Bacterial delta-aminolevulinic acid synthase activity is not essential for leghemoglobin formation in the soybean/Bradyrhizobium japonicum symbiosis.

abstract：:The structural requirements of E. coli formylmethionine tRNA for aminoacylation have been examined by chemical modification of the tRNA, followed by separation of the modified molecules into active and inactive components. Photooxidation of tRNA(fMet) at 50 degrees in the presence of methylene blue results in modifica...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schulman LH

更新日期：1972-12-01 00:00:00

abstract：:The mountain pine beetle (Dendroctonus ponderosae Hopkins) is the most destructive pest of western North American pine forests. Adult males produce frontalin, an eight-carbon antiaggregation pheromone, via the mevalonate pathway, as part of several pheromones that initiate and modulate the mass attack of host trees. F...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Keeling CI,Chiu CC,Aw T,Li M,Henderson H,Tittiger C,Weng HB,Blomquist GJ,Bohlmann J

更新日期：2013-11-19 00:00:00

abstract：:Replication-competent viruses, such as Vaccinia virus (VACV), are powerful tools for the development of oncolytic viral therapies and elicit superior immune responses when used as vaccine and immunotherapeutic vectors. However, severe complications from uncontrolled viral replication can occur, particularly in immunoc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grigg P,Titong A,Jones LA,Yilma TD,Verardi PH

更新日期：2013-09-17 00:00:00

abstract：:Monoclonal antibodies provide an attractive alternative to small-molecule therapies for a wide range of diseases. Given the importance of G protein-coupled receptors (GPCRs) as pharmaceutical targets, there has been an immense interest in developing therapeutic monoclonal antibodies that act on GPCRs. Here we present ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ishchenko A,Wacker D,Kapoor M,Zhang A,Han GW,Basu S,Patel N,Messerschmidt M,Weierstall U,Liu W,Katritch V,Roth BL,Stevens RC,Cherezov V

更新日期：2017-08-01 00:00:00

abstract：:The history of the genus Pan is a topic of enduring interest. Chimpanzees (Pan troglodytes) are often divided into subspecies, but the population structure and genetic history of chimpanzees across Africa remain unclear. Some population genetics studies have led to speculation that, until recently, this species consti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gonder MK,Locatelli S,Ghobrial L,Mitchell MW,Kujawski JT,Lankester FJ,Stewart CB,Tishkoff SA

更新日期：2011-03-22 00:00:00

abstract：:Investigation of retinal neurochemistry in a well-defined chick model of form-deprivation myopia indicated that dopamine and its metabolite 3,4-dihydroxyphenylacetic acid are reduced in myopic as compared to control eyes. The reduction in retinal dopamine is evident only during light adaptation and is accompanied by a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stone RA,Lin T,Laties AM,Iuvone PM

更新日期：1989-01-01 00:00:00

abstract：:Combining genotypes across datasets is central in facilitating advances in genetics. Data aggregation efforts often face the challenge of record matching-the identification of dataset entries that represent the same individual. We show that records can be matched across genotype datasets that have no shared markers ba...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Edge MD,Algee-Hewitt BFB,Pemberton TJ,Li JZ,Rosenberg NA

更新日期：2017-05-30 00:00:00

abstract：:Two molecular forms of pyrophosphate-dependent phosphofructokinase (PP(i)-PFK; pyrophosphate:D-fructose-6-phosphate 1-phosphotransferase; EC 2.7.1.90) have been found whose activity depends upon association and dissociation characteristics regulated by fructose 2,6-bisphosphate (Fru-2,6-P(2)). PP(i)-PFK was purified 2...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu MX,Smyth DA,Black CC

更新日期：1984-08-01 00:00:00

abstract：:Single-stranded DNA oligonucleotides can achieve targeted base-pair substitution with modest efficiency but high precision. We show that "oligo targeting" can be used effectively to study missense mutations in DNA mismatch repair (MMR) genes. Inherited inactivating mutations in DNA MMR genes are causative for the canc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Houlleberghs H,Dekker M,Lantermans H,Kleinendorst R,Dubbink HJ,Hofstra RM,Verhoef S,Te Riele H

