Abstract:
:The identity of each of 239 yeasts, encompassing 9 genera and 30 species, was determined with the Quantum II and API 20C identification systems. With API 20C results accepted as being correct, Quantum II proved to be 92% accurate in identification of common isolates, e.g., Candida albicans and Torulopsis glabrata, but only 73% effective with less frequently encountered yeasts, e.g., Trichosporon beigelii and Rhodotorula glutinis. Overall, Quantum II was 86% as accurate as API 20C for the yeast isolates tested. In addition, physical problems were encountered in inoculation of Quantum II cartridges and in the automated reading of some biochemical tests. We conclude that Quantum II is not yet suitable for routine identification of clinical yeast isolates.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Salkin IF,Schadow KH,Bankaitis LA,McGinnis MR,Kemna MEdoi
10.1128/JCM.22.3.442-444.1985subject
Has Abstractpub_date
1985-09-01 00:00:00pages
442-4issue
3eissn
0095-1137issn
1098-660Xjournal_volume
22pub_type
杂志文章abstract::Diarrheal diseases are highly prevalent in Bangladesh. However, the relative contribution of diarrheagenic Escherichia coli organisms--those that are enterotoxigenic (ETEC), enteropathogenic (EPEC), enteroinvasive, enterohemorrhagic, enteroaggregative, and diffuse adherent--to diarrhea in Bangladeshi populations is no...
journal_title:Journal of clinical microbiology
pub_type: 临床试验,杂志文章
doi:10.1128/JCM.33.4.973-977.1995
更新日期:1995-04-01 00:00:00
abstract::Two types of commercially available microdilution trays were inoculated with log-phase cultures, stationary-phase cultures, and direct suspensions of 18- to 24-h colonies, with essentially comparable results. Direct pick-up of colonies was also tested with two Prompt inoculation systems (3M Co.); they were found to be...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.18.3.645-651.1983
更新日期:1983-09-01 00:00:00
abstract::Staphylococcus aureus ST45 is a major global MRSA lineage with huge strain diversity and a high clinical impact. It is one of the most prevalent carrier lineages but also frequently causes severe invasive disease, such as bacteremia. Little is known about its evolutionary history. In this study, we used whole-genome s...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02198-20
更新日期:2020-12-17 00:00:00
abstract::Moxalactam is highly hydrolyzed by plasmid-mediated metallo-β-lactamases (MBLs), whereas it is poorly inactivated by serine-active carbapenemases. This study demonstrated that moxalactam resistance constituted an effective screen for MBL expression in enterobacteria, which could be confirmed, even in low-MBL-producing...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00328-11
更新日期:2011-07-01 00:00:00
abstract::The impact of the viability of Trichomonas vaginalis in urine on wet mount, culture, and PCR methods was assessed. To minimize the chance of false-negative results, urine specimens should be processed within 30 min of specimen collection and maintained at 37 degrees C, since temperature appears to affect the viability...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01287-06
更新日期:2006-10-01 00:00:00
abstract::Amphotericin B therapy continues to be the "gold standard" in the treatment of invasive aspergillosis in the immunocompromised host. Although Aspergillus fumigatus and Aspergillus flavus constitute the major species, several reports have described invasive pulmonary or disseminated disease due to the less common Asper...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.37.7.2343-2345.1999
更新日期:1999-07-01 00:00:00
abstract::Adolescent children were surveyed for colonization with Mycoplasma hominis and Ureaplasma urealyticum by culturing urine specimens. Rates were compared between three study groups: (i) 397 children attending parochial schools, (ii) 293 children attending an adolescent clinic specializing in adjustment problems, and (ii...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
更新日期:1975-09-01 00:00:00
abstract::Microfiltration has become a popular procedure for the concentration and enumeration of bacteria. We developed a rapid and sensitive method for the differentiation of gram-positive and gram-negative bacteria, utilizing a polycarbonate membrane filter, crystal violet, iodine, 95% ethanol, and 6% carbol fuchsin, that ca...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.26.7.1378-1382.1988
更新日期:1988-07-01 00:00:00
