In vitro differentiation of hypothalamic magnocellular neurons of guinea pigs.

Abstract:

:The development of neurophysin-oxytocin and neurophysin-vasopressin containing neurons of the guinea pig was studied in vitro. Supraoptic (SO) and paraventricular (PV) nuclei were explanted from guinea pig foetuses at the 40th day of gestation and cultured in Maximov slides for nearly fifty days. The cultures were observed daily under a phase-contrast microscope. Explants were fixed every five days for observation with the electron microscope. At the time of explantation, magnocellular neurons were still immature. They acquired the morphologic characteristics of mature neurons, with axosomatic synapses, after about 10 days in vitro. After 15-20 days in vitro, they contained in addition neurosecretory granules (NSG), first in the Golgi region, then also dispersed in the cytoplasm. In the oldest culture (45-50 days), signs of granulolysis were regularly found. It appears that magnocellular neurosecretory neurons are able to differentiate in vitro from a primitive state in the absence of specific stimulation.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Marson AM,Privat A

doi

10.1007/BF00233269

subject

Has Abstract

pub_date

1979-01-01 00:00:00

pages

393-401

issue

3

eissn

0302-766X

issn

1432-0878

journal_volume

203

pub_type

杂志文章
  • Pathogenic antibody recognition of cartilage.

    abstract::Antibodies against cartilage proteins are highly prevalent in the sera and synovial fluids of rheumatoid arthritis (RA) patients and also precede disease induction in various spontaneous and induced animal models of arthritis. These antibodies play an important role in the induction and perpetuation of the clinical di...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审

    doi:10.1007/s00441-009-0816-8

    authors: Nandakumar KS

    更新日期:2010-01-01 00:00:00

  • Actin organization and fibrin-clot retractile activity of cultured mouse fibroblasts.

    abstract::It is known that human and animal fibroblasts are able to induce the retraction of a fibrin clot. In the present study the correlation between (i) fibrin-fibroblasts during growth, (ii) the number of actin stress-lines in mouse fibroblasts during growth in culture, and (iii) the sensitivity of actin stress-lines to a ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00218485

    authors: Curatolo L,Chaponnier C,Donati MB,Morasca L,Gabbiani G

    更新日期:1982-01-01 00:00:00

  • Fine structure of the compound eyes and interfacetal mechanoreceptors of Cicindela tranquebarica Herbst (Coleoptera: Cicindelidae).

    abstract::The structure of the compound eyes of adult Cicindela tranquebarica Herbst was examined by use of light, scanning, and transmission electron microscopy. Each ommatidium of these photopic eyes is eucone and has a "subcorneal layer" situated between the corneal lens and crystalline cone. A distal rhabdomere consisting o...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00233613

    authors: Kuster JE

    更新日期:1980-01-01 00:00:00

  • Cell differentiation during early development of Nassarius reticulatus L. (Gastropoda, Prosobranchia). II. Morphological changes of nuclei and nucleoi.

    abstract::In Nassarius reticulatus the nuclei and nucleoli undergo important morphological changes from the zygote to the 16-cell stage. In the zygote and in the trefoil (2-cell) and 4-cell stage, several agranular, fibrillar nucleolus-like bodies 1 mum diameter are present in the interphase nucleus. Granular nucleoli first app...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00215313

    authors: Schmekel L,Fioroni P

    更新日期:1976-05-13 00:00:00

  • Hypertrophy of radial and ulnar arteries following denervation of the chicken wing.

    abstract::Denervation of radial and ulnar arteries in the growing and adult domestic fowl was achieved by unilateral sectioning of the brachial plexus. Eight weeks later the denervated arteries and those of the contralateral wing were examined with light- and electron microscopy to determine the effect of denervation on arteria...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00218955

    authors: Hartley L,Campbell GR

    更新日期:1987-12-01 00:00:00

  • Basic principles and molecular mechanisms of olfactory axon pathfinding.

    abstract::The present review describes several lines of recent evidence providing new insights into the basic principles and mechanisms of axon projection from the olfactory epithelium to the olfactory bulb. Olfactory sensory neurons are classified into approximately 1000 subtypes according to the expression of specific odorant...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审

    doi:10.1007/s004410050953

    authors: Yoshihara Y,Mori K

    更新日期:1997-11-01 00:00:00

  • Immunocytochemical localization of muscarinic acetylcholine receptors in the rat endocrine pancreas.

