Mu and delta opiate receptors in rat brain are affected by GTP but not by MIF-1.

Abstract:

:The possible competition of MIF-1 (Pro-Leu-Gly-NH2) with 3H-naloxone or 3H-D-Ala-D-Leu-enkephalin (DADLE) for mu and delta opiate receptors in the rat striatum was examined under conditions of varying temperatures and concentrations of sodium and GTP. MIF-1, in concentrations ranging from 10(-14) to 10(-3) M, failed to compete with either 3H-DADLE or 3H-naloxone for delta or mu receptors, GTP (2 microM) facilitated binding (17.7 +/- 1.9%) at all concentrations of MIF-1 in the 1H-DADLE assay; unexpectedly, GTP facilitated binding in the 3H-naloxone assay (11.5 +/- 1.3%) under conditions used to potentiate antagonist binding (addition of NaCl and incubation at 4 degrees C). GTP showed optimal facilitation at a concentration of about 2 microM in both the 3H-DADLE and 3H-naloxone "antagonist" conditions. At higher doses of GTP, 3H-naloxone binding remained high, but 3H-DADLE binding decreased to near or below control values. MIF-1 did not affect these GTP responses, indicating further the lack of effect of this peptide on opiate binding.

journal_name

Brain Res Bull

journal_title

Brain research bulletin

authors

Luciano MG,Zadina JE,Kastin AJ,Coy DH

doi

10.1016/0361-9230(81)90117-9

subject

Has Abstract

pub_date

1981-12-01 00:00:00

pages

677-82

issue

6

eissn

0361-9230

issn

1873-2747

pii

0361-9230(81)90117-9

journal_volume

7

pub_type

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