Synergistic myelopoietic actions in vivo after administration to mice of combinations of purified natural murine colony-stimulating factor 1, recombinant murine interleukin 3, and recombinant murine granulocyte/macrophage colony-stimulating factor.

abstract：:Synaptic vesicles release neurotransmitter by following a process of vesicle docking and exocytosis. Although these steps are well established, it has been difficult to observe and measure these rates directly in living synapses. Here, by combining the direct imaging of single synaptic vesicles and synaptic ribbons, I...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zenisek D

更新日期：2008-03-25 00:00:00

abstract：:Coxsackievirus causes severe disease in adult mice subjected to sustained post-weaning undernutrition (marasmus). Virus-infected marasmic mice have an increased incidence of mortality, severe lesions, and elevated and persistent viral in target organs. Transfer of immune lymphoid cells 30 min or 24 hr after viral chal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Woodruff JF,Woodruff JJ

更新日期：1971-09-01 00:00:00

abstract：:We have examined the use of our bis(sulfonamide) diol ligand (1) in the asymmetric addition of phenyl groups to cyclic alpha,beta-unsaturated ketones. Good to excellent enantioselectivities have been obtained with cyclic enones bearing alkyl substituents in the 2 position (71-97% enantiomeric excess). Furthermore, exc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li H,García C,Walsh PJ

更新日期：2004-04-13 00:00:00

abstract：:The skeletal remains of a diminutive small-brained hominin found in Late Pleistocene cave deposits on the island of Flores, Indonesia were assigned to a new species, Homo floresiensis [Brown P, et al. (2004) A new small-bodied hominin from the Late Pleistocene of Flores, Indonesia. Nature 431: 1055-1061]. A dramatical...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gordon AD,Nevell L,Wood B

更新日期：2008-03-25 00:00:00

abstract：:Transgenic mice have been used to address the issue of the oncogenic potential of mutant guanine nucleotide stimulatory factor (Gs) alpha subunit in the thyroid gland. The expression of the mutant Arg-201-->His Gs alpha subunit transgene has been directed to murine thyroid epithelial cells by bovine thyroglobulin prom...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Michiels FM,Caillou B,Talbot M,Dessarps-Freichey F,Maunoury MT,Schlumberger M,Mercken L,Monier R,Feunteun J

更新日期：1994-10-25 00:00:00

abstract：:NADH:quinone oxidoreductase (complex I) plays a pivotal role in cellular energy production. It employs a series of redox cofactors to couple electron transfer to the generation of a proton-motive force across the inner mitochondrial or bacterial cytoplasmic membrane. Complex I contains a noncovalently bound flavin mon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yakovlev G,Reda T,Hirst J

更新日期：2007-07-31 00:00:00

abstract：:Genomic and cDNA clones for the human nerve growth factor receptor have been used in conjunction with somatic cell hybrid analysis and in situ hybridization to localize the nerve growth factor receptor locus to human chromosome region 17q12-q22. Additionally, part, if not all, of the nerve growth factor receptor locus...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huebner K,Isobe M,Chao M,Bothwell M,Ross AH,Finan J,Hoxie JA,Sehgal A,Buck CR,Lanahan A

更新日期：1986-03-01 00:00:00

abstract：:Junctions that mediate excitation-contraction (e-c) coupling are formed between the sarcoplasmic reticulum (SR) and either the surface membrane or the transverse (T) tubules in normal skeletal muscle. Two structural components of the junctions, the feet of the SR and the tetrads of T tubules, have been identified resp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Takekura H,Nishi M,Noda T,Takeshima H,Franzini-Armstrong C

更新日期：1995-04-11 00:00:00

abstract：:An explicitly polarizable force field based exclusively on quantum data is applied to calculations of relative binding affinities of ligands to proteins. Five ligands, differing by replacement of an atom or functional group, in complexes with three serine proteases-trypsin, thrombin, and urokinase-type plasminogen act...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Khoruzhii O,Donchev AG,Galkin N,Illarionov A,Olevanov M,Ozrin V,Queen C,Tarasov V

更新日期：2008-07-29 00:00:00

abstract：:Object recognition is challenging because the same object can produce vastly different images, mixing signals related to its identity with signals due to its image attributes, such as size, position, rotation, etc. Previous studies have shown that both signals are present in high-level visual areas, but precisely how ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ratan Murty NA,Arun SP

