Complex network analysis of free-energy landscapes.

abstract：:Chromatin becomes reorganized during mitosis each cell cycle. To identify genes potentially involved in these supramolecular events, we have used a colony-color assay to screen temperature-sensitive mutants of Saccharomyces cerevisiae. When a sequence that mediates attachment to the nuclear matrix in vitro was inserte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fishel BR,Sperry AO,Garrard WT

更新日期：1993-06-15 00:00:00

abstract：:The development of distinct regions in the amniote vertebral column results from somite formation and Hox gene expression, with the adult morphology displaying remarkable variation among lineages. Mammalian regionalization is reportedly very conservative or even constrained, but there has been no study investigating v...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Müller J,Scheyer TM,Head JJ,Barrett PM,Werneburg I,Ericson PG,Pol D,Sánchez-Villagra MR

更新日期：2010-02-02 00:00:00

abstract：:Social status motivates much of human behavior. However, status may have been a relatively weak target of selection for much of human evolution if ancestral foragers tended to be more egalitarian. We test the "egalitarianism hypothesis" that status has a significantly smaller effect on reproductive success (RS) in for...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： von Rueden CR,Jaeggi AV

更新日期：2016-09-27 00:00:00

abstract：:Infections caused by drug-resistant bacteria are a major problem worldwide. Carbapenem-resistant Klebsiella pneumoniae, most notably isolates classified as multilocus sequence type (ST) 258, have emerged as an important cause of hospital deaths. ST258 isolates are predominantly multidrug resistant, and therefore infec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Deleo FR,Chen L,Porcella SF,Martens CA,Kobayashi SD,Porter AR,Chavda KD,Jacobs MR,Mathema B,Olsen RJ,Bonomo RA,Musser JM,Kreiswirth BN

更新日期：2014-04-01 00:00:00

abstract：:We have previously observed the spontaneous, pH-dependent insertion of a water-soluble peptide to form a helix across lipid bilayers [Hunt, J. F., Rath, P., Rothschild, K. J. & Engelman, D. M. (1997) Biochemistry 36, 15177-15192]. We now use a related peptide, pH (low) insertion peptide, to translocate cargo molecules...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reshetnyak YK,Andreev OA,Lehnert U,Engelman DM

更新日期：2006-04-25 00:00:00

abstract：:Antischizophrenic neuroleptic drugs of the diphenylbutylpiperidine class, which includes pimozide, fluspirilene, penfluridol, and clopimozide, inhibit [3H]nitrendipine binding with IC50 values of 13-30 nM. This inhibition involves receptors for the verapamil/prenylamine class of calcium channel antagonists. These diph...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gould RJ,Murphy KM,Reynolds IJ,Snyder SH

更新日期：1983-08-01 00:00:00

abstract：:The effect of phagocytosis on lectin binding to plasma membranes of polymorphonuclear leukocytes was examined. The specific activities of binding sites of concanavalin A and Ricinus communis agglutinin (defined as mug of lectin bound per 100 mug of membrane protein) were measured on isolated membranes; they decreased ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oliver JM,Ukena TE,Berlin RD

更新日期：1974-02-01 00:00:00

abstract：:Conclusive proof that the mouse egg is capable of carrying out repair of genetic lesions present in the male genome was obtained through dominant-lethal studies of chemically treated spermatozoa and spermatids and through cytological analysis of first-cleavage metaphases. The maximum difference in repair capability be...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Generoso WM,Cain KT,Krishna M,Huff SW

更新日期：1979-01-01 00:00:00

abstract：:Isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) represent the two central intermediates in the biosynthesis of isoprenoids. The recently discovered deoxyxylulose 5-phosphate pathway generates a mixture of IPP and DMAPP in its final step by reductive dehydroxylation of 1-hydroxy-2-methyl-2-butenyl 4...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gräwert T,Span I,Eisenreich W,Rohdich F,Eppinger J,Bacher A,Groll M

更新日期：2010-01-19 00:00:00

abstract：:An antiserum was prepared in rabbits against an antigen obtained by density gradient sedimentation of centrifuged medium from monolayer cultures of spleens involved by Hodgkin's disease. The antiserum was tested by isotopic antibody techniques with cells from each of eight cultures derived from spleens involved by Hod...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Long JC,Aisenberg AC,Zamecnik PC

