Orientation of loci within the human major histocompatibility complex by chromosomal in situ hybridization.

abstract：:During neurogenesis of the mammalian neocortex, neural progenitor cells divide to generate daughter cells that either become neurons or remain as progenitor cells. The mouse numb (m-numb) gene encodes a membrane-associated protein that is asymmetrically localized to the apical cell membrane of dividing cortical progen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhong W,Jiang MM,Schonemann MD,Meneses JJ,Pedersen RA,Jan LY,Jan YN

更新日期：2000-06-06 00:00:00

abstract：:Although soft devices (grippers, actuators, and elementary robots) are rapidly becoming an integral part of the broad field of robotics, autonomy for completely soft devices has only begun to be developed. Adaptation of conventional systems of control to soft devices requires hard valves and electronic controls. This ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Preston DJ,Rothemund P,Jiang HJ,Nemitz MP,Rawson J,Suo Z,Whitesides GM

更新日期：2019-04-16 00:00:00

abstract：:Liddle syndrome is a mendelian form of hypertension characterized by constitutively elevated renal Na reabsorption that can result from activating mutations in the beta or gamma subunit of the epithelial Na channel. All reported mutations have deleted the last 45-76 normal amino acids from the cytoplasmic C terminus o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hansson JH,Schild L,Lu Y,Wilson TA,Gautschi I,Shimkets R,Nelson-Williams C,Rossier BC,Lifton RP

更新日期：1995-12-05 00:00:00

abstract：:Understanding interactions between membranes requires measurements on well-controlled systems close to natural conditions, in which fluctuations play an important role. We have determined, by grazing incidence X-ray scattering, the interaction potential between two lipid bilayers, one adsorbed on a solid surface and t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hemmerle A,Malaquin L,Charitat T,Lecuyer S,Fragneto G,Daillant J

更新日期：2012-12-04 00:00:00

abstract：:Tropomyosin is a key component of the contractile systems found in muscle and nonmuscle cells of higher eukaryotes. Based on properties common to all tropomyosins, we have purified a protein from Saccharomyces cerevisiae that resembles tropomyosins from higher cells. The yeast protein remains soluble after heat treatm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu HP,Bretscher A

更新日期：1989-01-01 00:00:00

abstract：:The bacterial pathogen Vibrio parahaemolyticus utilizes a type III secretion system to cause death of host cells within hours of infection. We report that cell death is completely independent of apoptosis and occurs by a mechanism in which injection of multiple type III effectors causes induction of autophagy, cell ro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Burdette DL,Yarbrough ML,Orvedahl A,Gilpin CJ,Orth K

更新日期：2008-08-26 00:00:00

abstract：:Marine ecosystems are sensitive to stochastic environmental variability, with higher-amplitude, lower-frequency--i.e., "redder"--variability posing a greater threat of triggering large ecosystem changes. Here we show that fluctuations in the Pacific Decadal Oscillation (PDO) index have slowed down markedly over the ob...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boulton CA,Lenton TM

更新日期：2015-09-15 00:00:00

abstract：:Chromosomes condense during mitotic entry to facilitate their segregation. Condensation is typically assayed in fixed preparations, limiting analysis of contributing factors. Here, we describe a quantitative method to monitor condensation kinetics in living cells expressing GFP fused to a core histone. We demonstrate ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maddox PS,Portier N,Desai A,Oegema K

更新日期：2006-10-10 00:00:00

abstract：:Elongin is a heterotrimeric transcription elongation factor composed of subunits A, B, and C in mammals. Elongin A and C are F-box-containing and SKP1 homologue proteins, respectively, and are therefore of interest for their potential roles in cell cycle-dependent proteolysis. Mammalian elongin C interacts with both e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Botuyan MV,Koth CM,Mer G,Chakrabartty A,Conaway JW,Conaway RC,Edwards AM,Arrowsmith CH,Chazin WJ

更新日期：1999-08-03 00:00:00

abstract：:Bathorhodopsin-rhodopsin difference spectra of native 11-cis-rhodopsin and regenerated 9-cis-rhodopsin were measured at room temperature with a double-beam laser spectrophotometer after excitation at 532 nm. A detailed analysis of data obtained at 85 psec after excitation suggests that the bathorhodopsins generated fr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spalink JD,Reynolds AH,Rentzepis PM,Sperling W,Applebury ML

