Abstract:
:The effects of Mycoplasma pneumoniae on host cell metabolism were studied by using two types of host cells, MRC-5 human lung fibroblasts, a normal cell line, and Lesch-Nyhan fibroblasts, a cell line deficient in hypoxanthine-guanine phosphoribosyl transferase (EC 2.4.2.8). The susceptibilities of the two cell types were determined by infecting the cells with M. pneumoniae at different multiplicities of infection (MOI). Our data indicate that the Lesch-Nyhan cells were four times more susceptible to damage by M. pneumoniae than the MRC-5 cells. The effects of different MOIs (10 and 50) on de novo purine synthesis. DNA synthesis, and the development of a cytopathic effect were determined. In both cell types, the higher MOI inhibited de novo purine synthesis to a greater extent than the lower MOI. This correlated closely with the cytopathic effect which developed in the monolayers (i.e., the more the inhibition of de novo purine synthesis, the greater the cytopathic effect which developed). In the Lesch-Nyhan cells, DNA synthesis was completely inhibited by the high MOI, whereas in the MRC-5 cells, DNA synthesis was stimulated by the high MOI. In the MRC-5 cells infected with M. pneumoniae, purine salvage activity increased, as indicated by an increase in adenosine deaminase (EC 3.5.4.4) activity. These data indicate that M. pneumoniae alters host cell metabolism, particularly the nucleic acid metabolic pathways. This may explain in part the mechanism of pathogenesis of M. pneumoniae infection.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Upchurch S,Gabridge MGdoi
10.1128/IAI.39.1.164-171.1983subject
Has Abstractpub_date
1983-01-01 00:00:00pages
164-71issue
1eissn
0019-9567issn
1098-5522journal_volume
39pub_type
杂志文章abstract::Pathogenic Leptospira spp. are likely to encounter higher concentrations of reactive oxygen species induced by the host innate immune response. In this study, we characterized Leptospira interrogans catalase (KatE), the only annotated catalase found within pathogenic Leptospira species, by assessing its role in resist...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00466-12
更新日期:2012-11-01 00:00:00
abstract::Cell-free hemoglobin (Hb) is being developed as an erythrocyte substitute. We have previously demonstrated that cell-free Hb is an endotoxin-binding protein which disaggregates endotoxin and subsequently increases the biological activity of endotoxin in several in vitro assays. Because much of the morbidity and mortal...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.4.1258-1266.1997
更新日期:1997-04-01 00:00:00
abstract::Lipopolysaccharide (LPS) binding protein (LBP) is an acute-phase protein that enhances the responsiveness of immune cells to LPS by virtue of its capacity to transfer LPS to CD14. To determine the role of LBP in the innate immune response to peritonitis, LBP gene-deficient (LBP(-/-)) and normal wild-type mice were int...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.12.6747-6753.2003
更新日期:2003-12-01 00:00:00
abstract::Anti-NMDA receptor (NMDAR) autoantibodies have been postulated to play a role in the pathogenesis of NMDAR hypofunction, which contributes to the etiology of psychotic symptoms. Toxoplasma gondii is a pathogen implicated in psychiatric disorders and associated with elevation of NMDAR autoantibodies. However, it remain...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00398-18
更新日期:2018-09-21 00:00:00
abstract::Staphylococcus aureus nasal carriage is a risk factor for infection in humans, particularly in the hospital environment. Attenuation of carriage has proven effective in reducing the prevalence of infection in some high-risk groups. To study staphylococcal factors that influence nasal colonization, a mouse model of S. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.10.5001-5006.1999
更新日期:1999-10-01 00:00:00
abstract::Random-bred mice were immunized with a nonliving antigen prepared from mixed-blood forms of Plasmodium berghei, strain NYU-2, in combination with Corynebacterium parvum and/or living BCG. A high proportion of intravenously immunized mice survived virulent challenge, but subcutaneous vaccination was less effective. Vac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.3.798-803.1978
更新日期:1978-12-01 00:00:00
abstract::Virulent Streptococcus suis serotype 2 strains are invasive extracellular bacteria causing septicemia and meningitis in piglets and humans. One objective of this study was to elucidate the function of complement in innate immune defense against S. suis. Experimental infection of wild-type (WT) and C3(-/-) mice demonst...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00080-14
更新日期:2014-06-01 00:00:00