更新日期：1974-07-01 00:00:00

abstract：:The regional chromosomal location of the human gene for plasminogen activator inhibitor type 1 (PAI1) was determined by three independent methods of gene mapping. PAI1 was localized first to 7cen-q32 and then to 7q21.3-q22 by Southern blot hybridization analysis of a panel of human and mouse somatic cell hybrids with ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Klinger KW,Winqvist R,Riccio A,Andreasen PA,Sartorio R,Nielsen LS,Stuart N,Stanislovitis P,Watkins P,Douglas R

更新日期：1987-12-01 00:00:00

abstract：:Magnetic interactions between dissolved oxygen and nitroxide radical spin probes lead to broadening of the ESR lines. We have used a closed-chamber method based on this property to determine the maximum rate of O2 uptake per cell (Vmax per cell) in cultured mammalian cells. A suitable spin probe and a cell suspension ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lai CS,Hopwood LE,Hyde JS,Lukiewicz S

更新日期：1982-02-01 00:00:00

abstract：:Despite the recognized role of tumor necrosis factor (TNF) in inflammation and neuronal degeneration, anti-TNF therapeutics failed to treat neurodegenerative diseases. Animal disease models had revealed the antithetic effects of the two TNF receptors (TNFR) in the central nervous system, whereby TNFR1 has been associa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dong Y,Fischer R,Naudé PJ,Maier O,Nyakas C,Duffey M,Van der Zee EA,Dekens D,Douwenga W,Herrmann A,Guenzi E,Kontermann RE,Pfizenmaier K,Eisel UL

更新日期：2016-10-25 00:00:00

abstract：:Antibody to poly(ADP-ribose) has been covalently coupled to Sepharose and utilized to isolate selectively oligonucleosomes undergoing the poly(ADP-ribosyl)ation reaction from the bulk of chromatin. Approximately 12% of the unfractionated oligonucleosomes were bound to the immunoaffinity column and these represented es...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Malik N,Miwa M,Sugimura T,Thraves P,Smulson M

更新日期：1983-05-01 00:00:00

abstract：:The rat mammary cell line Rama 25 [Bennett, D.C., Peachey, L.A., Durbin, H. & Rudland, P.S. (1978) Cell 15, 283--298] differentiates morphologically in vitro from a cuboidal form to a fusiform cell resembling myoepithelial cells. This differentiation occurs in all clonal isolates of the line. By using three different ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lennon VA,Unger M,Dulbecco R

更新日期：1978-12-01 00:00:00

abstract：:Creatine kinase from rabbit muscle has been specifically labeled with a derivative of a nitroxide free radical on the two essential sulfhydryl groups, one per active site. The conformation in the environment of the enzyme-bound free radical and the change in conformation induced upon binding of the substrate, metal ad...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Taylor JS,Leigh JS Jr,Cohn M

更新日期：1969-09-01 00:00:00

abstract：:Insertion mutations previously constructed within the proximal region of the yeast invertase signal sequence did not interfere with secretion or glycosylation of the enzyme. We now describe deletion mutations within the same signal sequence. Large deletions truncating the hydrophobic core of the signal peptide prevent...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Perlman D,Raney P,Halvorson HO

更新日期：1986-07-01 00:00:00

abstract：:Bacteriophage Mu was inserted into the unc genes of Escherichia coli. The resulting mutation AS12 had a polar effect on the unc operon: membranes of the mutant AS12 contained the dicyclohexylcarbodiimide-binding protein c and the protein a as sole subunits of the ATP synthase. It was shown by peptide mapping and amino...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Friedl P,Bienhaus G,Hoppe J,Schairer HU

更新日期：1981-11-01 00:00:00

abstract：:DNA toroids produced by the condensation of lambda phage DNA with hexammine cobalt (III) have been investigated by cryoelectron microscopy. Image resolution obtained by this technique has allowed unprecedented views of DNA packing within toroidal condensates. Toroids oriented coplanar with the microscope image plane e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hud NV,Downing KH

更新日期：2001-12-18 00:00:00

abstract：:In many plant species only a small proportion of buds yield branches. Both the timing and extent of bud activation are tightly regulated to produce specific branching architectures. For example, the primary shoot apex can inhibit the activation of lateral buds. This process is termed apical dominance and is dependent ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Prusinkiewicz P,Crawford S,Smith RS,Ljung K,Bennett T,Ongaro V,Leyser O

