Residual radiation effect in the murine hematopoietic stem cell compartment.

Abstract:

:Stem cells surviving radiation injury may carry defects which contribute to long-term effects. The ratio of 125-iododeoxyuridine (IUdR) uptake into spleens of lethally irradiated recipient mice between day 3 and day 5 after cell transfusion revealed reduced proliferative ability (PF) of spleen seeding cells in parallel with reduced CFU-S content of donors throughout the study period of one year after 5 Gy gamma irradiation. Additional data aided in evaluating possible mechanisms of PF reduction. Within the range of the graft sizes used, PF was independent of the numbers of cells or CFU-S transfused. Radiation-induced increase in loss of label between days 3 and 5 and prolonged doubling time of proliferating cells indicated enhancement of cell maturation and increase in mitotic cycle time. Increased IUdR uptake per transfused CFU-S suggested extra divisions of transit cells due to insufficiency in the stem cell compartment. It is concluded that persisting defects in surviving stem cells interfere in a complex way with cell proliferation in the hemopoietic system.

journal_name

Radiat Environ Biophys

authors

von Wangenheim KH,Peterson HP,Feinendegen LE

doi

10.1007/BF01211733

subject

Has Abstract

pub_date

1986-01-01 00:00:00

pages

93-106

issue

2

eissn

0301-634X

issn

1432-2099

journal_volume

25

pub_type

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