Abstract:
:In situ cDNA:mRNA hybridization is a technique that has been developed for the visualization of cDNA:mRNA hybrids in individual cells. To use this technique to answer questions of regulation in heterogeneous populations of cells in the brain, it must be combined with other procedures allowing for the identification of functional subgroups of neurons. We report here a procedure by which in situ cDNA:mRNA hybridization may be combined with retrograde axonal tracing using the fluorescent tracer fast blue. Using this technique, it now becomes possible to measure mRNA regulation in functional subsets of cells defined by their axonal projections.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Wilcox JN,Roberts JL,Chronwall BM,Bishop JF,O'Donohue Tdoi
10.1002/jnr.490160109subject
Has Abstractpub_date
1986-01-01 00:00:00pages
89-96issue
1eissn
0360-4012issn
1097-4547journal_volume
16pub_type
杂志文章abstract::The mechanisms by which polyglutamine expansion causes common features of neuronal death remain unclear. Here we describe an approach for delivering polyglutamine expansions directly into cultured sympathetic neurons. Glutamine (Q) residues (n = 10, 22, 30) were conjugated with a peptide possessing translocation prope...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20461
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abstract::Brain edema accounts for significant morbidity and mortality in many neurologic conditions such as head trauma, stroke, meningitis, and brain tumor. The water channel aquaporin-4 (AQP4) has been found to be an important determinant of brain water accumulation and clearance of excess brain water. We report the developm...
journal_title:Journal of neuroscience research
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abstract::An immortalized dorsal root ganglion cell line F-11 exhibits many properties of spinal cord neurons and undergoes apoptosis in response to growth factor withdrawal and the exogenous addition of inhibitors of phosphatidylinositol-3-kinase (PI3K). To elucidate the mechanism of apoptosis we generated F-11 clones which ov...
journal_title:Journal of neuroscience research
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490250211
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abstract::We have studied the characteristics of pH(i) regulation at different stages of rat oligodendrocyte differentiation in primary culture. pH(i) was measured at 37 degrees C using the pH-sensitive fluorescent probe BCECF. In immature oligodendrocyte progenitor (OLP), three distinct ionic mechanisms were involved in pH(i) ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
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abstract::The effect of neonatal hyperthyroidism produced by injection of tri-iodothyronine (T3) on myelination and on the microperoxisomal population of the brain was studied in young rats. Data on the lipid composition of myelin show that myelinogenesis starts earlier in treated animals. In coincidence with the early appearan...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490250312
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21236
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22037
更新日期:2009-07-01 00:00:00
abstract::Metabolism of amyloid-beta peptide (A beta) is closely associated with the pathology and etiology of Alzheimer's disease (AD). Neprilysin is the only rate-limiting catabolic peptidase proven by means of reverse genetics to participate in A beta metabolism in vivo. The aim of the present study is to assess whether poss...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
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更新日期:2002-11-01 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
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更新日期:2000-10-15 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21410
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abstract::Adrenal chromaffin cell (ACC) transplants, alone or combined with levodopa treatment, were used in attempted therapy for Parkinson's disease (PD). In a previous study, we demonstrated that levodopa caused chromaffin cell death either by necrosis or by apoptosis in cell culture. Here we report the beneficial effect of ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10456
更新日期:2003-01-01 00:00:00
abstract::Finding inhibitors of the first step of the amyloid cascade, A beta (42) generation, is a major goal of Alzheimer's disease drug development. Two target protease activities, beta-and gamma-secretase, were detected more than 10 years ago but progress in this area has been slow because the enzymes were not identified. U...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.10393
更新日期:2002-11-01 00:00:00
abstract::The number of older people who are suffering from memory impairment is increasing among populations throughout the world. Alzheimer's disease (AD) affects about 5% of people over 65 years old. The hippocampus, a brain area critical for learning and memory, is especially vulnerable to damage in the early stages of AD. ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23319
更新日期:2014-04-01 00:00:00