更新日期：2009-10-13 00:00:00

abstract：:The C4B isotype of the fourth component of human complement (C4) displays 3- to 4-fold greater hemolytic activity than does its other isotype C4A. This correlates with differences in their covalent binding efficiencies to erythrocytes coated with antibody and complement C1. C4A binds to a greater extent when C1 is on ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carroll MC,Fathallah DM,Bergamaschini L,Alicot EM,Isenman DE

更新日期：1990-09-01 00:00:00

abstract：:Myosin I, an actin-dependent force-generating enzyme, has been purified from three mammalian sources: bovine adrenal medulla, adrenal cortex, and brain. The purification procedure includes extraction of tissue with ATP at low ionic strength and coprecipitation with actin, followed by gel filtration on Sepharose 4B, an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barylko B,Wagner MC,Reizes O,Albanesi JP

更新日期：1992-01-15 00:00:00

abstract：:Palmitoylation is a common lipid modification known to regulate the functional properties of various proteins and is a vital step in the biosynthesis of voltage-activated sodium (Nav) channels. We discovered a mutation in an intracellular loop of rNav1.2a (G1079C), which results in a higher apparent affinity for exter...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bosmans F,Milescu M,Swartz KJ

更新日期：2011-12-13 00:00:00

abstract：:The pattern of antibody specificities in sera from patients with myasthenia gravis (MG) was determined by the ability of monoclonal antibodies against defined determinants on the acetylcholine receptor molecule to inhibit binding of the serum antibodies to receptor from human muscle. We found that MG patients produce ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tzartos SJ,Seybold ME,Lindstrom JM

更新日期：1982-01-01 00:00:00

abstract：:Differential scanning microcalorimetry provides a noninvasive method for studying heat evolution in living cells. We used this technique to measure the heat evolved by thioglycollate broth-elicited mouse macrophages, and the effects of NaF, KCN, cycloheximide, and cytochalasins B and D on this parameter. The total hea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Loike JD,Silverstein SC,Sturtevant JM

更新日期：1981-10-01 00:00:00

abstract：:We report here the identification of a pollen-specific gene from Zea mays that contains multiple Ser-(Pro)n repeats, the motif found in the cell wall-associated extensins. Sequence analysis reveals that the encoded protein has a putative globular domain at the N terminus and an extensin-like domain at the C terminus. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rubinstein AL,Broadwater AH,Lowrey KB,Bedinger PA

更新日期：1995-04-11 00:00:00

abstract：:We have investigated the mycorrhizal associations of two nonphotosynthetic orchids from distant tribes within the Orchidaceae. The two orchids were found to associate exclusively with two distinct clades of ectomycorrhizal basidiomycetous fungi over wide geographic ranges. Yet both orchids retained the internal mycorr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Taylor DL,Bruns TD

更新日期：1997-04-29 00:00:00

abstract：:A 24-amino acid residue peptide has been isolated from the Fc region of a human IgG1 myeloma protein. The peptide has associated with it the same ability to induce murine B cells to polyclonally secrete antibody as does the intact Fc fragment. Amino acid composition of the peptide indicates that on a mole/mole basis t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morgan EL,Hugli TE,Weigle WO

更新日期：1982-09-01 00:00:00

abstract：:Structure and function of p185neu receptor tyrosine kinase were found to be regulated in a cell cycle-dependent manner. In M phase, p185neu is hyperphosphorylated at serine and/or threonine residues. The phosphotyrosine [Tyr(P)] content of p185neu is at its highest level in G0/G1 phase, decreases through S and G2 phas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kiyokawa N,Yan DH,Brown ME,Hung MC

更新日期：1995-02-14 00:00:00

abstract：:We have investigated whether the large (5- to 15-fold) stimulation of synthesis of growth hormone (somatotropin) observed upon exposure of a strain of rat pituitary (GH3) cells to dexamethasone (a synthetic glucocorticoid) arises from a stimulation of pregrowth hormone mRNA. A good agreement was observed between the s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tushinski RJ,Sussman PM,Yu LY,Bancroft FC

更新日期：1977-06-01 00:00:00