更新日期：2018-04-03 00:00:00

abstract：:The lactose permease (LacY) of Escherichia coli, a paradigm for the major facilitator superfamily, catalyzes the coupled stoichiometric translocation of a galactopyranoside and an H(+) across the cytoplasmic membrane. To catalyze transport, LacY undergoes large conformational changes that allow alternating access of s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Serdiuk T,Madej MG,Sugihara J,Kawamura S,Mari SA,Kaback HR,Müller DJ

更新日期：2014-04-22 00:00:00

abstract：:Immunoglobulin sequences were compared by using a technique that takes into account the dissimilarity in physicochemical properties of amino acids. Exterior residues showed greater structural variability than interior residues. High structural variability was found at positions known from crystallographic studies to b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Padlan EA

更新日期：1977-06-01 00:00:00

abstract：:We report the direct measurement of thermodynamic properties of colloidal hard spheres by optical microscopy. By using confocal microscopy, we obtain three-dimensional snapshots of a colloidal hard-sphere suspension over a wide range of densities. From these snapshots, the available volume to insert an additional sphe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dullens RP,Aarts DG,Kegel WK

更新日期：2006-01-17 00:00:00

abstract：:Direct visualization of genomic loci in the 3D nucleus is important for understanding the spatial organization of the genome and its association with gene expression. Various DNA FISH methods have been developed in the past decades, all involving denaturing dsDNA and hybridizing fluorescent nucleic acid probes. Here w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Deng W,Shi X,Tjian R,Lionnet T,Singer RH

更新日期：2015-09-22 00:00:00

abstract：:The kinetics of charge transfer depend crucially on the dielectric reorganization of the medium. In enzymatic reactions that involve charge transfer, atomic dielectric response of the active site and of its surroundings determines the efficiency of the protein as a catalyst. We report direct spectroscopic measurements...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mertz EL,Krishtalik LI

更新日期：2000-02-29 00:00:00

abstract：:Enolase genes from land plants and apicomplexa (intracellular parasites, including the malarial parasite, Plasmodium) share two short insertions. This observation has led to the suggestion that the apicomplexan enolase is the product of a lateral transfer event involving the algal endosymbiont from which the apicomple...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Keeling PJ,Palmer JD

更新日期：2001-09-11 00:00:00

abstract：:To gain further insight into the mode of action of S-locus receptor kinase (SRK), a receptor-like kinase involved in the self-incompatibility response in Brassica, different recombinant SRK proteins have been expressed in a membranous environment using the insect cell/baculovirus system. Recombinant SRK proteins exhib...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Giranton JL,Dumas C,Cock JM,Gaude T

更新日期：2000-03-28 00:00:00

abstract：:Invariant natural killer T (iNKT) cells recognize self lipid antigens presented by CD1d molecules. The nature of the self-antigens involved in the development and maturation of iNKT cells is poorly defined. Lysophospholipids are self-antigens presented by CD1d that are generated through the action of phospholipases A1...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Paduraru C,Bezbradica JS,Kunte A,Kelly R,Shayman JA,Veerapen N,Cox LR,Besra GS,Cresswell P

更新日期：2013-03-26 00:00:00

abstract：:By comparing the amino acid sequences of 11 mammalian and 1 avian prion proteins (PrP), structural analyses predicted four alpha-helical regions. Peptides corresponding to these regions of Syrian hamster PrP were synthesized, and, contrary to predictions, three of the four spontaneously formed amyloids as shown by ele...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gasset M,Baldwin MA,Lloyd DH,Gabriel JM,Holtzman DM,Cohen F,Fletterick R,Prusiner SB

更新日期：1992-11-15 00:00:00

abstract：:Nascent precursors of phosphatidylinositol-glycan (PI-G)-linked membrane proteins contain a hydrophobic COOH-terminal sequence of 15-30 residues that is eliminated during processing to yield a newly exposed COOH terminus to which the PI-G moiety is added. There is no consensus as to the primary structure of the termin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Micanovic R,Kodukula K,Gerber LD,Udenfriend S

更新日期：1990-10-01 00:00:00

abstract：:Autoantibodies directed against the cell surface receptors for insulin are found in some patients with extreme insulin resistance. These antibodies specifically inhibit the binding of insulin to its receptor. A purified IgG fraction from one patient's plasma was labeled with 125I. The 125I-labeled antireceptor antibod...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jarrett DB,Roth J,Kahn CR,Flier JS