abstract::Tau microtubule-associated proteins are believed to play a role in regulation of the growth of neuronal processes. In order to study the function of tau protein in vivo, we examined the inhibition of tau expression in PC12 cells by exposing the cells to tau antisense oligodeoxynucleotides. A specific retraction of neu...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490300117
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abstract::Fractionation of muscle microsomes rich in sarcoplasmic reticulum (SR) by isopicnic centrifugation yielded three types of membranes. Heavy (HM), intermediate (IM), and light membranes (LM), with isopicnic points of 38, 33, and 25% w/w sucrose, were rich in terminal cisternae/triads, longitudinal SR, and T-tubules, res...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490330409
更新日期:1992-12-01 00:00:00
abstract::The postmortem stability of brain RNA was measured in rat and human samples for up to 48 hr. Whole rat brains, cooled at a rate approximating that of human brains awaiting autopsy, were collected at intervals from 0 to 48 hr after death and frozen. These samples were compared with freshly obtained, unfrozen rat brains...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490160123
更新日期:1986-01-01 00:00:00
abstract::The neurotrophins are important for their long-term effects on the survival and differentiation of many types of neurons during development. They also appear to protect mature neurons from injury caused by nutrient or oxygen deprivation. More recently, the neurotrophins have been implicated in such short-term processe...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-4547(19971101)50:3<355::AID-JNR
更新日期:1997-11-01 00:00:00
abstract::The analysis of proteins in damaged myelin is crucial to clarify the mechanisms of dysmyelination and demyelination. In the present study, proteomic analysis of myelin using a modified two-dimensional electrophoresis (2-DE) method was carried out to obtain a better understanding of myelin biology. Although standard 2-...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
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更新日期:2008-03-01 00:00:00
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journal_title:Journal of neuroscience research
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490410609
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journal_title:Journal of neuroscience research
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journal_title:Journal of neuroscience research
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更新日期:2000-01-01 00:00:00
abstract::Amyotrophic lateral sclerosis (ALS) is a progressive and fatal disease caused by the selective death of motor neurons. Between 5% and 10% of ALS patients have a genetically inherited form of the disease known as familial ALS (FALS), and approximately 20% of FALS patients have mutations in the SOD1 gene. Although the m...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
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更新日期:2009-03-01 00:00:00
abstract::Nerve growth factor (NGF) is a homodimer that binds to two distinct receptor types, TrkA and p75, to support survival and differentiation of neurons. The high-affinity binding on the cell surface is believed to involve a heteroreceptor complex, but its exact nature is unclear. We developed a heterodimer (heteromutein)...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23116
更新日期:2012-12-01 00:00:00
abstract::The role of the transcription factor AP-1 in regulating D2 receptor transcriptional activity was investigated in D2 receptor expressing neuroblastoma cells, NB41A3, and in non-D2 receptor expressing CHO cells. Deletion of a region containing the putative AP-1 binding site resulted in a significant reduction in the act...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19971001)50:1<23::AID-JNR3
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abstract::The effects of hyposmotic conditions on taurine uptake and release were studied in mice cultured cerebellar granule cells. The effect of DIDS (4,4'-diisothiocyanatostilbene-2,2'-disulfonate) and of the divalent cations Mg++ and Mn++ on the hyposmolarity-induced changes in these parameters was investigated. Mg++ (20 mM...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490300409
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abstract::Low density dissociated cultures of embryonic rat hippocampal cells were used to study the effects of neurotrophin-3 (NT-3) on neuronal morphogenesis. The results obtained indicate that NT-3 enhances neurite outgrowth and branching; this is a dose-dependent effect, detected in approximately 50% of the neurons, and pre...
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pub_type: 杂志文章
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
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更新日期:1992-06-01 00:00:00
abstract::The osteoinductive capacity of biological noncellular material has been widely recognized. Studies using bone morphogenetic proteins and acellular bone matrix demonstrate that host mesenchymal cells can be readily transformed into osteoprogenitor cells. The current study sought to determine whether another biological ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
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更新日期:2015-03-01 00:00:00