    abstract::Immunocytochemical application of the antimuscarinic acetylcholine receptor antibody M35 to pancreas tissue revealed the target areas for the parasympathetic nervous system. Immunoreactivity in the endocrine pancreas was much higher than that in the exocrine part. Moreover, the endocrine cells at the periphery of the ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00384730

    authors: Van der Zee EA,Buwalda B,Strubbe JH,Strosberg AD,Luiten PG

    更新日期:1992-07-01 00:00:00

  • Human melanomas express functional P2 X(7) receptors.

    abstract::Adenosine 5'-triphosphate is known to function as a potent extracellular messenger, producing its effects via a distinct family of cell surface receptors. Different receptor subtypes have been shown to modulate different cellular functions such as proliferation, differentiation and apoptosis. We have investigated the ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-005-1149-x

    authors: White N,Butler PE,Burnstock G

    更新日期:2005-09-01 00:00:00

  • Development and organization of polarity-specific segregation of primary vestibular afferent fibers in mice.

    abstract::A striking feature of vestibular hair cells is the polarized arrangement of their stereocilia as the basis for their directional sensitivity. In mammals, each of the vestibular end organs is characterized by a distinct distribution of these polarized cells. We utilized the technique of post-fixation transganglionic ne...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-010-0944-1

    authors: Maklad A,Kamel S,Wong E,Fritzsch B

    更新日期:2010-05-01 00:00:00

  • A freeze-fracture study of perinatal changes of intramembranous particles in microvilli of absorptive cells in mouse small intestine.

    abstract::Perinatal changes in the appearance of intramembranous particles (IMPs) of microvilli of enterocytes were analyzed quantitatively. In both the jejunum and the ileum, the IMP density on the P-face showed no significant changes from day 17 of gestation to day 5 of postnatal life. It increased between day 5 and day 12, r...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00212224

    authors: Arima T,Yamamoto T

    更新日期:1983-01-01 00:00:00

  • Distribution of TRPC1 and TRPC5 in medial temporal lobe structures of mice.

    abstract::The transient receptor potential (TRP) superfamily comprises a group of non-selective cation channels that have been implicated in both receptor and store-operated channel functions. The family of the classical TRPs (TRPCs) consists of seven members (TRPC1-7). The presence of TRPC1 and TRPC5 mRNA in the brain has prev...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-005-0004-4

    authors: von Bohlen Und Halbach O,Hinz U,Unsicker K,Egorov AV

    更新日期:2005-11-01 00:00:00

  • Metastatic growth of squamous cell carcinomas is correlated with upregulation and redistribution of hemidesmosomal components.

    abstract::Bullous pemphigoid antigen-1 (BPA1) and alpha(6)beta(4)-integrin colocalize at the hemidesmosomes in basal-layer keratinocytes of normal squamous epithelia. The expression of these genes was analyzed during the process of tumor cell invasion and metastasis on frozen sections of head and neck biopsies, and the structur...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410100462

    authors: Herold-Mende C,Kartenbeck J,Tomakidi P,Bosch FX

    更新日期:2001-12-01 00:00:00

  • Carbamylcholine-induced morphological changes and spatial dynamics of [Ca2+]c in Harderian glands of guinea pigs: calcium-dependent lipid secretion and contraction of myoepithelial cells.

    abstract::To determine whether lipid-secreting cells have cytosolic Ca2+ concentration ([Ca2+]c)-related secretory mechanisms, morphological changes and intracellular calcium dynamics of Harderian glands of guinea pigs stimulated by secretagogue were studied by electron microscopy and Fura-2/AM digital image analysis. Control g...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00327979

    authors: Satoh Y,Habara Y,Kanno T,Ono K

    更新日期:1993-10-01 00:00:00

  • Mesothelial intercellular junctions and pathways.

    abstract::Freeze-etch preparations of mesothelial cells taken from the peritoneum of mouse reveal the presence of vesicles invaginating the apical and the basal cell surfaces. These vesicles are scarcely seen within the cytoplasm. Long tortuous tubular profiles extend for considerable distance within the cytoplasm and are frequ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00219271

    authors: Baradi AF,Rayns DG

    更新日期:1976-10-01 00:00:00

  • The cercal receptor system of the praying mantid, Archimantis brunneriana Sauss. II. Cercal nerve structure and projection and electrophysiological responses of the individual receptors.