更新日期：1983-04-01 00:00:00

abstract：:Dominant mutations in the maize homeobox gene knotted1 (kn1) act nonautonomously during maize leaf development, indicating that Kn1 is involved in the generation or transmission of a developmental signal that passes from the inner layers of the leaf to epidermal cells. We previously found that this nonautonomous activ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim JY,Yuan Z,Cilia M,Khalfan-Jagani Z,Jackson D

更新日期：2002-03-19 00:00:00

abstract：:We describe here the 1.7-A resolution structure of a single-chain antibody variable domain (scFv) molecule, based on the carbohydrate-binding antibody Se155-4, complexed with the trisaccharide ligand alpha-D-Gal(1-->2)[alpha-D-Abe(1-->3)]alpha-D-Manp1-->OMe, where Abe is abequose. The scFv expressed in Escherichia col...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zdanov A,Li Y,Bundle DR,Deng SJ,MacKenzie CR,Narang SA,Young NM,Cygler M

更新日期：1994-07-05 00:00:00

abstract：:Cysteine site-directed mutagenesis was used to create variants of Escherichia coli ribosomal protein L7/L12 that have single cysteine substitutions, at residues 63 or 89, located in different exposed loops in the structure of the globular C-terminal domain indicated by the crystallographic structure. That structure sh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oleinikov AV,Jokhadze GG,Traut RR

更新日期：1993-11-01 00:00:00

abstract：:Here we show how a simple inorganic salt can spontaneously form autocatalytic sets of replicating inorganic molecules that work via molecular recognition based on the {PMo12} ≡ [PMo12O40]3- Keggin ion, and {Mo36} ≡ [H3Mo57M6(NO)6O183(H2O)18]22- cluster. These small clusters are able to catalyze their own formation via...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miras HN,Mathis C,Xuan W,Long DL,Pow R,Cronin L

更新日期：2020-05-19 00:00:00

abstract：:A change-point detection is proposed by using a Bayesian-type statistic based on the shortest Hamiltonian path, and the change-point is estimated by using ratio cut. A permutation procedure is applied to approximate the significance of Bayesian-type statistics. The change-point test is proven to be consistent, and an ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shi X,Wu Y,Rao CR

更新日期：2017-04-11 00:00:00

abstract：:The developmental loss of neurons in sympathetic, sensory, and some parasympathetic ganglia in familial dysautonomia suggests an inherited defect in the action of beta-nerve growth factor (beta-NGF). The role of this growth factor in dysautonomia has been difficult to resolve as there is no known source of authentic h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Breakefield XO,Orloff G,Castiglione C,Coussens L,Axelrod FB,Ullrich A

更新日期：1984-07-01 00:00:00

abstract：:Activation of G protein-coupled receptors (GPCRs) is triggered and regulated by structural rearrangement of the transmembrane heptahelical bundle containing a number of highly conserved residues. In rhodopsin, a prototypical GPCR, the helical bundle accommodates an intrinsic inverse-agonist 11-cis-retinal, which under...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Okada T,Fujiyoshi Y,Silow M,Navarro J,Landau EM,Shichida Y

更新日期：2002-04-30 00:00:00

abstract：:The tumor promoter phorbol 12-myristate 13-acetate (PMA) induces characteristic reversible changes of cell shape in certain fibroblastic lines: motile lamellas are transformed into noncontractile narrow processes; simultaneously, the actin microfilament network of lamellas is locally disorganized. This reaction to PMA...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bershadsky AD,Ivanova OY,Lyass LA,Pletyushkina OY,Vasiliev JM,Gelfand IM

更新日期：1990-03-01 00:00:00

abstract：:In a field study, we show that a young song sparrow (i) selects his songs from three or four older birds who have neighboring territories, (ii) preferentially learns song types that these tutor neighbors share, and (iii) ultimately sets up his territory next to, or replaces, one of these tutor neighbors. The consequen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Beecher MD,Campbell SE,Stoddard PK

更新日期：1994-02-15 00:00:00

abstract：:Interleukin 2 (IL-2)-deficient (IL-2-/-) mice develop hemolytic anemia and chronic inflammatory bowel disease. Importantly, the induction of disease in IL-2-deficient mice is critically dependent on CD4+ T cells. We have studied the requirements of T cells from IL-2-deficient mice for costimulation with B7 antigens. S...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Razi-Wolf Z,Höllander GA,Reiser H

更新日期：1996-04-02 00:00:00

abstract：:The SOS-inducible ruvA and ruvB gene products of Escherichia coli are required for normal levels of genetic recombination and DNA repair. In vitro, RuvA protein interacts specifically with Holliday junctions and, together with RuvB (an ATPase), promotes their movement along DNA. This process, known as branch migration...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsaneva IR,Müller B,West SC