abstract::Coagulase-negative staphylococci obtained from patients with continuous ambulatory peritoneal dialysis-related peritonitis were cultured in nutrient broth in an atmosphere of air containing 5% carbon dioxide (reflecting the CO2 tension found in freshly used dialysate). Significant differences were observed between the...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.28.8.1813-1817.1990
更新日期:1990-08-01 00:00:00
abstract::Multidrug-resistant opportunistic pathogens have become endemic to the veterinary hospital environment. Escherichia coli isolates resistant to 12 antibiotics were isolated from two dogs that were housed in the intensive care unit at The University of Georgia Veterinary Teaching Hospital within 48 h of each other. Revi...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.40.10.3586-3595.2002
更新日期:2002-10-01 00:00:00
abstract::A mycobacterium isolated from a clinical sample of an AIDS patient was identified as Mycobacterium interjectum by direct 16S rRNA sequence determination. High-performance liquid chromatography, however, revealed a mycolic acid pattern which was different from the one shared by the previously analyzed strains of this s...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.34.9.2316-2319.1996
更新日期:1996-09-01 00:00:00
abstract::A 45-year-old man from Nepal with a 13-year history of polycystic kidney disease was diagnosed as suffering from chronic renal failure with end-stage renal disease. After receiving empirical antituberculosis treatment, he was treated with broad-spectrum antibiotics. A left nephrectomy was performed, and after 4 months...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.41.1.479-482.2003
更新日期:2003-01-01 00:00:00
abstract::Streptococcus pneumoniae is both a commensal and a major pathogen that causes invasive disease in people of all ages. The introduction of serotype-specific pneumococcal vaccines has reduced the burden of disease but has also led to replacement with new strains; thus, serotyping remains important for vaccine-related di...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00613-16
更新日期:2016-07-01 00:00:00
abstract::Tuberculous meningitis (TBM) is the most severe form of tuberculosis. Microbiological confirmation is rare, and treatment is often delayed, increasing mortality and morbidity. The GeneXpert MTB/RIF test was evaluated in a large cohort of patients with suspected tuberculous meningitis. Three hundred seventy-nine patien...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01834-13
更新日期:2014-01-01 00:00:00
abstract::Several media were used to evaluate the hemolytic activity of Listeria monocytogenes, and this property was used along with the API Listeria system to identify Listeria spp. All L. monocytogenes strains were identified correctly with this system, and blood agar base no. 2 and Columbia blood agar base supplemented with...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.32.4.1127-1129.1994
更新日期:1994-04-01 00:00:00
abstract::Lyme borreliosis is a tick-borne disease caused by the Borrelia burgdorferisensu lato complex. Bio-Rad Laboratories has developed a fully automated multiplex bead-based assay for the detection of IgM and IgG antibodies to B. burgdorferi The BioPlex 2200 Lyme Total assay exhibits an improved rate of seropositivity in p...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01785-19
更新日期:2020-04-23 00:00:00
abstract::An enzyme-linked immunosorbent assay was used to measure nasal-wash and serum isotype-specific hemagglutinin antibody responses in 109 seronegative (hemagglutination-inhibiting titer less than or equal to 1:8) adults vaccinated intranasally with live attenuated A/Washington/897/80 (H3N2) or A/California/10/78 (H1N1) c...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.23.1.66-72.1986
更新日期:1986-01-01 00:00:00
abstract::Enteroviruses have been reported in encephalitis cases. However, clinical and epidemiological characteristics of enteroviruses in encephalitis are not fully established. We prospectively investigated 204 children with encephalitis over a period of 2 years (2009 to 2010) for enterovirus. Enterovirus was detected in 45 ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01483-12
更新日期:2012-11-01 00:00:00
abstract::A 10-min latex agglutination test kit, Culturette Brand Ten-Minute Group A Strep ID (Marion Scientific, Div. of Marion Laboratories, Inc., Kansas City, Mo.), was assessed for the rapid detection of group A beta-hemolytic streptococcus directly from a throat swab. Four hundred and thirty-five throat swab specimens from...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.21.2.258-259.1985
更新日期:1985-02-01 00:00:00