更新日期：1976-11-01 00:00:00

abstract：:Proactive policing, the strategic targeting of people or places to prevent crimes, is a well-studied tactic that is ubiquitous in modern law enforcement. A 2017 National Academies of Sciences report reviewed existing literature, entrenched in deterrence theory, and found evidence that proactive policing strategies can...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Del Toro J,Lloyd T,Buchanan KS,Robins SJ,Bencharit LZ,Smiedt MG,Reddy KS,Pouget ER,Kerrison EM,Goff PA

更新日期：2019-04-23 00:00:00

abstract：:Epstein-Barr virus (EBV) latent infection membrane protein 1 (LMP1)-induced NF-kappaB activation is important for infected cell survival. LMP1 activates NF-kappaB, in part, by engaging tumor necrosis factor (TNF) receptor-associated factors (TRAFs), which also mediate NF-kappaB activation from LTbetaR and CD40. LTbeta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luftig M,Yasui T,Soni V,Kang MS,Jacobson N,Cahir-McFarland E,Seed B,Kieff E

更新日期：2004-01-06 00:00:00

abstract：:To define the T-cell receptor signal transduction motif, we have transfected human and murine T-cell lines with a chimeric receptor consisting of the extracellular and transmembrane domains of human CD8 alpha and the membrane-proximal portion of CD3 zeta containing at its C terminus either an 18-amino acid segment (NQ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Koyasu S,Tse AG,Moingeon P,Hussey RE,Mildonian A,Hannisian J,Clayton LK,Reinherz EL

更新日期：1994-07-05 00:00:00

abstract：:Embryonal rhabdomyosarcoma (ERMS) is a common pediatric malignancy of muscle, with relapse being the major clinical challenge. Self-renewing tumor-propagating cells (TPCs) drive cancer relapse and are confined to a molecularly definable subset of ERMS cells. To identify drugs that suppress ERMS self-renewal and induce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen EY,DeRan MT,Ignatius MS,Grandinetti KB,Clagg R,McCarthy KM,Lobbardi RM,Brockmann J,Keller C,Wu X,Langenau DM

更新日期：2014-04-08 00:00:00

abstract：:We report the discovery of mammalian tribosphenic teeth from the basal Cenomanian of southwestern France that we refer to a new primitive marsupial-like form identified as a basal taxon of Marsupialiformes, a new clade recognized here to include the crown group Marsupialia and primitive stem lineages more closely rela...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vullo R,Gheerbrant E,de Muizon C,Néraudeau D

更新日期：2009-11-24 00:00:00

abstract：:Three-dimensional microfluidic systems were fabricated and used to pattern proteins and mammalian cells on a planar substrate. The three-dimensional topology of the microfluidic network in the stamp makes this technique a versatile one with which to pattern multiple types of proteins and cells in complex, discontinuou...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chiu DT,Jeon NL,Huang S,Kane RS,Wargo CJ,Choi IS,Ingber DE,Whitesides GM

更新日期：2000-03-14 00:00:00

abstract：:T cells react to extremely small numbers of activating agonist peptides. Spatial organization of T-cell receptors (TCR) and their peptide-major histocompatibility complex (pMHC) ligands into microclusters is correlated with T-cell activation. Here we have designed an experimental strategy that enables control over the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Manz BN,Jackson BL,Petit RS,Dustin ML,Groves J

更新日期：2011-05-31 00:00:00

abstract：:Trypanosomatid RNA editing is a unique process and essential for these organisms. It therefore represents a drug target for a group of protozoa that includes the causative agents for African sleeping sickness and other devastating tropical and subtropical diseases. Here, we present drug-like inhibitors of a key enzyme...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Amaro RE,Schnaufer A,Interthal H,Hol W,Stuart KD,McCammon JA

更新日期：2008-11-11 00:00:00

abstract：:Early embryos of many organisms develop outside the mother and are immediately confronted with myriads of potential colonizers. How these naive developmental stages control and shape the bacterial colonization is largely unknown. Here we show that early embryonic stages of the basal metazoan Hydra are able to control ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fraune S,Augustin R,Anton-Erxleben F,Wittlieb J,Gelhaus C,Klimovich VB,Samoilovich MP,Bosch TC

更新日期：2010-10-19 00:00:00