abstract::The primary antibody response of BALB/c splenocytes to sheep erythrocytes in vitro was suppressed by infection with Friend leukemia virus (FLV), with the response capacity decreasing with increasing duration of infection. The acquisition of normal antibody responses was amplified by macrophage-produced antibody respon...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.3.1260-1264.1983
更新日期:1983-03-01 00:00:00
abstract::Preexisting antivector immunity can severely compromise the ability of Salmonella enterica serovar Typhimurium live vaccines to induce protective CD8 T-cell frequencies after type III secretion system-mediated heterologous protein translocation in orally immunized mice. To circumvent this problem, we injected CpG DNA ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00700-09
更新日期:2009-12-01 00:00:00
abstract::Toxoplasma gondii and Neospora caninum (both Apicomplexa) are closely related cyst-forming coccidian parasites that differ significantly in their host ranges and ability to cause disease. Unlike eutherian mammals, Australian marsupials (metatherian mammals) have long been thought to be highly susceptible to toxoplasmo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00173-17
更新日期:2017-05-23 00:00:00
abstract::The ocular surface is continuously exposed to potential pathogens, including free-living amoebae. Acanthamoeba species are among the most ubiquitous amoebae, yet Acanthamoeba keratitis is remarkably rare. The pathogenesis of Acanthamoeba keratitis is a complex, sequential process. Here we show that Acanthamoeba kerati...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.2.1061-1068.2005
更新日期:2005-02-01 00:00:00
abstract::Human plasma was bactericidal for small numbers of Klebsiella pneumoniae, but larger numbers grew slowly over a period of 24 h. Human polymorphs in a clot of autologous plasma had a bacteriostatic effect on relatively large numbers of bacteria for up to 24 h and were much more effective than plasma alone. The bacteric...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.51.3.723-730.1986
更新日期:1986-03-01 00:00:00
abstract::The adherence and aggregation properties of 46 human oral Streptococcus salivarius isolates were examined. A total of 41% of the isolates aggregated with whole human saliva, 50% aggregated with human erythrocytes, and 85% adhered to human buccal epithelial cells. Strains that aggregated with saliva and erythrocytes us...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
abstract::A canine distemper virus (CDV), DESIGNATED R252, originally recovered from a dog with demyelinating encephalomyelitis was shown to reproduce this disease in gnotobiotic dogs with a high incidence as compared to other CDV strains, which produced an acute fatal infection. In this investigation, R252 was propagated for t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.4.835-844.1975
更新日期:1975-04-01 00:00:00
abstract::Pseudomonas aeruginosa, an important opportunistic pathogen of humans, exploits epithelial damage to establish infection. We have rigorously explored the role of N-glycoproteins and heparan sulfate proteoglycans (HSPGs) in P. aeruginosa-mediated attachment and subsequent downstream events at the apical (AP) and basola...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01215-09
更新日期:2010-03-01 00:00:00
abstract::Although there appears to be little if any role for specific antibodies in protection against intracellular bacteria, such as the model pathogen F. tularensis live vaccine strain (LVS), the role of B cells themselves in primary and secondary infection with such bacteria has not been examined directly. We show here tha...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.11.6002-6007.1999
更新日期:1999-11-01 00:00:00
abstract::The recombinant protective antigen (rPA) of Bacillus anthracis is a promising anthrax vaccine. We compared serum immunoglobulin G levels and toxin-neutralizing antibody titers in rabbits following delivery of various doses of vaccine by microneedle-based intradermal (i.d.) delivery or intramuscular (i.m.) injection us...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01210-06
更新日期:2006-12-01 00:00:00
abstract::Previously, we reported that mutants of Legionella pneumophila lacking a type II secretion (T2S) system elicit higher levels of cytokines (e.g., interleukin-6 [IL-6]) following infection of U937 cells, a human macrophage-like cell line. We now show that this effect of T2S is also manifest upon infection of human THP-1...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00897-16
更新日期:2017-03-23 00:00:00
abstract::The virulence and fitness in vivo of the major human pathogen Staphylococcus aureus are associated with a cell-to-cell signaling mechanism known as quorum sensing (QS). QS coordinates the production of virulence factors via the production and sensing of autoinducing peptide (AIP) signal molecules by the agr locus. Her...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01216-13