    abstract::The bilaterally paired cercal nerves of Archimantis brunneriana Sauss. leave the terminal ganglion posteriorly and then turn dorsally through muscles at the rear of the abdomen to enter the cerci, where each splits into two branches; successive branchings occur further distally in each cercus. In the distal nerve bran...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00217267

    authors: Ball EE,Boyan GS,Stone RC

    更新日期:1982-01-01 00:00:00

  • Degeneration of osteoblasts involved in intramembranous ossification of fetal rat calvaria.

    abstract::Ossification of calvariae from day-21 rat fetuses was reinvestigated by electron microscopy using different fixation techniques (glutaraldehyde/OsO4, tannic acid, ruthenium red, K-pyroantimonate). An osteoid layer with scattered mineral deposits was found at the mineralization front. Directly beyond this layer, a shee...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00318693

    authors: Zimmermann B

    更新日期:1992-01-01 00:00:00

  • The reversible effect of colchicine on the taste bud cells of the central circumvallate papilla in the rat.

    abstract::It is believed that differentiation and maintenance of taste buds in vertebrates is dependent on the trophic function of their sensory nerve supply. In the present work, colchicine was injected into the circumvallate papilla of the rat. This produced a reversible blockade of neuroplasmic transport and disappearance of...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00231023

    authors: Rodrigo Angulo ML,Fernández Sánchez B,Rodríguez-Echandía EL

    更新日期:1978-08-25 00:00:00

  • A quantitative ultrastructural analysis of changes in hamster cheek-pouch epithelium treated with vitamin A.

    abstract::The ultrastructural changes induced by the topical application of retinol acetate on hamster cheek pouch epithelium were evaluated using stereological analysis. Electron micrographs were prepared of the basal and superficial regions of the nucleated cell layer of the epithelium obtained from 3 treated and 3 control an...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00214783

    authors: Hill MW,Harris RR,Carron CP

    更新日期:1982-01-01 00:00:00

  • Long-term staining of live Merkel cells with FM dyes.

    abstract::Live Merkel cells in the skin and hair follicles are known to incorporate a fluorescence dye, quinacrine, which has been utilized to identify and dissect the cells for experiments. Quinacrine fluorescence of the cells is, however, quickly lost and quinacrine-stained Merkel cells soon become difficult to identify in ti...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-002-0688-7

    authors: Fukuda J,Ishimine H,Masaki Y

    更新日期:2003-03-01 00:00:00

  • Odontoblasts induced from mesenchymal cells of murine dental papillae in three-dimensional cell culture.

    abstract::In an organ culture system under a three-dimensional microenvironment that provides the conditions needed for odontoblast differentiation, a row of odontoblasts can be induced (Kikuchi et al. 1996, 2001). Therefore, in a newly designed three-dimensional cell culture system that fulfils the conditions necessary for odo...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-004-0882-x

    authors: Kikuchi H,Suzuki K,Sakai N,Yamada S

    更新日期:2004-08-01 00:00:00

  • A new type of gap junction in the phylum Brachiopoda.

    abstract::Gap junctions in the phylum Brachiopoda are shown to be of the P type as has been previously reported in invertebrates only in molluscs and possibly bryozoans. However, the mollusc gap junctions appear identical to vertebrae gap junctions in the fracture face on which particles remain, in particle spacing and in parti...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00206345

    authors: Flower NE,Green CR

    更新日期:1982-01-01 00:00:00

  • Metabolic activities of partially degenerated hypertrophic chondrocytes: gene expression of hyaluronan synthases.

    abstract::Ultrastructural aspects of hypertrophic chondrocytes in hamster and mouse epiphysial cartilage were examined in relation to their metabolic activities. With the hypertrophic change, cytoplasmic vacuolization proceeded leaving the partially intact endoplasmic reticulum (ER). In the hypertrophic cells, cytoplasmic hyalu...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004419900082

    authors: Takada Y,Sakiyama H,Kuriiwa K,Masuda R,Inoue N,Nakagawa K,Itano N,Saito T,Yamada T,Kimata K

    更新日期:1999-11-01 00:00:00

  • Studies on the ultrastructure of the rotifer Habrotrocha rosa Donner (Aschelminthes). The alimentary system.

