Evidence for presence of an internal thiolester bond in third component of human complement.

abstract：:We demonstrate how a conventional confocal spinning-disk (CSD) microscope can be converted into a doubly resolving image scanning microscopy (ISM) system without changing any part of its optical or mechanical elements. Making use of the intrinsic properties of a CSD microscope, we illuminate stroboscopically, generati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schulz O,Pieper C,Clever M,Pfaff J,Ruhlandt A,Kehlenbach RH,Wouters FS,Großhans J,Bunt G,Enderlein J

更新日期：2013-12-24 00:00:00

abstract：:More than two decades ago, the activation mechanism for the membrane-bound photoreceptor and prototypical G protein-coupled receptor (GPCR) rhodopsin was uncovered. Upon light-induced changes in ligand-receptor interaction, movement of specific transmembrane helices within the receptor opens a crevice at the cytoplasm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Van Eps N,Altenbach C,Caro LN,Latorraca NR,Hollingsworth SA,Dror RO,Ernst OP,Hubbell WL

更新日期：2018-03-06 00:00:00

abstract：:Apoptotic cells contain nuclear autoantigens that may initiate a systemic autoimmune response. To explore the mechanism of antibody binding to apoptotic cells, 3H9, a murine autoantibody with dual specificity for phospholipids and DNA, was used. H chain mutants of 3H9 were constructed, expressed as single-chain Fv (sc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cocca BA,Seal SN,D'Agnillo P,Mueller YM,Katsikis PD,Rauch J,Weigert M,Radic MZ

更新日期：2001-11-20 00:00:00

abstract：:Tumor-associated myeloid cells (TAMCs) are key drivers of immunosuppression in the tumor microenvironment, which profoundly impedes the clinical response to immune-dependent and conventional therapeutic modalities. As a hallmark of glioblastoma (GBM), TAMCs are massively recruited to reach up to 50% of the brain tumor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang P,Miska J,Lee-Chang C,Rashidi A,Panek WK,An S,Zannikou M,Lopez-Rosas A,Han Y,Xiao T,Pituch KC,Kanojia D,Balyasnikova IV,Lesniak MS

更新日期：2019-11-19 00:00:00

abstract：:Rotaviruses are the single most common cause of fatal and severe childhood diarrheal illness worldwide (>125 million cases annually). Rotavirus shares structural and functional features with many viruses, such as the presence of segmented double-stranded RNA genomes selectively and tightly packed with a conserved numb...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Periz J,Celma C,Jing B,Pinkney JN,Roy P,Kapanidis AN

更新日期：2013-07-16 00:00:00

abstract：:Ceramide, a lipid mediator, has been most closely associated with antiproliferative activities. In this study, we examine the mechanism by which ceramide induces growth suppression and the role of the retinoblastoma gene product (Rb) in this process. Withdrawal of serum from the serum-dependent MOLT-4 cells resulted i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dbaibo GS,Pushkareva MY,Jayadev S,Schwarz JK,Horowitz JM,Obeid LM,Hannun YA

更新日期：1995-02-28 00:00:00

abstract：:Two regions of human thyrotropin (thyroid-stimulating hormone, TSH) receptor (TSHR) (residues 12-44 and 308-364) were selected on the basis that they exhibit no sequence resemblance to luteinizing hormone/chorionic gonadotropin receptor. Five synthetic overlapping peptides (12-30, 24-44, 308-328, 324-344, and 339-364)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Atassi MZ,Manshouri T,Sakata S

更新日期：1991-05-01 00:00:00

abstract：:The structure of the proline-specific aminopeptidase (EC 3.4.11.9) from Escherichia coli has been solved and refined for crystals of the native enzyme at a 2.0-A resolution, for a dipeptide-inhibited complex at 2.3-A resolution, and for a low-pH inactive form at 2.7-A resolution. The protein crystallizes as a tetramer...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wilce MC,Bond CS,Dixon NE,Freeman HC,Guss JM,Lilley PE,Wilce JA

更新日期：1998-03-31 00:00:00

abstract：:Transcription in eukaryotic nuclei is carried out by DNA-dependent RNA polymerases I, II, and III. Human RNA polymerase III (Pol III) transcribes small untranslated RNAs that include tRNAs, 5S RNA, U6 RNA, and some microRNAs. Increased Pol III transcription has been reported to accompany or cause cell transformation. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haurie V,Durrieu-Gaillard S,Dumay-Odelot H,Da Silva D,Rey C,Prochazkova M,Roeder RG,Besser D,Teichmann M

