Abstract:
:A method to isolate taste buds from the foliate papillae of the rabbit tongue is described. The method comprises (a) separation of the epidermis from the dermal layer after treatment with dilute acetic acid, and (b) mechanical removal of the taste buds from the epithelium with the use of a surgical needle. The procedure yields taste buds that are morphologically well preserved, and in quantities sufficient to enable a detailed biochemical characterization. Preliminary tests have shown the taste buds to have biochemical properties clearly distinct from those of the adjacent epithelium. The method may provide a basis for studying the molecular mechanism of taste perception in greater detail.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Albrecht J,Brouwer JN,Wiersma Adoi
10.1007/BF00229216subject
Has Abstractpub_date
1984-01-01 00:00:00pages
187-9issue
1eissn
0302-766Xissn
1432-0878journal_volume
237pub_type
杂志文章abstract::In the present study, immunogold labeling of ultrathin sections of rat small intestine and liver has been used to obtain insights into the ultrastructural localization and possible functions of annexins. In enterocytes, annexins II, IV, and VI are found at the periphery of the core of each microvillus and of the rootl...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00414181
更新日期:1994-11-01 00:00:00
abstract::The ontogeny of protein gene product 9.5 (PGP 9.5), serotonin (5-HT), calcitonin gene-related peptide (CGRP), and calcitonin (CT) immunoreactivity was evaluated in small-granule endocrine cells of hamster laryngotracheal epithelium from fetal day 11 to adulthood. Two centrifugal (proximal-to-distal) patterns of differ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00305382
更新日期:1994-01-01 00:00:00
abstract::The high affinity IgE receptor on mast cells and basophils is composed of 3 subunits, alpha, beta and gamma. A polyclonal antibody was raised in rabbits to a 13 amino acid synthetic peptide corresponding to the amino terminal portion of the gamma subunit. The antiserum was screened using Western blots of solubilized R...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050575
更新日期:1996-04-01 00:00:00
abstract::Two types of horizontal cells, H1 and H2, were classified using the Golgi technique on flat mounts of the retina of the frog. Cell type H1 possesses rather short, thick dendrites; the diameter of its dendritic field ranges from 40-90 micrometers. H2-cell displays long and thin dendrites; the diameter of its dendritic ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00216747
更新日期:1981-01-01 00:00:00
abstract::The central fat body of the ovoviviparous cockroach Nauphoeta cinerea was studied during the first reproductive cycle of the female by means of light microscopy, autoradiography and electron microscopy. Comparative studies in larval stages were also undertaken. The fat body of Nauphoeta contains a large amount of lipi...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219785
更新日期:1978-04-28 00:00:00
abstract::Cholinergic neurons have been revealed in the enteric nervous system by functional and biochemical studies but not by antibodies that provide excellent localisation of the synthesising enzyme, choline acetyltransferase (ChAT), in the central nervous system. In order to determine whether a newly described peripheral fo...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-002-0652-6
更新日期:2003-01-01 00:00:00
abstract::Intracellular insulin-binding sites were directly traced in fixed monolayer cultures of a variety of cell types with the use of two fluorescent derivatives of insulin, viz. fluorescein isothiocyanate (FITC)-labelled and tetramethyl rhodamine isothiocyanate (TMRITC)-labelled insulin. Both derivatives retained the prope...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00232150
更新日期:1980-01-01 00:00:00
abstract::Mlh1 is a member of DNA mismatch repair (MMR) machinery and is also essential for the stabilization of crossovers during the first meiotic division. Recently, we have shown that zebrafish mlh1 mutant males are completely infertile because of a block in metaphase I, whereas females are fertile but have aneuploid progen...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-007-0550-z
更新日期:2008-04-01 00:00:00
abstract::Long-term in vitro culture (16 days) of caprine ovarian cortical tissue was performed to test the effect of FSH and IGF-I on the viability and development of preantral follicles and mRNA expression for FSH and IGF-I receptors. Fragments were cultured in α-MEM(+) alone or supplemented with different combinations of FSH...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-012-1498-1
更新日期:2012-12-01 00:00:00
abstract::The periodontal ligament (PDL) is a highly specialized tissue connecting the cementum with the tooth socket bone and affects the life span of the tooth. However, little is known about the precise characteristics and regenerative mechanism of PDL cells because of the absence of specific markers and cell lines. Therefor...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-005-0101-4
更新日期:2006-04-01 00:00:00
abstract::The ultrastructural localisation of acid phosphatases (AcPhs) during the normal daily breakdown of rhabdomere membrane in Dinopis has been examined using beta-glycerophosphate and p-nitrophenyl phosphate as substrates. Results are related to the classification of organelles in the receptors given by Blest, Powell and ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00236082
更新日期:1979-07-17 00:00:00
abstract::S-antigen-immunoreactive pinealocytes located in the deep portion of the pineal organ of inbred and wild pigmented mice give rise to long, beaded processes penetrating into the habenular and pretectal regions. In addition, the medial habenular nuclei and the pretectal area contain S-antigen-immunoreactive perikarya, w...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00313528
更新日期:1990-09-01 00:00:00
abstract::Unfertilized eggs commonly lack centrioles, which are usually provided by the male gamete at fertilization, and are unable to assemble functional reproducing centrosomes. However, some insect species lay eggs that develop to adulthood without a contribution from sperm. We report that the oocyte of the parthenogenetic ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-006-0253-x
更新日期:2006-12-01 00:00:00
abstract::Nerve fibers connecting the brain with the pineal gland of the Mongolian gerbil (central pinealopetal fibers) were investigated by means of light and electron microscopy. Several myelinated fibers penetrate from the brain into the deep pineal gland, extend further into the pineal stalk and continue to the superficial ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00213804