    abstract::The alimentary system of Habrotrocha rosa is composed of the alimentary channel, five digestive glands and another gland which is in close relation to the intestinal syncytium. After the present investigation, the alimentary channel can be divided into sections which clearly differ from each other, these are: oral cav...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00209138

    authors: Schramm U

    更新日期:1978-06-08 00:00:00

  • Rat reduced-folate carrier-1 is localized basolaterally in MDCK kidney epithelial cells and contributes to the secretory transport of methotrexate and fluoresceinated methotrexate.

    abstract::The reduced-folate carrier (Rfc-1), previously also called methotrexate carrier-1 (MTX-1), was recently identified as accounting for approximately 30% of the methotrexate (Mtx) uptake into rat kidney slices. The localization of the carrier and its contribution to secretory or reabsorptive flux of the drug was therefor...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-005-1092-x

    authors: Kneuer C,Honscha KU,Honscha W

    更新日期:2005-06-01 00:00:00

  • Seasonal changes in ultrastructure of brown adipose tissue in the common shrew (Sorex araneus l.).

    abstract::Seasonal changes have been detected in the ultrastructure of brown adipose tissue (BAT) of the common shrew, Sorex araneus (a true nonhibernator) living under natural conditions and collected at the time when the most representative growth phase of the animal for the given season could be expected. In summer and autum...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00233501

    authors: Przełecka A

    更新日期:1981-01-01 00:00:00

  • Distribution and dimension of the T-system in different muscle fiber types in the atlantic hagfish (Myxine glutinosa, L.).

    abstract::Triad density relative to sarcomeres, size of T-system tubules, sarcomere length, muscle fiber diameter in native and fixed states, and size of myofibrils were measured in four straited muscle fiber types in Atlantic hagfishes (Myxine glutinosa, L.) of different sizes. Traids occur at A/I-injunctions in all fiber type...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00223227

    authors: Korneliussen H,Nicolaysen K

    更新日期:1975-01-01 00:00:00

  • Immuno-cytochemical demonstration, in the external region of the amphibian median eminence, of separate vasotocinergic and mesotocinergic nerve fibres.

    abstract::By means of single and double immuno-enzyme cytochemical staining techniques, it was shown that the external region of the amphibian median eminence contains separate vasotocinergic and mesotocinergic nerve fibres. Moreover, it was demonstrated that the vasotocinergic fibres also contain neurophysin. In animals in whi...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00221117

    authors: Dierickx K,Vandesande F

    更新日期:1977-02-02 00:00:00

  • Dominance of parental genomes in embryonic stem cell/fibroblast hybrid cells depends on the ploidy of the somatic partner.

    abstract::Two dozen hybrid clones were produced by fusion of diploid embryonic stem (ES) cells positive for green fluorescent protein (GFP) with tetraploid fibroblasts derived from DD/c and C57BL-I(I)1RK mice. Cytogenetic analysis demonstrated that most cells from these hybrid clones contained near-hexaploid chromosome sets. Ad...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-010-0987-3

    authors: Kruglova AA,Matveeva NM,Gridina MM,Battulin NR,Karpov A,Kiseleva EV,Morozova KN,Serov OL

    更新日期:2010-06-01 00:00:00

  • Phagocytosis in cellular defense and nutrition: a food-centered approach to the evolution of macrophages.

    abstract::The uptake of macromolecules and larger energy-rich particles into the cell is known as phagocytosis. Phagocytosed material is enzymatically degraded in membrane-bound vesicles of the endosome/lysosome system (intracellular digestion). Whereas most, if not all, cells of the animal body are equipped with the molecular ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审

    doi:10.1007/s00441-019-03096-6

    authors: Hartenstein V,Martinez P

    更新日期:2019-09-01 00:00:00

  • Ventricular remodeling of single-chambered myh6-/- adult zebrafish hearts occurs via a hyperplastic response and is accompanied by elastin deposition in the atrium.

    abstract::Zebrafish (Danio rerio) is widely used as an animal model to understand the pathophysiology of cardiovascular diseases. Here, we present the adult cardiac phenotype of weak atrium, myh6-/-, which carry mutations in the zebrafish atrial myosin heavy chain. Homozygous mutants survive to adulthood and are fertile despite...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-019-03044-4

    authors: Sarantis P,Gaitanaki C,Beis D

    更新日期:2019-11-01 00:00:00