Residual radiation effect in the murine spleen. In situ measurement by 125-iodo-deoxyuridine retention.

Abstract:

:To investigate whether residual radiation damage in hematopoietic tissue is measurable in situ by a change in cell turnover, the retention of the thymidine analogue 5-(125-I)iodo-2'-deoxyuridine (125-IUdR) following incorporation into DNA of cells in bone marrow and spleen of mice was measured 35 days after 0-500 rad whole body gamma irradiation. In the bone marrow a rapid and a slow turnover component of 125-IUdR retention were found. Both components were almost identical for unirradiated and irradiated mice. In the spleen the 125-IUdR retention curves exhibited three components with increasingly prolonged half-times. In the second component the half-time was longer in irradiated than in unirradiated mice. This was dose-dependent. The increased half-time of 125-IUdR retention in irradiated spleens may be caused by direct cellular damage of long-lived cells (lymphocytes, early hematopoietic progenitor cells) or/and by diminished stimulation of proliferation by microenvironmental or long-range factors.

journal_name

Radiat Environ Biophys

authors

Hübner GE,von Wangenheim KH,Feinendegen LE

doi

10.1007/BF01325468

subject

Has Abstract

pub_date

1982-01-01 00:00:00

pages

195-200

issue

3

eissn

0301-634X

issn

1432-2099

journal_volume

20

pub_type

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