更新日期:1983-01-01 00:00:00
abstract::The effects, on the maternal mammary gland, of diets containing similar lipid percentages but differing in composition of polyunsaturated fatty acids (PUFA) have been assessed in rats during pregnancy and lactation. For this purpose, tuna fish oil (an n-3-PUFA-enriched oil) and corn oil (an n-6-PUFA-enriched oil) were...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-012-1523-4
更新日期:2013-03-01 00:00:00
abstract::The fine structure of luminal surface of clearly identified portions of uriniferous tubules has been studied by scanning electron microscopy to elucidate some controversies concerning the topography of certain surface formations. The results show a characteristic pattern of the luminal surface in the region of Henle's...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215128
更新日期:1976-02-06 00:00:00
abstract::Golgi- and fluorescence-histochemical studies in the chicken shown the presence of a sharply delimited group of aminergic neurons beneath the floor of the fourth ventricle at the mesen-metencephalic boundary. According to the observations reported in other avian species a homology can be established between the mammal...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00216221
更新日期:1982-01-01 00:00:00
abstract::To investigate the mechanisms whereby annular gap junctions in the papillary cells of the enamel organ are degraded intracellularly, continuously growing rat incisors were examined by electron microscopy of routine thin sections as well as for the cytochemical localization of inorganic trimetaphosphatase activity. Rou...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215192
更新日期:1986-01-01 00:00:00
abstract::It is hard to fathom that one of the most highly differentiated cells in the body, the spermatozoon, spends over half of its developmental life without the capacity for nuclear protein biosynthesis. This is even more incredible when considering that protein synthesis is switched off long before the sperm is mature. As...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-015-2249-x
更新日期:2016-01-01 00:00:00
abstract::The median eminence/pituitary stalk represents the final common pathway for fibers from neurons that project to the pituitary gland. We have used the lipophilic fluorescent tracer 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) to determine the location of neurons projecting to the median emin...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00304513
更新日期:1995-04-01 00:00:00
abstract::Immunocytochemistry using antibodies against Met-enkephalin and Leu-enkephalin has demonstrated a group of large enkephalin-immunoreactive neurons in the nucleus of the rostral mesencephalic tegmentum (mRMT) of two teleost fish. Salmo gairdneri and Salmo salar. Injections of cobalt-lysine in the medial optic tectum re...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00305731
更新日期:1991-04-01 00:00:00
abstract::The three-dimensional architecture of the lamina propria in the jejunal mucosa of the rat was studied by scanning electron microscopy. The lamina propria is a typical reticular tissue with fine nets of reticular fibers and free and fixed cells lying among them. However, the lamina propria in the core of villi displaye...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00225563
更新日期:1985-01-01 00:00:00
abstract::The fine structure of the horny teeth of the lamprey, Entosphenus japonicus, was examined by light- and electron-microscopy. Most of the horny teeth consisted of two horny and two nonhorny layers. The primary horny layer was well keratinized, and the cells were closely packed and intensely interdigitated, being joined...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215769
更新日期:1983-01-01 00:00:00
abstract::MN9, a monoclonal antibody raised against mouse spermatozoa, specifically recognizes the equatorial segment of sperm head in several mammalian species, including humans. Colloidal gold-immuno-electron microscopy of mouse spermatozoa has shown that the antigen is localized in the space between the outer and inner acros...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00645047
更新日期:1992-12-01 00:00:00
abstract::A pressure device has been used to obtain information about the forces involved in the maintenance of the aggregated state of melanophores of the angelfish, Pterophyllum scalare. Single aggregated melanophores of isolated scales were submitted to mechanical compression with forces ranging from 50-320 mup. As a functio...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219951
更新日期:1975-01-01 00:00:00
abstract::Obesity induces metabolic disorders such as type 2 diabetes, hypertension, and cardiovascular diseases and has become a global health concern. Recent studies imply that fat accumulation in nonadipose tissue correlates with metabolic disorders. However, there are no suitable animal models to evaluate this phenomenon. T...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-018-2908-9
更新日期:2019-02-01 00:00:00
abstract::The globiferous pedicellariae of Psammechinus miliaris are described. Two fixation methods giving minimal distortion and rapid tissue hardening were adapted for soft tissue preparation for scanning electron microscopy. The pedicellarial valves are covered by a microvillous epithelium. The outer valve epithelial microv...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00220184
更新日期:1975-10-13 00:00:00
abstract::This investigation provides histochemical evidence for lysosomal storage of sulfated glycosaminoglycans (GAGs) in the interstitial cells of the renal cortex and in macrophage-like cells of the renal medullary zones of rats chronically treated with the drug tilorone. This compound is known to interfere with lysosomal d...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218958
更新日期:1987-12-01 00:00:00
abstract::Fibrosis is the result of a wound-healing response that fails to restore normal tissue structure function. One of the critical hallmarks of fibrosis is disrupted collagen remodeling. In tissue homeostasis, the production, deposition and organization of collagen is balanced by the degradation and remodeling of collagen...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-016-2440-8
更新日期:2016-09-01 00:00:00
abstract::The smooth muscle of rabbit portal vein was studied by electron microscopy with particular emphasis on the mechanical linkage between the muscle cells and on the distribution of connective tissue. The media of this vein is composed of inner circular and outer longitudinal muscle layers which are orientated almost perp...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00237614
更新日期:1980-01-01 00:00:00