abstract::Pathozyme-Myco G (Myco G), M, A, and TB complex plus (Omega Diagnostics Ltd., Alloa, Scotland) were evaluated for the serological diagnosis of pulmonary tuberculosis (TB) in an Argentinean population. Sera from 58 patients with pulmonary TB, 24 subjects with pulmonary mycobacteriosis or mycoses (pulmonary MM group), a...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.42.2.884-887.2004
更新日期:2004-02-01 00:00:00
abstract::Nineteen strains of nutritionally variant streptococci (NVS) were tested for bacteriolytic activity and for their production of and sensitivity to streptococcal bacteriocinlike inhibitory substances (BLIS). None appeared to produce BLIS. An inverse relationship was found between the sensitivity to BLIS and the product...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.29.4.848-849.1991
更新日期:1991-04-01 00:00:00
abstract::A radioimmunoassay for determination of human immunoglobulin G, A, and M class antibodies to Histoplasma capsulatum is reported. Yeast-phase antigen from H. capsulatum was adsorbed onto polyvinyl chloride microtiter plates as the solid phase. Human antibodies were allowed to react with this antigen, followed by additi...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.13.1.66-72.1981
更新日期:1981-01-01 00:00:00
abstract::A new DNA probe assay (INNO LiPA Mycobacteria; Innogenetics, Ghent, Belgium) for the simultaneous identification, by means of reverse hybridization and line-probe technology, of Mycobacterium tuberculosis complex, Mycobacterium kansasii, Mycobacterium xenopi, Mycobacterium gordonae, the species of the Mycobacterium av...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.39.3.1079-1084.2001
更新日期:2001-03-01 00:00:00
abstract::The human cytomegalovirus (HCMV) a-sequence (a-seq) is located in the joining region between the long (L) and short (S) unique sequences of the virus (L-S junction), and this hypervariable junction has been used to differentiate HCMV strains. The purpose of this study was to investigate whether there are differences a...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.28.12.2602-2607.1990
更新日期:1990-12-01 00:00:00
abstract::Severe early childhood caries (ECC), while strongly associated with Streptococcus mutans using selective detection (culture, PCR), has also been associated with a widely diverse microbiota using molecular cloning approaches. The aim of this study was to evaluate the microbiota of severe ECC using anaerobic culture. Th...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02427-10
更新日期:2011-04-01 00:00:00
abstract::Nontuberculous mycobacterial infections caused by Mycobacterium abscessus are responsible for a range of disease manifestations from pulmonary to skin infections and are notoriously difficult to treat, due to innate resistance to many antibiotics. Previous population studies of clinical M. abscessus isolates utilized ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01144-14
更新日期:2014-10-01 00:00:00
abstract::Sera from patients with allergic bronchopulmonary aspergillosis (ABPA) or aspergilloma and normal sera were analyzed for specific antibodies by Western (immuno-) blotting with Aspergillus fumigatus antigens transferred electrophoretically onto polyvinylidene difluoride membranes. Western blot analysis demonstrated con...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.27.6.1312-1316.1989
更新日期:1989-06-01 00:00:00
abstract::Several antimicrobial agents used in selective media for the isolation of Arcobacter were found to be inhibitory to strains belonging to this genus. All three species tested were susceptible to colistin and rifampin at concentrations used in selective media. Arcobacter skirrowii was the most susceptible species. 5-Flu...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.39.4.1654-1656.2001
更新日期:2001-04-01 00:00:00
abstract::Automated DNA sequencing was used to characterize mutations associated with rifampin resistance in a 69-bp region of the gene, rpoB, encoding the beta subunit of RNA polymerase in Mycobacterium tuberculosis. The data confirmed that greater than 90% of rifampin-resistant strains have sequence alterations in this region...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.32.4.1095-1098.1994
更新日期:1994-04-01 00:00:00
abstract::Hepatitis C virus (HCV) infection is an increasing health problem worldwide. Quantitative assays for HCV viral load are valuable in predicting response to therapy and for following treatment efficacy. Unfortunately, most quantitative tests for HCV RNA are limited by poor sensitivity. We have developed a convenient, hi...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.42.9.4130-4136.2004
更新日期:2004-09-01 00:00:00