Abstract:
:Light-grown, wild type Euglena gracilis contains two aminoacyl-tRNA synthetases for both phenylalanine and isoleucine. Only one of the two synthetases for each amino acid is found in isolated chloroplasts, as are the light-induced phenylalanine and isoleucine tRNAs. In each case the light-induced chloroplast tRNAs can only be acylated by the chloroplast synthetases. The chloroplast isoleucyl-tRNA synthetase is light-inducible and cannot be detected in dark-grown cells or in cells of the bleached mutant W(3)BUL. The presence of the chloroplast phenylalanyl-tRNA synthetase in W(3)BUL, which contains no chloroplast DNA or structure, indicates that this chloroplast enzyme is synthesized in the cytoplasm and is coded by nuclear genes.
journal_name
Proc Natl Acad Sci U S Aauthors
Reger BJ,Fairfield SA,Epler JL,Barnett WEdoi
10.1073/pnas.67.3.1207subject
Has Abstractpub_date
1970-11-01 00:00:00pages
1207-13issue
3eissn
0027-8424issn
1091-6490journal_volume
67pub_type
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