Abstract:
OBJECTIVE:Heart diseases are common in the offspring of gestational diabetic mother (ODM). Defects in mitochondrial metabolism and autophagy may, in part, be responsible for the adverse structural and functional alterations in the heart. The principal objective of this study was to investigate cardiac mitochondrial respiration and autophagy in male and female offspring of diabetic pregnancy at two different developmental stages of life, weaning and adult. METHODS:Male and female offspring of rats with streptozotocin-induced gestational diabetes were used for the study and compared with offspring of control (non-diabetic) mother (OCM) rats. High-resolution respirometry was used to measure substrate-mediated respiration in mitochondria isolated from ventricular tissues of ODM and OCM. Expression of proteins associated with autophagy and oxidative stress was examined by western blot analysis. RESULTS:Mitochondrial complex I and complex II respiration was significantly reduced in adult male ODM while it was unaltered or less affected in weaning male, adult and weaning female ODM. Elevated autophagy was observed in adult male but not in adult female ODM. Expression of oxidative stress markers was observed to be similar in all the groups. CONCLUSION:Altered cardiac mitochondrial respiration and autophagy were observed in adult male ODM compared to OCM, while the male and female offspring at weaning stage were less affected. The results of the study show that maternal hyperglycemia affects mitochondrial respiration and autophagy in the ODM heart, which may potentially be responsible for the cardiovascular complications observed in adult life. This article is protected by copyright. All rights reserved.
journal_name
IUBMB Lifejournal_title
IUBMB lifeauthors
Rajendran RS,Jayakumari NR,Sivasailam A,Reghuvaran AC,Pillai VV,Karunakaran J,Sreelatha HV,Manjunatha S,Gopala Sdoi
10.1002/iub.2449subject
Has Abstractpub_date
2021-01-22 00:00:00eissn
1521-6543issn
1521-6551pub_type
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