Oligonucleotide-directed mutagenesis screen to identify pathogenic Lynch syndrome-associated MSH2 DNA mismatch repair gene variants.

abstract：:Siphoviridae is the most abundant viral family on earth which infects bacteria as well as archaea. All known siphophages infecting gram+ Lactococcus lactis possess a baseplate at the tip of their tail involved in host recognition and attachment. Here, we report analysis of the p2 phage baseplate structure by X-ray cry...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sciara G,Bebeacua C,Bron P,Tremblay D,Ortiz-Lombardia M,Lichière J,van Heel M,Campanacci V,Moineau S,Cambillau C

更新日期：2010-04-13 00:00:00

abstract：:Decomposition is a critical source of plant nutrients, and drives the largest flux of terrestrial C to the atmosphere. Decomposing soil organic matter typically contains litter from multiple plant species, yet we lack a mechanistic understanding of how species diversity influences decomposition processes. Here, we sho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meier CL,Bowman WD

更新日期：2008-12-16 00:00:00

abstract：:Current chemotherapeutic approaches for cancer are in part limited by the inability of drugs to destroy neoplastic cells within poorly vascularized compartments of tumors. We have here systematically assessed anaerobic bacteria for their capacity to grow expansively within avascular compartments of transplanted tumors...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dang LH,Bettegowda C,Huso DL,Kinzler KW,Vogelstein B

更新日期：2001-12-18 00:00:00

abstract：:The Abeta peptide of Alzheimer disease is derived from the proteolytic processing of the amyloid precursor proteins (APP), which are considered type I transmembrane glycoproteins. Recently, however, soluble forms of full-length APP were also detected in several systems including chromaffin granules. In this report we ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Efthimiopoulos S,Vassilacopoulou D,Ripellino JA,Tezapsidis N,Robakis NK

更新日期：1996-07-23 00:00:00

abstract：:Multisystem Inflammatory Syndrome in Children (MIS-C) associated with COVID-19 is a newly recognized condition in children with recent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. These children and adult patients with severe hyperinflammation present with a constellation of symptoms that st...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheng MH,Zhang S,Porritt RA,Noval Rivas M,Paschold L,Willscher E,Binder M,Arditi M,Bahar I

更新日期：2020-10-13 00:00:00

abstract：:Injection of 3':5'-cyclic AMP (cAMP) into larvae of the fly Sarcophaga bullata 3-4 hr before the beginning of puparium formation (red-spiracle stage) greatly accelerates the onset of tanning without affecting initiation of puparium formation (anterior retraction). Accelerated tanning resembles real tanning in two impo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fraenkel G,Blechl A,Blechl J,Herman P,Seligman MI

更新日期：1977-05-01 00:00:00

abstract：:Mammalian class A macrophage-specific scavenger receptors (SR-A) exhibit unusually broad binding specificity for a wide variety of polyanionic ligands. The properties of these receptors suggest that they may be involved in atherosclerosis and host defense. We have previously observed a similar receptor activity in Dro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pearson A,Lux A,Krieger M

更新日期：1995-04-25 00:00:00

abstract：:Previous studies of legume nodules have indicated that formation of the heme moiety of leghemoglobin is a function of the bacterial symbiont. We now show that a hemA mutant of Bradyrhizobium japonicum that cannot carry out the first step in heme biosynthesis forms fully effective nodules on soybeans. The bacterial mut...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guerinot ML,Chelm BK

更新日期：1986-03-01 00:00:00

abstract：:The involvement of p53 in regulating diverse cellular processes dictates that it must respond to multiple signaling mechanisms, thus coordinating the response to various "stress conditions." Genotoxic stress has served as a paradigm to dissect the transactivation-dependent branch of the pathway by which p53 can induce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ruaro EM,Collavin L,Del Sal G,Haffner R,Oren M,Levine AJ,Schneider C

更新日期：1997-04-29 00:00:00

abstract：:The adzuki bean beetle, Callosobruchus chinensis, is triple-infected with distinct lineages of Wolbachia endosymbiont, wBruCon, wBruOri, and wBruAus, which were identified by their wsp (Wolbachia surface protein) gene sequences. Whereas wBruCon and wBruOri caused cytoplasmic incompatibility of the host insect, wBruAus...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kondo N,Nikoh N,Ijichi N,Shimada M,Fukatsu T

