Isolation of taste buds from the foliate papillae of the rabbit.

Abstract:

:A method to isolate taste buds from the foliate papillae of the rabbit tongue is described. The method comprises (a) separation of the epidermis from the dermal layer after treatment with dilute acetic acid, and (b) mechanical removal of the taste buds from the epithelium with the use of a surgical needle. The procedure yields taste buds that are morphologically well preserved, and in quantities sufficient to enable a detailed biochemical characterization. Preliminary tests have shown the taste buds to have biochemical properties clearly distinct from those of the adjacent epithelium. The method may provide a basis for studying the molecular mechanism of taste perception in greater detail.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Albrecht J,Brouwer JN,Wiersma A

doi

10.1007/BF00229216

subject

Has Abstract

pub_date

1984-01-01 00:00:00

pages

187-9

issue

1

eissn

0302-766X

issn

1432-0878

journal_volume

237

pub_type

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