Contrast media-induced chromosomal damage in human lymphocyte cultures.

Abstract:

:Ionic (diatrizoate, ioxaglate) and nonionic (iohexol, iosimide, iopromide, and iotrolan) contrast media (CM) were evaluated for their cytogenetic effects in lymphocytes. Heparinized blood was mixed with culture medium RPMI-1640 supplemented with phytohemagglutinin, fetal calf serum, and antibiotics. Plastic tubes containing blood samples were incubated at 37 degrees C in 5% CO2 humidified air for 48 hours. To these cultures, increasing amounts of CM were added and cells incubated for an additional 24 hours. After this exposure, red blood cells were lysed with hypotonic KC1, lymphocyte smears fixed on glass slides and stained with May-Grünwald Giemsa. Chromosomal damage was analyzed by a micronucleus test. All CM tested induced micronuclei in lymphocytes quite significantly (P less than .001) when compared with the frequency of micronuclei in controls. These observations on the genotoxic potential of nonionic CM suggest that factors other than ionic composition and osmolality are involved in clastogenesis; further studies are needed to establish the molecular mechanisms in CM induced chromosomal damage.

journal_name

Invest Radiol

journal_title

Investigative radiology

authors

Parvez Z,Kormano M,Moncada R,Eklund R

doi

10.1097/00004424-198611000-00006

subject

Has Abstract

pub_date

1986-11-01 00:00:00

pages

864-9

issue

11

eissn

0020-9996

issn

1536-0210

journal_volume

21

pub_type

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