更新日期：2002-10-29 00:00:00

abstract：:Three proteins required for poly(U)-directed polyphenylalanine synthesis have been separated from yeast. Two of the factors correspond to the elongation factors 1 and 2 described for other eukaryotic systems, according to the criteria of phenylalanyl-tRNA binding and diphtheria toxin-catalyzed ADP-ribosylation. The th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Skogerson L,Wakatama E

更新日期：1976-01-01 00:00:00

abstract：:After the start of transcription, the 5' ends of eukaryotic mRNA molecules are modified by the addition of a guanylyl residue to form a cap structure, G(5')ppp(5')N. The guanylyltransferase (GTP:mRNA guanylyltransferase, EC 2.7.7.50) reaction responsible for cap formation usually proceeds via a covalent enzyme-GMP int...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ahola T,Kääriäinen L

更新日期：1995-01-17 00:00:00

abstract：:Strong, short-ranged positional correlations involving counterions can induce a net attractive force between negatively charged strands of DNA and lead to the formation of ion pairs in dilute ionic solutions. However, the long range of the Coulomb interactions impedes the development of a simple local picture. We addr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen YG,Weeks JD

更新日期：2006-05-16 00:00:00

abstract：:A 24-amino acid residue peptide has been isolated from the Fc region of a human IgG1 myeloma protein. The peptide has associated with it the same ability to induce murine B cells to polyclonally secrete antibody as does the intact Fc fragment. Amino acid composition of the peptide indicates that on a mole/mole basis t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morgan EL,Hugli TE,Weigle WO

更新日期：1982-09-01 00:00:00

abstract：:Age-related hearing loss and noise-induced hearing loss are major causes of human morbidity. Here we used genetics and functional studies to show that a shared cause of these disorders may be loss of function of the ATP-gated P2X(2) receptor (ligand-gated ion channel, purinergic receptor 2) that is expressed in sensor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yan D,Zhu Y,Walsh T,Xie D,Yuan H,Sirmaci A,Fujikawa T,Wong AC,Loh TL,Du L,Grati M,Vlajkovic SM,Blanton S,Ryan AF,Chen ZY,Thorne PR,Kachar B,Tekin M,Zhao HB,Housley GD,King MC,Liu XZ

更新日期：2013-02-05 00:00:00

abstract：:A 320-base-pair repeated sequence was observed when DNA samples from the filarial parasites Brugia malayi and Brugia pahangi were digested with the restriction endonuclease Hha I. A 640-base-pair dimer of the repeated sequence from B. malayi was inserted into the plasmid pBR322. When dot hybridization was used, the co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McReynolds LA,DeSimone SM,Williams SA

更新日期：1986-02-01 00:00:00

abstract：:We provide a framework for structural multiscale geometric organization of graphs and subsets of R(n). We use diffusion semigroups to generate multiscale geometries in order to organize and represent complex structures. We show that appropriately selected eigenfunctions or scaling functions of Markov matrices, which d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Coifman RR,Lafon S,Lee AB,Maggioni M,Nadler B,Warner F,Zucker SW

更新日期：2005-05-24 00:00:00

abstract：:The bacteriophage P1 Cre/loxP system has become a powerful tool for in vivo manipulation of the genomes of transgenic mice. Although in vitro studies have shown that Cre can catalyze recombination between cryptic "pseudo-loxP" sites in mammalian genomes, to date there have been no reports of loxP-site infidelity in tr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schmidt EE,Taylor DS,Prigge JR,Barnett S,Capecchi MR

更新日期：2000-12-05 00:00:00

abstract：:A short, highly stereoselective (over 97%) synthesis of all-trans squalene is described. Starting with succinaldehyde, a tetraenedichlorodione having the complete squalene skeleton with the four internal trans olefinic bonds has been developed in four steps involving a sequence of two double Claisen rearrangements. Th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Werthemann L,Johnson WS

更新日期：1970-11-01 00:00:00

abstract：:Fifty cats with feline leukemia virus (FeLV) infection and leukemia-lymphoma complex were treated by ex vivo immunoadsorption with Staphylococcus protein A-bound filters. Most cats responded to therapy. Twelve showed tumor regression, including disappearance of tumor cells, but died later of other complications. Three...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu WT,Engelman RW,Trang LQ,Hau K,Good RA,Day NK

