Abstract:
:The development of neurophysin-oxytocin and neurophysin-vasopressin containing neurons of the guinea pig was studied in vitro. Supraoptic (SO) and paraventricular (PV) nuclei were explanted from guinea pig foetuses at the 40th day of gestation and cultured in Maximov slides for nearly fifty days. The cultures were observed daily under a phase-contrast microscope. Explants were fixed every five days for observation with the electron microscope. At the time of explantation, magnocellular neurons were still immature. They acquired the morphologic characteristics of mature neurons, with axosomatic synapses, after about 10 days in vitro. After 15-20 days in vitro, they contained in addition neurosecretory granules (NSG), first in the Golgi region, then also dispersed in the cytoplasm. In the oldest culture (45-50 days), signs of granulolysis were regularly found. It appears that magnocellular neurosecretory neurons are able to differentiate in vitro from a primitive state in the absence of specific stimulation.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Marson AM,Privat Adoi
10.1007/BF00233269subject
Has Abstractpub_date
1979-01-01 00:00:00pages
393-401issue
3eissn
0302-766Xissn
1432-0878journal_volume
203pub_type
杂志文章abstract::Antibodies against cartilage proteins are highly prevalent in the sera and synovial fluids of rheumatoid arthritis (RA) patients and also precede disease induction in various spontaneous and induced animal models of arthritis. These antibodies play an important role in the induction and perpetuation of the clinical di...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-009-0816-8
更新日期:2010-01-01 00:00:00
abstract::It is known that human and animal fibroblasts are able to induce the retraction of a fibrin clot. In the present study the correlation between (i) fibrin-fibroblasts during growth, (ii) the number of actin stress-lines in mouse fibroblasts during growth in culture, and (iii) the sensitivity of actin stress-lines to a ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218485
更新日期:1982-01-01 00:00:00
abstract::The structure of the compound eyes of adult Cicindela tranquebarica Herbst was examined by use of light, scanning, and transmission electron microscopy. Each ommatidium of these photopic eyes is eucone and has a "subcorneal layer" situated between the corneal lens and crystalline cone. A distal rhabdomere consisting o...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00233613
更新日期:1980-01-01 00:00:00
abstract::In Nassarius reticulatus the nuclei and nucleoli undergo important morphological changes from the zygote to the 16-cell stage. In the zygote and in the trefoil (2-cell) and 4-cell stage, several agranular, fibrillar nucleolus-like bodies 1 mum diameter are present in the interphase nucleus. Granular nucleoli first app...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215313
更新日期:1976-05-13 00:00:00
abstract::Denervation of radial and ulnar arteries in the growing and adult domestic fowl was achieved by unilateral sectioning of the brachial plexus. Eight weeks later the denervated arteries and those of the contralateral wing were examined with light- and electron microscopy to determine the effect of denervation on arteria...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218955
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journal_title:Cell and tissue research
pub_type: 杂志文章,评审
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更新日期:1997-11-01 00:00:00
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00384730
更新日期:1992-07-01 00:00:00
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-005-1149-x
更新日期:2005-09-01 00:00:00
abstract::A striking feature of vestibular hair cells is the polarized arrangement of their stereocilia as the basis for their directional sensitivity. In mammals, each of the vestibular end organs is characterized by a distinct distribution of these polarized cells. We utilized the technique of post-fixation transganglionic ne...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-010-0944-1
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00212224
更新日期:1983-01-01 00:00:00
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-005-0004-4
更新日期:2005-11-01 00:00:00
abstract::Bullous pemphigoid antigen-1 (BPA1) and alpha(6)beta(4)-integrin colocalize at the hemidesmosomes in basal-layer keratinocytes of normal squamous epithelia. The expression of these genes was analyzed during the process of tumor cell invasion and metastasis on frozen sections of head and neck biopsies, and the structur...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410100462
更新日期:2001-12-01 00:00:00
abstract::To determine whether lipid-secreting cells have cytosolic Ca2+ concentration ([Ca2+]c)-related secretory mechanisms, morphological changes and intracellular calcium dynamics of Harderian glands of guinea pigs stimulated by secretagogue were studied by electron microscopy and Fura-2/AM digital image analysis. Control g...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00327979
更新日期:1993-10-01 00:00:00
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219271
更新日期:1976-10-01 00:00:00
