Abstract:
:The sequence of spontaneous Epstein-Barr virus activation was studied in P3HR-1 carrier cells and in P3HR-1(BrdU) cells made resistant to 5-bromodeoxyuridine. Virus activation was initiated during the normal cell cycle, and recruitment of additional virus-activated cells was prevented by the DNA inhibitors, 1-beta-D-arabinofuranosylcytosine and hydroxyurea. Virus activation was followed by synthesis of the early antigen complex in the absence of additional detectable DNA synthesis. Early antigen synthesis was followed by hydroxyurea-resistant synthesis of new DNA, which in the case of P3HR-1(BrdU) cells was characterized by the appearance of thymidine kinase. The newly synthesized DNA banded in neutral cesium chloride at peaks corresponding to normal human DNA and Epstein-Barr viral DNA. Synthesis of viral antigen was seen only in cells that had undergone hydroxyurea-resistant DNA synthesis.
journal_name
Proc Natl Acad Sci U S Aauthors
Hampar B,Derge JG,Martos LM,Tagamets MA,Burroughs MAdoi
10.1073/pnas.69.9.2589subject
Has Abstractpub_date
1972-09-01 00:00:00pages
2589-93issue
9eissn
0027-8424issn
1091-6490journal_volume
69pub_type
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