更新日期：1993-02-15 00:00:00

abstract：:Aire induces ectopic expression of peripheral tissue antigens (PTAs) in thymic medullary epithelial cells, which promotes immunological tolerance. Beginning with a broad screen of histone peptides, we demonstrate that the mechanism by which this single factor controls the transcription of thousands of genes involves r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Koh AS,Kuo AJ,Park SY,Cheung P,Abramson J,Bua D,Carney D,Shoelson SE,Gozani O,Kingston RE,Benoist C,Mathis D

更新日期：2008-10-14 00:00:00

abstract：:We have recently purified calorigen, the natural trigger for heat production in the inflorescences of Sauromatum guttatum Schott (voodoo lily), a thermogenic plant, and identified it as salicylic acid. Since then an analytical assay was developed that allows the quantitation of salicylic acid in plant tissues. This as...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Raskin I,Turner IM,Melander WR

更新日期：1989-04-01 00:00:00

abstract：:The Cdc6 protein is essential for the assembly of pre-replicative complexes (pre-RCs) at origins of DNA replication in the budding yeast Saccharomyces cerevisiae. This reaction is blocked in vivo by the cyclin-dependent kinase Cdc28p, together with its regulatory subunits, the B type cyclins that are present throughou...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Donovan S,Harwood J,Drury LS,Diffley JF

更新日期：1997-05-27 00:00:00

abstract：:We describe a method for predicting the structure of alpha beta class proteins in the absence of information from homologous structures. The method is based on an associative memory model for short to intermediate range in sequence contacts and a contact potential for long range in sequence contacts. The coefficients ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hardin C,Eastwood MP,Prentiss MC,Luthey-Schulten Z,Wolynes PG

更新日期：2003-02-18 00:00:00

abstract：:cDNA clones for the (S)-tetrahydroberberine (H4Ber) oxidase of cultured berberine-producing Coptis japonica cells were isolated by screening a C. japonica cDNA library with synthetic nucleotides that can encode the NH2-terminal sequence of this enzyme. Analyses of the nucleotide sequences of the cloned cDNA inserts re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Okada N,Koizumi N,Tanaka T,Ohkubo H,Nakanishi S,Yamada Y

更新日期：1989-01-01 00:00:00

abstract：:RS67333 is a partial serotonin subtype 4 receptor (5-HT4R) agonist that has been widely studied for its procognitive effect. More recently, it has been shown that its ability to promote the nonamyloidogenic cleavage of the precursor of the neurotoxic amyloid-β peptide leads to the secretion of the neurotrophic protein...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lecoutey C,Hedou D,Freret T,Giannoni P,Gaven F,Since M,Bouet V,Ballandonne C,Corvaisier S,Malzert Fréon A,Mignani S,Cresteil T,Boulouard M,Claeysen S,Rochais C,Dallemagne P

更新日期：2014-09-09 00:00:00

abstract：:Diarthrodial joints are essential for load bearing and locomotion. Physiologically, articular cartilage sustains millions of cycles of mechanical loading. Chondrocytes, the cells in cartilage, regulate their metabolic activities in response to mechanical loading. Pathological mechanical stress can lead to maladaptive ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee W,Leddy HA,Chen Y,Lee SH,Zelenski NA,McNulty AL,Wu J,Beicker KN,Coles J,Zauscher S,Grandl J,Sachs F,Guilak F,Liedtke WB

更新日期：2014-11-25 00:00:00

abstract：:Tumor growth requires neoangiogenesis. VEGF is the most potent proangiogenic factor. Dysregulation of hypoxia-inducible factor (HIF) or cytokine stimuli such as those involving the chemokine receptor 4/stromal-derived cell factor 1 (CXCR4/SDF-1) axis are the major cause of ectopic overexpression of VEGF in tumors. Alt...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： de Nigris F,Crudele V,Giovane A,Casamassimi A,Giordano A,Garban HJ,Cacciatore F,Pentimalli F,Marquez-Garban DC,Petrillo A,Cito L,Sommese L,Fiore A,Petrillo M,Siani A,Barbieri A,Arra C,Rengo F,Hayashi T,Al-Omran M,

更新日期：2010-08-10 00:00:00

abstract：:We have used in vitro selection techniques to characterize DNA sequences that are ligated efficiently by T4 RNA ligase. We find that the ensemble of selected sequences ligated about 10 times as efficiently as the random mixture of sequences used as the input for selection. Surprisingly, the majority of the selected se...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Harada K,Orgel LE

更新日期：1993-02-15 00:00:00