abstract::The bilaterally paired cercal nerves of Archimantis brunneriana Sauss. leave the terminal ganglion posteriorly and then turn dorsally through muscles at the rear of the abdomen to enter the cerci, where each splits into two branches; successive branchings occur further distally in each cercus. In the distal nerve bran...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00217267
更新日期:1982-01-01 00:00:00
abstract::Ossification of calvariae from day-21 rat fetuses was reinvestigated by electron microscopy using different fixation techniques (glutaraldehyde/OsO4, tannic acid, ruthenium red, K-pyroantimonate). An osteoid layer with scattered mineral deposits was found at the mineralization front. Directly beyond this layer, a shee...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00318693
更新日期:1992-01-01 00:00:00
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00231023
更新日期:1978-08-25 00:00:00
abstract::The ultrastructural changes induced by the topical application of retinol acetate on hamster cheek pouch epithelium were evaluated using stereological analysis. Electron micrographs were prepared of the basal and superficial regions of the nucleated cell layer of the epithelium obtained from 3 treated and 3 control an...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00214783
更新日期:1982-01-01 00:00:00
abstract::Live Merkel cells in the skin and hair follicles are known to incorporate a fluorescence dye, quinacrine, which has been utilized to identify and dissect the cells for experiments. Quinacrine fluorescence of the cells is, however, quickly lost and quinacrine-stained Merkel cells soon become difficult to identify in ti...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-002-0688-7
更新日期:2003-03-01 00:00:00
abstract::In an organ culture system under a three-dimensional microenvironment that provides the conditions needed for odontoblast differentiation, a row of odontoblasts can be induced (Kikuchi et al. 1996, 2001). Therefore, in a newly designed three-dimensional cell culture system that fulfils the conditions necessary for odo...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-004-0882-x
更新日期:2004-08-01 00:00:00
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00206345
更新日期:1982-01-01 00:00:00
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journal_title:Cell and tissue research
pub_type: 杂志文章
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更新日期:1999-11-01 00:00:00
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00209138
更新日期:1978-06-08 00:00:00
abstract::The reduced-folate carrier (Rfc-1), previously also called methotrexate carrier-1 (MTX-1), was recently identified as accounting for approximately 30% of the methotrexate (Mtx) uptake into rat kidney slices. The localization of the carrier and its contribution to secretory or reabsorptive flux of the drug was therefor...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-005-1092-x
更新日期:2005-06-01 00:00:00
abstract::Seasonal changes have been detected in the ultrastructure of brown adipose tissue (BAT) of the common shrew, Sorex araneus (a true nonhibernator) living under natural conditions and collected at the time when the most representative growth phase of the animal for the given season could be expected. In summer and autum...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00233501
更新日期:1981-01-01 00:00:00
abstract::Triad density relative to sarcomeres, size of T-system tubules, sarcomere length, muscle fiber diameter in native and fixed states, and size of myofibrils were measured in four straited muscle fiber types in Atlantic hagfishes (Myxine glutinosa, L.) of different sizes. Traids occur at A/I-injunctions in all fiber type...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00223227
更新日期:1975-01-01 00:00:00
abstract::By means of single and double immuno-enzyme cytochemical staining techniques, it was shown that the external region of the amphibian median eminence contains separate vasotocinergic and mesotocinergic nerve fibres. Moreover, it was demonstrated that the vasotocinergic fibres also contain neurophysin. In animals in whi...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00221117
更新日期:1977-02-02 00:00:00
abstract::Two dozen hybrid clones were produced by fusion of diploid embryonic stem (ES) cells positive for green fluorescent protein (GFP) with tetraploid fibroblasts derived from DD/c and C57BL-I(I)1RK mice. Cytogenetic analysis demonstrated that most cells from these hybrid clones contained near-hexaploid chromosome sets. Ad...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-010-0987-3
更新日期:2010-06-01 00:00:00
abstract::The uptake of macromolecules and larger energy-rich particles into the cell is known as phagocytosis. Phagocytosed material is enzymatically degraded in membrane-bound vesicles of the endosome/lysosome system (intracellular digestion). Whereas most, if not all, cells of the animal body are equipped with the molecular ...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-019-03096-6
更新日期:2019-09-01 00:00:00
abstract::Zebrafish (Danio rerio) is widely used as an animal model to understand the pathophysiology of cardiovascular diseases. Here, we present the adult cardiac phenotype of weak atrium, myh6-/-, which carry mutations in the zebrafish atrial myosin heavy chain. Homozygous mutants survive to adulthood and are fertile despite...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-019-03044-4
更新日期:2019-11-01 00:00:00