更新日期：1984-06-01 00:00:00

abstract：:It is well known from Hardin's "Tragedy of the Commons" [Hardin G (1968) Science 162:1243-1248] that, if open access is allowed, overgrazing typically results. Hardin, and most authors of the subsequent literature, adopted a static view of the underlying ecosystem. Here we extend this tragedy of the commons to conside...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brekke KA,Øksendal B,Stenseth NC

更新日期：2007-09-11 00:00:00

abstract：:Aortic endothelial cells from normal pigs and pigs with von Willebrand disease have been established in long-term cultures. Both cultures appeared similar in terms of general growth characteristics, morphologic features and ultrastructure. Immunofluorescent staining of these cultures with chicken (or rabbit) antiporci...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Booyse FM,Quarfoot AJ,Bell S,Fass DN,Lewis JC,Mann KG,Bowie EJ

更新日期：1977-12-01 00:00:00

abstract：:To test whether gravity is required for normal amphibian development, Xenopus laevis females were induced to ovulate aboard the orbiting Space Shuttle. Eggs were fertilized in vitro, and although early embryonic stages showed some abnormalities, the embryos were able to regulate and produce nearly normal larvae. These...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Souza KA,Black SD,Wassersug RJ

更新日期：1995-03-14 00:00:00

abstract：:Activated thiol-Sepharose [agarose-(glutathione-2-pyridyl disulfide) conjugate] has been used to immobilize proteins with a single or a few thiol groups via disulfide bridges. The immobilized proteins were subsequently proteolytically degraded. After washing, the thiol-containing peptides were eluted with a reducing a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Egorov TA,Svenson A,Rydén L,Carlsson J

更新日期：1975-08-01 00:00:00

abstract：:Adult-born granule cells in the dentate gyrus of the rodent hippocampus are important for memory formation and mood regulation, but the cellular mechanism underlying their polarized development, a process critical for their incorporation into functional circuits, remains unknown. We found that deletion of the serine-t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang W,She L,Chang XY,Yang RR,Wang L,Ji HB,Jiao JW,Poo MM

更新日期：2014-01-07 00:00:00

abstract：:Transgenic sorghum plants have been obtained after microprojectile bombardment of immature zygotic embryos of a drought-resistant sorghum cultivar, P898012. DNA delivery parameters were optimized based on transient expression of R and C1 maize anthocyanin regulatory elements in scutellar cells. The protocol for obtain...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Casas AM,Kononowicz AK,Zehr UB,Tomes DT,Axtell JD,Butler LG,Bressan RA,Hasegawa PM

更新日期：1993-12-01 00:00:00

abstract：:Triglyceridemic response to a standard oral fat meal was determined in 28 healthy subjects and related to the levels of several lipids, lipoproteins, and apolipoproteins in the post-absorptive plasma. A fatty test meal was administered orally, and postprandial plasma triglyceride levels were determined. In the fasting...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Patsch JR,Karlin JB,Scott LW,Smith LC,Gotto AM Jr

更新日期：1983-03-01 00:00:00

abstract：:Immunoprecipitation of proteins extracted from metabolically labeled human glioblastoma and fibrosarcoma cells with antiserum to platelet-derived growth factor (PDGF) showed that these cells express and secrete proteins that are recognized specifically by the antiserum. The molecular masses of immunoprecipitated prote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pantazis P,Pelicci PG,Dalla-Favera R,Antoniades HN

更新日期：1985-04-01 00:00:00

abstract：:Helicases are molecular motor enzymes that unwind and translocate nucleic acids. One of the central questions regarding helicase activity is whether the process of coupling ATP hydrolysis to DNA unwinding requires an oligomeric form of the enzyme. We have applied a pre-steady-state kinetics approach to address this qu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nanduri B,Byrd AK,Eoff RL,Tackett AJ,Raney KD

更新日期：2002-11-12 00:00:00

abstract：:Motifs of neural circuitry seem surprisingly conserved over different areas of neocortex or of paleocortex, while performing quite different sensory processing tasks. This apparent paradox may be resolved by the fact that seemingly different problems in sensory information processing are related by transformations (ch...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hopfield JJ

更新日期：1996-12-24 00:00:00