更新日期：2010-03-02 00:00:00

abstract：:Membrane-associated and periplasmic proteins of Agrobacterium tumefaciens C-58 were compared with those from avirulent (nontumrigenic) derivative strains by slab and two-dimensional gel electrophoresis. Two proteins (Per-I and Per-2), with a molecular weight of 37,500 and 37,300, respectively, were detected in the sup...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sonoki S,Kado CI

更新日期：1978-08-01 00:00:00

abstract：:The two large cytoplasmic domains (C1 and C2) of adenylyl cyclases (AC), when expressed separately and mixed together, reconstitute enzyme activity that can be regulated by various modulators. Therefore, we have used the C1 or its C1a subdomain and C2 regions from type I AC (ACI) and type V AC (ACV) to identify the re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wittpoth C,Scholich K,Yigzaw Y,Stringfield TM,Patel TB

更新日期：1999-08-17 00:00:00

abstract：:Expression of amino acid biosynthesis genes in bacteria is often repressed when abundant supplies of the cognate amino acid are available. Repression of the Bacillus subtilis lysC gene by lysine was previously shown to occur at the level of premature termination of transcription. In this study we show that lysine dire...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grundy FJ,Lehman SC,Henkin TM

更新日期：2003-10-14 00:00:00

abstract：:Bacterial virulence factors are typically surface-associated or secreted molecules that in Gram-negative bacteria must cross the outer membrane (OM). Protein translocation across the bacterial OM is not well understood. To elucidate this process we studied P pilus biogenesis in Escherichia coli. We present high-resolu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thanassi DG,Saulino ET,Lombardo MJ,Roth R,Heuser J,Hultgren SJ

更新日期：1998-03-17 00:00:00

abstract：:Using an FK506 affinity column to identify mammalian immunosuppressant-binding proteins, we identified an immunophilin with an apparent M(r) approximately 55,000, which we have named FKBP52. We used chemically determined peptide sequence and a computerized algorithm to search GenPept, the translated GenBank data base,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peattie DA,Harding MW,Fleming MA,DeCenzo MT,Lippke JA,Livingston DJ,Benasutti M

更新日期：1992-11-15 00:00:00

abstract：:Binding of a partial proteolytic digest by V8 enzyme of the yeast site-specific recombinase Flp to its target site gives rise to DNA-protein complexes that are smaller than those produced by the full-sized protein. The smallest of these complexes (occupancy of one peptide monomer per site) contains either one of two p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen JW,Evans BR,Yang SH,Teplow DB,Jayaram M

更新日期：1991-07-15 00:00:00

abstract：:Epilepsy is characterized by recurrent synchronizations of neuronal activity, which are both a cardinal clinical symptom and a debilitating phenomenon. Although the temporal dynamics of epileptiform synchronizations are well described at the macroscopic level using electrophysiological approaches, less is known about ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Feldt Muldoon S,Soltesz I,Cossart R

更新日期：2013-02-26 00:00:00

abstract：:Antibody specific for the native form of Chinese hamster hypoxanthine/guanine phosphoribosyltransferase (HPRT; IMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.8) was used to detect the synthesis of HPRT protein in a rabbit reticulocyte lysate translation system primed with mRNA from Chinese hamster tissues and c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Melton DW,Konecki DS,Ledbetter DH,Hejtmancik JF,Caskey CT

更新日期：1981-11-01 00:00:00

abstract：:Murine suppressor T-cell hybridoma cells (231F1) secrete not only bioactive glycosylation-inhibiting factor (GIF) but also an inactive peptide comparable to bioactive GIF peptide in its molecular size and reactivity with anti-GIF; the amino acid sequence of the inactive peptide is identical to that of the bioactive ho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakano T,Liu YC,Mikayama T,Watarai H,Taniguchi M,Ishizaka K

更新日期：1995-09-26 00:00:00

abstract：:Among yeasts that underwent whole-genome duplication (WGD), Kluyveromyces polysporus represents the lineage most distant from Saccharomyces cerevisiae. By sequencing the K. polysporus genome and comparing it with the S. cerevisiae genome using a likelihood model of gene loss, we show that these species diverged very s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Scannell DR,Frank AC,Conant GC,Byrne KP,Woolfit M,Wolfe KH

更新日期：2007-05-15 00:00:00

abstract：:Current theoretical models of the commons assert that common-pool resources can only be managed sustainably with clearly defined boundaries around both communities and the resources that they use. In these theoretical models, open access inevitably leads to a tragedy of the commons. However, in many open-access system...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moritz M,Behnke R,Beitl CM,Bliege Bird R,Chiaravalloti RM,Clark JK,Crabtree SA,Downey SS,Hamilton IM,Phang SC,Scholte P,Wilson JA