更新日期:2014-03-01 00:00:00
abstract::The DNA oligomer 5'-d(TGCGGCCTCTCAGTCCCGCACTTTCATCTTCC)-3' specifically recognizes Haemophilus influenzae 16S rRNA. We report here the use of this oligonucleotide, with a fluorescein label tagged on its 5' end, as a probe for the in situ detection of nonencapsulated nontypeable H. influenzae in sections of adenoid tis...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.2.673-679.1994
更新日期:1994-02-01 00:00:00
abstract::Production of interleukin-10 (IL-10) by C57BL/6 mice following infection with Borrelia burgdorferi has been proposed as a mechanism whereby resistance to the development of experimental Lyme arthritis is maintained. In the current study, we sought to determine the role of IL-10 during infection of arthritis- and cardi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00808-08
更新日期:2008-12-01 00:00:00
abstract::The antiphagocytic effect of the Staphylococcus aureus capsule is known to be related to its ability to interfere with opsonization by normal human serum. In this study, evidence is presented with isolated cell surface components which indicates that the capsule hinders opsonization by masking cell wall peptidoglycan....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.23.2.502-508.1979
更新日期:1979-02-01 00:00:00
abstract::Acquisition of genomic elements by horizontal gene transfer represents an important mechanism in the evolution of bacterial species. Pathogenicity islands are a subset of horizontally acquired elements present in various pathogens. These elements are frequently located adjacent to tRNA genes. We performed a comparativ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.5.2351-2360.2002
更新日期:2002-05-01 00:00:00
abstract::The nucleotide sequences of three previously undescribed Pseudomonas aeruginosa pilin structural genes are presented. Comparisons of deduced pilin primary structure and flanking DNA sequence allowed placement of these and six previously published sequences into one of two groups. Epitope mapping, using overlapping imm...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.2.371-376.1994
更新日期:1994-02-01 00:00:00
abstract::Chlamydia pneumoniae is a human respiratory pathogen. Unlike the other two Chlamydia species, no species-specific antigen has been defined for C. pneumoniae. An immunoreactive clone containing a 0.8-kb fragment was isolated from a C. pneumoniae (AR-39) genomic library by using anti-C. pneumoniae rabbit immune serum. B...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.3.880-886.1994
更新日期:1994-03-01 00:00:00
abstract::The ethanol-insoluble product formed from sucrose by purified enzyme encoded by the gtfA gene from Streptococcus mutans, expressed in recombinant Escherichia coli, was analyzed by 13C and 31P nuclear magnetic resonance. The product was identified as alpha-D-glucose-1-phosphate, and it was concluded that the GTF-A enzy...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.10.2763-2765.1988
更新日期:1988-10-01 00:00:00
abstract::Allergic-type immune responses, particularly immunoglobulin E (IgE), correlate with protective immunity in human schistosomiasis. To better understand the mechanisms of parasite elimination we examined the immune correlates of protection in baboons (Papio cynocephalus anubis), which are natural hosts for Schistosoma m...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.2.636-642.1999
更新日期:1999-02-01 00:00:00
abstract::The salmonid macrophage-like cell line RTS-11 and purified trout pronephros phagocytes were used to analyze in vitro entry and survival of two Streptococcus iniae serotypes. Efficient invasion by S. iniae occurred in both cells, but only the type II strain persisted in pronephros phagocytes for at least 48 h. Ex vivo ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.5.2318-2325.2003
更新日期:2003-05-01 00:00:00
abstract::Bovine sera for tissue culture use were shown to contain heterophil antigen of the Paul-Bunnell-Davidsohn type by immune adherence hemagglutination tests with sera from patients with infectious mononucleosis. The antibody titers observed were comparable to those determined by two other methods; i.e., the immune adhere...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.3.923-927.1978
更新日期:1978-03-01 00:00:00
abstract::An escape variant of Borrelia burgdorferi, selected with a monoclonal antibody to OspB, expressed a truncated form of OspB, the result of point mutations in the ospB gene leading to a premature termination codon. A single amino acid position in the C terminus of OspB was critical for monoclonal antibody recognition. T...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.1.303-307.1994
更新日期:1994-01-01 00:00:00