G,Monaco C,Santelli G,Di Fiore PP,Vecchio G,Fusco A,Santoro M,de Franciscis V

更新日期：1996-07-23 00:00:00