更新日期：2018-12-18 00:00:00

abstract：:P elements have been cloned and sequenced from Drosophila nebulosa. Their sequences have diverged less than 6% from P elements of Drosophila melanogaster. However D. nebulosa P elements have nucleotide changes that close all four open reading frames found in the D. melanogaster P element. Microinjection experiments sh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lansman RA,Shade RO,Grigliatti TA,Brock HW

更新日期：1987-09-01 00:00:00

abstract：:The chromophore of photoactive yellow protein (PYP) (i.e., 4-hydroxycinnamic acid) has been replaced by an analogue with a triple bond, rather than a double bond (by using 4-hydroxyphenylpropiolic acid in the reconstitution, yielding hybrid I) and by a "locked" chromophore (through reconstitution with 7-hydroxycoumari...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cordfunke R,Kort R,Pierik A,Gobets B,Koomen GJ,Verhoeven JW,Hellingwerf KJ

更新日期：1998-06-23 00:00:00

abstract：:Natural resistance-associated macrophage protein (Nramp) family transporters catalyze uptake of essential divalent transition metals like iron and manganese. To discriminate against abundant competitors, the Nramp metal-binding site should favor softer transition metals, which interact either covalently or ionically w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bozzi AT,Bane LB,Weihofen WA,McCabe AL,Singharoy A,Chipot CJ,Schulten K,Gaudet R

更新日期：2016-09-13 00:00:00

abstract：:It is generally accepted that adult human bone marrow-derived mesenchymal stromal cells (hMSCs) are default committed toward osteogenesis. Even when induced to chondrogenesis, hMSCs typically form hypertrophic cartilage that undergoes endochondral ossification. Because embryonic mesenchyme is obviously competent to ge...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Occhetta P,Pigeot S,Rasponi M,Dasen B,Mehrkens A,Ullrich T,Kramer I,Guth-Gundel S,Barbero A,Martin I

更新日期：2018-05-01 00:00:00

abstract：:Type II platelet-activating factor-acetylhydrolase [PAF-AH (II)] is an N-myristoylated enzyme that contains a lipase/esterase catalytic motif and selectively hydrolyzes the sn-2 acetyl ester of PAF and other short-chain acyl groups attached to phosphoglycerides. However, the physiological role of this enzyme remains t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Inoue T,Sugimoto A,Suzuki Y,Yamamoto M,Tsujimoto M,Inoue K,Aoki J,Arai H

更新日期：2004-09-07 00:00:00

abstract：:Across phyla, the ribosomes-the central molecular machines for translation of genetic information-exhibit an overall preserved architecture and a conserved functional core. The natural heterogeneity of the ribosome periodically phases a debate on their functional specialization and the tissue-specific variations of th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Amirbeigiarab S,Kiani P,Velazquez Sanchez A,Krisp C,Kazantsev A,Fester L,Schlüter H,Ignatova Z

更新日期：2019-11-05 00:00:00

abstract：:Long-term plasticity can differ from short-term in recruiting the growth of new synaptic connections, a process that requires the participation of both the presynaptic and postsynaptic components of the synapse. How does information about synaptic plasticity spread from its site of origin to recruit the other componen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jin I,Puthanveettil S,Udo H,Karl K,Kandel ER,Hawkins RD

更新日期：2012-06-05 00:00:00

abstract：:The phase transition for turbulent diffusion, reported by Avellaneda and Majda [Avellaneda, M. & Majda, A. J. (1994) Philos. Trans. R. Soc. London A 346, 205-233, and several earlier papers], is traced to a modeling assumption in which the energy spectrum of the turbulent fluid is singularly dependent on the viscosity...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wallstrom TC

更新日期：1995-11-21 00:00:00

abstract：:The mammary gland consists of an adipose tissue that, in a process called branching morphogenesis, is invaded by a ductal epithelial network comprising basal and luminal epithelial cells. Stem and progenitor cells drive mammary growth, and their proliferation is regulated by multiple extracellular cues. One of the key...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kessenbrock K,Smith P,Steenbeek SC,Pervolarakis N,Kumar R,Minami Y,Goga A,Hinck L,Werb Z

更新日期：2017-03-21 00:00:00

abstract：:Although in decline after successful anti-HIV therapy, B-cell lymphomas are still elevated in HIV-1-seropositive (HIV+) persons, and the mechanisms are obscure. The HIV-1 matrix protein p17 persists in germinal centers long after HIV-1 drug suppression, and some p17 variants (vp17s) activate Akt signaling and promote ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dolcetti R,Giagulli C,He W,Selleri M,Caccuri F,Eyzaguirre LM,Mazzuca P,Corbellini S,Campilongo F,Marsico S,Giombini E,Muraro E,Rozera G,De Paoli P,Carbone A,Capobianchi MR,Ippolito G,Fiorentini S,Blattner WA,Lu W,

更新日期：2015-11-17 00:00:00