Magnetic resonance imaging using gadolinium labeled monoclonal antibody.

Abstract:

:Gadolinium was attached to antibodies and tested in vitro and in vivo for its effect on proton relaxation enhancement. Using the cyclic anhydride method, diethylenetriaminepentaacetic acid (DTPA) was attached to albumin, IgG and anti-CEA monoclonal antibody. Gadolinium (Gd) was then chelated to the protein complexes forming protein-DTPA-Gd complex. With this technique approximately 9 atoms of Gd could be attached to each albumin molecule, 4 to each IgG molecule and 1.5 to each monoclonal antibody molecule. The minimal in vitro concentration of Gd in the form of IgG-DTPA-Gd necessary to produce proton relaxation enhancement at 0.35 tesla was 10(-1) mM. An in vivo experiment using anticarcinoembryonic antigen (CEA) monoclonal antibody-DTPA-Gd in hamsters implanted with human colon carcinoma resulted in a tumor concentration of Gd of less than 10(-4) mM. No enhancement of the tumors was detected at that concentration. For monoclonal antibodies to function as selective MR contrast agents, substantial advances in technology must occur.

journal_name

Invest Radiol

journal_title

Investigative radiology

authors

Unger EC,Totty WG,Neufeld DM,Otsuka FL,Murphy WA,Welch MS,Connett JM,Philpott GW

doi

10.1097/00004424-198510000-00008

subject

Has Abstract

pub_date

1985-10-01 00:00:00

pages

693-700

issue

7

eissn

0020-9996

issn

1536-0210

journal_volume

20

pub_type

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