更新日期：2016-04-12 00:00:00

abstract：:We posit that feasible reversal of the growth of atmospheric CH(4) and other trace gases would provide a vital contribution toward averting dangerous anthropogenic interference with global climate. Such trace gas reductions may allow stabilization of atmospheric CO(2) at an achievable level of anthropogenic CO(2) emis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hansen J,Sato M

更新日期：2004-11-16 00:00:00

abstract：:We show that a large class of finite factors has free entropy dimension less than or equal to one. This class includes all prime factors and many property T factors. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ge L,Shen J

更新日期：2000-08-29 00:00:00

abstract：:An explicitly polarizable force field based exclusively on quantum data is applied to calculations of relative binding affinities of ligands to proteins. Five ligands, differing by replacement of an atom or functional group, in complexes with three serine proteases-trypsin, thrombin, and urokinase-type plasminogen act...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Khoruzhii O,Donchev AG,Galkin N,Illarionov A,Olevanov M,Ozrin V,Queen C,Tarasov V

更新日期：2008-07-29 00:00:00

abstract：:We noticed that disease-related amyloidogenic proteins and especially cellular prion proteins have the highest proportion of incompletely desolvated backbone H bonds among soluble proteins. Such bonds are vulnerable to water attack and thus represent structural weaknesses. We have measured the adsorption of proteins o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fernández A,Berry RS

更新日期：2003-03-04 00:00:00

abstract：:Domesticated maize and its wild ancestor (teosinte) differ strikingly in morphology and afford an opportunity to examine the connection between strong selection and diversity in a major crop species. The tb1 gene largely controls the increase in apical dominance in maize relative to teosinte, and a region of the tb1 l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Clark RM,Linton E,Messing J,Doebley JF

更新日期：2004-01-20 00:00:00

abstract：:The trilaminate vascular architecture provides biochemical regulation and mechanical integrity. Yet regulatory control can be regained after injury without recapitulating tertiary structure. Tissue-engineered (TE) endothelium controls repair even when placed in the perivascular space of injured vessels. It remains unc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zani BG,Kojima K,Vacanti CA,Edelman ER

更新日期：2008-05-13 00:00:00

abstract：:Heterosis is widely applied in agriculture; however, the underlying molecular mechanisms for superior performance are not well understood. Ethylene biosynthesis and signaling genes are shown to be down-regulated in Arabidopsis interspecific hybrids. Ethylene is a plant hormone that promotes fruit ripening and maturati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Song Q,Ando A,Xu D,Fang L,Zhang T,Huq E,Qiao H,Deng XW,Chen ZJ

更新日期：2018-05-22 00:00:00

abstract：:The ability to dynamically organize functional nanoparticles (NPs) via the use of environmental triggers (temperature, pH, light, or solvent polarity) opens up important perspectives for rapid and convenient construction of a rich variety of complex assemblies and materials with new structures and functionalities. Her...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen Y,Wang Z,He Y,Yoon YJ,Jung J,Zhang G,Lin Z

更新日期：2018-02-13 00:00:00

abstract：:The posteromedial barrel subfield of the somatosensory cortex of mice was examined histochemically for cytochrome oxidase activity (cytochrome c oxidase; ferrocytochrome c:oxygen oxidoreductase, EC 1.9.3.1). In normal mice a high enzymatic activity was found within the barrel hollows, rather than in the sides and sept...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wong-Riley MT,Welt C

更新日期：1980-04-01 00:00:00

abstract：:Climate change is expected to influence the capacities of the land and oceans to act as repositories for anthropogenic CO2 and hence provide a feedback to climate change. A series of experiments with the National Center for Atmospheric Research-Climate System Model 1 coupled carbon-climate model shows that carbon sink...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fung IY,Doney SC,Lindsay K,John J

更新日期：2005-08-09 00:00:00

abstract：:Four monoclonal anti-parathyroid antibodies were generated after immunization of mice with intact cells from human parathyroid tissue. All four monoclonal antibodies reacted in immunohistochemistry with structures present on parathyroid epithelial cells and proximal-tubule cells of the kidney but were unreactive with ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Juhlin C,Holmdahl R,Johansson H,Rastad J,Akerström G,Klareskog L

更新日期：1987-05-01 00:00:00

abstract：:The transforming growth factors beta (TGF-beta s) are important modulators of growth and differentiation. They are intermolecular disulfide-bonded homodimeric molecules. The monomer fold has a conserved cystine knot and lacks a hydrophobic core. The biological specificity of a given member of the family is believed to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Venkataraman G,Sasisekharan V,Cooney CL,Langer R,Sasisekharan R

更新日期：1995-06-06 00:00:00

abstract：:The 1H NMR signal from oxymyoglobin, a low-concentration diamagnetic protein, is visible in myocardial tissue. The methyl group of the Val-E11 resonates in a clear spectral region at -2.76 ppm and responds to dynamic changes in cellular oxygenation. With CO, the signal shifts to -2.4 ppm. The Val-E11 peak assignment a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kreutzer U,Wang DS,Jue T

更新日期：1992-05-15 00:00:00

abstract：:Although recent studies have shown that brown adipose tissue (BAT) arises from progenitor cells that also give rise to skeletal muscle, the developmental signals that control the formation of BAT remain largely unknown. Here, we show that brown preadipocytes possess primary cilia and can respond to Hedgehog (Hh) signa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nosavanh L,Yu DH,Jaehnig EJ,Tong Q,Shen L,Chen MH

更新日期：2015-04-21 00:00:00

abstract：:We identified the phosphatidylinositol transfer protein (PITP) as being responsible for a powerful latent, nucleotide-independent, Golgi-vesiculating activity that is present in the cytosol but is only manifested as an uncontrolled activity in a cytosolic protein subfraction, in which it is separated from regulatory c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Simon JP,Morimoto T,Bankaitis VA,Gottlieb TA,Ivanov IE,Adesnik M,Sabatini DD

更新日期：1998-09-15 00:00:00

abstract：:A special methionine tRNA (tRNAi) is universally required to initiate translation. Amongst species a tRNAi structural conservation is most apparent in the anticodon and T arms of the molecule but extends into the variable loop and the 3' strand of the D stem. This suggested that they could share a similar ancestral or...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ganoza MC,Marliere P,Kofoid EC,Louis BG

更新日期：1985-07-01 00:00:00

abstract：:The signal-transducing guanine nucleotide-binding regulatory (G) proteins are heterotrimers composed of three subunits--alpha, beta, and gamma. Although multiple distinctive forms of the alpha subunit have been described, only two forms of the beta subunits of the G proteins have been identified. To investigate furthe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levine MA,Smallwood PM,Moen PT Jr,Helman LJ,Ahn TG

更新日期：1990-03-01 00:00:00

abstract：:Immunoaffinity-purified interleukin 2 (IL2) stimulated proliferation of large but not small B cells. Stimulation was observed even when B cells were cultured at very low cell densities (3 X 10(4) per microwell containing 0.2 ml of medium). Addition of small numbers of purified splenic T cells did not enhance the IL2-i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mond JJ,Thompson C,Finkelman FD,Farrar J,Schaefer M,Robb RJ

更新日期：1985-03-01 00:00:00

abstract：:Phyllosphere microbial communities were evaluated on leaves of field-grown plant species by culture-dependent and -independent methods. Denaturing gradient gel electrophoresis (DGGE) with 16S rDNA primers generally indicated that microbial community structures were similar on different individuals of the same plant sp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang CH,Crowley DE,Borneman J,Keen NT

更新日期：2001-03-27 00:00:00

abstract：:We have studied the genetic interactions between mutant alleles in 12 genes, designated PER1-PER12, which are essential for peroxisome biogenesis in the yeast Hansenula polymorpha. Recessive mutations in any of these genes determined three different morphological phenotypes: (i) complete absence of peroxisomes (Per-);...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Titorenko VI,Waterham HR,Cregg JM,Harder W,Veenhuis M

更新日期：1993-08-15 00:00:00

abstract：:Major histocompatibility complex (MHC) class II molecules are heterodimeric glycoproteins with one alpha and one beta polypeptide chain of similar molecular size. In this report, we describe the binding of an acetylated N-terminal peptide of myelin basic protein, [Ala4]MBP-(1-14), to purified individual alpha and beta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nag B,Wada HG,Deshpande SV,Passmore D,Kendrick T,Sharma SD,Clark BR,McConnell HM

更新日期：1993-02-15 00:00:00